• 한국식생활문화학회지
• /
• 제38권6호
• /
• pp.434-441
• /
• 2023

This study investigated quality characteristics and antioxidant activity of beetroots after blanching. Beetroots were blanched in distilled water, 2% NaCl water, and 2% citric acid water at 100℃ for 3 minutes (the blanched group). The moisture content was highest in the control (CON) at 91.30% (p<0.05), and cooking loss was lowest in the water-blanched beetroot (BW) at 5.39% (p<0.01). Chromaticity decreased after blanching compared to CON (p<0.001). Total polyphenol contents (TPC) and total flavonoid contents (TFC) decreased after blanching, and as a result of comparing the True retention (TR) of the blanching treatment group, BW had the highest with TPC TR 91.22% and TFC TR 70.51%. DPPH and ABTS+radical scavenging activities were highest in the CON, and in the blanching group BW was highest scavenging activity. The total number of microorganisms in the CON group was 2.97 log CFU/g, whereas no microorganisms were detected in the blanched groups. Therefore, this study, blanching in water without additives is the most appropriate method for preserving physiologically active substances and nutrients in beetroots and inhibiting microbial growth.

• 한국미생물·생명공학회지
• /
• 제37권4호
• /
• pp.322-332
• /
• 2009

Actinomycetes 500계통 중 식물병원성 곰팡이(Alternaria alternata, Botryotinia fuckeliana, Colletotrichum acutatum, Colletotrichum gleosporioides, Corticium sasaki, Cylindrocarpon destructans, Fusarium oxysporium, Magnaporthe grisea, Phytophthora infestans, Phytium ultimum, Thanatephorus cucumeri)저해하는 균주를 선발하였다. CNU-A91231(Korea Agricultural Culture Collection #20938)이 식물병원성 곰팡이에 대한 억제 활성이 강한 것으로 나타났으며 16s rDNA sequence를 통해 Streptomyces griseofuscus로 동정 하였다. S. griseofuscus CNUA91231의 효율적인 배양을 위해 간단하고 실용적인 배지를 개발하였다. 이 균주의 최적 성장조건은 28, pH 6에서 aeration 조건이었다. Alanine, glutamine, proline, ammonium ion은 좋은 질소원이었다. 당밀에 Na, Cl, Ca, P, K, Mg를 첨가하였을 때는 S. griseofuscus.의 성장을 증가시키지 못하였다. 아미노산을 함유한 비료를 사용하였을 때 이 균주의 성장을 증가시켰다. 최적의 배지로서 당밀 + 1% glutamate 부산물 분말로 결정하였다. 최적 배지에서 S. griseofuscus의 배양은 이 균주의 antifungal 활성에 영향을 주지 않았다. 본 연구에서 연구된 bacterium과 배지가 작물재배에 생물학적 항 곰팡이 제재로 이용될 것으로 기대된다.

• 농약과학회지
• /
• 제12권2호
• /
• pp.168-176
• /
• 2008

최근 국내의 벼 포장에서는 키다리병의 발생률이 크게 증가하고 있다. 본 실험에서는 키다리병 방제에 효과적으로 사용할 수 있는 살균제를 검정하고자, 5가지의 검정법을 이용하여 17종 살균제의 효과를 조사하였다. Prochloraz는 5가지의 검정법 모두에서 가장 우수한 효과를 나타내었지만, 동일한 ergosterol 생합성을 저해하는 다른 살균제 중에서 온실 검정과 Komada 배지상에서의 균검출율을 조사한 $500\;{\mu}g\;mL^{-1}$의 hexaconazole과 triflumizole을 제외하고 나머지의 효과는 매우 저조하였다. Benomyl과 benomyl/thiram의 혼합제는 100과 $500\;{\mu}g\;mL^{-1}$를 처리한 포자발아와 pouch 검정법에서 우수한 효과를 나타내었다. Trifloxystrobin과 kresoxim-methyl의 $20\;{\mu}g\;mL^{-1}$에서 소독한 종자는 균검출율이 크게 낮아졌으며, thiram 역시 $100\;{\mu}g\;mL^{-1}$의 소독 처리에서 병원균이 검출되는 종자의 비율이 감소하였다. Fludioxonil 역시 포자발아, Komada 배지에서의 균검출율, pouch 검정 등에서 우수한 효과를 보였다. 이상의 결과는 살균제의 작용기작과 특성에 맞추어 검정 방법을 결정하는 것이 중요하다는 사실을 보여주고 있다고 생각한다.

