• Molecules and Cells
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• 제20권2호
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• pp.173-182
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• 2005

Heat shock protein gp96 is an endoplasmic reticulum chaperone, belonging to the HSP90 family. The function of gp96 as a molecular chaperone was discovered more than 10 years ago, but its importance has been overshadowed by the brilliance of its role in immune responses. It is now clear that gp96 is instrumental in the initiation of both the innate and adaptive immunity. Recently, the roles of gp96 in protein homeostasis, as well as in cell differentiation and development, are beginning to draw more attention due to rapid development in the structural study of HSP90 and some surprising new discoveries from genetic studies of gp96. In this review, we focus on the aspect of gp96 as an ER molecular chaperone in protein maturation, peptide binding and the regulation of its activity.

• 한국어병학회지
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• 제23권3호
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• pp.409-420
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• 2010

Effects of various concentrations of garlic powder and garlic extract in the diets on growth, serum chemistry and immune response of olive flounder were determined. Thirty-five juvenile fish averaging 5.1 g were randomly distributed into 21 of 180 L flow-through tanks. Seven experimental diets with various concentrations of garlic powder (GP) and garlic extract (GE) were prepared in triplicate: GP-0 without garlic supplementation, GP-0.5, GP-1, GP-2, GP-3 and GP-5 diets containing garlic powder at the concentrations of 0.5, 1, 2, 3 and 5%, respectively at the expense of wheat flour and finally, GE-0.4 diet containing 0.4% garlic extract were prepared. At the end of the 8-week feeding trial, serum chemistry of fish was measured. In addition, twenty fish from each tank were artificially infected with E. tarda for the following 96 h to monitor cumulative mortality. Weight gain of fish fed GP-0 diet was higher than that of fish fed GP-1, GP-2, GP-3 and GP-5 diets. No difference in serum criteria (total protein, glucose, glutamate oxaloacetate transaminase, cholesterol and triglyceride levels) of olive flounder was found among the experimental diets except for glutamate pyruvate transaminase. Lysozyme activity of fish fed GP-0, GP-1, GP-3 and GE-0.4 diets was higher than that of fish fed GP-5 diet. The highest cumulative mortality was 93.3% in fish fed GP-0 diet at 96 h after E. tarda infection, followed by GP-3, GP-1, GP-5, GP-2, GP-0.5 and GE-0.4 diets. In considering these results, dietary inclusion of garlic powder and garlic extract has no distinctive positive effect on improvement in growth, serum chemistry and immune response of olive flounder in this experimental conditions, therefore, its application should be carefully considered.

• 한국환경생태학회지
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• 제12권3호
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• pp.259-270
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• 1998

본 연구는 산지의 수자원보전량 평가용 수문유출모형의 개발을 위한 중요한 기초연구로서 우리나라 주요 수종인 소나무와 상수리나무에 대한 수간류 및 수관통과우의 물수지식과 수문학적 특성을 밝히고자 수행하였다. 영남대학교 자연자원대학 부속 용성연습림과 영남대학교 교내 야산에 각각 수관통과우 및 수간류 관측용 야외 실험지를 설치하고, 1995년 9월에서 1998년 6월까지 약 2년 10개월 사이에 이 지역에 내린 다수의 단위강우에 대한 두 수종의 임내우 수량배분을 조사하였다. 그 결과 임외의 단위강우에 응답하는 두 수종의 수문학적 특성과 임내우 물수지식을 요약하면 다음과 같다. 1. 소나무에 있어서 수관통과우와 수간류의 총량은 각각 임외우의 73.8%와 0.8%이였으며 상수리나무는 각각 임외우의 76.9%와 3.8%로 관측되었다. 2. 소나무에 있어서 임외우(Gp)에 대한 수간류(S$_{f}$)와 수관통과우(T$_{f}$)의 회귀식은 각각 S$_{f}$ = 0.01GP -2.05 ($r^2$=0.54) T$_{f}$ = 0.79Gp - 26.04 ($r^2$=0.92)로, 임내우(N$_{r}$)와는 N$_{r}$ = 0.81Gp - 28.09 (r2=0.92)로 추정하였다. 이와 같이 소나무에 있어서 수간류와 수관통과우는 임외우의 양에 정비례하여 증가하였다. 3. 상수리나무에 있어서 임외우(Gp)에 대한 수간류(S$_{f}$)와 수관통과우(T$_{f}$)의 회귀식은 각각 S$_{f}$ = 0.03Gp + 12.25 ($r^2$=0.74) T$_{f}$ = 0.78Gp + 19.75 ($r^2$=0.96)로, 임내우(N$_{r}$)와는 N$_{r}$ = 0.81Gp + 31.99 ($r^2$=0.96)로 구해졌는데 소나무보다 상수리나무에 있어서 수간류와 수관통과우의 증가폭이 더 큰 것으로 나타났다. 4. 수간류 및 수관통과우와 그 영향인자로 평가된 수관투영면적(CA). 흉고직경(DBH), 임외우량(Gp)과의 내부상관분석을 한 결과, 소나무 수간류에는 임외우＞수관투영면적＞흉고직경의 순으로, 상수리나무 수간류에는 임외우＞흉고직경＞수관투영면적의 순으로 상관계수 값이 높았다. 그리고 소나무와 상수리나무의 수관통과우는 임외우＞수관투영면적＞흉고직경의 순으로 상관계수 값이 높았다.상관계수 값이 높았다.

