• Title/Summary/Keyword: group detection

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Quantitative Analysis of the Twenty Marker Components in Gwakhyangjeonggi-san using Ultra-Performance Liquid Chromatography with Mass Spectrometer (LC-MS/MS를 이용한 곽향정기산(藿香正氣散) 추출물 중 20종 성분의 함량분석)

  • Seo, Chang-Seob;Shin, Hyeun-Kyoo
    • Korean Journal of Pharmacognosy
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    • v.45 no.2
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    • pp.113-120
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    • 2014
  • Generally, Gwakhyangjeonggi-san has been used for treatment of diarrhea-predominant irritable bowel syndrome. In this study, an ultra-performance liquid chromatography-electrospray ionization-mass spectrometer method was established for the simultaneous quantification of marker compounds 1-20 in Gwakhyangjeonggi-san water extract. All analytes were separated by gradient elution using two mobile phases on a UPLC BEH $C_{18}$ ($100{\times}2.1mm$, $1.7{\mu}m$) column and maintained at $45^{\circ}C$. The injection volume was $2.0{\mu}L$ and the flow rate was 0.3 mL/min with detection at mass spectrometer. Regression equations of the compounds 1-20 were acquired with $r^2$ values ${\geq}0.9950$. The values of limit of detection and quantification of all analytes were 0.01-2.79 ng/mL and 0.03-8.37 ng/mL, respectively. The amounts of the compounds 1-20 in Gwakhyangjeonggi-san water extract were not detected $-3,236.67{\mu}g/g$. The established LC-MS/MS methods will be valuable to improve quality control of traditional herbal formula, Gwakhyangjeonggi-san.

Development of a Single Chain Antibody Using a Phage Display Cloning Method for the Detection of 2,4-Dinitrotoluene

  • Na, Jung-Hyun;Joo, Man-Seok;Lee, Won-Kyu;Shim, Hyunbo;Lim, Si-Hyung;Jung, Sang Taek;Yu, Yeon Gyu
    • Bulletin of the Korean Chemical Society
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    • v.34 no.2
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    • pp.460-464
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    • 2013
  • Single-chain variable fragments of antibodies (scFv) specific to 2,4-dinitrotoluene (DNT) were isolated from a phage library displaying synthetic human scFv fragments with 6 diversified complementary determining regions (CDRs). A DNT derivative that contained an extended amine group was synthesized and conjugated to the NHS-group that was linked to magnetic beads. Phages specific to the immobilized DNT derivatives were isolated from the library after 4 rounds of sequential binding and elution processes. The displayed scFv fragments from the isolated phages showed consensus CDR sequences. One DNT-specific scFv was expressed in E. coli and purified using Ni-affinity chromatography. The purified DNT-specific scFv binds specifically to the immobilized DNT-derivative with $K_D$ value of $6.0{\times}10^{-7}$ M. The scFv and DNT interaction was not disrupted by the addition of 4-nitrotoluene or benzoic acid. These data demonstrate that the screened scFv from the phage displayed library could be used for selective and sensitive detection of explosives such as TNT.

Home Visitation Screening for Child Abuse Assessment in Korea

  • Kim, Hee-Soon;Kim, Tae-Im;Ju, Young-Hee;Lim, Ji-Young;Ha, Young-Ok;Yoo, Ha-Na
    • Child Health Nursing Research
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    • v.18 no.3
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    • pp.95-100
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    • 2012
  • Purpose: The purpose of this study was to facilitate home visits to assess the current rate of child abuse in order to provide an agenda for the early detection and prevention of child abuse and neglect in Korea. Methods: For this retrospective descriptive research, 20 public health centers were selected, 1,991 families were visited and 2,680 children were assessed. Results: We found 415 cases (15.5%) of potential abuse and 7 cases (0.3%) of actual abuse. The greatest risk group was to children age 4 to 6 years. According to the HOME Inventory, there were 17 infants (5.8%) presenting a potential risk for child abuse and neglect. Conclusion: Visitation screening is highly recommended for prevention in the high-risk preschool age group.

A Cluster-based Address Allocation Protocol in MANET Environment (MANET 환경에서 클러스터 기반 주소 할당 프로토콜)

  • Cho, Young-Bok;Lee, Sang-Ho
    • The Journal of Korean Institute of Communications and Information Sciences
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    • v.32 no.9A
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    • pp.898-904
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    • 2007
  • I must receive node discernment address for communication between node that participate to network in MANETs(Mobile Ad-hoc Networks). Address is created by node confidence or different node. I achieve address redundancy check (Duplicate Address Detection) to examine whether this address is available unique address. However, this method happens problem that MANETs' extensity drops. This paper can manage by group unit binding transfer nodes to group in MANETs. I suggest method that apply special quality of cluster that exchange subordinate decrease and mobility government official of control message are easy in address assignment protocol minimize time required in redundancy check and solves extensity problem. Method that propose in this paper shows excellent performance according to node number increase than wave and MANETConf [2] through simulation.

