• Asian-Australasian Journal of Animal Sciences
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• 제31권1호
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• pp.63-70
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• 2018

Objective: The aim of the study was to isolate gossypol-degrading bacteria and to assess its potential for gossypol degradation. Methods: Rumen liquid was collected from fistulated cows grazing the experimental pasture. Approximately 1 mL of the rumen liquid was spread onto basal medium plates containing 2 g/L gossypol as the only source of carbon and was then cultured at $39^{\circ}C$ to isolate gossypol-degrading bacteria. The isolated colonies were cultured for 6 h and then their size and shape observed by microscope and scanning electron microscope. The 16S rRNA gene of isolated colonies was sequenced and aligned using National Center for Biotechnology Information-Basic Local Alignment Search Tool. The various fermentation conditions, initial pH, incubation temperature, inoculum level and fermentationperiod were analyzed in cottonseed meal (CSM). The crude protein (CP), total gossypol (TG), and free gossypol (FG) were determined in CSM after fermentation with isolated strain at $39^{\circ}C$ for 72 h. Results: Screening results showed that a single bacterial isolate, named Rumen Bacillus Subtilis (RBS), could use gossypol as a carbon source. The bacterium was identified by 16S rDNA sequencing as being 98% homologous to the sequence of Bacillus subtilis strain GH38. The optimum fermentation conditions were found to be 72 h, $39^{\circ}C$, pH 6.5, moisture 50%, inoculum level $10^7cell/g$. In the optimum fermentation conditions, the FG and TG content in fermented CSM decreased 78.86% and 49% relative to the control. The content of CP and the essential amino acids of the fermented CSM increased respectively, compared with the control. Conclusion: The isolation of a gossypol-degrading bacterium from the cow rumen is of great importance for gossypol biodegradation and may be a valuable potential source for gossypol-degradation of CSM.

• Asian-Australasian Journal of Animal Sciences
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• 제33권8호
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• pp.1242-1251
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• 2020

Objective: The 3×3 factorial arrangement was used to investigate if either high water-soluble carbohydrates (WSC) cultivars or suitable time of day that the grass cut could improve nutrient values and in vitro fermentation characteristics. Methods: The 3 cultivars were mowed at 3 diurnal time points and included a benchmark WSC ryegrass cultivar 'Premium', and 2 high WSC cultivars AberAvon and AberMagic, which contained, on average, 157, 173, and 193 g/kg dry matter (DM) of WSC, and 36.0, 36.5, and 34.1 g/kg DM of N during 7th regrowth stage, respectively. The fermentation jars were run at 39℃ with gas production recorded and sampled at 2, 5, 8, 11, 14, 17, 22, 28, 36, and 48 h. The rumen liquid was collected from 3 rumen fistulated cows grazing on ryegrass pasture. Results: High WSC cultivars had significantly greater WSC content, in vitro DM digestibility (IVDMD) and total gas production (TGP), and lower lag time than Premium cultivar. Methane production for AberMagic cultivar containing lower N concentration was marginally lower than that for AberAvon and Premium cultivars. Grass cut at Noon or PM contained greater WSC concentration, IVDMD and TGP, and lower N and neutral detergent fiber (NDF) contents, but CH₄ production was also increased, compared to grass cut in AM. Meanwhile, the effects of diurnal cutting time were influenced by cultivars, such as in vitro CH₄ production for AberMagic was not affected by cutting time. The IVDMD and gas production per unit of DM incubated were positively related to WSC concentration, WSC/N and WSC/NDF, respectively, and negatively related to N and NDF concentrations. Conclusion: These results imply either grass cut in Noon or PM or high WSC cultivars could improve nutrient values, IVDMD and in vitro TGP, and that AberMagic cultivar has a slightly lower CH₄ production compared to AberAvon and Premium. Further study is necessary to determine whether the increase of CH₄ production response incurred by shifting from AM cutting to Noon and/or PM cutting could be compensated for by high daily gain from increased WSC concentration and DM digestibility.

• 한국초지조사료학회지
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• 제11권4호
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• pp.244-251
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• 1991

초지의 유지관리용 2종 복합비료를 개발코자 경기화학(주)의 시제품(N-P$_2$O$_{5}$-K$_2$O-MgO-B$_2$O$_3$: 14-10-12-3-0.2)을 일반단비와 공시하여 겉뿌림 혼파초지에서 유지관리용 비료로서 비효를 구명하였다. 본 시험에서 토양화학성 및 무기염기의 상호균형(I보), 수량, 식생구성비율 및 목초의 영양성분(II보)에 미치는 영향을 검토하였다. 1. 시험후 토양화학성 변화를 보면 복비구가 단비구보다 Mg함량이 약간 높고 Ca함량은 낮은 경향을 보였으나 적정함량에는 모두 미달하였다. 초지적성등급수준과 비교하면 K함량을 제외하고는 CEC, Ca, Mg 수준이 매우 낮았다. 그러나 유효 P$_2$O$_{5}$ 함량은 단비 및 복비구의 보비수준에서 적정함량(200ppm)에 달하였다. 2. 토양중 치환성 Mg함량은 K보다 낮은 수준이었다. 복비보비구만 일반작물의 critical level(0.29 me Mg/100g)에 달하였으나 목초 및 방목우를 위한 critical level(0.41 me Mg/100g)에는 전시험구가 크게 미달하였다. 3. 염기포화도에서 Ca 및 Mg함량이 바람직한 수준에 크게 미흡하였으나 상대적으로 복비구는 Mg비가높았다. 또한 Mg/K, Ca/Mg, Ca : Mg : K당량비, %Mg of CEC는 바람직한 수준에 대체로 미흡하였으나 복비구가 단비구보다 상대적으로 양호한 특성을 보였다. 4. 본 토양화학성과 연관하여 볼때 초지조성시 석회일약토 비료(토양개량제)의 시용으로 Ca 및 Mg의 기본함량의 제고가 필요하였다.