• 한국측량학회지
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• 제17권1호
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• pp.33-39
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• 1999

정보화 시대를 맞이하여 정확하고 신속한 지형정보의 취득이 점차로 중요해짐에 따라서 현재 GPS를 이용하여 삼차원의 측지정보를 취득하기 위한 연구가 활발히 진행되고 있다. 그러나 현재 실용적으로 사용되고 있는 높이정보의 기준면은 물리적인 특성을 가지는 지오이드면에 기초하기 때문에 기하학적인 특성이 강조되는 GPS로부터 취득되는 높이정보는 그 정확도가 실용적으로 미흡하다는 평가를 받고 있다. 본 연구에서는 이를 개선하기 위하여 미국 국방부에서 공개한 지오이드 모델인 EGM96 모델로부터 반복계산법에 의하여 베셀 지오이드고를 계산하고 이를 우리나라 1등 삼각점에 대하여 측정이 이루어진 36점의 GPS성과와 실용성과를 사용하여 좌표변환 매개변수를 계산하여 이의 변환정확도를 평가하였으며, 또한 56점의 수준점에 대한 GPS성과를 좌표변환과 GPS/Leveling을 통하여 계산한 정표고를 수준점 성과와 비교하여 EGM96 모델로부터 계산된 Bessel 지오이드고의 실용성을 평가하였다.

• 한국농공학회지
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• 제41권6호
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• pp.90-96
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• 1999

Over 4.1 billion metric tons of non-hazardous solid wstes are generated in the United States annually. Therefore, there is a need to reuse or reprocess as many products or by-products as possible. In this report, theaggregates produced from Naval Shipyard and Charleston area dredge spoils are designated as GP-21-23 and GP 4-5, repectively. The major objective of this study is to determine the feasiblity of the use of these aggregates in concrete. The followings are some of the conclusions, observations and recommendations regarding this portion of the research project. In most cases, the unit weight of concrete containing GP 21-23 and GP 4-5 (vitrified galss) lower than the control concrete, and the concrete containing vitrified glass produced lower compressive and bending strength compared to the control. However the concrete containing virtrified glass could be used in the field based on the minimum criterial set for the mix design. The use of vitrified glass in concrete is recommended in several applications except parking lots, side walks and main roads, because abrasion resistace is not high.

• Pediatric Gastroenterology, Hepatology & Nutrition
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• 제22권5호
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• pp.431-440
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• 2019

Purpose: No national survey has yet described the guidelines followed by Korean pediatricians to treat acute gastroenteritis (AGE). An online survey was performed to investigate the management of AGE followed by members of The Korean Society of Pediatric Gastroenterology, Hepatology, and Nutrition, and the results were compared between pediatric gastroenterologists (PG) and general pediatricians (GP). Methods: Questionnaires were sent to pediatricians between June 2 and 4, 2018 regarding the type of hospital, indications for admission, antiemetic and antidiarrheal drugs and antibiotics prescribed, and dietary changes advised. Results: Among the 400 pediatricians approached, 141 pediatricians (35.3%) responded to the survey. PG comprised 39% of the respondents and 72.7% worked at a tertiary hospital. Both PG and GP considered diarrhea or vomiting to be the primary symptom. The most common indication for hospitalization was severe dehydration (98.8%). Most pediatricians managed dehydration with intravenous fluid infusions (PG 98.2%, GP 92.9%). Antiemetics were prescribed by 87.3% of PG and 96.6% of GP. Probiotics to manage diarrhea were prescribed by 89.1% of PG and 100.0% of GP. Antibiotics were used in children with blood in diarrheal stool or high fever. Dietary changes were more commonly recommended by GP (59.3%) than by PG (27.3%) (p<0.05). Tests to identify etiological agents were performed primarily in hospitalized children. Conclusion: This survey assessing the management of pediatric AGE showed that the indications for admission and rehydration were similar between GP and PG. Drug prescriptions for diarrhea and dietary changes were slightly commonly recommended by GP than by PG.

• 대한전기학회:학술대회논문집
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• 대한전기학회 2007년도 심포지엄 논문집 정보 및 제어부문
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• pp.95-96
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• 2007

GPS를 사용할 경우 반송파 위상 측정치를 이용한 정밀한 위치 측정이 가능하다. 그러나 GPS만을 사용할 경우, 미지정수 결정 성공률이 낮으며, 도심과 같은 곳에서 신호를 수신하지 못하는 경우가 발생한다. 이 문제는 의사위성을 추가하여 해결할 수 있다. 본 논문에서는 GPS에 의사위성을 추가하여 미지정수의 결정 성공률이 향상되고 가용성을 향상시킬 수 있으며 정확도가 향상됨을 보였다.

