• Journal of Nutrition and Health
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• v.22 no.6
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• pp.423-437
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• 1989

A 4-week energy balance study was conducted to estimate the energy expenditure (EE) of 16 college age men and women, 20 to 26 year of age, by measurement of energy intakes and changes in body energy(BE) content(intake/balance technique), keeping their normal living pattern and maintenance body weight. Energy intake was measured by bomb calorimetry and estimated by food table. Fecal energy loss was calculated from nitrogen excreted. Fat mass was determined from body density estimated from skinfold tickness. 1) Gross energy (GE) intakes calculated from food table was not only 13.4% lower than those of bomb calorimetry but also lower 4 and 5% than metabolizable energy(ME) intakes for the male and female subjects, respectively. 2) Fecal energy loss was 7.2% and 6.9% proportion of the gross energy intake for the male and female subjects, respectively. 3) Mean daily metabolizable energy intakes estimated by subtract fecal and urinary energy loss was 2467kcal for the male subjects and 1897kcal for the female subjects. 4) Total body energy change estimated from body composition change over 31 days was decreased 7672kcal for the male subjects and 2689kcal for the female subjects. 5) Mean daily energy expenditure was 2714kcal (45kcal/kg of body weight) for the male subjects and 1984kcal(40kcal/kg of body weight) for the female subjects. 6) The estimated energy expenditure of college-age subjects in this study provide evidence to support the Recommended Dietary Allowances for energy of Korean normal adult.

• Journal of Physiology & Pathology in Korean Medicine
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• v.28 no.1
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• pp.53-58
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• 2014

The object of this study was to evaluate the single dose toxicity of Choweseuncheng-tang (CWS), a polyherbal formula have been traditionally used as prevention or treatment agent for various diseases as Tae-eumin prescription on Korean medicinal theory, Sasang-euihak, in male and female mice. Aqueous extracts of CWS (yield = 11.00%) was administered to female and male mice as an oral dose of 2,000, 1,000 and 500 mg/kg (body weight) according to the recommendation of Korea Food and Drug Administration (KFDA) Guidelines. Animals were monitored for the mortality and changes in body weight, clinical signs and gross observation during 14 days after dosing, upon necropsy; organ weight and histopathology of 14 principle organs were also examined. As results, we could not find any CWS treatment related mortality and clinical signs, changes in the body and organ weights, gross findings and changes in histopathology of principle organs, except for some dose-independent accidental findings. The results obtained in this study suggest that the 50% lethal dose and approximate lethal dose of CWS aqueous extracts in both female and male mice were considered as over 2,000 mg/kg, the limited highest dosage recommended by KFDA Guidelines, and can be safety used in clinics.

• Biomolecules & Therapeutics
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• v.8 no.2
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• pp.167-170
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• 2000

The present study was conducted to investigate the acute toxicity of plant sterol ester by a single oral dose in Sprague-Dawley rats. Ten males and 10 females aged 5 weeks were randomly assigned to two groups of 5 rats each and were administered by gavage at dose level of 0 or 20 ml/kg body weight. Parameters measured during the 14-day observation period were mortality, clinical signs, body weight changes, and gross findings. No mortality was observed in the present study. Treatment-related clinical signs, such as pasty stool and diarrhea, were observed on the day of treatment and these signs resulted in soiled fur on day 1 after the treatment. However, no clinical signs were observed on days 2-14 after the treatment. There was no significant difference in body weight changes between the control and treatment groups. At necropsy on day 14 after the treatment, no treatment-related gross findings were observed in the treatment group. Based on these results, it was concluded that a single oral dose of plant sterol ester induced pasty stool and diarrhea in Sprague-Dawley rats at dose level of 20 ml/kg and that the lethal doses were considered to be over 20 ml/kg for both sexes.

