• BMB Reports
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• v.51 no.5
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• pp.236-241
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• 2018

Anoctamin 1 (ANO1) is an anion channel that is activated by changes in cytosolic $Ca^{2+}$ concentration and noxious heat. Although the critical roles of ANO1 have been elucidated in various cell types, the control of its gating mechanisms by $Ca^{2+}$ and heat remain more elusive. To investigate critical amino acid residues for modulation of $Ca^{2+}$ and heat sensing, we constructed a randomized mutant library for ANO1. Among 695 random mutants, reduced $Ca^{2+}$ sensitivity was observed in two mutants (mutant 84 and 87). Consequently, the E143A mutant showed reduced sensitivity to $Ca^{2+}$ but not to high temperatures, whereas the E705V mutant exhibited reduced sensitivity to both $Ca^{2+}$ and noxious heat. These results suggest that the glutamic acids (E) at 143 and 705 residues in ANO1 are critical for modulation of $Ca^{2+}$ and/or heat responses. Furthermore, these findings help to provide a better understanding of the $Ca^{2+}$-mediated activation and heat-sensing mechanism of ANO1.

• Parasites, Hosts and Diseases
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• v.54 no.6
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• pp.751-758
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• 2016

This study aimed at constructing a draft genome of the adult female worm Toxocara canis using next-generation sequencing (NGS) and de novo assembly, as well as to find new genes after annotation using functional genomics tools. Using an NGS machine, we produced DNA read data of T. canis. The de novo assembly of the read data was performed using SOAPdenovo. RNA read data were assembled using Trinity. Structural annotation, homology search, functional annotation, classification of protein domains, and KEGG pathway analysis were carried out. Besides them, recently developed tools such as MAKER, PASA, Evidence Modeler, and Blast2GO were used. The scaffold DNA was obtained, the N50 was 108,950 bp, and the overall length was 341,776,187 bp. The N50 of the transcriptome was 940 bp, and its length was 53,046,952 bp. The GC content of the entire genome was 39.3%. The total number of genes was 20,178, and the total number of protein sequences was 22,358. Of the 22,358 protein sequences, 4,992 were newly observed in T. canis. Following proteins previously unknown were found: E3 ubiquitin-protein ligase cbl-b and antigen T-cell receptor, zeta chain for T-cell and B-cell regulation; endoprotease bli-4 for cuticle metabolism; mucin 12Ea and polymorphic mucin variant C6/1/40r2.1 for mucin production; tropomodulin-family protein and ryanodine receptor calcium release channels for muscle movement. We were able to find new hypothetical polypeptides sequences unique to T. canis, and the findings of this study are capable of serving as a basis for extending our biological understanding of T. canis.

• Korean Journal of Human Ecology
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• v.9 no.3
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• pp.5-21
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• 2006

The purpose of this study was to grasp the consciousness of adolescents about their future in old aged housing. This research was executed with a questionnaire survey method. Data samples were collected from 255 students who finished first grade curriculum in high schools in Chonbuk province and Seoul city. The results were as followed. First, a healthcare plan was very highly recognized by students, and they wanted to live as couples or single in their old age. Second, the respondent students recognized elderly housing as a home for the aged, a silver town and nursing home. 72.1% of the respondents preferred a single detached house as an elderly house form. They preferred an elderly housing district with well provided convenient facilities for daily living. They wanted to live in the country or rest areas for the aged. They preferred an interior space composition with LDK with 2 bedrooms. 52.0% students wanted a neighborhood for their friends to live together. Third, 28.3% of the respondent students thought the appropriate time to move in elderly exclusive housing was 66-70 years old. And when their physical condition worsened or they needed care, they would. want to move into that housing. Also, they wanted the most convenient facility and a medical care facility in elderly exclusive housing. Forth, students' space design needs for elderly were high, more than 4 points. It was especially needed safety in space design, and this grade was 4.56(,53) points. Also the needs of safety and the convenient facilities.equipments were statistically significant by boys and girls.

