• Journal of The Korean Society of Grassland and Forage Science
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• v.17 no.4
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• pp.345-350
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• 1997

Using modified Lincoln solution, hydroponic system was developed to compare simultaneously a range of pasture species in terms of relative growth rate(RGR), rate of roots to shoots, and relative root volume and root area. Modified Lincoln solution achieved optimum growth of nineteen forage species tested, and was proved to be a good method for studying plant root characteristics. RGR was measured sucesshlly showing reasonably consistant values over time. There was a much differences in rootkhoot ratio between species within groups as between groups. Root tissue density was lower in grasses than legume or herb group. Root area index was very high for grasses and herbs, and this index should be useful for evaluating the comparative root system of different species.

• Korean Journal of Plant Tissue Culture
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• v.27 no.4
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• pp.309-315
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• 2000

Panax ginseng is an important medicinal plant that has been used worldwide for geriatric, tonic, stomachic, and aphrodisiac treatments. Ginsenosides contained in the ginseng root are the main substances having active functions for human body. The price of ginseng is very expensive due to a complex process of cultivation, and the yield of ginseng is limited, which cannot meet the demand of the increasing market. Researchers have applied plant biotechnology to solve the problems but there are still things to be determined towards ginsenoside production by large-scale adventitious root culture. In this experiment, 5 to 20 liter bioreactors were employed to determine optimal conditions for adventitious root culture and ginsenoside production of Panax gineng. Callus was induced from the ginseng root on MS agar medium containing 1.0 mg. $L^{-1}$ 2,4-D and 0.1 mg. $L^{-1}$ kinetin. Then the callus was cultured on MS agar medium supplemented with 2.0 mg. $L^{-1}$ IBA, 0.1 mg. $L^{-1}$ kinetin, and 30 g. $L^{-1}$ to induce adventitious roots. The maximum root growth and ginsenoside production were obtained in 1/2 MS medium. 2.0 mg. $L^{-1}$ naphthalene acetic acid resulted in greater root growth than 2.0 mg $L^{-1}$ indole-3-butyric acid. Ginsenoside content increased with 2.0 mg. $L^{-1}$ benzyl adenin or kinetin. High concentrations of benzyl adenin (above 3.0 mg. $L^{-1}$ ) decreased the adventitious root growth and ginsenoside productivity. N $H_{4}$$^{+}$ inhibited the ginsenoside accumulation, while high concentrations of $K^{+}$, $Mg_{2}$$^{+}$, and $Ca_{2}$$^{+}$ increased it. N $H_{4}$$^{+}$ at 0.5 and 1.0 times of the normal amount in 3/4 SH medium resulted in the greatest biomass increase, but the highest ginsenoside productivity was obtained when N $O_{3}$$^{-}$ was used as the sole nitrogen source in the medium. Most microelements at high concentrations in the medium inhibited the root growth, but high concentrations of MnS $O_4$enhanced the root growth. Root dry weight increased with increasing sucrose concentrations up to 50 g. $L^{-1}$ , but decreased from 70 g $L^{-1}$ Ginsenoside productivity was maximized at the range of 20 to 30 g. $L^{-1}$ sucrose. In the experiment on bioreactor types, cone and balloon types were determined to be favorable for both adventitious root growth and ginsenoside production. Jasmonic acid was effective for increasing ginsenoside contents and Rb group ginsenosides mainly increased. These results could be employed in commercial scale bioreactor cultures of Panax ginseng.x ginseng.

