• Korean Journal of Ecology and Environment
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• v.54 no.2
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• pp.120-124
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• 2021

In order to understand the vegetative role of Glycine soja, we studied the basic physiological characteristics between Glycine soja and Glycine max. For this study, the light intensity (μmol m^-2 s^-1) on leaf surface, leaf temperature (℃), transpiration rate (mmol m^-2 s^-1), photosynthetic rate (μmol m^-2 s^-1), substomatal CO₂ partial pressure (vpm) of Glycine soja and Glycine max were measured, and the quantum yield, photosynthesis rate per substomatal CO₂ partial pressure were calculated. In the results of simple regression analysis, the increasing quantum yield decreases leaf temperature both of Glycine soja and Glycine max and the increasing leaf temperature decreases transpiration rate in case of Glycine soja. However, in case of Glycine max, the increasing leaf temperature decreases substomatal CO₂ partial pressure, photosynthetic rate, and photosynthetic rate per substomatal CO₂ partial pressure as well as transpiration rate. Also, increasing transpiration rate increases substomatal CO₂ partial pressure while decreases photosynthetic rate per substomatal CO₂ partial pressure. Thus, Glycine soja is relatively more easily adaptable to severe environments with low soil nutrients and high light levels. Compared to Glycine max susceptible to water loss due to a water-poor terrestrial habitat, the physiological traits of Glycine soja has a high average transpiration rate and are less susceptible to water loss will act as a factor that limits the habitat according to soil moisture.

• 대한상한금궤의학회지
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• v.4 no.1
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• pp.29-36
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• 2012

Aim : To investigate the hepatotective effect of Gardenia jsaminodes and Glycine max aqueous extract against D-galactosamine (d-GalN, 300mg/kg body weight) was administered to the male Sprague Dawley (SD) rats. Materials and Methods : The study was carried out on male SD rats (age matched, weight $250{\pm}10g$). Experimental groups divided four: Normal group (Nor) was administered saline, Control (Con) group was administered saline after d-GalN treatment. Experimental group (Exp) was administered Gardenia jsaminodes (200 mg/kg; Ga group), Glycine max (700 mg/kg; Gl group), and Gardenia jsaminodes+Glycine max (200 mg/kg+700 mg/kg, GG group) during 14 days(n=5). Results : d-GalN administration induced hepatotoxicity in rats which was manifested by increased levels of alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase but decreased total cholesterol and triglyceride. Treatment with Gardenia jsaminodes extract significantly protected the liver in d-GalN administered rats. Conclusion : Gardenia jsaminodes aqueous extract and Gardenia jsaminodes+Glycine max extract possesses hepatoprotective potential, thus validating its use in alleviating toxic effects of d-GalN.

• Journal of Environmental Health Sciences
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• v.23 no.1
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• pp.50-54
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• 1997

The removal efficiency of nutrient was investicated by using Glycine max Meer seedling. After budding, Glycine max Merr was raised at darkness for 4 days. During cultivation, the removal efficiency of $NO_2-N+NO_3-N$ was up to 90% with initial concentration of 20-100 ppm. The removal efficiency of PO$_4$-P was up to 80% with initial concentration at 30 ppm, but it was down to 22% and 27% at 40 ppm and 50 ppm. When the removal efficiency of nutrient was compared with alternating 12 hours' light and darkness, the removal efficiency of NO$_2$-N + NO$_3$-N was up to 90% at below 60 ppm. It was not different from each other. But it was particularly low about 62% and 34% at 80 ppm and 100 ppm in alternating 12 hours' light. The removal efficiency of PO$_4$-P was low at alternating 12 hours' light between 10-50 ppm on the whole range. The neutralizing capacity of pH was shown in acidity and alkalinity except strong acidity(below pH 3). The initial pH was neutralized at 6.0-7.7 of pH after 4 days. Particularly, Glycine max Meer seedling that was difference from other water plants, was shown the neutralizing capacity in strong alkalinity.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.41 no.5
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• pp.608-618
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• 1996