• The Journal of Korean Obstetrics and Gynecology
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• v.27 no.1
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• pp.28-40
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• 2014

Objectives: This study was to evaluate the single dose toxicity of Sobokchuko-tang (SBC) in male and female mice. Methods: Aqueous extract of SBC (yield=6.60%) was administered to female and male mice as an oral dose of 2,000, 1,000 and 500 mg/kg (body weight) according to the recommendation of Korea Food and Drug Administration (KFDA) Guidelines. Animals were monitored for the mortality and changes in body weight, clinical signs and gross observation during 14 days after dosing, upon necropsy; organ weight and histopathology of 14 principle organs were also examined. Results: we could not find any SBC treatment related mortality and clinical signs, changes in the body and organ weights, gross findings and changes in histopathology of principle organs, except for pharmacological immunomodulatory effects related findings including significant increases of submandibular lymph node weights, hypertrophy and hyperplasia of lymphoid cells in the submandibular lymph nodes restrictly detected in 2,000 mg/kg treated female and male mice with some sporadic accidental findings. Conclusions: The results obtained in this study suggest that the 50% lethal dose and approximate lethal dose of SBC aqueous extracts in both female and male mice were considered as over 2,000 mg/kg, the limited highest dosage recommended by KFDA Guidelines, and can be safety used in clinics.

• The Journal of Korean Medicine
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• v.44 no.3
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• pp.29-38
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• 2023

Objectives: This study aimed to investigate the acute oral toxicity of 77 herbal formulas and performed in male and female Sprague-Dawley (SD) rats as per the guidelines mentioned in Ministry of Food and Drug Safety. Methods: Each sex of SD rat were administered a single dose (2000 or 5000 mg/kg) of 77 herbal formulas via oral gavage; the control group received vehicle only. After administration, the mortality, clinical signs, gross findings, and body weight were followed up for 15 days. Results: After administration of 77 herbal formulas, mortality, clinical signs, body weight changes, and gross findings related to the test substances were not observed in both male and female groups. Conclusion: Our results demonstrate the single-dose oral administration of 77 herbal formulas produced no mortality indicating the approximate lethal dose is greater than 2000 or 5000 mg/kg body weight.

• Transactions of the Korean Society of Automotive Engineers
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• v.15 no.2
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• pp.58-64
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• 2007

As the concerns regarding global worming were increased, the pressure of greenhouse gas(GHG) emission reduction on mobile source was also increased. Carbon dioxides contribute over 90% of total GHG emission and the mobile source occupies about 20% of this $CO_2$ emission. Therefore automotive exhaust is suspected to be one of the major reasons of the rapid increase in greenhouse effect gases in ambient air. In this study, in order to investigate $CO_2$ emission characteristics from gasoline passenger cars(PC), which is the most dominant vehicle type in Korea, 106 vehicles were tested on the chassis dynamometer. $CO_2$ emissions and fuel efficiency were measured. The emission characteristics by displacement, gross vehicle weight, vehicle speed and CVS-75/vehicle speed mode were discussed. Test modes were vehicle speed modes and CVS-75 mode that have been used to develop emission factors and to regulate for light-duty vehicle in Korea. It was found that $CO_2$ emissions showed higher large displacement, heavy gross vehicle weight, low vehicle speed and CVS-75 mode than small displacement, light gross vehicle weight, high vehicle speed and vehicle speed mode, respectively. From these results, correlation between $CO_2$ emission and fuel efficiency was also determined. The results of this study will contribute to domestic greenhouse gas emissions calculation and making the national policy for climate change.

• Korean Journal of Environmental Agriculture
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• v.38 no.3
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• pp.145-153
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• 2019

BACKGROUND: The 'Gamhong' cultivar, middle season apple with big fruit size and high soluble solid content, has been bred in Korea. However, it was hard to cultivate the cultivar in Korea by serious bitter pit. The relationships between shoot growth, fruit size, and bitter pit may be affected by crop load. This study was conducted for 2 years (7~8 years after planting) to investigate vegetative growth, fruit quality, bitter pit incidence, return bloom, and gross income for optimum crop load of 'Gamhong'/M.9 adult apple tree. METHODS AND RESULTS: The crop load was assigned to 4 different object ranges as follow: 45~64, 65~84, 85~104, and 105~124 fruits per tree. The vegetative growth, average fruit weight, percentage of fruits heavier than 375 g, soluble solid content, and return bloom increased significantly at the crop load range of 45~64 fruits. However, the lowest total gross income per tree may have been caused by the highest bitter pit incidence and the lowest yield per tree in any other crop load range. The total gross income and yield per tree increased significantly at the crop load range of 105~124 fruits and return bloom dropped to 40%, and hence it was possible to occur biennial bearing. It was 85~104 fruits that biennial bearing did not occur and total gross income was as high as the crop load range of 105~124 fruits. Also, the yield of high grade fruits per tree, with fruit weight of 400~499 g and none bitter pit on fruit surface, was highest at the crop load range of 85~104 fruits, compared to other crop load range. CONCLUSION: In considering fruit size, bitter pit incidence, return bloom, and gross income, the optimum crop load range of 'Gamhong'/M.9 adult apple tree in high density orchard was 85~104 fruits per tree.