• Asian Pacific Journal of Cancer Prevention
• /
• 제13권11호
• /
• pp.5839-5842
• /
• 2012

Breast cancer is the most commonly diagnosed cancer in women in the world and is one of the leading causes of death due to cancer. Health benefits have been linked to additive and synergistic combinations of phytochemicals in fruits and vegetables. Nigella sativa has been shown to possess anti-carcinogenic activity, inhibiting growth of several cancer cell lines in vitro. However, the molecular mechanisms of the anti-cancer properties of Nigella sativa phytochemical extracts have not been completely understood. Our data showed that Nigella sativa extracts significantly inhibited human breast cancer MDA-MB-231 cell proliferation at doses of $2.5-5{\mu}g/mL$ (P<0.05). Apoptotic induction in MDA-MB-231 cells was observed in a dose-dependent manner after exposure to Nigella sativa extracts for 48 h. Real time PCR and flow cytometry analyses suggested that Nigella sativa extracts possess the ability to suppress the proliferation of human breast cancer cells through induction of apoptosis.

• BMB Reports
• /
• 제48권3호
• /
• pp.166-171
• /
• 2015

We previously demonstrated that resveratrol and clofarabine elicited a marked cytotoxicity on malignant mesothelioma (MM) MSTO-211H cells but not on the corresponding normal mesothelial MeT-5A cells. Little is known of the possible molecules that could be used to predict preferential chemosensitivity on MSTO-211H cells. Resveratrol and clofarabine induced downregulation of Mcl-1 protein level in MSTO-211H cells. Treatment of cells with cycloheximide in the presence of proteasome inhibitor MG132 suggested that Mcl-1 protein levels were regulated at the post-translational step. The siRNA-based knockdown of Mcl-1 in MSTO-211H cells triggered more growth-inhibiting and apoptosis-inducing effects with the resultant cleavages of procaspase-3 and its substrate PARP, increased caspase-3/7 activity, and increased percentage of apoptotic propensities. However, the majority of the observed changes were not shown in MeT-5A cells. Collectively, these studies indicate that the preferential activation of caspase cascade in malignant cells might have important applications as a therapeutic target for MM.

• Journal of Dairy Science and Biotechnology
• /
• 제32권1호
• /
• pp.1-6
• /
• 2014

Probiotics have been extensively studied for their beneficial effects on human health. In particular, Lactobacillus and Bifidobacterium strains have gained considerable attention as major groups of probiotic bacteria that improve gastrointestinal health. However, emerging evidence suggests that probiotics offer benefits beyond those observed in the gut recent studies suggest that probiotics and/or their components exert favorable effects on alcoholic liver disease (ALD) pathogenesis such as decreasing intestinal permeability, inhibiting pathogenic bacteria growth, increasing the activity of alcohol metabolism enzymes, modulating the adaptive immune system, and suppressing fatty acid synthesis genes. In this review, we discuss the results of in vivo and in vitro studies that have examined the use of probiotics to prevent ALD, primarily focusing on those that explore the cellular and molecular mechanisms underlying the activities of promising probiotic strains. The evidence presented in this review could help in screening for probiotic strains that have protective effects in ALD patients and in further elucidating the mechanisms of their actions.

