• Title/Summary/Keyword: gyrA

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Occurrence of Bacterial Stem Rot of Ranunculus asiaticus Caused by Pseudomonas marginalis in Korea

  • Li, Weilan;Ten, Leonid N.;Kim, Seung-Han;Lee, Seung-Yeol;Jung, Hee-Young
    • Research in Plant Disease
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    • v.24 no.2
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    • pp.138-144
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    • 2018
  • In December 2016, stem rot symptoms were observed on Persian buttercup (Ranunculus asiaticus) plants in Chilgok, Gyeongbuk, Korea. In the early stage of the disease, several black spots appeared on the stem of infected plants. As the disease progressed, the infected stem cleaved and wilted. The causal agent was isolated from a lesion and incubated on Reasoner's 2A (R2A) agar at $25^{\circ}C$. Total genomic DNA was extracted for phylogenetic analysis. Based on the 16S rRNA gene analysis, the isolated strain was found to belong to the genus Pseudomonas. To identify the isolated bacterial strain at the species level, the nucleotide sequences of the gyrase B (gyrB) and RNA polymerase D (rpoD) genes were obtained and compared with the sequences in the GenBank database. As the result, the causal agent of the stem rot disease was identified as Pseudomonas marginalis. To determine the pathogenicity of the isolated bacterial strain, it was inoculated into the stem of healthy R. asiaticus plant, the inoculated plant showed a lesion with the same characteristics as the naturally infected plant. Based on these results, this is the first report of bacterial stem rot on R. asiaticus caused by P. marginalis in Korea.

Norfloxacin Resistance Mechanism of E. coli 11 and E. coli 101-Clinical Isolates of Escherichia coli in Korea

  • Kim, Kyung-Soon;Lee, Soon-Deuk;Lee, Yeon-Hee
    • Archives of Pharmacal Research
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    • v.19 no.5
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    • pp.353-358
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    • 1996
  • E. coli 11 and E. coli 101, clinical isolates of Escherichia coli were resistant to various quinolones, especially MICs to norfloxacin of both strains were higher than 100 mg/ml. In the presence of carbonyl cyanide m-chlorophenylhydrazone, a proton gradient uncoupler, norfloxacin uptake in both strains was increased, suggesting that an efflux system play an important role in the norfloxacin resistance. Outer membrane proteins of the susceptible and resistant strains which could affect the route of norfloxacin entry into cells were different. When quinolone resistance determining region(QRDR) of gyrA was amplified using PCR and cut with Hinf I, QRDR in the susceptible strain yielded two fragments while QRDRs in E. coli 11 and E. coli 101 yielded only one uncut fragment. When DNA sequence of QRDR was analyzed, there were two mutations as Ser-83 and Asp-87 in both resistant strains. these residues were changed to Leu-83 and Asn-87, respectively. These results showed that the norfloxacin resistance of E. coli 11 and E. coli 101 was resulted from multiple changes-an altered DNA gyrase A subunit, a change in route of drug entry, and reduction in quinolone concentration inside cells due to an efflux system.

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Non-axisymmetric Features of Dwarf Elliptical Galaxies

  • Kwak, Sungwon;Kim, Woong-Tae;Rey, Soo-Chang;Kim, Suk
    • The Bulletin of The Korean Astronomical Society
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    • v.41 no.1
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    • pp.39.3-39.3
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    • 2016
  • About one tenth of dwarf elliptical galaxies found in the Virgo cluster have a disk component, and some of them even possess substructures such as bars, lens, and spiral arms. We use N-body simulations to study the formation of these non-axisymmetric features in disky dwarf elliptical galaxies. By mimicking VCC 856, a bulgeless dwarf galaxy with embedded faint spiral arms, we construct 11 sets of initial conditions with slight dynamical variations based on observational data. Our standard model starts slowly to form a bar at ~3 Gyr and then undergoes buckling instability that temporarily weakens the bar although the bar strength continues to grow afterward. We find 9 of our models are unstable to bar formation and undergo buckling instability. This suggests that disky dwarf elliptical galaxies are intrinsically unstable to form bars, accounting for a population of barred dwarf galaxies in the outskirts of the Virgo cluster. To understand the origin of the faint grand-design spiral arms, we additionally construct 6 sets of models that undergo tidal interactions with their neighbors. We find that faint spiral arms consistent with observations develop when tidal forcing is relatively weak although strong encounter still results in bar formation. We discuss our results in light of the dynamical evolution of dwarf elliptical galaxies including mergers.

