• 전자공학회논문지A
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• 제29A권7호
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• pp.64-72
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• 1992

VLSI 전송선로의 커패시턴스를 3차원으로 계산하였다. Green's function과 표면전하밀도의 곱의 형태로 주어지는 적분식을 풀어서 커패시턴스를 구하였다. 이때, 표면전하밀도는 도체의 표면을 균일한 면적을 갖는 미소 면적소로 나누어 주었을 때 각각의 면적소 내에서는 일정한 상수값을 갖는다고 가정하였다. 지금까지의 Green's function을 이용한 적분방법에서는 적분식의 계산을 Fourier 적분의 형태로 변환하여 계산하였기 때문에 계산과정에서 어느정도의 오차가 있을 수 밖에 없었지만, 본 논문에서는 Fourier 적분을 사용하는 대신에 이중 적분을 직접적으로 적분할 수 있는 방법을 제시하였다. 이 방법을 사용하여 적용한 결과를 기존의 결과들과 비교를 함으로써, 이의 정확성을 입증하였다.

• Journal of Plant Biotechnology
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• 제2권3호
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• pp.135-142
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• 2000

The activity of promoter sequences was evaluated in garlic cells using the $\beta$-glucuronidase (GUS) gene as a reporter. Histochemical GUS assay indicated transient GUS activity in leaf, callus and root cells 48 hours after particle bombardment transformation. Quantitative fluorometric assays in extracts of transformed leaves demonstrated that the CsVMV promoter induced the highest level of gene expression, which was, on average, ten fold the level induced by CaMV35S and by the Arabidopsis Act2 promoters and two fold the level expression observed with a construct containing a double CaMV35S plus the untranslated leader sequence from AMV. No activity or very low levels were observed when cells were transformed with plasmids rontaining the typical monocot promoters, Actl, from rice or the Ubi-1, from maize. The green fluorescent protein (GFP) was also tested as a marker gene for garlic transformation. Intense fluorescence was observed in leaf, callus and root cells transformed with a construct containing the gfp gene under control of the CaMV35 Promoter. No fluorescence was detected when the gfp was under control of the Ubi-1 promoter.

• 한국작물학회지
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• 제58권2호
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• pp.119-127
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• 2013

The interaction between Agrobacterium and soybean has been studied at the transcriptome level but not at the metabolic level. However, it is necessary to investigate the difference in metabolites between susceptible and non-susceptible cultivars for high efficiency transformation. We investigated the difference in metabolites from sonicated soybean cotyledons of Korean cultivars and Bert cultivar. To identify difference in metabolites, sonicated extracts were analysed by Fourier transform ion cyclotron resonance mass spectrometry (FT-ICR/MS). The soybean cultivars were classified by susceptibility using green fluorescent protein expression. We found a difference in metabolites between the high susceptible and low susceptible cultivars. The FT-ICR/MS experimental m/z data of different metabolites were compared with theoretical m/z in KNApSAcK database. The candidate list was made using KNApSAcK and focused on phenolic compounds. These candidate metabolites are speculated to influence factors in the interaction. This list of candidates may be useful to investigate the interaction between Agrobacterium and plants to increase transformation efficiency.

• The Plant Pathology Journal
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• 제21권1호
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• pp.13-20
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• 2005

Chlorella viruses are large icosahedral, plaque-forming, dsDNA viruses that infect certain unicellular, chlorellalike green algae. The genomic DNA of over 300 kb contains many useful genes and promoters. Over 40 chlorella viruses have been isolated from fresh water in Korea since 1998. The viruses were amplified initially in chlorella strain NC64A, and pure isolates were obtained by repeated plaque isolation. SDS-PAGE analysis revealed similar but distinct protein patterns, both among the group of purified viruses and in comparison with the prototype chlorella virus PBCV-1. Digestions of the 330- to 350-kb genomic DNAs with 10 restriction enzymes revealed different restriction fragment patterns among the isolates. The tRNA-coding regions of 8 chlorella viruses were cloned and sequenced. These viruses contain 14-16 tRNA genes within a 1.2- to 2-kb region, except for the SS-1 isolate, which has a 1039-bp spacer in a cluster of 11 tRNA genes. Promoter regions of several early genes were isolated and their activities were analyzed in transformed chlorella. Some promoters showed stronger activity than commonly used CaMV 35S promoter and chlorella transformation vectors for heterologous protein are beings constructed using these promoters.

