• 한국식품영양과학회지
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• 제33권5호
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• pp.880-887
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• 2004

된장분말 첨가가 밀가루 반죽과 글루텐 물성에 미치는 효과를 알아보고자 Micro-extensigraph를 이용하여 신장저항도와 신장성을 측정하였고 된장 및 반죽 특성 요소와 빵품질 특성에 대한 상관관계를 조사하였다 시판 된장의 조단백질, 조지방, 염도, 효소 활성 및 유리아미노산 함량은 된장간에 큰 차이를 보였다. 반죽에 된장분말 첨가는 gluten 형성에 필요한 반죽시간의 증가가 요구되며 5.0% 이하의 된장분말첨가로 dry gluten 함량은 유의적으로 증가하였다. 된장분말첨가비율이 증가할수록 밀가루 반죽의 신장저항도는 지속적으로 감소하고 신장성은 일정수준까지는 증가하다가 다시 감소하면서 반죽이 연화되었다. 이러한 현상은 밀가루 반죽에서보다 전분을 제거한 wet gluten에서 뚜렷이 나타났으며, 이는 된장의 protease활성, cystein의 환원작용 등과 관련한다. 특히, protease활성과 cystein 함량이 가장 높은 시판된장 첨가구는 gluten의 신장도와 연화정도가 가장 크게 나타나면서 2.5%첨가수준에서 모든 된장첨가빵 중에서 최대 빵 부피와 가장 부드러운 조직감을 나타내었다. 된장 첨가에 의한 글루텐 함량 증가, 밀가루 반죽 및 wet gluten의 신장성 증가는 최종 방의 부피와 각각 높은 양의 상관성(r=0.76, 0.91, 0.93)을, 빵의 경도와는 음의 상관성을 나타내었다. 그러므로 시판된장 첨가로 인한 빵품질 향상은 gluten 증가와 반죽 신장성 증가에 기인됨을 알 수 있었다.

• 한국식품영양과학회지
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• 제33권6호
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• pp.1043-1048
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• 2004

된장의 대두단백질 분산성이 제빵적성에 어떠한 효과를 나타내는지 알아보고자 전지대두분(FSF; full fat soy flour)과 탈지대두분(DSF; defatted soy flour)을 밀가루 반죽과 빵 배합에 사용하여 gluten물성과 최종 빵 품질을 된장분말 첨가구(2.5%, 5.0%)와 비교하고 측정값들간의 상관관계를 조사하였다. 단백질 분산지수는 된장분말이 57.1%로 FSF 7.3%, DSF 10.8%, 강력분 32.8%보다 유의적으로 높았다. 된장분말 첨가는 wet gluten의 현저한 신장저항도 감소와 신장성 증가를 보였으나 대두분 첨가는 단백질분산지수의 차이로 물성의 변화 정도가 서로 다르게 나타났다. 측정값들 간의 상관관계 조사결과 단백질분산지수는 gluten신장성 (r=0.98, p<0.01)과 오븐팽창력(r=0.88, p<0.05) 증가에 영향을 미치며, 결과적으로 이들은 빵 부피와 큰 상관성을 갖는 것으로 나타났다(p<0.01). 따라서 된장분말의 일정량 사용으로 인한 된장첨가 빵의 부피와 조직감의 현저한 향상은 된장분말의 높은 protease 활성에 의한 단백질분산지수, 반죽의 신장성 증가 및 오븐 팽창력 증가에 의한 것으로 판단된다.