• The Journal of Korean Medicine
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• v.32 no.3
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• pp.18-24
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• 2011

Objective: Leejung-tang (Rechu-to in Japanese) is a traditional Korean herbal formula used for treatment of gastrointestinal disorders such as vomiting, stomach pain, chronic gastritis and gastrointestinal ulceration. The present study was carried out to investigate the potential acute toxicity of Leejung-tang water extract (LJT) by a single oral dose in Crl:CD (SD) rats in compliance with current guidelines. Methods: In the preliminary study, there were no adverse effects such as death, clinical signs, and body weight changes at dose levels of 500, 1000, and 2000 mg/kg/day body weight. Based on the results, a dose of 2000 mg/kg was selected as the toxicological limited dose. LJT was administered once by gavage to male and female rats at dose levels of 0 and 2000 mg/kg bodyweight. During the study period, mortalities, clinical findings, and body weight changes were observed for 14 days following the administration. On day 14 after the treatment, the animals were sacrificed by carbon dioxide overdose and complete gross postmortem examinations were performed. Results: In present study, no treatment-related deaths were observed. There were no adverse effects on clinical signs and body weight changes. In addition, there were no observed gross findings in all groups except for a kidney cyst in the 2000 mg/kg/day female group. Conclusion: The results indicated that LJT did not induce toxic effects at a dose level up to 2000 mg/kg in rats and its median lethal dose ($LD_{50}$) was considered to be over 2000 mg/kg/day body weight for both genders.

• Biomolecules & Therapeutics
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• v.18 no.1
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• pp.111-121
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• 2010

In order to investigate the preliminary repeat oral dose toxicity and to determine the highest dosage for further 4-week repeated dose toxicity test, Low Molecular Weight Fucoidan (LMF) has been showed various pharmacological effects, was orally administered to female and male rats, once a day for 14 days at dose levels of 2,000, 1,000, 500 and 0 (vehicle control) mg/kg (body weights) in a volume of 10 ml/kg. The mortality and changes on the body weights, clinical signs, hematology, serum biochemistry and gross observations were monitored with organ weight and histopathology of principle organs. As the results of 14-day repeated oral treatment of LMF, no LMF treatment related mortalities were detected up to 2,000 mg/kg in both male and female rats, respectively. In addition, no noticeable changes on the body weight and clinical signs were detected except for significant decreases on the body weights and gains restricted to male 2,000 mg/kg treated groups as compared with male vehicle control. No meaningful changes on the organ weights, hematological, serum biochemistrical, gross and histopathological findings were observed. Therefore the highest dosage in the 4-week repeated dose toxicity test is suggested as 2,000 mg/kg in both female and male rats, respectively.

• Journal of Navigation and Port Research
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• v.43 no.1
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• pp.16-22
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• 2019

This study proposed estimation methods for weight and center of gravity of small fishing vessels. Weights loaded on small fishing vessels were divided into fixed weights such as crew, fishing gear, and variable weights such as fuel, fresh water, provision, bait, and fish. Based on statistical analyses with weight data of several small fishing vessels, weight, longitudinal center of gravity (LCG), vertical center of gravity (KG) of each item were represented as linear functions of vessel gross tonnage. In addition, weighting factors of variable weights were added on estimation formulas in accordance with vessel loading conditions, e.g. full load departure condition. Estimation methods were verified using actual small fishing vessel data.