• Title/Summary/Keyword: granulosis virus

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Maturation of the Granulosis Virus of Common Cabbage Worm, Pieris rapae (배추흰나비 과립병(顆粒病)바이러스 감염조직(感染組職)에서의 바이러스 성숙(成熟))

  • Ryu, Kang-Sun;Kang, Seok-Kwon
    • Applied Microscopy
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    • v.21 no.1
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    • pp.77-85
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    • 1991
  • The virus maturation in fat body cells infected with P. rapae granulosis virus were also examined to have the following results. Thin section of P. rapae GV granules showed a regular lattice of the granule enclosing virus particles. Virus particles were observed to penetrate the fat body cells by fusion or viropexis type. The blood and fat body cells uptaked the granulosis viruses a phagosome, in which they were digested by lysosomal enzymes.

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Biochemical Characteristics of the Granulosis Viruses DNA of Common Cabbage Worm, Pieris rapae and Pieris brassicae (배추흰나비 과립병바이러스 DNA의 생화학적 특성)

  • 류강선;진병래;강석권
    • Korean journal of applied entomology
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    • v.30 no.2
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    • pp.138-143
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    • 1991
  • This study was carried out to acquire some basic biochemical informations on the granulosis virus (GV) DNA of Pieris rapae and Pieris brassicae. The thermal denaturation temperature (Tm) and G+C content of the DNA of the viruses were $83.7^{\circ}C$ and 35.5% for P. rapae GV, $84.0^{\circ}C$ and 35.9% for P. brassicae GV, respectively. There were some differences in the DNA fragmentation patterns of the two GV's produced by digestion with restriction endonucleases such as EcoR I , BamH I and Hind m . The homololgy between the two DNAs was caculated to be 97.0%. The size of the genome was estimated to be 103 kbp for P. rapae GV and 108 kbp for P. brassicae GV.

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Biochemical Characteristics of the Granulosis Viruses Proteins of Common Cabbage Worm, Pieris rapae and Pieris brassicae (배추흰나비 과립병바이러스 단백질의 생화학적 특성)

  • 류강선;진병래;강석권
    • Korean journal of applied entomology
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    • v.30 no.3
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    • pp.180-186
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    • 1991
  • This study was carried out to acquire some basic biochemical informations on the granulosis virus(GV) of Pieris rapae and Pieris brassicae. The capsule protein was composed of a single polypeptide with a molecular weight of 30,000 dalton for P. rapae GV and 31,000 dalton for P. brassicae GV. The major amino acids of capsule protein were glutamic acid, aspartic acid and lysine. When the capsule protein was partially digested with trypsin, chymotrypsin, papain or Staphylococcus aureus V8 protease, the digested products of the two viruses showed no difference in electrophoretic mobility. The patterns of the polypeptides of the two virus particle on SOS-polyacrylamide gel showed a little difference in high molecular weight region(over MW 100 kd).

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Purification and Pathogenicity of the Granulosis Viruses of Common Cabbage Worm, Pieris rapae and Pieris brassicae (배추흰나비 과립병바이러스의 정제 및 병원성)

  • 류강선;김근영;강석원
    • Korean journal of applied entomology
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    • v.30 no.3
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    • pp.187-195
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    • 1991
  • This study was carried out to acquire some basic biological informations on the granulosis virus (GV) of Pieris rapae and Pieris brassicae. Purified fractions of GV capsules in an sucrose density gradient centrifugation yielded on homogenous and sharp peak without a shoulder. Electron microscopy revealed that GV capsules were mostly ovalglove in shape. P. rapae and P. rapae GV isolated from P. rapae comprised granules($396\pm38\times238\pm25nm$ for P. rapae GV. $375\pm40\times255\pm28nm$ for P. brassicae GV) which contained single virus particle. The virus particles were 250- $275{\times}63$ -73nm for P. rapae GV and 243-250 $\times$ 63-75nm for P. brassicae GV containing a nucleocapsid 225 $\times$ 31nm for P. rapae, 225 $\times$ 29nm for P. brassicae within an envelope. The virulent difference between the two viruses was very small in their virulence for P. rapae larvae showing the $LC_{50}$( -log) with 5.5673 for P. rapae GV and 5.8104 for P. brassicae GV. Also the $LT_{50}$ of the 3rd instar P. rapae larvae against $10^{-6}$ inoculum was 8.17 days for P. rapae GV and 7.16 days for P. brassicae GV.

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