• Korean Journal of Soil Science and Fertilizer
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• v.3 no.1
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• pp.43-48
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• 1970

In order to investigate the inter-relation with the growth of the barley root and GOT and GPT activities the growth of root and GOT, GPT activities were measured those which have been supplied various nitrogen compounds ($NO_3-N$, $NH_4-N$, Urea, and Amino acid). The results obtained are summarized as follows: 1. Growth of barley root supplied with $NH_4-N$ is generally increased in length and weight compared with that of the root fertilized by $NH_4-N$. 2. The above-mentioned root with $NH_4-N$ is not only decreased in its weight and length but also is apt to inhibited its growth, in compared with the root provided with $NO_3-N$. 3. The activities of GOT and GPT for the root fertilized by $NH_4-N$, the badly grown root is generally increased, while of the root supplied with $NO_3-N$ is decreased compared with that of the root fertilized by $NH_4-N$. 4. The activities of GOT and GPT for the root provided with amino acid known as the considerable growth inhibiting compound for rice is generally decreased, while that of the badly known-grown root is increased. 5. The activities of GOT and GPT in the supernatant fraction of the barley is for the most part, high and low in the mitocondrial fraction.

• Korean Journal of Organic Agriculture
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• v.6 no.1
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• pp.151-160
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• 1997

Azospirillum sp., photosynthetic bacteria(Rhodopseudomonas sp.) and Pseudomonas sp. were separated and screened from soil and soilless culture, and identificated. The antifungal activities against root-infected pathogens and plant growth promoting effects of the cultured solution of the starins(5.0$\times$105 cells/$m\ell$) in the peatmoss compost on the early growth of cucumber and tomato seedling were investigated. Azospirillum sp. and Pseudomonas sp. showed a antifungal activities against Fusarium sp., Pythium sp. and Rhizoctonia sp in thed ranges of 51.0% to 72.0% on potato dextrose agar medium, however photosynthetic bacteria had not antifungal activities. When cultured solution of Azospirillum sp., photosynthetic bacterial and Pseudomonas sp. were bacterialized by mixing with peatmoss compost, early growth of cucumber and tomato in terms of plant height, number of leaves, leaf area, root length, fresh anf dry weight of leaf, stem and root were promoted, especially photosynthetic bacteria had a the best plant growth promting activities.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.45 no.5
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• pp.310-315
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• 2000

Alfalfa (Medicago sativa L.) plants have been reported to contain water-soluble substances that are autotoxic as well as allelopathic. Laboratory experiment through a petri-dish assay with imbibed seeds was conducted to evaluate both autotoxic and allelopathic effects of alfalfa leaf extracts on the germination and early seedling growth of alfalfa, red clover, crested wheatgrass, and Russian wildrye. Alfalfa seed germination was delayed dependent on extract concentration, with no difference in final germination at 72 hours. Root growth of alfalfa was stimulated up to 14% above control at very low concentrations of both leaf and stem extracts of alfalfa and was significantly reduced at extract concentration of more than 0.5g dry tissue/L (${gL}_{-1}$). Leaf extracts were generally more autotoxic for root growth than were stem extracts. Hypocotyl growth was not affected by all the concentrations of both leaf and stem extracts. Root length of legumes was more sensitive to the autotoxic chemicals from leaf extracts than was germination or shoot length. Hypocotyl growth of two legume plants and plant height of two grasses were not influenced by extracts. Seed germination and root growth of legumes were more inhibited by aqueous extracts of alfalfa leaf than were those of grasses. This result indicates autotoxic effect of alfalfa leaf extracts seems to be greater than allelopathic effect.

• Journal of The Korean Society of Grassland and Forage Science
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• v.12 no.1
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• pp.6-11
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• 1992

This experiment was carried out to study the biological yields(BY) of varieties by the developmental growth stages and shoot weight(SHW), leaf area(LA), root dry weight(RW), number of tillers(NT), shoot and root ratio(S/R), weight of a tiller(WT) as factors of yield components with 5 varieties of perennial ryegrass. The results are summarized as follows; 1. The biological yields in summer were about 115 of spring yield. 2. The biological yields of Manhattan were the highest until1 third developmental growth stage among perennial ryegrasses, and those for Maprima and P-2 varieties were the highest in the following stages. In terms of shoot dry weight, the similarly tendency appeared in the fifth developmental growth stage. 3. The shoot dry weight increased highly with the root dry weight. 4. The Tempo variety of biological yield was generally low but the weight of a tiller was the highest. 5. The highest increasing stage of biological yields was between fifth and sixth developmental growth stage, but the lowest increasing stage of biological yields was between first and second developmental growth stage, respectively. The dominant factors of biological yields were influenced to shoot and root dry weight.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2020.12a
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• pp.59-59
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• 2020