IPv6 Autoconfiguration for Hierarchical MANETs with Efficient Leader Election Algorithm

  • Bouk, Safdar Hussain;Sasase, Iwao
    • Journal of Communications and Networks
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    • v.11 no.3
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    • pp.248-260
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    • 2009
  • To connect a mobile ad hoc network (MANET) with an IP network and to carryout communication, ad hoc network node needs to be configured with unique IP adress. Dynamic host configuration protocol (DHCP) server autoconfigure nodes in wired networks. However, this cannot be applied to ad hoc network without introducing some changes in auto configuration mechanism, due to intrinsic properties (i.e., multi-hop, dynamic, and distributed nature) of the network. In this paper, we propose a scalable autoconfiguration scheme for MANETs with hierarchical topology consisting of leader and member nodes, by considering the global Internet connectivity with minimum overhead. In our proposed scheme, a joining node selects one of the pre-configured nodes for its duplicate address detection (DAD) operation. We reduce overhead and make our scheme scalable by eliminating the broadcast of DAD messages in the network. We also propose the group leader election algorithm, which takes into account the resources, density, and position information of a node to select a new leader. Our simulation results show that our proposed scheme is effective to reduce the overhead and is scalable. Also, it is shown that the proposed scheme provides an efficient method to heal the network after partitioning and merging by enhancing the role of bordering nodes in the group.

A Study on the Target Search Logic in the ASW Decision Support System (대잠전 의사결정지원 시스템에서 표적 탐색 논리 연구)

  • Cho, Sung-Jin;Choi, Bong-Wan;Jeon, Jae-Hyo
    • Journal of the Korea Institute of Military Science and Technology
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    • v.13 no.5
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    • pp.824-830
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    • 2010
  • It is not easy job to find a underwater target using sonar system in the ASW operations. Many researchers have tried to solve anti-submarine search problem aiming to maximize the probability of detection under limited searching conditions. The classical 'Search Theory' deals with search allocation problem and search path problem. In both problems, the main issue is to prioritize the searching cells in a searching area. The number of possible searching path that is combination of the consecutive searching cells increases rapidly by exponential function in the case that the number of searching cells or searchers increases. The more searching path we consider, the longer time we calculate. In this study, an effective algorithm that can maximize the probability of detection in shorter computation time is presented. We show the presented algorithm is quicker method than previous algorithms to solve search problem through the comparison of the CPU computation time.

Detection of Xanthomonas axonopodis pv. aurantifolii and Xanthomonas axonopodis pv. citrumelo by Triplex PCR

  • Yu, Sang-Mi;Lee, Se-Won;Lee, Seung-Don;Park, Eun-Woo;Lee, Yong-Hoon
    • Research in Plant Disease
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    • v.18 no.2
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    • pp.129-132
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    • 2012
  • Citrus bacterial canker is an economically important disease affecting citrus production in many citrusgrowing areas and several pathotypes have been recognized within the Xanthomonas pathogens causing canker. In view of the containment of the disease, accurate identification of the causal bacterium is important. In this study, triplex PCR method was developed by using the previously reported primers. Two groups of primer combination, such as, one group including primers 2/3, J-pth1/J-pth2 and XACF/XACR, and another group 2/3, J-pth1/J-pth2 and Xac01/Xac02, were suitable for the detection and differentiation of X. a. pv. citri $A^w$, X. a. pv. aurantifolii B and C, and X. a. pv. citrumelo E strains. Moreover, the primer combination of Xac01 and J-pth2 promised us to use as a specific primer set to detect X. a. pv. citrumelo E strain. The PCR methods developed in this study could be used for the rapid differentiation of Xanthomonas pathotypes of citrus.

Quantitative Analysis of the Seventeen Marker Components in Dangguisu-san Using Ultra-performance Liquid Chromatography Coupled to Electrospray Ionization Tandem Mass Spectrometry (LC-MS/MS를 이용한 당귀수산 추출물 중 17종 성분의 함량분석)

  • Seo, Chang-Seob;Shin, Hyeun-Kyoo
    • YAKHAK HOEJI
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    • v.58 no.3
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    • pp.158-164
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    • 2014
  • Dangguisu-san is a well-known traditional Korean herbal medicine prescription and has been widely used to treat ecchymosis, blood stagnation, and pain resulting from physical shock in Korea. In this study, an ultra-performance liquid chromatography-electrospray ionization-mass spectrometer method was established for the simultaneous determination of the 17 biomarker components in Dangguisu-san. All analytes were separated on an UPLC BEH $C_{18}$ ($100{\times}2.1$ mm, $1.7{\mu}m$) column and maintained at $45^{\circ}C$. The mobile phase consisted of two solvent systems, 0.1% (v/v) formic acid in water (A) and acetonitrile (B) by gradient flow. The injection volume was $2.0{\mu}l$ and the flow rate was 0.3 ml/min with detection at mass spectrometer. Calibration curves of the 17 biomarker components were acquired with $r^2$ values ${\geq}0.9951$. The values of limit of detection and quantification of all analytes were 0.02~6.32 ng/ml and 0.05~18.95 ng/ml, respectively. The amounts of the 17 components in Dangguisu-san sample were $3.17{\sim}13,224.50{\mu}g/g$.