Wild perennial types of genus Glycine, as sources of genetic diversity, have been recently employed in the soybean breeding programs. This study was carried out to introduce some useful characters of wild perennial Glycine tomentella into common cultivars, G. max. Parent materials used for interspecific hybridization were three common cultivars, Ulsankong, Baemkong and Danyeobkong, and four G. tomentella strains having different diploid(2n) of 38, 40, 78, and 80. Their cross-fertility, effect of reciprocal cross, development comparison of crossing pod and optimum timing to efficient crossing were investigated. Plant were grown in 1/2,000 Wagner pots in greenhouse and field. Crossing efficiency and pod survival rate were the highest in 2n=38 of the four G. tomentella strains when the common cultivars were fixed as female parents. In the reciprocal cross of three common cultivars and four G. tomentella strains, when the former was used as maternal sources, they showed better crossing rate and longer pod survival than the latter. In the interspecific hybridization between G. max cv. Baemkong andG. tomentella, 2n=38, the pod formed from the female G. tomentella had relatively greater size and weight than that from the female Baemkong in comparison with parents. However, the reverse result was true in a number of ovules per pod. More efficient interspecific hybridization in the field was made on August 20, when temperature and relative humidity were higher and day length was shorter, than one or two months earlier.

• Preventive Nutrition and Food Science
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• v.6 no.1
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• pp.38-42
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• 2001

The methanol extracts of 25 leguminous seeds in vitro were evaluated for inhibitory activities against lens aldose reductase of Sprague Dawley male rats. The responses varied with both leguminous seed and concentration used. At the concentration of 0.1 mg/mL, the methanol extracts from Amphicaraea edgeworthii, Canavalia lineata, Gylcine max var. solitae, Glycine max var. yagkong, Glycine max var. hooktae, Glycine max var. bangkong, Glycine max var. geumdu, Glycine max var. chungtae, Glycine max var. mejukong, Glycine soja, Phaseolus radiatus var. geodu, Vicia tetrasperma, Vigna angulasis, and Vigna sinensis inhibited enzyme activity by greatertha 60%. In following study, at the concentration of 0.01 mg/mL, the extracts of C. lineata and V. tetraspermahad relatively strong inhibitory activity against aldose reductase. Because of their potent inhibitory activities, the activity of each solvent fraction from C. lineata and V. tetrasperma was determined, and the potent activity was showed from chloroform and hexane fractions, respectively. {TEX}$IC_{50}${/TEX} values of C. lineata and V. tetrasperma were 0.004 and 0.006 mg/mL, respectively. As a naturally occurring therapeutic agent, leguminous seeds described could be useful for developing new agents of antidiabetic complications.

• Korean Journal of Microbiology
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• v.29 no.2
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• pp.143-147
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• 1991

The root nodules and Glycine max were classified as determinate nodule based on their morphological characteristics, and isolated endosymbiont as a Bradyrhizobium based on its growth rate and single subpolar flagellum. The isolate was similar to B. japonicum USDA110 in utilization of carbon source, growth at 38.deg.C and 2% NaCl, production of $H_{2}$S and especially in the restriction endonuclease digestion pattern of symbiotic genes, allowing them to be placed in sTI group together. The former, however, grew better than the later in broad pH range from 5.0 to 9.5. Infectivity and effectivity of the isolate were confirmed by inoculation of soybean seedlings with the isolates. Characteristics of the reisolated endosymbiont from induced root nodules were identical to those of the first isolate. From these results, it was confirmed that Bradyrhizobium strain isolated from the root nodules of Glycine max was a real symbiont, and was named B. japonicum SNU001.