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Kinematic properties of the Ursa Major Cluster

  • Kim, YoungKwang;Lee, Young Sun;Beers, Timothy C.
    • The Bulletin of The Korean Astronomical Society
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    • v.40 no.2
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    • pp.30.3-31
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    • 2015
  • We present a kinematic analysis of 172 likely member galaxies of the Ursa Major Cluster. In order to understand the dynamical state of the cluster, we investigate the correlation of the cluster morphology with rotation, the velocity dispersion profile, and the rotation amplitude parallel to the global rotation direction. Both the minor axis and the rotation are very well-aligned with the global rotation axis in the outer region at half radius (> 0.5 $R_{max}$), but not in the inner region. The cluster exhibits low velocity dispersion and rotation amplitude profiles in the inner region, but higher in the outer. Both profiles exhibit outwardly increasing trends, suggesting an inside-out transfer of angular momentum of dark matter via violent relaxation, as revealed by a recent off-axis major-merging simulation. From Dressler-Schectman plots in the plane of galactic positions, and velocity versus position angle of galaxy, we are able to divide the Ursa Major Cluster into two substructures: Ursa Major South (UMS) and Ursa Major North (UMN). We derive a mass of $3.2{\times}10^{14}M_{\odot}$ for the cluster through the two-body analysis by the timing argument with the distance information (37 for UMN and 36 for UMS) and the spin parameter of ${\lambda}=0.049$. The two substructures appear to have passed each other 4.4 Gyr ago and are moving away to the maximum separation.

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A New Galaxy Classification Scheme in the WISE Color-Luminosity Diagram

  • Lee, Gwang-Ho;Sohn, Jubee;Lee, Myung Gyoon
    • The Bulletin of The Korean Astronomical Society
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    • v.38 no.2
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    • pp.49.1-49.1
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    • 2013
  • We present a new galaxy classification scheme in the Wide-field Infrared Survey Explorer (WISE) [$3.4{\mu}m$]-[$12{\mu}m$] color versus $12{\mu}m$ luminosity diagram. In this diagram, galaxies can be classified into three groups in different evolutionary stages. Late-type galaxies are distributed linearly along "MIR star-forming sequence" identified by Hwang et al. (2012). Some early-type galaxies show another sequence at [3.4]-[12] $(AB){\simeq}-2.0$, and we call this 'MIR blue sequence'. They are quiescent systems with old stellar population older than 10 Gyr. Between the MIR star-forming sequence and the MIR blue sequence, some early- and late-type galaxies are sparsely distributed, and we call these galaxies 'MIR green cloud galaxies'. Interestingly, both MIR blue sequence galaxies and MIR green cloud ones lie on the red sequence in the optical color-magnitude diagram. However, MIR green cloud galaxies have lower stellar masses and younger stellar populations (smaller $D_n4000$) than MIR blue sequence galaxies, suggesting that MIR green cloud galaxies are in the transition stage from MIR star-forming sequence galaxies to MIR blue sequence ones. We present differences in various galaxy properties between the three MIR classes using a multi-wavelength data, combined with the WISE and Sloan Digital Sky Survey Data Release 10, of local (0.03 < z < 0.07) galaxies.

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DARK MATTER CONTENT IN GLOBULAR CLUSTER NGC 6397

  • Shin, Jihye;Kim, Sungsoo S.;Lee, Young-Wook
    • Journal of The Korean Astronomical Society
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    • v.46 no.4
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    • pp.173-181
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    • 2013
  • We trace the dynamical evolution of dark matter (DM) content in NGC 6397, one of the native Galactic globular clusters (GCs). The relatively strong tidal field (Galactocentric radius of ~ 6 kpc) and short relaxation timescale (~0.3 Gyr) of the cluster can cause a significant amount of DM particles to evaporate from the cluster in the Hubble time. Thus, the cluster can initially contain a non-negligible amount of DM. Using the most advanced Fokker-Planck (FP) method, we calculate the dynamical evolution of GCs for numerous initial conditions to determine the maximum initial DM content in NGC 6397 that matches the present-day brightness and velocity dispersion profiles of the cluster. We find that the maximum allowed initial DM mass is slightly less than the initial stellar mass in the cluster. Our findings imply that NGC 6397 did not initially contain a significant amount of DM, and is similar to that of NGC 2419, the remotest and the most massive Galactic GC.

Biological Control of Strawberry Fusarium Wilt Caused by Fusarium oxysporum f. sp.fragariae Using Bacillus velezensis BS87 and RK1 Formulation

  • Nam, Myeong-Hyeon;Park, Myung-Soo;Kim, Hong-Gi;Yoo, Sung-Joon
    • Journal of Microbiology and Biotechnology
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    • v.19 no.5
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    • pp.520-524
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    • 2009
  • Two isolates, Bacillus sp. BS87 and RK1, selected from soil in strawberry fields in Korea, showed high levels of antagonism towards Fusarium oxysporum f. sp. fragariae in vitro. The isolates were identified as B. velezensis based on the homology of their gyrA sequences to reference strains. BS87 and RK1 were evaluated for control of Fusarium wilt in strawberries in pot trials and field trials conducted in Nonsan, Korea. In the pot trials, the optimum applied concentration of BS87 and RK1 for pre-plant root-dip application to control Fusarium wilt was $10^5$ and $10^6$ colony-forming units (CFU)/ml, respectively. Meanwhile, in the 2003 and 2005 field trials, the biological control efficacies of formulations of RK1 were similar to that of a conventional fungicide (copper hydroxide) when compared with a non-treated control. The RK1 formulation was also more effective than BS87 in suppressing Fusarium wilt under field conditions. Therefore, the results indicated that formulations of B. velezensis BS87 and RK1 may have potential to control Fusarium wilt in strawberries.