• Journal of Korean society of Dental Hygiene
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• v.13 no.6
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• pp.1047-1055
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• 2013

Objectives : The purpose of this study was to evaluate the overall oral health knowledge and toothbrushing pattern of school dental clinic students in school dental clinics and to provide the basic data for the oral health. Methods : The subjects were 290 elementary school students visiting school dental clinics in Busan Metropolitan city. The data were collected from July 3, 2012 to July 19, 2013. Results : The visit rate of school dental clinics was 40.3%. Girl students brushing three times a day accounted for 47.3% and boy students brushing twice a day accounted for 58.5%(p<0.05). Students using rolling brushing method accounted for 35.4% and 8% of the students did not know the rolling method. Brushing method response consisted of circular motion(21.6%) and rolling method(30.8%). Brushing more than 3 minutes accounted for 46.5%(p<0.001) and 30.8% of the students chose rotation brushing method(p<0.001). Conclusions : It is very important for the elementary school students to learn the right oral health education and oral health knowledge.

• Health Policy and Management
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• v.18 no.1
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• pp.63-84
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• 2008

The aim of this study was to analyze the correlation between professional autonomy and ideology among Korean physicians and to investigate how these factors affect job satisfaction like social status satisfaction and economic reward satisfaction. This study utilized a self-administered questionnaire survey and collected data nationwide between July and August, 2003. 211 responses were used for final analysis. SPSS 12.0 was used for a chi-square test, one-way ANOVA, Pearson correlation analysis, independent t-test and hierarchical multiple regression analysis. The results of this study were as follows. First, many variables of ideological factor were related to job satisfaction. Second, physicians expecting the change of political influence has patient-centered attitude. Third, there were many relationships between professional autonomy and ideology variables. Fourth, physicians expecting the change of political influence and customer-centered healthcare system showed more job satisfaction. In conclusion, professional autonomy is related to ideology, and in order to enhance job satisfaction, ideological factor needs more development. In addition, market-oriented healthcare system would contribute to enhance the job satisfaction of physicians expecting the change of political influence and customer-centered healthcare system.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.11 no.5
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• pp.1929-1935
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• 2010

Approximately 110 elementary schools and 400 teachers in Dae Jeon metropolitan city were chosen for this research, by using the method of direct visit and survey by mail. The survey was performed as in the forms of self administration, by simple statistic extract method, and among the survey, insincere answers and partly omitted contents were removed, which left 348 survey questionnaires to use for the final research. In order to restore the tap water fluorine concentration regulation project in Dae Jeon, advertise its benefits; it offered precise knowledge and positive response of fluorine through education on oral disease prevention, provide correct knowledge, administrative support should follow to support its importance.

• Korean Journal of Community Nutrition
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• v.17 no.6
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• pp.782-794
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• 2012

The purpose of this study was to investigate the Korea-resident foreigners's adaptability and preference to Korean foods. The survey was carried out among 144 foreigners living in Seoul, Korea (male 57, female 87; from the East 109, from the West 35). Based on the first impression of Korean food, 'spicy', 'strong taste of seasoning', 'salty' were most common. About 90 percent of the foreigners adapted to Korean food in six months. It took more time to adapt to Korean food for Western people, compared to people from the East. Factors that influenced their adaption to Korean food were shown to be 'efforts by myself' and 'from friends'. Foreigners posited positive attitude toward Korean food according to their answers like 'nutritionally great food' and 'food with interesting ways of eating'. Westerners appeared to be more satisfied with Korean food. 'Too strong seasoning taste' and 'too sweet' were pointed out for further improvements. Beef Bulgogi, (Korean) fruit, Beef Ribs, Pork Ribs, and Grilled Pork Belly in order were foreigners' favorite foods, but Soju, Korean Sausage, Sliced Rice Cake Soup, Radish Kimchi and Vegetable Side Dishes were not. Taken together, the adaptability and preference to Korean foods to foreigners were different according to the gender and cultural background. Target marketing strategy of Korean Foods should be considered for foreign customers.

