• Title/Summary/Keyword: growth and activities

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Effect of High Dietary Carbohydrate on the Growth Performance, Blood Chemistry, Hepatic Enzyme Activities and Growth Hormone Gene Expression of Wuchang Bream (Megalobrama amblycephala) at Two Temperatures

  • Zhou, Chuanpeng;Ge, Xianping;Liu, Bo;Xie, Jun;Chen, Ruli;Ren, Mingchun
    • Asian-Australasian Journal of Animal Sciences
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    • v.28 no.2
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    • pp.207-214
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    • 2015
  • The effects of high carbohydrate diet on growth, serum physiological response, and hepatic heat shock protein 70 expression in Wuchang bream were determined at $25^{\circ}C$ and $30^{\circ}C$. At each temperature, the fish fed the control diet (31% CHO) had significantly higher weight gain, specific growth rate, protein efficiency ratio and hepatic glucose-6-phosphatase activities, lower feed conversion ratio and hepatosomatic index (HSI), whole crude lipid, serum glucose, hepatic glucokinase (GK) activity than those fed the high-carbohydrate diet (47% CHO) (p<0.05). The fish reared at $25^{\circ}C$ had significantly higher whole body crude protein and ash, serum cholesterol and triglyceride, hepatic G-6-Pase activity, lower glycogen content and relative levels of hepatic growth hormone (GH) gene expression than those reared at $30^{\circ}C$ (p<0.05). Significant interaction between temperature and diet was found for HSI, condition factor, hepatic GK activity and the relative levels of hepatic GH gene expression (p<0.05).

Studies on the Catalase Activities of Tomato (Lycopersicum esculentum) as a Function of Age (토마토의 성장과정에 따른 카탈리아제 (Catalase) 활성에 관한 연구)

  • Sie Won Park;Duk Shim Kim
    • Journal of the Korean Chemical Society
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    • v.37 no.12
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    • pp.1068-1075
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    • 1993
  • Catalase activities were assayed on the leaves and roots of Tomato (Lycopersicum esculentum) as a function of age for 1 year after germination. The enzyme activities of root tissue demonstrated to be insignificant through all stages of development. On the other hand, the catalase activities of leaf peroxisomal fractions showed remarkable changes with peak value of 76 ${\mu}mol$/ml/min at germination stage within 2 weeks growth, 7.2 ${\mu}mol$/ml/min at adult stage around 4∼5 months growth and very small activities at aged period around 11∼12 months growth. It is suggested that there may be two factors concerning such catalase activities in relation with age, firstly the glyoxisomal reactions including glyoxylate cycle and $\beta$-oxidation at germination stage and secondly photosynthesis hereafter seemed to affect age dependent changes of catalase activity by producing coincident amount of $H_2O_2$. In addition, NADPH coenzyme was found to have ability to restore catalytic acitivity of inactivated catalase (compound II) at all stages of development except old age, indicating NADPH would play a role as catalase protector against deleterious substrate,$H_2O_2$.

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Effects of Gamma Radiation on the Germination, Growth and Enzyme (peroxidase and catalase) Activities of Old Vegetable Seed (묵은 채소 종자의 발아와 생육 및 효소활성에 미치는 $\gamma$선의 영향)

  • 김재성;백명화;김동희;이영근;정규회
    • Korean Journal of Environmental Biology
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    • v.19 no.3
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    • pp.205-210
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    • 2001
  • To determine the effect of low dose gamma radiation on the germination and enzyme activities, seeds of Chinese cabbage (Brassica compestris L. cv. Hanyoreum) and radish (Raphanus sativus L. cv. Chungsukoungzoung) were irradiated at the dose of 2-50 Gy. The germination rate of irradiation group was higher than that of the control. Especially it was highest at the early stage. The germination rate of Chinese cabbage was high at 2 Gy and 8 Gy irradiation group and that of radish was high at 2 Gy, 6 Gy and 10 Gy irradiation group. Growth of both seedlings of Chinese cabbage and radish increased positively in low dose irradiation group. The height of Chinese cabbage was noticeably high at 4 Gy and 10 Gy irradiation group and that of radish at 6 Gy irradiation group. The protein contents of seedlings from seeds irradiated with the low dose gamma radiation was higher than the control, especially at the early stage. The enzyme activities of seedlings from seeds irradiated with the low dose gamma radiation was high at 4 Gy and 10 Gy irradiation group. These results suggest that the germination, growth and enzyme activities of old vegetable seeds could be promoted by the low dose gamma radiation.