• 센서학회지
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• 제21권1호
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• pp.28-33
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• 2012

The visual sensing of human hands plays an important part in many man-machine interaction/interface systems. Most existing visionbased hand detection techniques depend on the color cues of human skin. The RGB color image from a vision sensor is often transformed to another color space as a preprocessing of hand detection because the color space transformation is assumed to increase the detection accuracy. However, the actual effect of color space transformation has not been well investigated in literature. This paper discusses a comparative evaluation of the pixel classification performance of hand skin detection in four widely used color spaces; RGB, YIQ, HSV, and normalized rgb. The experimental results indicate that using the normalized red-green color values is the most reliable under different backgrounds, lighting conditions, individuals, and hand postures. The nonlinear classification of pixel colors by the use of a multilayer neural network is also proposed to improve the detection accuracy.

• 한국가시화정보학회지
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• 제8권4호
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• pp.26-30
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• 2010

A PTV algorithm was constructed using a linear transformation, in which the merits of the conventional PIV and PTV were adopted. In PIV calculations, the obtained velocity vectors are affected by the filtering effects by its calculation principle. PTV techniques are widely used for their excellences of measuring small scaled flows, such as nano and bio flows. However, PTVs produce vector errors due to interpolation process. To overcome these problems, a hybrid PTV algorithm was constructed by combining PTVs' and PIVs' benefits using a linear transformation. The Taylor-Green vortex flows were generated for the tests of vorticity calculations. The conventional gray-level cross-correlation PIV technique and 2-Frame PTV technique were tested for the same flows for comparisons with those obtained by the constructed hybrid algorithm. The excellence of the constructed hybrid algorithm was validated through an actual experiment on the cylinder wake.

• Journal of Electrical Engineering and information Science
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• 제3권3호
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• pp.330-335
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• 1998

In this paper, a new light-value approaching method to enhance a color image by excluding the effect of incident illumination is proposed. The method uses the fundamental vector transformation in which an estimated color of illumination is rotated to the white color of natural daylight. Then the transformed red, green, and blue values of each pixel are nonlinearly mapped into the 8-bit values to enhance intensity and saturation in the dark portion of the image. The proposed algorithm can produce the enhanced color image fast and efficiently without any space conversion or noticeable distortion.

• Journal of Plant Biotechnology
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• 제33권2호
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• pp.75-84
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• 2006

Heteroplasmic rice plastid transformant was generated using suspension cells as bombardment materials. PCR analyses confirmed incorporation of aadA and cp4-epsps genes into the rice plastid genome by homologous recombination events via the flanking sequences of the trnI and trnA. Transplastomic calli were actively proliferated when cultured on AAM2 medium supplemented with various concentrations (500-3000 mg/L) of streptomycin in dark condition, and transplastomic suspension cells showed resistance to nonselective herbicide, glyphosate. Through 'agarose pie selection' method, heteroplastomic calli, containing considerably high level of transplastome and expressing the CP4 EPSPS protein, were obtained. They were further regenerated to green shoots with healthy roots.

• 대한약학회:학술대회논문집
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• 대한약학회 2003년도 Proceedings of the Convention of the Pharmaceutical Society of Korea Vol.2-2
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• pp.145.3-146
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• 2003

Transgenic Eleutherococcus senticosus plants were prepared by introducing the genes for squalene synthase (SQS), hygromycin phosphotransferase (HPT) and green fluorescent Protein (GFP) through Agrobacterium-mediated transformation. The enzyme, SQS, represents a putative branch point in the isoprenoid pathway capable of diverting carbon flow specifically to the biosynthesis of phytosterol and oleanolic acid. The full SQS gene was isolated from P. ginseng roots. Early globular embryo clusters developed from embryogenic callus were used as the explant source. (omitted)

• Advances in nano research
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• 제1권4호
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• pp.211-218
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• 2013

A low-cost, green and reproducible citric acid assisted synthesis of nanocrystalline $Al_{0.5}Ag_{0.5}TiO_3$ (n-AAT) powder is reported. X-ray, FTIR, energy dispersive X-ray, transmission electron microscopy and scanning electron microscopy analyses are performed to ascertain the formation of n-AAT. X-ray diffraction data analysis indicated the formation of monoclinic structure. Spherical shaped particles having the sizes of 3-15 nm are found. The mechanism of nano-transformation for the soft-chemical synthesis of n-AAT has been explained using simple organic chemistry rules and nucleation and growth theory. Dielectric study revealed that AAT ceramic might be a suitable candidate for capacitor applications.