• Title/Summary/Keyword: growth simulation

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Simulation of Dynamic Behavior of Glucose- and Tryptophan-Grown Escherichia coli Using Constraint-Based Metabolic Models with a Hierarchical Regulatory Network

  • Lee Sung-Gun;Kim Yu-Jin;Han Sang-Il;Oh You-Kwan;Park Sung-Hoon;Kim Young-Han;Hwang Kyu-Suk
    • Journal of Microbiology and Biotechnology
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    • v.16 no.6
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    • pp.993-998
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    • 2006
  • We earlier suggested a hierarchical regulatory network using defined modeling symbols and weights in order to improve the flux balance analysis (FBA) with regulatory events that were represented by if-then rules and Boolean logic. In the present study, the simulation results of the models, which were developed and improved from the previou model by incorporating a hierarchical regulatory network into the FBA, were compared with the experimental outcome of an aerobic batch growth of E. coli on glucose and tryptophan. From the experimental result, a diauxic growth curve was observed, reflecting growth resumption, when tryptophan was used as an alternativee after the supply of glucose was exhausted. The model parameters, the initial concentration of substrates (0.92 mM glucose and 1 mM tryptophan), cell density (0.0086 g biomass/1), the maximal uptake rates of substrates (5.4 mmol glucose/g DCW h and 1.32 mmol tryptophan/g DCW h), and lag time (0.32 h) were derived from the experimental data for more accurate prediction. The simulation results agreed with the experimental outcome of the temporal profiles of cell density and glucose, and tryptophan concentrations.

Analysis of Weladbility on Resistance Spot Weld for 3 Steel Sheets of Automotive Car Body Using Simulation Method and Nugget Growth Curve (시뮬레이션 및 너겟 성장 곡선을 이용한 자동차 차체용 3겹 강판의 저항점 용접성 분석)

  • Park, Young-Whan
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.11 no.9
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    • pp.3155-3160
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    • 2010
  • Nowadays, most car manufactures have tried to improve fuel efficiency and corrosion resistance of car body. Therefore, use of high strength steels and coated steel becomes more and more increased. In this study, spot weld characteristics according to lap sequence of sheets were analyzed using simulation method for three different steel sheet of car body which were EDDQ class coated steel with 0.7t, high strength steel 440R with 1.2t and advanced high strength steel DP 590 with 1.0t. Using simulation, weldability was evaluated by nugget size of welded zone according to nugget growth curve and welding current with respect to lap sequence of sheets. Contact resistance of each sheets contact point was used to analyze formation of nugget and optimal lap sequence was suggested.

Possibility of Climate Change and Simulation of Soil Moisture Content on Mt. Hallasan National Park, Chejudo Island, Korea

  • Kim, Eun-Shik;Kim, Young-Sun
    • The Korean Journal of Ecology
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    • v.23 no.2
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    • pp.117-123
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    • 2000
  • Changing patterns and the possibility of climate change in the area of Cheiudo island, the southernmost Island in Korea, were analyzed using daily temperature and Precipitation data observed at the Cheiu Regional Meteorological Office from May 1923 to December 1998. A hydrologic simulation model "BROOK" was used to simulate and analyze the dynamics of daily soil moisture content and soil moisture deficit by applying the daily weather data. During the period, significantly increasing pattern was observed in temperature data of both annual and monthly basis, while no significantly changing pattern was observed in precipitation data. During the last 76 years. mean annual temperature was observed to have risen about 1.4$^{\circ}C$, which may show the Possibility of the initiation of climate change on the island whose validity should be tested in future studies after long-term studies on temperature. Based on the simulation, due to increased temperature, significant increase was predicted in evapotranspiration. while no significant decrease was detected in simulated soil moisture content during the period. Changing pattern of annual soil moisture content was markedly different from those of precipitation. In some dominant trees, negative effects of the drought of the late season for the previous year were shown to be statistically significant to radial growth of the tree for the current year. As annual variation of radial growth of trees is mainly affected by the soil moisture content. the information on the dynamics of soil moisture deficit possibly provides us with useful information for the interpretation of tree growth decline on the mountain. mountain.