• 한국식품영양과학회지
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• 제28권5호
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• pp.1034-1043
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• 1999

Rheological properties of dough made from waxy barley(Iri28) flour wheat flour mixtures with additives were investigated for the preparation of waxy barley bread using farinograph, extensograph and amy lograph. The water absorption, development time and dough weakness increased as the waxy barley flour level increased in all blends; however, dough stability decreased. Farinogram properties of 10% waxy barley flour added mixture were similar to those of 100% wheat flour. The addition of A.A(ascorbic acid), gluten, HPMC(hydroxy propyl methyl cellulose) improved rheological properties of dough with 30% waxy barley flour added mixture. In particular, stability and weakness of the dough showed greater dough improving effect by addition of A.A. For the extensograph data, strength, resistance and extensibility of dough decreased with increasing level of waxy barley flour. With the addition of additives, extensogram properties were variable for 30% waxy barley flour mixture. Of these additives, gluten had highest value in strength of dough. Addition of A.A and HPMC to 30% waxy barley flour added mixture resulted in an increase in the resistance and a decrease in the extensibility. Waxy barley flour added mixtures showed little higher gelatinization temperature on amylograph data than control. Maximum viscosity reduced as the waxy barley flour level increased. Also 30% waxy barley flour added mixture containing A.A and HPMC showed a decrease in maximum viscosity. But addition of gluten to 30% waxy barley flour mixture resulted an increase in the maximum viscosity. All of 30% waxy barley flour added mixture with additives had lower gelatinization temperature than those without additives. In the SEM images, starch granules were dispersed in a protein matrix. A non continuous, loose protein starch matrix was observed in all waxy barley flour mixture by SEM. Addition of additives gave the dough a more continuous structure with interactions between the starch granule and protein component.

• 한국식품조리과학회지
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• 제24권6호
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• pp.757-762
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• 2008

In this study, korean wheat composite flour and dough were prepared with 1.5, 3.0, 4.5, and 6% Nelumbo nucifera G. tea powder(NNTP). The samples and a control were then compared qualitatively in terms of moisture, protein, ash, and wet gluten content. The farinogram, amylogram, and extensogram characteristics of the dough were also examined, in order to determine the optimal ratio of NNTP for the formulation. According to our results, the moisture content of the flour decreased with increasing NSPP content, whereas its protein and ash content, resistance, and R/E ratio at 135 min of extensogram increased. The NNTP samples had a significantly higher water absorption and weakness of farinogram and maximum resistance at 45, 90, and 135 min. Additionally, samples had an R/E ratio at 45 min of extensogram, 90 min less than the control group. However, stability of the farinogram, temperature of maximum and maximum viscosity of the amylogram, and extensibility of the extensogram showed the reverse effect. The control and NNTP samples showed significant differences in gelatinization beginning temperature of the amylogram, while development time of the farinogram was not significantly different. With regard to the extensogram characteristics of the dough, the area of the control and 1.5% NNTP increased with increasing testing time, whereas at 3.0, 4.5 and 6.0% NNTP, extensibility, and resistance, maximum resistance, and R/E ratio of control and NNTP samples decreased. An area of 3.0, 4.5 and 6.0% NNTP and extensibility of 1.5% NNTP were not significantly different among the testing times. In conclusion, these results show that 1.5% NNTP may prove very useful as a substitute for korean wheat flour where the production of korean wheat white bread is concerned. It may also provide good nutritional and functional properties.

• Food Science and Biotechnology
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• 제16권4호
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• pp.590-593
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• 2007

The textural properties of doughs mixed with L-ascorbic acid (AA), trypsin hydrolyzed gluten peptide (THGP), and a mixture of AA-THGP were investigated using texture analyzer under the fermentation of the full formula and the freezing process. The full formula dough (FFD) required a shorter mixing time than the flour and water formula dough (FWD). The maximum resistance (Rmax) values of both the unfrozen and frozen doughs were lower for the FFD. The effects of AA and THGP additions were not significant (p<0.01) in FFD, however, they were significant in FWD. The freezing effect was significant (p<0.0001) for FFD, indicating that yeast fermented dough was much more sensitive to damage from freezing, which subsequently affected dough strength. Additions of AA (p=0.0026) and THGP (p=0.0097) had a significant effect on the extensibility (E-value) of unfrozen FWD, where THGP increased and AA decreased the E-value. However, freezing did not significantly effect the extensibilities of FWD (p=0.64) or FFD (p=0.21). The area of FFD was lower than the area of FWD for both the unfrozen and frozen doughs. However, the frozen dough mixed with THGP alone had the largest area overall. The addition of additives did not result in significantly different (p<0.01) areas under the curve, except in the frozen FFD. Freezing caused a statistically significant difference in the area of FWD (p=0.0045).

