• Horticultural Science & Technology
• /
• v.19 no.1
• /
• pp.54-59
• /
• 2001

The objective of this research was to develop a screening system for selection of grape downy mildew (Plasmopara viticola)-resistant grape cultivars or seedlings under greenhouse condition. Inoculum concentrations ranging from $10^4$ to $10^5spores/mL$ for screening of resistance were evaluated and $5{\times}10^4spores/mL$ was optimum. Of the tested 14 grape cultivars, Campbell Early and Niagara were resistant to grape downy mildew, Sheridan and Cheongsoo were moderate resistant, while Kaiji, Red Queen and Ruby Okuyama were susceptible under both greenhouse and field conditions. Vitis vinifera grape cultivars were more susceptible to grape downy mildew than V. vinifera-labrusca hybrids and V. vinifera-labrusca-aestivalis hybrids. In V. vinifera-labrusca hybrids, tetraploid grape cultivars were more susceptible than diploid culivars. The evaluated results of grape downy mildew resistance under greenhouse condition were in accordance with those of field condition. Results of this study indicated that both greenhouse and field procedures could be used to screen grape cultivars and seedlings for downy mildew resistance.

• Horticultural Science & Technology
• /
• v.33 no.4
• /
• pp.470-478
• /
• 2015

This study was investigated the anthocyanin composition of 'Gaeryangmeoru', 'Kyoho', and 'Hongisul' grapes cultivated in Korea using ultra-performance liquid chromatography (UPLC) coupled to a mass spectrometer (MS) equipped with an ESI (electrospray ionization) source. 'Gaeryangmeoru' is a dark-blue grape used for winemaking that can reach its coloring in unfavorable weather. The 'Kyoho' and 'Hongisul' varieties are hybrid grapes that feature black and pink skin, respectively. The anthocyanins extracted from the peels of grapes were analyzed using UPLC-ESI-MS/MS. Twenty-five anthocyanins were identified in the 'Gaeryangmeoru' and 'Kyoho' varieties, and 21 were identified in the 'Hongisul' variety. Eight, 14 and five predominant anthocyanins were identified in 'Gaeryangmeoru', 'Kyoho' and 'Hongisul' grape respectively. In all three varieties, mono-glucosides were 2.3-5.9 times more abundant than di-glucoside. Malvidin was the predominant anthocyanidin in 'Gaeryangmeoru' (44.1%) and 'Kyoho' (56.5%), but cyanidin (96.8%) was in 'Hongisul'. The acylated anthocyanins in 'Gaeryangmeoru' (2.0%) were rare, whereas acylated anthocyanins with p-coumaric acid were predominant in 'Kyoho' (40.9%) and 'Hongisul' (70.7%). In particular, cyanidin feruloyl glucoside was found only in the 'Hongisul' cultivar and considered to be useful as a criterion for identification of the variety. As a result, the grape varieties were demonstrated to have variety-specific anthocyanin characteristics, enabling classification based on anthocyanin composition in terms of anthocyanidins, glucosylation and acylation. The taxonomical application of anthocyanin composition confirmed the possibility that 'Gaeryangmeoru' originated from Vitis amurensis or its hybrids, and the 'Hongisul' grape was distinguished from other grapes by cyanidin feruloyl glucoside.

• Journal of agriculture & life science
• /
• v.52 no.6
• /
• pp.49-60
• /
• 2018

Here, we reported 159 varieties of major cultivars using grapevine genetic resources to identify the resistant grape ripe rot cultivars. To do this, we performed pathogenicity assays from these grape cultivars by inoculating Colletotrichum acutatum and Colletotrichum gloeosporioides. Genotyping-by-sequencing(GBS) method was also used to compare genetic diversity among grape varieties. As a result, leaves inoculated with C. acutatum showed that 58 cultivars were susceptible, while 17 cultivars were resistant. In the case of C. gloeosporioides, 34 cultivars were found to be susceptible, while 25 cultivars were resistant. The 8 cultivars that showed resistance to both species were 'Agawan', 'Huangguan', 'Xiangfei', and 5 other cultivars from the hybrids of European and American species. Most of the varieties such as 'Emerald Seedless', 'Tano Red', and 'Rem 46-77(Aestivalis GVIT 0970)' originated in European species were identified as susceptible. These results can be used in the effective management of grape disease. In addition, these findings provide information for the development and cultivation of resistant to grape ripe rot disease cultivars.