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PWSCC Growth Assessment Model Considering Stress Triaxiality Factor for Primary Alloy 600 Components

  • Kim, Jong-Sung;Kim, Ji-Soo;Jeon, Jun-Young;Kim, Yun-Jae
    • Nuclear Engineering and Technology
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    • v.48 no.4
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    • pp.1036-1046
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    • 2016
  • We propose a primary water stress corrosion cracking (PWSCC) initiation model of Alloy 600 that considers the stress triaxiality factor to apply to finite element analysis. We investigated the correlation between stress triaxiality effects and PWSCC growth behavior in cold-worked Alloy 600 stream generator tubes, and identified an additional stress triaxiality factor that can be added to Garud's PWSCC initiation model. By applying the proposed PWSCC initiation model considering the stress triaxiality factor, PWSCC growth simulations based on the macroscopic phenomenological damage mechanics approach were carried out on the PWSCC growth tests of various cold-worked Alloy 600 steam generator tubes and compact tension specimens. As a result, PWSCC growth behavior results from the finite element prediction are in good agreement with the experimental results.

Analysis of Hygrothermal Performance of Wood Frame Walls according to Position of Insulation and Climate Conditions

  • Kang, Yujin;Chang, Seong Jin;Kim, Sumin
    • Journal of the Korean Wood Science and Technology
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    • v.44 no.2
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    • pp.264-273
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    • 2016
  • The insulation of a building envelope influences the hygrothermal performance as well as the thermal performance of the building. While most of Korean wood frame houses have an interior insulation system, the exterior insulation system with high thermal performance has recently been applied. While it can be effective in energy savings for better insulation performance, without consideration of the moisture, condensation and mould growth can occur. Therefore, in this study, hygrothermal behaviour, water content, and mould growth were analyzed using hygrothermal simulation of an exterior wall of a wood frame house with which the interior insulation and exterior insulation systems were applied. The wall layer included Wall A (Interior insulation) and Wall B (Exterior insulation). The U-values were identified as 0.173 and $0.157W/m^2K$, respectively. The total water content and OSB absolute water content of Wall A were confirmed to be higher than those of Wall B, but the absolute water content did not exceed the reference value of 20%. The moisture content of the two walls was determined to be stable in the selected areas. However, mould growth risk analysis confirmed that both Wall A and Wall B were at risk of mould growth. It was confirmed that as the indoor setting temperature decreased, the mould index and growth rate in the same area increased. Therefore, the mould growth risk was affected more by indoor and outdoor climate conditions than by the position of the insulation. Consequently, the thermal performance of Wall B was superior to that of Wall A but the hygrothermal performances were confirmed to be similar.

Large-Scale Integrated Network System Simulation with DEVS-Suite

  • Zengin, Ahmet
    • KSII Transactions on Internet and Information Systems (TIIS)
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    • v.4 no.4
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    • pp.452-474
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    • 2010
  • Formidable growth of Internet technologies has revealed challenging issues about its scale and performance evaluation. Modeling and simulation play a central role in the evaluation of the behavior and performance of the large-scale network systems. Large numbers of nodes affect simulation performance, simulation execution time and scalability in a weighty manner. Most of the existing simulators have numerous problems such as size, lack of system theoretic approach and complexity of modeled network. In this work, a scalable discrete-event modeling approach is described for studying networks' scalability and performance traits. Key fundamental attributes of Internet and its protocols are incorporated into a set of simulation models developed using the Discrete Event System Specification (DEVS) approach. Large-scale network models are simulated and evaluated to show the benefits of the developed network models and approaches.

Evaluation of Properties of Sulfur-Oxidizing Bacteria Growth and Resistance to Biochemical Corrosion by Simulation Test (시뮬레이션 시험에 의한 황산화세균의 생장 특성 및 생화학적 부식 저항성 평가)

  • Kim, Gyu-Yong;Lee, Eui-Bae;Khil, Bae-Su;Lee, Seung-Hun
    • Journal of the Korea Concrete Institute
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    • v.20 no.4
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    • pp.495-502
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    • 2008
  • To analyze the growth of SOB(Thiobacillus novellus) and biochemical corrosion of concrete, simulation test method and device were developed, and basic conditions for SOB growth were established. Two types of simulation tests were conducted according to a transplant method and a concentration of $H_2SO_4$. As a result, the SOB growth in distinct manners and antibiosis of specimen were observed. In the case of the specimens indirectly transplanted with SOB through culture solution submersion at a hydrogen sulfide level of 120 ppm, the rapid activation of SOB and the resulting sulfuric acid production were observed. However, SOB were shown to grow rapidly and then die out in a relative short period of time. Meanwhile, in the case of the specimens directly transplanted with SOB at a hydrogen sulfide level of 50 ppm, the long-term growth of SOB was possible, but the production of sulfuric acid by SOB did not progress. In the case of the antibiotic metal-mixed specimens, SOB with destroyed cell membranes and internal organizations were observed.