• Journal of Microbiology and Biotechnology
• /
• 제13권3호
• /
• pp.444-450
• /
• 2003

In order to develop an effective means to treat P. aeruginosa infections, we have purified P. aeruginosa outer membrane proteins (OMPs)-specific human IgG antibody. In this study, we investigated the protective activity of the purified anti-OMPs IgC against P. aeruginosa infection in a rabbit endophthalmitis model. Rabbits were inoculated by an intravitreal injection with P. aeruginosa, and treated with a single dose of 1 mg anti-P. aeruginosa OMPs IgG. All the control rabbits predominantly developed edematous responses and opacity in the eyes, but the rabbits treated with the antibody showed only very limited degree of edema. Aliquots of the vitreous humor were extracted and analyzed for the number of viable bacteria and endotoxin level. The results showed that the anti-OMPs IgC significantly reduced the bacterial count compared with the control group, and that the endotoxin level of the vitreous from the IgG-treated rabbits was more than 70-fold lower 6 h after the administration than the control animals. These data suggested that the anti-P. aeruginosa OMPs IgG is effective in inhibiting the bacterial growth and thereby in reducing endotoxin levels in the vitreous, warranting further development of the anti-P. aeruginosa OMPs IgG as a therapeutic means for treating Pseudomonas endophthalmitis.

• Journal of Microbiology and Biotechnology
• /
• 제14권3호
• /
• pp.525-531
• /
• 2004

A strain antagonistic to Fusarium solani, CHO104, was selected from approximately 100 microorganisms isolated from soil. Strain CHO104 was identified as Bacillus amyloliquefaciens and found to be Gram-positive based on the Biolog system and 16S rRNA methods. A culture broth of B. amyloliquefaciens CHO104 also exhibited antimicrobial activity against various microorganisms. As such, the EtOAc extract of the culture broth was isolated by various column chromatographic procedures and HPLC. The antimicrobial and antifungal substance was then characterized as macrolactin A $(C_{24}H_{34}O_5)$ using high-resolution EI-MS and NMR analyses, and found to be very effective in inhibiting the growth of Staphylococcus aureus, E. coli, and Botrytis cinerea, even when using a concentration of one-twentieth of the benzoic acid as the control compound.

• 한국미생물·생명공학회지
• /
• 제31권2호
• /
• pp.135-139
• /
• 2003

Melanin 생합성에 key enzyme인 tyrosinase 억제가 melanin색소 억제에 주된 요인으로 알려져 있지만, 생합성기작에는 여러 다른 요인들이 작용하기 때문에 일차적인 탐색 단계에서 tyrosinase 저해 활성뿐만 아니라 S. bikiniensis의 melanin생합성 억제 연구를 병행하여 천연물을 탐색하였다. 그 중 선택된 궁궁이 (Angelica poiymorpha MAXIM)에서 3가지 물질을 분리하였으며, 3가지 물질 모두가 100$\mu\textrm{g}$/$m\ell$ 이상 농도에서도 tyrosinase억제 활성이 나타나지 않는 반면, S. bikiniensis melanin 생합성 저해 효과를 보여 주었다. 동일 농도에서 S. bikinieffsis의 생장에는 영향을 미치지 않았다.

• Natural Product Sciences
• /
• 제18권4호
• /
• pp.244-249
• /
• 2012

Angelica acutiloba is one of the most intensively cultivated medicinal plants in Korea. The roots of this plant have been used as an important herbal drug, especially for the treatment of various female disorders, as the traditional therapy in Korea and other Asian countries. Consumption of its fresh leaves as a healthy vegetable has recently increased. In this study, essential oil fractions were extracted from the roots and leaves of this plant by steam distillation. Compositions of the two oils were compared by gas chromatography-mass spectrometry (GC-MS). The antibacterial activities of the essential oil were determined against three strains of Escherichia coli. DPPH radical scavenging and reducing power tests were performed to evaluateits antioxidant activities. The cytotoxic activities of the essential oil against a human breast and a uterine cancer cell line were estimated by MTT tests. Additionally, the morphological changes after treatment of the oil fraction were observed under a microscope. The essential oil fraction and its main components, Z-ligustilide and butylidene phthalide, inhibited the growth of three E. coli strains examined, with minimum inhibiting concentrations (MICs) ranging from 1.0 mg/ml to 8.0 mg/ml. Additionally, the essential oil fraction of A. acutiloba exhibited significant DPPH free radical scavenging activity and reducing power. Significant cytotoxic activities of the A. acutiloba essential oil were observed for human uterine (Hela) and breast (MCF-7) cancer cell lines.