• 한국식품영양학회지
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• 제22권2호
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• pp.279-285
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• 2009

맥문동을 열수 추출하여 전자공여능, 항산화력 및 항균력을 시험한 결과는 다음과 같다. 1. 전자공여능은 대조구인 BHA가 85 Vit C가 92, 녹차추출물(GT) 1 Brix%는 88, 반건조 맥문동 추출물(MDL) 10 Brix%는 65, 건조 맥문동 추출물(DL) 3 Brix%는 79를 나타내었다. 2. 지질과산화물 생성 억제 효과는 녹차추출물(GT) > 건조맥문동 추출물(DL) > 반건조 맥문동 추출물(MDL) 순으로 녹차추출물(GT)이 가장 높은 것으로 나타났다. 3. S. aureus에 대한 각 시료의 항균력은 BHA > 녹차추출물(GT) 순이며, 반건조 맥문동 추출물(MDL)과 건조 맥문동 추출물(DL)은 나타나지 않았다. E. coli에 대한 각 시료의 항균력 또한 BHA > 녹차추출물(GT) 순이며, 반건조 맥문동 추출물(MDL)과 건조 맥문동 추출물(DL)은 나타나지 않았다. 4. 맥문동 열수추출물의 전자공여능과 지질과산화물 생성 억제 효과는 반건조추출물(MDL)보다 건조추출물(DL)이 우수한 것으로 나타났으며, 항균력에서는 S. aureus와 E. coli에 대하여 항균력이 나타나지 않은 것으로 나타났다.

• 한국식품저장유통학회지
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• 제16권6호
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• pp.924-928
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• 2009

녹차에 관한 최근까지의 연구결과에서, 녹차는 생리활성의 촉진작용뿐아니라 항암, 항산화, 고혈압 억제효과, 체중 조절 등 다방면의 약리효과를 보이는 것으로 보고되고 있다. 아울러, 녹차의 항균 및 항진균 작용에 관한 연구결과도 발표되고 있으며, 본 연구팀에 의해서도 녹차추출물의 항균작용을 확인한 바 있어, 본 연구에서는 탁월한 항균작용을 소유하고 있는 녹차분획물질을 분리하여 그 분자구조를 동정하는데 목적이 있다. 따라서, 녹차추출물에서 탁월한 항균력을 가지는 항균활성물질을 HPLC 분리방법 및 NMR 분석법으로 분리 동정한 결과, 각각 (-)-epicatechin gallate 및 (-)-epigallocatechin gallate로 확인되었다.

• BMB Reports
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• 제38권5호
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• pp.563-570
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• 2005

Tamoxifen citrate (TAM), is widely used for treatment of breast cancer. It showed a degree of hepatic carcinogenesis. The purpose of this study was to elucidate the antioxidant capacity of green tea (Camellia sinensis) extract (GTE) against TAM-induced liver injury. A model of liver injury in female rats was done by intraperitoneal injection of TAM in a dose of $45\;mg\;Kg^{-1}\;day^{-1}$, i.p. for 7 successive days. GTE in the concentration of 1.5%, was orally administered 4 days prior and 14 days after TAM-intoxication as a sole source of drinking water. The antioxidant flavonoid; epicatechin (a component of green tea) was not detectable in liver and blood of rats in either normal control or TAM-intoxicated group, however, TAM intoxication resulted in a significant decrease of its level in liver homogenate of tamoxifen-intoxicated rats. The model of TAM-intoxication elicited significant declines in the antioxidant enzymes (glutathione-S-transferase,glutathione peroxidase, superoxide dismutase and catalase) and reduced glutathione concomitant with significant elevations transaminase) levels. The oral administration of 1.5% GTE to TAM-intoxicated rats, produced significant increments in the antioxidant enzymes and reduced glutathione concomitant with significant decrements in TBARS and liver transaminases levels. The data obtained from this study speculated that 1.5% GTE has the capacity to scavenge free radical and can protect against oxidative stress induced by TAM intoxication. Supplementation of GTE could be useful in alleviating tamoxifen-induced liver injury in rats.