Research on Solar System Small Bodies using the Korean Small Telescopes Network

  • Ishiguro, Masateru
    • The Bulletin of The Korean Astronomical Society
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    • v.44 no.2
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    • pp.60.4-60.4
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    • 2019
  • Small bodies in the solar system are pristine leftovers of planetesimals since the formation epoch (~4.6 Gyr ago). After the formation, icy planetesimals have been preserved in the distant cold place beyond 30 au (i.e., Trans-Neptunian region) until recently without any catastrophic processes but have just been injected into inner region (<~5 au from the Sun) to be observed as comets. On the contrary, asteroids are rocky primitive objects (although some of them contains icy volatiles) distributing in the mainbelt between Mars and Jupiter orbits. Because of frequent encounters in the mainbelt, asteroids have experienced a number of repeated impacts until the present day. Namely, it is important to investigate thermal alternation process of cometary volatiles and refractories in the solar radiation field, whereas collisional and subsequence phenomena of asteroidal bodies. Although recent spacecraft observations revealed the physical natures on the surfaces of comets and asteroids, their interiors still remain largely unexplored. It is likely that a sudden brightening of a comet is associated with rapid sublimation of internal CO and CO2 or phase transition of amorphous H2O. An episodic dust ejection from an asteroid is causally related to an impact among asteroids, sudden sublimation of remaining subsurficial volatiles, etc. Because these transient phenomena provide rare opportunities to investigate their interiors, immediate observations using any optical instruments are particular important. In my presentation, I will review some examples of such transient phenomena in the solar system and propose possible collaborative research using the Korean Small Telescope Network.

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Demographics of Isolated Galaxies along the Hubble Sequence

  • Kim, Hong-Geun;Park, Jongwon;Seo, Seong-Woo;Yi, Sukyoung K.
    • The Bulletin of The Korean Astronomical Society
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    • v.40 no.1
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    • pp.73.1-73.1
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    • 2015
  • Isolated galaxies in low-density regions are significant in the sense that they are least affected by the hierarchical pattern of galaxy growth and interactions with perturbers at least for the last few Gyr. To form a comprehensive picture of the star formation history of isolated galaxies, we construct a catalog of isolated galaxies and their comparison sample in relatively denser environments. The galaxies are drawn from SDSS DR7 in the redshift range of 0.025 < z < 0.044. We performed visual inspection and classified their morphology following the Hubble classification scheme. We have investigated the color-magnitude diagram and found elliptical and unbarred spiral galaxies in isolated systems are relatively fainter and bluer than those in denser regions. For the spectroscopic study, we make use of the OSSY catalog (Oh et al. 2011). Our analysis on the absorption-line properties based on the comparison with stellar population models suggests that isolated elliptical galaxies are likely to be younger and metal poorer, while isolated Sc-type galaxies seem to have older luminosity-weighted ages, than their high-density counterpart. In addition, according to the BPT diagnostics, early-type galaxies among isolated galaxies are rather evenly classified into star forming, composite, Seyfert and LINER, whereas their comparisons are mainly populated in the LINER region. On the other hand, late-type galaxies do not show any prominent difference. We discuss the evolutionary histories of isolated galaxies in the context of the standard ${\Lambda}CDM$ cosmology.

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A PHOTOMETRIC STUDY OF FIVE OPEN CLUSTERS IN THE SDSS

  • Ryu, Jin-Hyuk;Lee, Myung-Gyoon
    • Journal of The Korean Astronomical Society
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    • v.44 no.5
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    • pp.177-193
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    • 2011
  • We present a photometric study of five open clusters (Czernik 5, Alessi 53, Berkeley 49, Berkeley 84, and Pfleiderer 3) in the Sloan Digital Sky Survey. The position and size of these clusters are determined using the radial number density profiles of the stars, and the member stars of the clusters are selected using the proper motion data in the literature. We estimate the reddening, distance and age of the clusters based on the isochrone fitting in the color-magnitude diagram. The foreground reddenings for these clusters are estimated to be E(B - V ) = 0.71 - 1.55 mag. The distances to these clusters are derived to be 2.0 - 4.4 kpc, and their distances from the Galactic center range from 7.57 kpc to 12.35 kpc. Their ages are in the range from 250 Myr to 1 Gyr. Berkeley 49 and Berkeley 84 are located in the Orion spur, Czernik 5 is in the Perseus arm, and Pfleiderer 3 and Alessi 53 are located beyond the Perseus arm.