• 농촌계획
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• 제26권3호
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• pp.91-102
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• 2020

The purpose of study was analyze the pumping characteristics, Irrigation Efficiency(IE), and irrigation pattern by period of rice growing stage with based on the performance of design irrigation water requirement and operational Galshin Pumping(GP) station in GP irrigation district constructed under rural water development project master plan. GP station was located in Yedang reservoir, Yesan-gun, Chungcheongnam-do and has been supplying irrigation water since 2006. The research data are the Irrigation Water Requirement(IWR) and the Pumping Water Amount(PWA) from 2006 to 2015 at the GP station, which is the supplied amount. The IWR were calculated using the Blaney-Criddle formula of the HOMWRS program, Hydrological Operation Model for Water Resource System, developed by Korea Rural Community Corporation. The Blaney-Criddle formula was used to calculate design irrigation water requirement of Galshin rural water development project master plan. During 2006-2015, the study period, the annual average IWR is 763.2(±149.1)mm, the annual PWA of the GP station is 397.4mm to 1,056.9mm, and those average annual PWA is 643.4(±208.4)mm. The annual IE of GP station 96.5% to 169.0%, and the average annual IE is 124.3%, which is higher than the research results conducted in other pumping stations. Analyzing the irrigation patterns of the GP irrigation district, the IWR Ratio per 10days(IWRR) and the PWA Ratio per 10days(PWAR) of the G P station were obtained. The IWRR is the percentage of IWR for each 10 days of a month to total IWR per year, and the PWAR is the percentage of PWA for each 10 days of a month to total PWA per year. The Kolmogorov- Smirnov(K-S) test results of IWRR and PWAR showed the characteristics classification by rice growing stage and stable normal distribution characteristics. Average IWRR(AIWRR) and Average PWAR(APWAR) are presented as irrigation patterns. Irrigation pattern analysis will be able to standardize comparison, analysis and probability calculation of the pumping station characteristics of different pumping stations and apply to objective evaluation of the pumping station district.

• Asian Pacific Journal of Cancer Prevention
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• 제15권4호
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• pp.1639-1642
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• 2014

The multidrug resistance phenotype is one of the major problems in development of cancer cell resistance to chemotherapy. Some natural compounds from medicinal plants have demonstrated promising capacity in enhancing anticancer effects in drug resistant cancer cells. We aimed to investigate whether mangiferin might have an ability to re-sensitize MCF-7 breast cancer cells previously treated with short-term doxorubicin in vitro, through the modulation of efflux transporters, P-glycoprotein (P-gp), MRP1 and BCRP. We exposed MCF-7 breast cancer cells pretreated with doxorubicin for 10 days to mangiferin (10, 25 or 50 ${\mu}M$) for 96 hours. Afterwards, we evaluated influence on cell viability and level of mRNA expression of P-gp, MRP1 and BCRP. Doxorubicin given in combination with mangiferin at low concentrations (10 and 25 ${\mu}M$) failed to give significant reduction in cell viability, while at the highest concentrations, the combination significantly reduced cell viability. The mRNA expression analysis of P-gp, MRP1 and BCRP showed that mangiferin had inhibitory effects on P-gp but no effects on MRP1 and BCRP. In conclusion, we suggest that mangiferin at high concentrations can be used as chemosensitizer for doxorubicin therapy. This effect might be attributed by inhibitory effects of mangiferin on P-glycoprotein expression.

• 한국자원식물학회:학술대회논문집
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• 한국자원식물학회 2018년도 추계학술대회
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• pp.87-87
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• 2018

This study was conducted to investigate the effects of osmotic priming, liquid smoke (LS), sonication and modified drum priming treatments to improve the germination speed and uniformity of pepper seeds for high quality export seeds. Seeds were treated in 0.5, 1.0, 5.0, 10% LS solution only or with 100mM $K_2SO_4$ solution for 6days at $15^{\circ}C$. Sonication treatment was performed for 5, 10, and 20 minutes at an intensity of 5.2, 10.4, 15.7, 21.0, 26.1kHz in water at $15^{\circ}C$. After sonication treatment, seeds were primed with water or 100mM $K_2SO_4$ for 4days. 40, 50 and 60% seed moisture content (SMC) of hydrated seeds were incubated for 84, 96 and 108h in a container with a relative humidity of 99% at 26rpm for a modified drum priming treatments. 0.5% LS treatment with water or 100mM $K_2SO_4$ priming showed significant effects with 71% GP (9.0days MGT) and 66% GP (7.4days MGT), respective, while untreated seeds resulted in only 61% GP and 10days MGT. The healthy seed % was increased by 24% by $K_2SO_4$ priming with LS treatment compared to untreated seeds. However, sonication treatment showed less germination or no difference compared to untreated seed, regardless of intensity and treatment time. The modified drum priming treatment (108h incubation after 50% SMC hydration) significantly improved the GP (on the $4^{th}$days after sowing), germination rate (GR), and MGT to 65%, $28%{\cdot}day^{-1}$, and 3.6days, whereas untreated seed resulted only 7% GP, $17%{\cdot}day^{-1}$ GR and 5.9days MGT. Thus, modified drum priming treatment effectively improved seed germination and is considered as an industrially promising treatment methods considering the shortening of the treatment period and environment-friendly aspects.