• 한국식품저장유통학회지
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• 제15권4호
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• pp.542-549
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• 2008

당귀 분말의 첨가량에 따른 식빵 반죽의 품질특성에 미치는 영향을 알아보기 위하여 당귀 분말을 첨가한 반죽의 이화학적 특성, 반죽의 물리적 특성을 비교 분석한 결과는 다음과 같다. 당귀 분말의 일반성분 함량은 수분 10.5%, 조단백질 16.9%, 조지방 13.8%, 조회분 6.0%, 식이섬유량 35.5% 등으로 나타났다. 당귀 분말을 첨가한 밀가루의 호화도(RVA)는 당귀 분말의 첨가량이 증가함에 따라 호반죽의 호화도, break down과 set back은 당귀 분말의 첨가량이 증가함에 따라 호화온도와 점도는 감소하였다. Falling number, gluten 함량, 반죽팽창력, mixograph의 결과 모두는 모두 당귀 분말의 첨가량이 증가할수록 감소하였다. Farinogragh 측정에서는 반죽의 점조도, 연화도, 수분 흡수율은 당귀 분말의 첨가량이 증가할수록 증가하였고, 반죽안정도, 반죽 형성시간과 안정도는 감소하였다. Alveograph는 최대압력을 나타내는 P값은 당귀 분말의 첨가량이 증가할수록 낮아졌고, 부피와 신장성도 당귀 분말의 첨가량이 증가할수록 감소하였다. 당귀 분말 첨가량이 증가할수록 반죽의 제빵 적성은 적합하지 않는 것으로 판단되어진다.

• 한국식품과학회지
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• 제35권1호
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• pp.38-43
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• 2003

흑미가루 첨가비율 증가에 따른 반죽의 물리적인 특성을 조사한 결과 흑미가루 첨가량을 증가시켰을 때 회분 함량은 증가하였고 단백질 함량과 gluten 함량은 감소하였다. farino-graph는 흑미가루 첨가량이 증가함에 따라 흡수율, 안정도, 반죽형성시간, 탄력도 및 v/v(valorimeter value)값은 감소하였고 약화도는 증가하여 반죽 시간 및 반죽의 탄력성과 신장성이 감소하였다. Amylograph는 호화개시온도, 최고점도에서의 온도는 낮았으나 최고점도 및 $94^{\circ}C$에서의 점도의 감소로 제빵시 ${\alpha}-amylase$의 활성을 높임으로써 발효능력의 향상을 가져왔다. Extensograph는 신장도, 신장저항도 및 에너지는 감소하였으나 R/E비는 높아짐으로써 제빵성은 흑미가루 첨가량이 증가할수록 낮아졌다. 상기 실험결과 farinograph와 amylograph, extensograph의 측정은 밀가루 중에 함유된 gluten의 신장성 및 신장저항성의 특성, 발효에 따른 발효력 및 가스포집력을 종합한 제빵 특성을 나타내므로 흑미가루가 혼합된 혼합분의 경우 밀가루 100% 시료보다는 단백질 함량의 저하로 제빵 특성치가 낮게 나타나 제빵 특성에 영향을 주므로 단백질원인 활성글루텐이나 산화제 등의 첨가로 반죽의 물성 개선에 관한 실험과 제빵 품질 특성에 대한 실험이 계속 필요하리라 생각된다.