• Journal of Applied Biological Chemistry
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• v.44 no.1
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• pp.1-5
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• 2001

The methanol extracts of 25 leguminous seeds in vitro was evaluated for inhibitory activities against the small intestinal $\alpha$-glucosidase of Sprague Dawley male rats. The responses varied both with leguminous seed types and concentrations used. At the concentration of 0.5 mg/ml, the methanol extracts of Cassia obtusifolia, Glycine max var. yagkong, Glycine max var. hooktae, Glycine max var. geumdu, Glycine max var. mejukong, Glycine soja, Phaseolus multiflorus, Pisum sativum, and Vigna sinensis inhibited over 50% of the enzyme activity. The extracts of G. max var. yagkong and V. sinensis showed relatively strong inhibitory activities against $\alpha$-glucosidase at the concentration of 0.1 mg/ml. The activity of each solvent fraction from G. max var. yagkong and V. sinensis was determined, and potent activities were detected from chloroform and butanol fractions, respectively. $IC_{50}$ values of G. max var. yagkong and V. sinensis were 0.06 and 0.19 mg/ml, respectively. As a naturally occurring therapeutic agents, leguminous seeds examined could be useful for developing new types of antidiabetic agents.

• The Korean Journal of Ecology
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• v.9 no.1
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• pp.1-7
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• 1986

plastochron index was used as a measure for the nitrate reductase activity (NRA) in Amaranthus retroflexus, Chenopodium album var. centrorubrum and Glycine max. The material plants treated with $NO_3$-N had higher NRA than those treated with ${NH_4}^+$-N. High NRA were found in young tissues which have low PI or LPI in both A. retroflexus and C. album. These results indicated that this enzyme decreased with increasing plastochron index of the extracted tissues. High level of NRA was detected in Glycine max leaves at PI 4.0. On the other hand, there were no significant differences for PI among $NO_3$-treated, ${NH_4}^+$-treated and control in both stems and roots.

• The Korean Journal of Ecology
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• v.15 no.1
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• pp.1-7
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• 1992

The dvelopment of leaves in soybean(glycine max cv. yellow grain and glycine max cv. black grain)plant were assessed for the applicability of the plastochron index. The plastochron ages versus time in days were respectively 6.4 days in black grain and 9.6 days in yellow grain. Plots of plastochron indes(pi) versus time were linear, there were two distinct groups of regression line. when a point of intersection in the lines were upper than the reference length, pi was estimated n+(ln Ln-ln LR)/(ln Ln-ln Ln+2) or (n-1)+(ln Ln-1-ln LR)/(ln Ln-1-ln Ln+1) . When a point of intersection in the lines were less than the reference length, pl was estimated n+(ln Ln-ln LR)/(ln Ln-ln Ln+2) or (n-1)+(ln Ln-1-ln LR)/(ln Ln-1-ln Ln+1) . The growth rate of black grain plants analysed by pl was higher than yellow grain plants.

• Applied Biological Chemistry
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• v.41 no.5
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• pp.399-404
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• 1998

Genipin was obtained from hydrolysis of geniposide isolated from gardenia fruits with ${\beta}-glucosidase$. Reaction of genipin with glycine, alanine, histidine, lysine, phenylalanine and glutamate in aqueous buffer solution converted colorless starting materials to blue pigments. Effect of pH for the formation of blue pigments was tested using UV/Vis spectrophotometer. The optimum pH for the formation of blue pigments was 7.0. No pigment and trace amounts were formed at acidic (pH 3.0) and alkaline (pH 12.0) conditions, respectively. The amount and tincture of blue color were distinct with different amino acids. In contrast with lysine $({\lambda}_{max}=573\;nm)$, glycine $({\lambda}_{max}=595\;nm)$, phenylalanine $({\lambda}_{max}=602\;nm)$ and alanine $({\lambda}_{max}=595\;nm)$, the reaction of genipin with histidine $({\lambda}_{max}=601\;nm)$ and glutamate $({\lambda}_{max}=601\;nm)$ produced relatively small amounts of blue pigments. Rate constants for the formation of blue pigments from genipin with amino acids at various temperatures $(60,\;70,\;80,\;90^{\circ}C,\;pH\;7.0\;phosphate\;buffer)$ were obtained. Rate constants of genipin with basic amino acids were larger than neutral or acidic amino acids. Arrhenius activation energies of the formation of blue pigments indicated that activation energy of glycine $(E_A=9.8\;kcal/mol)$ was especially lower than those of other amino acids $(E_A=13.3{\sim}15.4\;kcal/mol)$.