Olfactory Dysfunction in Chromium Exposed Workers (크롬 취급 근로자의 후각장애에 관한 조사연구)

  • Yu, Yeong-Jin;Ohm, Sang-Hwa;Lee, Jong-Tae;Yu, Byung-Chul;Jung, Kui-Oak;Cho, Kyu-Il;Pai, Ki-Tack
    • Journal of Preventive Medicine and Public Health
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    • v.28 no.3 s.51
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    • pp.678-689
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    • 1995
  • Chromium is one of the representative toxic substance by occupational exposure which damage the mucosa of respiratory tract including nasal septal perforation. The aim of this study is to evaluate the effect of chromium exposure on olfactory function and to obtain the fundamental information about chromium exposure. The authors performed olfactory function test, laboratory tests and questionnaire interview on the subject of three groups, that is, two exposed groups and one nonexposed group from May 1 to June 30, 1994. Exposed group 1 was 15 male workers without nasal septal perforation, exposed group 2 was 15 male workers with nasal septal perforation among 103 workers in 22 chromium plating factories, and nonexposed group was 15 male medical students. The gathered informations were histories of chromium exposure, habits of smoking and alcohol drinking, the concentrations of chromium in serum and urine, and asparate aminotransferase(AST), alanine aminotransferase(ALT), gamma-glutamyl transferase, etc. Olfactory function was checked by T and T olfectometer using phenyl ethyl alcohol(material A), methyl cyclopentenolone(material B), iso-valeric acid(material C), $\gamma$-undecalactone(material D), skatole(material E) and the results were expressed by detection threshold(DT) and recognition threshold(RT). There was a significant difference between exposed groups and nonexposed group in A, B, C, D, E substances by DT and in A, B, C, D substances by RT(P<0.01). The degree of olfactory dysfunction was highest in the exposed group 2 and lowest in the nonexposed group in all five substances by DT and it was same in A B, D substances RT and the difference of RT and DT. As summary, olfactory dysfunction by chromium exposure was recognized and the degree of olfactory dysfunction was higher in the exposed group with nasal septal perforation. Therefore, it would be helpful to apply olfactory function test for the early detection of olfactory dysfunction, and this test would be considered as the basic tool within workers' compensation system.

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Survival Rates of Breast Cancer Patients Aged 40 to 49 Years according to Detection Modality in Korea: Screening Ultrasound versus Mammography

  • So Yeon Won;Hyung Seok Park;Eun-Kyung Kim;Seung Il Kim;Hee Jung Moon;Jung Hyun Yoon;Vivian Youngjean Park;Seho Park;Min Jung Kim;Young Up Cho;Byeong-Woo Park
    • Korean Journal of Radiology
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    • v.22 no.2
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    • pp.159-167
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    • 2021
  • Objective: The aim of this study was to compare the survival rates of Korean females aged 40 to 49 years with breast cancer detected by supplemental screening ultrasound (US) or screening mammography alone. Materials and Methods: This single-institution retrospective study included 240 patients with breast cancer (mean age, 45.1 ± 2.8 years) detected by US or mammography who had undergone breast surgery between 2003 and 2008. Medical records were reviewed for clinicopathologic characteristics and detection methods. Disease-free survival (DFS) and overall survival (OS) were compared between patients with breast cancer in the US and mammography groups using the log-rank test. Multivariable cox regression analysis was used to identify independent variables associated with DFS and OS. Results: Among the 240 cases of breast cancer, 43 were detected by supplemental screening US and 197 by screening mammography (mean follow-up: 7.4 years, 93.3% with dense breasts). There were 19 recurrences and 16 deaths, all occurring in the mammography group. While the US group did not differ from the mammography group in tumor stage, the patients in this group were more likely to undergo breast-conserving surgery and radiation therapy than the mammography group. The US group also showed better DFS (p = 0.016); however, OS did not differ between the two groups (p = 0.058). In the multivariable analysis, the US group showed a lower risk of recurrence (hazard ratio, 0.097; 95% confidence interval, 0.001-0.705) compared to the mammography group. Conclusion: Our study found that Korean females aged 40-49 years with US-detected breast cancer showed better DFS than those with mammography-detected breast cancer. However, there were no statistically significant differences in OS.