Salix gracilistyla has a characteristic of fast growth especially in the river side or infertile soil, showing a lot of lateral branches and a strong root system among the Salix. spp. On the basis of results observed, we provide several growth characteristics of S. gracilistyla among some selected provenances. In two-year results, root collar diameter ranged from 33.5 mm to 56.7 mm. Taeback showed the lowest value, and Jeongseon and Hoengseong were higher than the other areas in the traits above. Height growth was the lowest in Samcheok and the highest in Wonju. To examine growth and development of lateral branches is important because S. gracilistyla has a lot of lateral branches which account for high biomass production. Length of lateral branch showed high variation, in which the highest number was observed in Yeongwol and the lowest in Samcheok. Many lateral branches were shown in Wonju. Preliminary study on biomass resources evaluation, we analyzed correlation between volume and length, and volume and root diameter. High correlation was observed between root diameter and plant volume. And we compared the observed volume with the caculated volume by regression formula. To evaluate Salix species as biomass resources, it is needed to survey growth characteristics continuously and adopt selection of individuals or families of high biomass and caloric values by reliable data. In addition, Salix spp. can be also applicable to selection breeding regime because of feasible propagation and fast growth.

• Journal of Sericultural and Entomological Science
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• v.20 no.1
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• pp.1-4
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• 1978

The root-out of mulberry saplings has been studied by pour into soil and spray on leaves of the plant growth substances. In addition, the effect of "Rutin", a kind of plant growth hormones has been also studied on promoting the rootability of mulberry scions by dipping the scions at the various concentrations. The results are summarized as follows: (1) It is likely to be effective on acceleration of the rootability resulting in increase of rooting ratio by pour into soil at the concentration of 1 ppm and 10 ppm NAA, 10 ppm and 100 ppm Rutin, and 5000 fold solution of Atonic, respectively (2) Growth of branch is only promoted by leaf spray of 0.5% urea, 0.005% and 0.01% Rutin, and 5000 fold solution of Atonic without increasing the root weight. (3) It seems that 0.05% of Rutin is of practical use, and 0.05% to 0.4% of "Rutin" accelerates the root-out of mulberry scions as well as NAA does.

• The Korean Journal of Ecology
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• v.23 no.4
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• pp.323-329
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• 2000

The allelopathic effects of volatile substances from Chamaecyparis obtusa (S. et Z.) Endl. were examined on the germination and seedling growth of some plant species, and on the population growth of some microorganisms. The germination and seedling growth of the receptor plants were suppressed more severely by leaf and fruit essential oils than by those of other parts. Colonial growth of fungi was severely inhibited by essential oils extracted from leaves and fruits. The development of root hairs of the receptor plants was also severely inhibited by the essential oils. The cortical cells at the root tips of Lactuca sativa L. treated with essential oils showed contraction of the cytoplasm, resulting in plasma membranes becoming detached from the cell walls and the cells metamorphosing irregularly. Accumulation of lipid granules inside the contracted cytoplasm and degeneration of mitochondrial cristae were also observed.

• Korean Journal of Plant Resources
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• v.11 no.3
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• pp.353-357
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• 1998

This study was conducted to determine the effect of growth regulators, genotype, and cutting position on the rooting and root growth from cutting of Chrysanthemum zawadskii H.. Rooting rate of Keungugeolcho in the treatement of IBA 500 and 1000 ppm was the better than those of other treatments of IAA, NAA and Rooton. Rooting rate differed depending on the genotype. Hangryobonggugeolcho was better than Keungucheolcho in rooting rate. The treatment of rooton remarkably induced many roots from the cuttings of eight accessions of Chrysanthemum zawadskii H.. Also, rooting rate and number of root differed depending on cutting position. When cuttings including shoot tip were cultured on tray containing bed soil, rooting rate and number of root induced from cuttings with shoot tip was higher than when cuttings without shoot tip and with lateral axillary bud were cultured.