• Journal of Veterinary Clinics
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• v.27 no.5
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• pp.508-513
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• 2010

Cytokines are important mediators of the immune response, and quantitating cytokine mRNA is a highly sensitive and attractive method for measuring cytokine production. The objective of the current study was to develop and validate a SYBR green quantitative real-time reverse transcriptase PCR (qRT-PCR) assay for measuring canine cytokine mRNA. The optimal annealing temperatures ($T_a$) of the designed primers were $62^{\circ}C$ for interleukin (IL)-$1{\beta}$, IL-6 and IL-10; $60^{\circ}C$ for glyceraldehyde 3-phosphate dehydrogenase (GAPDH) and tumor necrosis factor (TNF)-${\alpha}$; and $58^{\circ}C$ for high mobility group box 1 (HMGB1). Primer efficiencies of all primers calculated for standard curve samples were between 97.1% and 102.6%. No evidence of secondary structure or primer-dimer formation was seen via melt-curve analysis or gel electrophoresis. The developed qRT-PCR assays are highly specific and sensitive and can be used to quantify gene expression levels of canine cytokines.

• Journal of Microbiology and Biotechnology
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• v.18 no.7
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• pp.1317-1325
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• 2008

Viral safety is an important prerequisite for clinical preparations of plasma-derived pharmaceuticals. One potential way to increase the safety of therapeutic biological products is the use of a virus-retentive filter. In order to increase the viral safety of human antihemophilic factor IX, particularly in regard to non-enveloped viruses, a virus removal process using a polyvinylidene fluoride membrane filter (Viresolve NFP) has been optimized. The most critical factor affecting the filtration efficiency was operating pH and the optimum pH was 6 or 7. Flow rate increased with increasing operating pressure and temperature. Recovery yield in the optimized production-scale process was 96%. No substantial changes were observed in the physical and biochemical characteristics of the filtered factor IX in comparison with those before filtration. A 47-mm disk membrane filter was used to simulate the process performance of the production-scale cartridges and to test if it could remove several experimental model viruses for human pathogenic viruses, including human hepatitis A virus (HAV), porcine parvovirus (PPV), murine encephalomyocarditis virus (EMCV), human immunodeficiency virus type 1 (HIV), bovine viral diarrhea virus (BVDV), and bovine herpes virus (BHV). Non-enveloped viruses (HAV, PPV, and EMCV) as well as enveloped viruses (HIV, BVDV, and BHV) were completely removed during filtration. The log reduction factors achieved were $\geq$6.12 for HAV, $\geq$4.28 for PPV, $\geq$5.33 for EMCV, $\geq$5.51 for HIV, $\geq$5.17 for BVDV, and $\geq$5.75 for BHV. These results indicate that the virus filtration process successfully improved the viral safety of factor IX.

• Proceedings of the Korean Institute of Building Construction Conference
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• 2012.05a
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• pp.337-338
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• 2012

As a rahmen structure, the connection among columns of Green frame is divided into three types such as sleeve, coupler (column pre and post installation), and bolt. The bolt type consists of six types according to steel frame shape and each type has different constructability, safety, structural performance, cost, and quality. Therefore, the analysis of comparison among the types is necessary. The objective of this study is to analyze the characteristics according to the shape of six bolt types to select the appropriate connection of Green frame. The results of this study can be used as a basic study for indentifying the characteristics of steel frame on site applying bolt type connection of Green frame. In addition, this study can be applicable to compare and analyze the performance and constructability of six bolt types in detail.

• Journal of the Architectural Institute of Korea Planning & Design
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• v.34 no.1
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• pp.71-81
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• 2018

The purpose of this study is to develop neighborhood assessment tools from the perspective of women in order to evaluate neighborhood environments and suggest better policies and planning practices for women-friendly neighborhood environments. To do so, we analyze correlations between women's quality of life and the neighborhood environment. Specifically, we gave female participants a mapping survey, interview, and questionnaire to collect data targeting women living in Onyang 2-dong and 5-dong in Asan city. First, we developed a neighborhood assessment tool derived from an extensive literature review and particular case studies. The mapping and interview surveys indicated that specific aspects neighborhood life needed to be improved in a similar area. In particular, women complained about Onyangoncheon station, a commercial area, and detached house area. Second, analysis of the neighborhood environmental factors affecting women's neighborhood satisfaction, crime safety, traffic and walking environment, accessibility of living facilities, green and public spaces and childcare environment were statistically significant. Of these, the inclusion of green and public spaces was the most influential factor. Third, the neighborhood environmental factors affecting women's neighborhood attachment, crime safety, traffic and walking environment, accessibility of living facilities, green and public spaces and childcare environment were statistically significant. Of these, accessibility of living facilities appeared to be the most influential factor. Fourth, the neighborhood environmental factors affecting women's housing mobility decisions, crime safety, traffic and walking environment, accessibility of living facilities, green and public spaces and childcare environment were statistically significant. Of these, childcare environment was the most influential factor.

