• 한국초지조사료학회지
• /
• 제21권1호
• /
• pp.21-26
• /
• 2001

환경 스트레스에 의해 야기되는 활성 산소종에 의한 피해에 내성을 가지는 목초의 개발을 위하여 오차드그래스의 배반 조직 유래의 캘러스에 배추유래의 cytosolic glutathione reductase 유전자(BcGRl)를 Agrobucterium tumefaciens EHA101을 매개로 형질전환시켰다. Hygromcin으로 선발된 캘러스로부터 재분화된 식물체는 야생형과 비교하여 형태적으로 차이를 나타내지 않았다. PCR 및 Southern blot 분석을 통하여 형질전환 식물체의 염색체 내에 BcGRl 유전자가 integration 되었음을 확인하였다. 오차드그래스의 잎으로부터 total RNA를 분리하여 Northern blot 분석을 실시한 결과, 도입된 유전자가 형짙전환 식물체 내에서 지속적으로 발현된다는 것을 확인하였다.

• Archives of Pharmacal Research
• /
• 제28권9호
• /
• pp.1065-1072
• /
• 2005

The nascent thyroglobulin (Tg) multimer molecule, which is generated during the initial fate of Tg in ER, undergoes the rapid reductive depolymerization. In an attempt to determine the depolymerization process, various types of Tg multimers, which were generated from deoxy­cholate-treated/reduced Tg, partially unfolded Tg or partially unfolded/reduced Tg, were subjected to various GSH (reduced glutathione) reducing systems using protein disulfide isomerase (PDI), glutathione reductase (GR), glutaredoxin or thioredoxin reductase. The Tg multimers generated from deoxycholate-treated/reduced Tg were depolymerized readily by the PDI/GSH system, which is consistent with the reductase activity of PDI. The PDI/GSH-induced depolymerization of the Tg multimers, which were generated from either partially unfolded Tg or partially unfolded/reduced Tg, required the simultaneous inclusion of glutathione reductase, which is capable of reducing glutathionylated mixed disulfide (PSSG). This suggests that PSSG was generated during the Tg multimerization stage or its depolymerization stage. In particular, the thioredoxin/thioredoxin reductase system or glutaredoxin system was also effective in depolymerizing the Tg multimers generated from the unfolded Tg. Overall, under the net GSH condition, the depolymerization of Tg multimers might be mediated by PDI, which is assisted by other reductive enzymes, and the mechanism for depolymerizing the Tg multimers differs according to the type of Tg multimer containing different degrees and types of disulfide linkages.

• 한국초지학회:학술대회논문집
• /
• 한국초지조사료학회 2001년도 제25회 정기총회 프로그램 제39회 학술발표회 및 특별강연 초록(한국초지학회)
• /
• pp.83.2-84
• /
• 2001

• The Korean Journal of Physiology
• /
• 제19권2호
• /
• pp.155-160
• /
• 1985

Dantrolene sodium(DS) is a long acting skeletal muscle relaxant which has been successfully used to control muscle spasticity in patients with various neurological disorders. However, its use is associated with hepatotoxicity. Tissue sulfhydryl group has many important roles for cellular integration and glutathione serves as a substrate for the detoxification metabolism. The purpose of this study were to investigate the effect of DS on tissue sulfhydrl group and glutathione content. Foully albino rats were divided into two groups ; saline treated (control) and DS treated groups. DS dissolved in saline was administered orally. All rats were sacrificed after 7. 14. 21 and 28 days of DS ana saline treatment by dacapitation ana liver was removed for the enzyme preparation. Total and nonprotein sulfhydryl were measured by the method of Sedlak and Lindsay (1968). Total glutathione content was assayed according to the method described by Tietze (1969) and glutathione reductase was assayed according to the method of Racker (1955), The results obtained are summarized as follows : DS administration significantly depressed the total, protein and nonprotein sulfhydryl content in liver. There were significant reduction of both total glutathione content and glutathione reductase activity in liver. On the basis of the above results it may be speculated that the toxicity of DS are well correlated with tissue sulfhydryl content and glutathione reductase activity.

• 대한약침학회지
• /
• 제10권1호통권22호
• /
• pp.121-135
• /
• 2007

Objectives : This study was carried out to examine protective effect of wild ginseng extract on HepG2 human hepatoma cell line against tert-Butyl hydroperoxide (t-BHP)-induced oxidative damage. Methods : To evaluate protective effect of wild ginseng extract against t-BHP induced cytotoxicity, LDH level and activity of glutathione peroxidase and reductase were measured. Gene expression was also measured using DNA microarray. Results : Wild ginseng extract showed a significant protective effect against t-BHP-induced cytotoxicity in HepG2 cell line. It is not, however, related with the activities of glutathione peroxidase and glutathione reductase. Analysis of gene expression using DNA chip, demonstrated that 28 genes were up-regulated in t-BHP only group. Five genes - selenoprotein P, glutathione peroxidase 3, sirtuin 2, peroxiredoxin 2, serfiredoxin 1 homolog - may be related with the protective effect of wild ginseng extract. Conclusions : Based on the results, a protective effect of wild ginseng extract against t-BHP-induced oxidative damage in HepG2 cell line is not associated with the activities of glutathione peroxidase and glutathione reductase, but with the expression of selenoprotein P, glutathione peroxidase 3, sirtuin 2, peroxiredoxin 2, and serfiredoxin 1 homolog.