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Biological Activities of Essential Oils from Angelica tenuissima Nakai

  • Roh, Junghyun;Shin, Seungwon
    • Natural Product Sciences
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    • v.19 no.4
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    • pp.297-302
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    • 2013
  • The current study was conducted to evaluate the antibacterial and antioxidant activities of the essential oil fraction from the roots of Angelica tenuissima Nakai and its main components. We extracted the essential oil fraction from the roots of A. tenuissima using steam distillation and isolated its main components. Their antibacterial activities were determined by broth dilution test against food-borne pathogenic bacteria. Antioxidant activities were evaluated by DPPH-scavenging assay and reducing-power test. Also tested was their ability to inhibit the growth of two gastrointestinal cancer cell lines, Caco-2 and MKN-45. The A. tenuissima oil fraction and its main components, ligustilide and butylidene phthalide exhibited marked inhibitory effects against most of the tested antibiotic-susceptible and antibiotic-resistant bacterial strains with minimum inhibiting concentrations (MICs) from $0.21{\pm}0.08$ to $3.60{\pm}0.89mg/ml$. They also showed growth-inhibiting activity against Caco-2 and MKN-45 cells. The oil fraction showed significant antioxidant activities in DPPH radical scavenging assay and reducing-power test. Taken together, A. tenuissima essential oil could be used as a safe additive for preventing food contamination by pathogenic bacteria. Additionally, its antioxidative activity and the ability to inhibit gastrointestinal carcinoma cell lines could increase its value for functional foods and prevention of cancer.

Antifungal Activities on Organic Heritage Fungi and Antioxidative effect of Phellodendron amurense Extractives (황벽나무 추출물의 유기질 문화재 오염균에 대한 항균성 및 항산화 활성)

  • Hong, Jin-Young;Kim, Young-Hee;Jung, Mi-Hwa;Jo, Chang-Wook;Choi, Jung-Eun
    • Journal of Korea Technical Association of The Pulp and Paper Industry
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    • v.42 no.5
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    • pp.54-61
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    • 2010
  • Antifungal and antioxidative activities of Phellodendron amurense extracts were investigated for use as a natural preservative. After separation of P. amurense into phloem, xylem, leaf and fruit each part was subjected to methanol extraction. Each MeOH extract was further fractionated with several solvents(n-hexane, methylene chloride and ethylacetate). Among the methanol extracts, extracts of phloem and leaf inhibited effectively the growth of mold fungi and rot fungi, respectively. Especially, ethylacetate fraction from phloem showed the highest growth inhibitory effects against fungi tested, such as P. citreonigrum H3, P. toxicarium H4, P. corylophilu H5, A. clavatus, P. osteatus, S. commune, and G. lucidum. The fractions of fruit, which had lower antifungal activities mostly than those of phloem, strongly inhibited rot fungi such as G. lucidum, T. versicolor, and T. palustris. Compared to ferulic acid which is well known antioxidant, ethylacetate fraction of fruit showed high antioxidative activities on concentration of 1 to $100{\mu}g/m{\ell}$ in DPPH radical scavenging activity. As a result, antifungal and antioxidative activities of P. amurense suggest that its extract and fraction have a possibility as conservative of cultural heritage because it might get conservation effects against deteriorating microorganisms of cultural heritage.

Antifungal activities of sulphamide and dicarboximide fungicides against Botrytis cinerea in several in vitro bioassays (여러 종류의 in vitro 생물검정에서 Botrytis cinerea에 대한 sulphamide계와 dicarboximide계 살균제의 활성 특성)

  • Choi, Gyung-Ja;Kim, Heung-Tae;Kim, Jin-Cheol;Cho, Kwang-Yun
    • The Korean Journal of Pesticide Science
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    • v.3 no.3
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    • pp.37-44
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    • 1999
  • Two sulphamide (dichlofluanid and tolylfluanid) and three dicarboximide fungicides (iprodione, vinclozolin, procymidone) were used to investigate the correlation between in vitro antifungal activities and in vivo disease controlling activities against Botrytis cinerea, a causal agent of tomato gray mold and to develop efficient in vitro assays. They controlled effectively the development of tomato gray mold disease in vivo and their controlling activities were similar one another. However, several in vitro assays revealed that their in vitro antifungal activities were quite different between sulphamide and dicarboximide fungicides; the formers showed stronger inhibition activities for spore germination than the latters, whereas the formers inhibited mycelial growth less severely than the latters. The results indicate that the fungicides having different modes of action can show different in vitro antifungal activities according to in vitro assays, even if they have similar in vivo disease controlling activities. On the other hand, two rapid and efficient in vitro assays named Microtiter plate methods I (MPM I) and II (MPM II) were developed for the evaluation of fungicides for inhibitory activities against spore germination and mycelial growth of B. cinerea, respectively. The antifungal activities of five fungicides of two chemical groups in MPM I and II were correlated with the inhibitory activities against spore germination and mycelial growth using solid media, respectively.

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Effect of human milk on the angiogenesis and endothelial cell growth (혈관현성 및 혈관내피세포의 성장에 미치는 모유의 영향)

  • Lee, Ok-Hee;Kim, Jung-Sun;Shim, Kyung-Hee;Kim, Kyu-Won;Chung, Hae-Young
    • Journal of Life Science
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    • v.5 no.3
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    • pp.117-120
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    • 1995
  • Human milk was examined for antiangiogenic activities by using the chick embryo chorioallantoic membrane (CAM) assay and endothelial cell growth. The low molecular weight (20 KD>$\sim$ ) fraction of human milk stimulated the angiogenesis and increased the endothelial cell growth. These results suggest that the increase of angiogenesis and endothelial cell growth might be attributed to several growth factors and/or angiogenic factors in low molecular fraction (20 KD>$\sim$) of human milk.