• Korea Journal of Hospital Management
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• v.20 no.3
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• pp.13-23
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• 2015

This research is intended to inquire any discrepancy depending on the variables such as types, geography and size of hospitals of Korea in order to develop differentiated marketing strategy and to investigate how the aforementioned variables affect the management performance such as the increase in the number of foreign patients, their willingness to re-visit, the increase in profit and etc. The survey for this research was conducted for five weeks from July 10th 2014 to August 30th 2014 with 251 participants in charge of foreign patient attraction for 161 domestic hospitals. As the method of the research, a hypothesis was first established based on previous studies, followed by the incorporation of self-administered questionnaire to confirm the formulated hypothesis. Frequency analysis and ANOVA analysis were used to analyze the result of the survey. The outcome of the research and the implications are as follows. First, according to the demography of the persons-in-charge of foreign patient attraction, the proportion of female was superbly high by 76.5%. As for the age, those in their 30s were highest in proportion. Finally, for the education level, college graduates took up the largest portion by 46.5%. Second, in respect to the hypothesis assuming the difference in performance depending on the types of hospitals, national university-affiliated hospitals showed the highest level of the increase in foreign patients by the average of 3.25. Third, in respect to the hypothesis assuming the difference in performance based on geographical position, it was confirmed that hospitals in Ulsan City experienced the largest growth in the number in foreign patients. Fourth, in respect to the sizes of hospitals, those with 201 to 300 sickbeds showed the highest increase by the average of 3.45. The implication of the above research outcome indicates that while the number of foreign patients visiting Korea for medical purposes is on the rise, the number of professionals in place with necessary knowledge and capacity is insufficient and requires improvement.

• Journal of Digital Convergence
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• v.14 no.8
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• pp.497-502
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• 2016

The survey is designed to provide fundamental data for assessing effects of calligraphy therapy program. An opened questionnaire asking psychological and physical experiences (3 advantages and 3 disadvantages) was given to 150 persons practicing calligraphy. Collected data were categorized into 10 psychological advantages (435 items), 6 psychological disadvantages (129 items), 9 physical advantages (302 items), and 7 physical disadvantages (150 items). Those subjective experiences were multidisciplinarily interpreted in terms of the mental and physical health and the humanistic education. A new structured assessing tool which will be developed based on the findings from the study may contribute in activating the calligraphy therapy program.

• Quality Improvement in Health Care
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• v.15 no.2
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• pp.107-120
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• 2009

Background : To estimate fall incidence rate and associated factors in inpatients from a general hospital. Method : The data were collected from 104 fall incident reports developed by the patient safety committee in a general hospital in Seoul from 01 January 2007 to 31 December 2008. Information included general characteristics of patients, factors related to fall, types, places, circumstances and outcomes of fall. Result : The incidence rate of fall, which was 4.4 per 1,000 total discharged patients and 0.5 per 1,000 patient-days, was much lower than that of several hospitals in the United States. The difference may reflect the different incidence reporting system of each hospital. Fall-prone patients were, in general, $$\geq_-$$65 years of age, had an alert mental status, were ambulatory with some assistance, and were dependent on and ambulatory device. High incidence of falls was associated with patients with circulatory disease. The majority of fall events usually occurred in bed or at the bedside in the patient's room, and occurred more often during the night than during the day or evening. Risk factors of fall were use of drugs (antihypertensive or neuropsychiatric drugs) and environmental factors (e.g., overly high bed height, surrounding objects, inadequate fitness shoes and slippery floor). Physical injury occurred in 43.3% of fall events, which typically required diagnosis of injury and treatment such as suturing. Risk factors for repeated falls were use of a neuropsychiatric drug (odds ratio=13.9) and gait disturbance (odds ratio=91.2). Risk factors for fall-related injury were alert mental status (odds ratio=3.3 times more likely to fall than those who were drowsy or in a stupor) and general weakness(odds ratio=3.3 times more likely to fall than those who were not generally weak). Conclusion : Medical and nursing staff should be aware of the fall risk factors of hospitalized patients and should intensively pursue preventative strategies. Development of fall prevention education based on these results is recommended.