• 농촌의학ㆍ지역보건
• /
• 제19권2호
• /
• pp.107-118
• /
• 1994

Health effects of pesticide among vinylhouse workers in Sangjoo County, Kyungpook Province were assessed by measuring cholinesterase, glutathion reductase, and methemoglobin. Activities of cholinesterases among vinylhouse workers and general farmers were 3.89U and 3.98U in serum and 5.29U and 5.50U in red blood cells, respectively. While levels of methemoglobin between vinylhouse workers(0.16%) and general farmers(0.17%) were very similar, glutathione reductase among vinylhouse workers were 8% lower than that of general farmers. Inhibition of RBC cholinesterase among vinylhouse workers was two times greater than general farmers, suggesting a greater exposure of vinylhouse workers to the organophosphate pesticides. Methemoglobin level among vinylhouse workers with more than or equal to 10 vinylhouse units was 13% higher than that of the workers with less than 10 units. Vinylhouse workers using protective gears during pesticide spray showed higher level of reduced glutathione and lower level of methemoglobin, as compared to the non-users. This indicates that protective gears play an important role against pesticide exposure. Vinylhouse workers practicing good personal hygiene showed a higher level of reduced glutathione, as compared to those with poor personal hygiene, indicating that personal hygiene is also an important factor in reducing pesticide exposure. The present study represents a first attempt to analyze cholinesterase, methemoglobin and glutathione reductase activities among the vinylhouse workers. While neither vinylhouse workers nor general farmers showed abnormal findings among biochemical parameters used in the present study, increased level of methemoglobin and decrease of glutathione reductase activity among vinylhouse workers suggest that these workers have been subject to a higher exposure of pesticide than general farmers.

• The Korean Journal of Physiology and Pharmacology
• /
• 제1권2호
• /
• pp.221-224
• /
• 1997

Effects of melatonin on hepatic glutathione-peroxidase (GSH-Px) and glutathione-reductase (GSH-reductase) activities were studied in Sprague-Dawley (SD) rats administered i.p. (10 mg/kg body weight) with melatonin during 15 days. The activity of cytosolic GSH-reductase in the liver was not changed by melatonin. However, melatonin injection increased significantly the activity of liver cytosolic GSH-Px activity compared with those in saline-treated rats. At the same time, plasma GSH-Px was also increased significantly in melatonin-treated rats. Since GSH-Px, a major antioxidative enzyme, removes $H_2O_2$ and lipid peroxides which are formed during lipid peroxidation from cellular membrane, such elevation of heptatic GSH-Px activity may contribute to the improvement of antioxidative effects under oxidative damage in the liver.

• 대한수의학회지
• /
• 제31권1호
• /
• pp.41-47
• /
• 1991

The protective of influences of sodium selenitc ($Na_2SeO_3$) against the methemoglobinemia with sodium nitrite were investigated on hemoglobin, methemoglobin, glutathione peroxidase and NADH-methemoglobin reductase activity in rabbits which were given 0,1,3 and 9ppm sodium selenite of drinking water for a week. Dietary selenium did not alter total hemoglobin in the blood of rabbits. Selenium was found to decrease nitrite-induced methemoglobin in a dose-dependent manner. The glutathione peroxidase activity was also increased by selenium in all the experimental groups. However, the NADH-methemoglobin reductase activity by selenite did not show significant differences as concerns the methemoglobinemia. These results showed that selenium could inhibit nitrite-induced methemoglobinemia. Its influence of inhibition is suggested that the effect of the reduction of methemoglobin was greatly stimulated by glutathione peroxidase activity.

• 한국식품영양과학회지
• /
• 제22권6호
• /
• pp.702-708
• /
• 1993

Bromobenzene투여시 GE-132의 해독기전을 추구할 목적으로 epoxide생성계와 해독계 효소 및 glutathione 관련 효소계의 활성에 GE-132가 어떤 영향을 주는가를 검토하여 다음과 같은 실험 결과를 얻었다. GE-132(100mg/kg)의 전처리로 cytochrome P-450, amino=pyrine demethylase 및 aniline hydroxylase와 해독계 효소인 epoxide hydrolase활성에는 영향을 미치지 않았으나, glutathions S-transferase활성은 증가 하였다. Bromobenzene(460mg/kg)을 주사하였을때 glutathione S-transferase의 활성이 현저히 감소되던 것이 GE-132의 투여로 대조군 수준으로 증가되었다. 조직내 glutathione의 함량변동도 bromobenzene 투여로 감소되던 것이 GE-132의 전처리로 bromobenzene단독 투여군보다 증가되었으며 ${\gamma}-glutamylcystein$ synthtase의 활성은 각 실험군에서 별다른 영향이 없는데 비해 glutathione reductase의 활성은 bromobenzene투여로 억제 되던 것이 GE-132의 전처리로 대조군 수준으로 활성을 유지하고 있었다.

• 식물조직배양학회지
• /
• 제27권6호
• /
• pp.475-478
• /
• 2000

상추에 particle bombardment를 이용한 배추 Glutathione Reductase (GR)유전자의 형질전환효율 향상 및 형질전환체의 유전분석에 관한 실험을 수행하여 얻은 결과를 요약하면 다음과 같다. Particle bombardment 이용시 사출 전 4시간, 사출 후 16시간 동안 삼투조절제인 0.6M serbitolfmannitol 을 처리하여 형질전환시킨 상추 자엽조직에서 GUS 반응 spot이 가장 많았다. 0.3, 0.6, 1.5 kbp로 나타나는 3가지 종류의 primer를 사용하여 PCR한 결과 각각의 primer에 homology를 타나내는 band를 확인하여 355 promoter와 BcGRl유전자가 삽입이 된 것을 확인하였다. 형질전환된 상추의 자식 종자를 채종하여 kanamycin 200mg/L이 첨가된 MS배지에 파종하여 형질전환시키지 않은 상추의 자식 종자와 비교해 본 결과, 형질전환된 상추의 종자에서 발아된 유묘의 약 70%정도가 kanamycin에 대해 내성을 나타내었다.