• Natural Product Sciences
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• 제19권2호
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• pp.166-172
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• 2013

Caffeine and epigallocatechin gallate (EGCG) are major constituents of green tea, the leaves of Camellia sinensis (Theaceae). Although EGCG is well known for diverse beneficial effect, caffeine is sometimes harmful with adverse effects. Therefore, the extraction efficiency was investigated using different extraction method such as extraction solvent, extraction time, extraction method, and repeated extraction. The content of EGCG and caffeine in green tea extract was quantitated by HPLC analysis. The extraction condition exerted difference on the extraction yield. The content of EGCG was also affected by different extraction condition. Especially, the extraction solvent greatly affected the content of EGCG in the extract. However, the content of caffeine was less affected compared to that of EGCG. The inhibitory effect of green tea extract on pancreatic lipase was almost similar regardless of extraction condition. Taken together, optimization of extraction condition will provide best efficacy for further development of green tea as anti-obesity therapeutics.

• 대한화장품학회지
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• 제44권1호
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• pp.67-72
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• 2018

녹차(Camellia sinensis L.)는 약용으로도 널리 사용되어 왔다. 하지만, 대부분의 연구는 녹차 잎을 연구의 대상으로 이루어졌으며, 지금까지도 잎 외의 다른 부분은 연구가 많이 이루어지지 않았다. 본 연구에서는 외부환경의 미세먼지(particulate matter 2.5; PM2.5)에 의한 normal human epidermal keratinocytes (NHEKs)의 손상을 뿌리 추출물이 효과적으로 보호할 수 있는 효과를 확인하였다. 30년 된 녹차뿌리 표본은 아모레퍼시픽의 Dolsongi tea field에서 채취되었으며 70%의 에탄올로 추출한 녹차 뿌리 추출물은 총 순수 사포닌 함량이 54%로, 인삼 추출물보다 더 많은 사포닌을 함유한 것을 확인하였다. 그리고 PM2.5에 의한 손상으로부터의 보호 효과를 평가하기 위해 건선환자의 특이적 바이오 마커인 IL-36G를 관찰한 결과 IL-36G mRNA 발현량은 PM2.5 처리시 녹차 뿌리 추출물 처리를 통해 IL-36G 식의 유의적인 감소를 확인할 수 있었다. 결론적으로, 30년 된 녹차뿌리 추출물은 사포닌 함량이 높은 자연 계면 활성제로 사용될 수 있으며, 또한 피부질환마커인 IL-36G 발현의 억제를 통해 PM2.5에 의해 유발되는 손상에 대한 보호 효과가 있음을 알 수 있었다.

• Food Science and Biotechnology
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• 제17권3호
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• pp.464-469
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• 2008

In human beings, it is known that there is a correlation between the occurrence of acne and the ability to suppress sebum. Sebosuppression may be related to the inhibition of sebocyte proliferation, differentiation, and lipogenesis in sebaceous glands. To investigate the skin sebosuppressive activity of green tea extract, the in vivo effects of its flavonoid compounds on the androgen-dependent stimulation of pigmented macules in hamsters and performed in vitro experiments with human primary sebocytes were examined. Our results imply a dual activity of skin sebosuppression by green tea flavonoids; some catechins including epigallocatechin-3-gallate (EGCG) and gallocatechin-3-gallate (GCG) may reduce the differentiation of sebocytes by inhibiting PPAR-${\gamma}1$ mRNA expression, whereas some flavonol glycosides including kaempferol may inhibit lipogenesis in sebaceous glands by decreasing levels of the mature form of sterol-sensitive response elements binding protein-1c (SREBP-1c). Therefore, green tea is a potentially effective material for use in the development of health foods or cosmetics for skin sebosuppression.

• 한국식품영양학회지
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• 제31권1호
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• pp.89-93
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• 2018

In this study, we investigated the nutritional composition including proximate, amino acid, vitamin, minerals, and the antioxidant activity of green tea (Camellia sinensis) pollen grains collected by Apis mellifera bees, for use as a health food. The crude protein and fat content was estimated at 26.14% and 3.49%, respectively. Eighteen amino acids were identified in green tea pollen, including 8 essential amino acids and 10 non-essential amino acids. The predominant amino acids were glutamic acid, proline and aspartic acid accounting for about 33.3% of total free amino acids. The concentration of vitamin C was the highest value of 35.7%, followed by $B_3$ and $B_2$ among the detected vitamins. The predominant minerals were potassium (790.32 mg/100g), followed by phosphorus (707.52 mg/100g) and sulfur (302.67 mg/100g), whereas copper, zinc and sodium were detected as minor elements. The antioxidant activity and phenolic content accounted for 33.8% at $500{\mu}g/mL$ extract and $2.55 {\mu}g/mg$, respectively.