• Korean Journal of Environmental Agriculture
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• v.32 no.4
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• pp.273-278
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• 2013

BACKGROUND: Total petroleum hydrocarbons (TPH), which are main materials of soil contamination by oil, are a term used for any mixture of hydrocarbons. Korea Ministry of Environment established the maximum permissible level of TPH in farmland by 500 mg/kg, and reported that the TPH level of soil in 266 installation such as gas station, transport company, and military unit ranged from 1,356 to 55,117 mg/kg and were much higher than the maximum permissible level in 2011. METHODS AND RESULTS: To determine the effect of TPH on crops, we investigated the effect of gasoline, kerosene, and diesel on the germination and radicle growth of mainly consumed crops. The germination rates of control in investigated all crops ranged from 80.0-100%. The germination and radicle growth in majority of investigated crops were not inhibited even at 2,500 mg/L. However, germination in onion, leek, and green perilla and radicle growth in leek, rape, tomato, and green perilla were significantly inhibited by increasing concentrations of gasoline, kerosene and diesel treatment. Germination and radicle growth inhibition of green perilla by kerosene and diesel were the highest, the percent inhibition at the 500 mg/L were 100 and 98.6%, 100 and 88.2%, respectively. 50% inhibition of germination in green perilla by kerosene and diesel were 39.96 and 29.87 mg/L, and 50% inhibition of radicle growth were 52.76 and 177.96 mg/L, respectively. Conclusion(s): These results suggest the possibility that the maximum permissible level of TPH might to be established general level with exception by crops.

• The Korean Journal of Pesticide Science
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• v.17 no.4
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• pp.419-427
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• 2013

The insecticidal activity of 124 plant essential oils and control efficacy of six experimental spray formulations (SF) containing 0.25, 0.5, 1, 2.5, 5, and 10% of the selected oils was examined against both nymph and adult of the citrus flatid planthopper, Metcalfa pruinosa using direct contact applications (leaf dipping and spray). Reponses varied according to dose (1,000 and 500 mg/L). When exposed at 1,000 mg/L for 24 h using leaf dipping assay, 19 essential oils showed strong mortality (100%) among 124 essential oils screened. At 500 mg/L, 100% mortality was observed in cinnamon technical, cinnamon green leaf, cinnamon #500, cassia tree, citronella java and pennyroyal followed by origanum, thyme white, grapefruit, savory, fennel sweet, aniseed and cinnamon bark showed considerable mortality (93.3-80%) against nymphs of M. pruinosa. The moderate mortality (73.3-60%) was found in thyme red, tagetes, calamus, lemoneucalptus and geranium. Oils applied as SF-10% sprays provided 100 % mortality against adult M. pruinosa. One hundred mortalities were achieved in cinnamon technical at >SF-0.5 formulation, in cinnamon #500, cinnamon green leaf and penny royal at >SF-2.5. To reduce the level of highly toxic synthetic insecticides in the agricultural environment, the active essential oils as potential larvicides could be provided as an alternative to control M. pruinosa populations.

• Korean Journal of Organic Agriculture
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• v.19 no.spc
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• pp.21-24
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• 2011

• Proceedings of the Korean Nuclear Society Conference
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• 2005.10a
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• pp.653-654
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• 2005

• Journal of Korean Society of Transportation
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• v.12 no.1
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• pp.55-73
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• 1994

The objective of this research is to evaluate the pedestrian signal time involving green and flashing green times. The minimum pedestrian green indication should give time for pedestrian to start crossing safely, and the flashing green indication should give time to complete the crossing. An average pedestrian crossing speed of 1.1(m/s) was estimated by analyzing the field data which was slower than the 1.2(m/s) currently used. Furthermore, the study proposed that design speed for the flashing green time should be slow speed for considerations pedestrian safety, not the average speed. The 0.78-1.01(m/s) of pedestrian speed was estimated at the elementary school areas that indicated 0.2(m/s) slower than the other areas. The pedestrian starting time (perception/reaction time) and time headway from front to back of herd was estimated to determine minimum pedestrian green time. the pedestrian starting time was estimated to determine minimum pedestrian green time. The pedestrian starting time was ranged 2.52-4.29 seconds. The time interval between the pedestrian rows was found to be 1.25-1.86 seconds, which declines as the pedestrian rows increases, The equation to calculate the pedestrian signal, which declines as the pedestrian rows increases. The equation to calculate the pedestrian signal time is proposed using the pedestrian starting time, the time interval between the pedestrian rows, and pedestrian crossing speed given area types (commercial, business, mixed, and elementary school areas), number of both-directional pedestrians for a cycle, crosswalk length and width.

• Journal of The Korea Institute of Healthcare Architecture
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• v.22 no.3
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• pp.17-26
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• 2016

Purpose: As the concerns for the environment are gradually prioritized, increasing interests of environment-friendly buildings are drawn. The Green Standard for Energy & Environmental Design (G-SEED) has been strengthened. However, there are no specific assessment criteria that reflect the special situation of healthcare facility. UK, US and Japan have green building certifications specially designed to evaluate sustainable healthcare facilities. This study has been started in order to provide basic information for developing assessment criteria for healthcare facilities in Korean Green Standard for Energy and Environmental Design. Methods: In this study, we investigated three foreign green building certifications and compared their assessment system and criteria for healthcare buildings. Results: Each of the three foreign certification standards showed the difference in the system, but all contained the contents specific to healthcare facilities. Evaluation items were affected by regional cultural environment and also medical environment. Patient safety and integrative planning were the most important assessment contents. Implications: Based on this analysis, Korean Green Standard for Energy & Environmental Design for healthcare facilities will be developed.