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Effects of Various Nitrogen Compounds for the Growth of Barley Roots and Transaminase Activity (대맥근(大麥根)의 생장(生長)과 Transaminase의 활성(活性)에 미치는 몇 가지 질소화합물(窒素化合物)의 영향(影響))

  • Kim, K.S.
    • Korean Journal of Soil Science and Fertilizer
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    • v.3 no.1
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    • pp.43-48
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    • 1970
  • In order to investigate the inter-relation with the growth of the barley root and GOT and GPT activities the growth of root and GOT, GPT activities were measured those which have been supplied various nitrogen compounds ($NO_3-N$, $NH_4-N$, Urea, and Amino acid). The results obtained are summarized as follows: 1. Growth of barley root supplied with $NH_4-N$ is generally increased in length and weight compared with that of the root fertilized by $NH_4-N$. 2. The above-mentioned root with $NH_4-N$ is not only decreased in its weight and length but also is apt to inhibited its growth, in compared with the root provided with $NO_3-N$. 3. The activities of GOT and GPT for the root fertilized by $NH_4-N$, the badly grown root is generally increased, while of the root supplied with $NO_3-N$ is decreased compared with that of the root fertilized by $NH_4-N$. 4. The activities of GOT and GPT for the root provided with amino acid known as the considerable growth inhibiting compound for rice is generally decreased, while that of the badly known-grown root is increased. 5. The activities of GOT and GPT in the supernatant fraction of the barley is for the most part, high and low in the mitocondrial fraction.

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Characteristics of Photosynthetic Electron Transport Activity in Isolated Chloroplast of Korean Ginseng and Radish (인삼과 무 엽록체의 광합성 전자전달 활성)

  • 김갑식
    • Journal of Plant Biology
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    • v.33 no.2
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    • pp.111-118
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    • 1990
  • In order to characterize the chloroplasts of Korean ginseng as a semi-shade plant and radish as a sun plant, effects of growth light intensity on photosynthetic electron transport (PS) activity in chloroplasts and superoxide (O2.-) production in thylakoid membrane by irradiation were investigated. High-light chloroplasts of both plants showed higher PS activities than those grown under ow growth light intensity. High PS II and low PS I activities in ginseng chloroplasts (ratio of PS II/PS I : 1.1) were observed, but radish chloroplasts showed low PS II and high PS I activities (ratio of PS II/PS I : 0.3). PS II activity of both plants was little affected by temperature in range of 15-35$^{\circ}C$. Activities of whole -chain (PS II+I) in ginseng and PS I in radish were increased at high temperature (4$0^{\circ}C$). Preincubation of chloroplasts at 4$0^{\circ}C$ during 30 min, as a mild heat stress, caused rapid decrease in PS II and PS II+I activities of both plants. However PS I activity was not decreased in ginseng and rather increased in radish. O2.- production (NBT reduction) in Mehler reaction in the thylakoid membrane was inhibited by DCMU in both plants. DMBIB inhibited O2.- production in ginseng, but radish was insensitive to DMBIB. Electron flow system in ginseng thylakoid membrane was more susceptible to damage of photooxidation than that of radish.

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R&D Investment Effect through Patent on IT firms using Panel Structural Equations (패널구조방정식을 활용한 IT기업의 R&D투자효과 연구: 특허 매개효과 중심으로)

  • Lee, Jongho;Kim, Tae Hwan;Jung, Woo-Jin
    • Knowledge Management Research
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    • v.21 no.1
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    • pp.137-150
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    • 2020
  • This study analyzes not only the direct impact of R&D investment on corporate growth for 578 private firms in 2007-2016, but also whether corporate innovation activities play a role as a mediating parameter between R&D investment and corporate growth. For this purpose, we classify companies into IT and non-IT companies and measure the mediating effect by dividing innovation activities into the number of registered patents, applied patents, and sum of them. In addition, this study is based on both the systemGMM which is considered to be effective in solving the endogenous problems caused by the cross-sectional analysis in previous studies and ML-SEM which is a new method recently, and then compares two results. According to the empirical results, innovation activities has a role as partly mediating parameter on sales growth in non-IT companies. On the other hands, in IT companies, the increase in R&D investment leads to a decrease in sales of the company, and the increase in innovation activities increases the sales of the company. However, it was confirmed that IT companies also had positive effects by adjusting the lag of the R&D. In other words, this suggests that securing patents is more important than R&D investment for direct sales growth of IT companies. It is also evidence that immediate introduction of technology is necessary to respond to the speed of technological change since the cycle time of technologies of the IT field is relatively shorter compared to that of other fields.