• 한국식품조리과학회지
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• 제30권3호
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• pp.262-270
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• 2014

This study was conducted in order to investigate the properties of bread making and quality improvement when wheat flour is replaced with whole waxy sorghum flour. Sorghum flour, which was prepared with two types of milling methods of pin mill and ultra fine pulverization, was used at different levels ranging from 10, 20 and 30%, respectively. The pasting properties of peak viscosity, setback viscosity and pasting temperature of the composite flour containing pin-milled sorghum flour were higher than those of ultra fine pulverized sorghum flour. The volumes of sorghum bread were lower than that of wheat bread; moreover, they gradually decreased with increasing amounts of sorghum flour, which has inferior dough properties and therefore collapses in the oven. The use of vital gluten (12% based on sorghum flour weight) and emulsifier (SSL; sodium stearoyl lactylate) increased the extensibility and resistance to the extension of the dough, thereby improving its rheological properties. Thus, the oven spring of bread containing sorghum was improved, demonstrating as loaf volume increase up to 15%. However, in the case of breads containing 30% sorghum flour, the loaf volumes were still unacceptably low. Therefore, the formula and the bread making process were further modified as follows: An increase of vital gluten ($12%{\rightarrow}18%$) and shortening ($3%{\rightarrow}6%$), a decrease of mixing time and dough fermentation temperature, and the addition of sorghum flour after gluten development during mixing. The above modifications resulted in the improvement of sorghum bread quality. Therefore, we suggest that pin-milled sorghum flour is more appropriate than ultra fine pulverized sorghum flour for making bread.

• Journal of Plant Biotechnology
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• 제1권1호
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• pp.13-19
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• 1999

The visco-elastic properties of gluten are major determinants of the processing properties of doughs. These visco-elastic properties are strongly influenced by the ratio of monomeric and polymeric proteins and the size distribution of the polymeric proteins, which make up the gluten fraction of the dough. Recent studies have revealed that other features, such as the number of the cysteine residues of the HMW-GS, also play an important role in determining the functional characteristics. To modify the processing properties at molecular level, the relationship between the structure of molecules and dough properties has to be understood. In order to explore the relationships between individual proteins and dough properties, we have developed procedures for incorporating bacterially expressed proteins into doughs, and measuring their functional properties in small-scale equipment. A major problem in investigating the structure/function relationships of individual seed storage proteins is to obtain sufficient amounts of pure polypeptides from the complex families of proteins expressed in the endosperm. Therefore, we have established a simplified model system in which we produce specific protein genes through bacterial expression and test their functional properties in smallscale apparatus after incorporation into base flour. An S poor protein gene has been chosen as a template gene. This template gene has been modified using standard recombinant DNA techniques in order to test the effects of varying the number and position of cysteine residues, and the size of the protein. Doughs have been mixed in small scale apparatus and characterized with respect to their polymeric composition and their functional properties, including dough mixing, extensibility and small scale bating. We conclude that dough characteristics can be manipulated in a predictable manner by altering the cysteine residues and the size of high molecular weight glutenins.

• Current Research on Agriculture and Life Sciences
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• 제5권
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• pp.75-80
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• 1987

냉동발효(醱酵)빵 제조에 사용되는 경질(硬質) 밀가루의 이화학적(理化學的) 성질과 straight no-time method로 조제한 반죽의 동결안정성(凍結安定性)을 검토(檢討)하였다. 밀가루는 수분이 14.0%, 회분(灰分)이 0.48% 정도였고 단백질이 13.15%, 건조 gluten이 13.43%였다. Farinograph성적은 반죽의 development time이 5.5분, 흡수율(吸水率) 62%였고, amylograph성적상의 최고점도(最高粘度)는 500BU정도였다. Extensigraph의 특성중(特性中) 신장저항성(伸張抵抗性)은 시간이 경과할수록 증가하였고, 신장성(伸張性)은 감소(減少)하였다. 이상(以上)의 이화학(理化學)이 성질(性質)로 미루어 공시(供試)밀가루는 제(製)빵에 아주 적합하였다. 동결저장기간과 발효시간이 발효력(醱酵力)에 미치는 영향을 고려할 때, 반죽온도를 $20^{\circ}C$로 조정하는 것이 반죽의 냉동장해(冷凍障害)를 억제하고 냉동안정성(冷凍安定性)을 유지하는데 효과적(效果的)이었다.