• 생약학회지
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• 제50권3호
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• pp.198-204
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• 2019

A facile and convenient method was developed for the mass production of epigallocatechin gallate (EGCG) rich green tea extract (Er-GTE). The Er-GTE was successfully obtained from the crude water extract of green tea by the combination of two step purification, i.e., a simple adsorption process on the cation exchange resins (Trilite SCR-B) followed by the chromatography with Diaion HP-20 resins. The green tea extract produced by water extraction under $45^{\circ}C$ was subjected to adsorb on the strongly acidic cation exchange resin, Trilite SCR-B. The eluate passed through the resin was reabsorbed on Diaion HP-20 resin, which was subjected to elute with a mixture of water and alcohol by conventional chromatographical manner. The EGCG content in Er-GTE was estimated above 97% by HP-LC analysis and the newly developed method was regarded as the most suitable and appropriate process for the mass production of epigallocatechin gallate rich green tea extract (Er-GTE).

• 한국환경농학회지
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• 제42권4호
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• pp.396-407
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• 2023

The golden apple snail (Pomacea canaliculata) has been utilized as a natural and eco-friendly control of weeds in rice paddy fields. However, P. canaliculata can damage other crops. In this study, the effectiveness of plant extracts from various natural sources that are reportedly effective against pests in the control of P. canaliculata was investigated. The four plant extracts were effective against P. canaliculata and ranked in descending order as green tea seed (Camellia sinensis) > root of red spider lily (Lycoris radiata) > leaves of tobacco (Nicotiana tabacum) > root of sophora (Sophora flavescens). The mortality rate of P. canaliculata was increased using 200 to 2000 mg/kg of green tea seed powder. However, shrubby sophora root extract did not significantly increase the mortality rate. The LC₅₀ and LC₉₀ of green tea seed, tobacco leaves, shrubby sophora root, and red spider lily root were 900 and 2800 mg/L, 956 and 2320 mg/L, 2162 and 5325 mg/L, and 512 and 1054 mg/kg, respectively. The LC₅₀ and LC₉₀ of ground powder of C. sinensis, N. tabacum, S. flavescens and L. radiata were 248 and 646 mg/L, 403 and 733 mg/L, 409 and 905 mg/L, and 493 and 1141 mg/L, respectively. The findings indicate the remarkable control potency of green tea seeds against the golden apple snail. An organic material incorporating the four plant powders may help control green apple snail in an ecosystem-friendly manner.

• 생명과학회지
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• 제19권7호
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• pp.928-933
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• 2009

노로바이러스는 인간에게 급성위장염을 일으키는 식중독 바이러스로, 최근 전세계적으로 노로바이러스 식중독 사고가 증가하고 있다. 따라서 본 연구에서는 다양한 생리활성 작용이 있다고 알려진 향신료, 차(茶), 식물성 한약재등 11종의 천연물질을 대상으로 노로바이러스의 대체모델인 feline calicivirus (FCV 에 대한 항바이러스 활성을 조사하였다. 각 추출물에 대한 항바이러스 활성은 end point dilution assay 방법인 50% tissue culture infectious dose ($TCID_{50}$)으로 바이러스 감염가를 측정하였다. 녹차(Camellia sinensis L.) 추출물의 경우 3.13 mg/ml의 농도에서 1 시간만에 FCV가 완전히 불활성화 되었으며, 홍화씨 (Carthamus tinctorius L.) 추출물은 6.25 mg/ml의 농도에서 5시간 만에 바이러스가 불활성화 되어 녹차추출물이 FCV에 대한 항바이러스 활성이 가장 뛰어난 것으로 조사되었으며, 농도 및 반응시간에 유의적으로 항바이러스 활성을 나타내는 것으로 관찰되었다. 녹차 및 홍화씨 추출물 등과 같은 천연식물 자원을 이용하여 FCV에 대한 항바이러스 활성을 조사한 결과는 궁극적으로는 노로바이러스에 의한 식중독 저감을 위한 기초자료에 이용될 수 있을 것으로 기대된다.