• Toxicological Research
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• 제34권2호
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• pp.151-162
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• 2018

Anti-diabetes activity of Catharsius molossus (Ca, a type of dung beetle) glycosaminoglycan (G) was evaluated to reduce glucose, creatinine kinase, triglyceride and free fatty acid levels in db mice. Diabetic mice in six groups were administrated intraperitoneally: Db heterozygous (Normal), Db homozygous (CON), Heuchys sanguinea glycosaminoglycan (HEG, 5 mg/kg), dung beetle glycosaminoglycan (CaG, 5 mg/kg), bumblebee (Bombus ignitus) queen glycosaminoglycan (IQG, 5 mg/kg) and metformin (10 mg/kg), for 1 month. Biochemical analyses in the serum were evaluated to determine their anti-diabetic and anti-inflammatory actions in db mice after 1 month treatment with HEG, CaG or IQG treatments. Blood glucose level was decreased by treatment with CaG. CaG produced significant anti-diabetic actions by inhiting creatinine kinase and alkaline phosphatase levels. As diabetic parameters, serum glucose level, total cholesterol and triglyceride were significantly decreased in CaG5-treated group compared to the controls. Dung beetle glycosaminoglycan, compared to the control, could be a potential therapeutic agent with anti-diabetic activity in diabetic mice. CaG5-treated group, compared to the control, showed the up-regulation of 48 genes including mitochondrial yen coded tRNA lysine (mt-TK), cytochrome P450, family 8/2, subfamily b, polypeptide 1 (Cyp8b1), and down-regulation of 79 genes including S100 calcium binding protein A9 (S100a9) and immunoglobulin kappa chain complex (Igk), and 3-hydroxy-3-methylglutaryl-CoenzymeAsynthase1 (Hmgcs1). Moreover, mitochondrial thymidine kinase (mt-TK), was up-regulated, and calgranulin A (S100a9) were down-regulated by CaG5 treatment, indicating a potential therapeutic use for anti-diabetic agent.

• Journal of Ginseng Research
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• 제37권2호
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• pp.187-193
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• 2013

The effect of Korean red ginseng (KRG) on diabetic renal damage was investigated using streptozotocin (STZ)-induced diabetic rats. The diabetic rats showed loss of body weight gain, and increases in kidney weight and urine volume, whereas the oral administration of KRG at a dose of 100 or 250 mg/kg of body weight per day for 28 d prevented these diabetes-induced physiological abnormalities. Among the kidney function parameters, elevated plasma levels of urea nitrogen and creatinine in diabetic control rats tended to be lowered in KRG-treated rats. In addition, administration of KRG at a dose of 100 mg/kg body weight in the diabetic rats showed significant decreases in serum glucose and tumor necrosis factor-${\alpha}$ (TNF-${\alpha}$), implying that KRG might prevent the pathogenesis of diabetic complications caused by impaired glucose metabolism and oxidative stress. KRG also significantly reduced advanced glycation end product (AGE) formation and secretion from kidney of diabetic rats. Furthermore, KRG decreased the levels of N-(carboxymethyl) lysine and expression of AGE receptor. KRG also reduced the overexpression of cyclooxygenase-2 and inducible nitric oxide synthase in the kidney via deactivation of nuclear factor-kappa B. We also found that KRG prevented STZ-induced destruction of glomerular structure and significantly suppressed high glucose-induced fibronectin production. Taken together, KRG ameliorates abnormalities associated with diabetic nephropathy through suppression of inflammatory pathways activated by TNF-${\alpha}$ and AGEs. These findings indicate that KRG has a beneficial effect on pathological conditions associated with diabetic nephropathy.

• Journal of Ginseng Research
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• 제14권2호
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• pp.117-121
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• 1990

Browning intensity is a major factor to estimate the quality of red ginseng or red ginseng products. The Maillard type of browning reaction proceeds nonenzymatically during extraction and concentration of red ginseng. The present studies were carried out to investigate the effects of amino acids and sugars on the browning reaction during extraction and concentration of red ginseng. Red ginseng was pulverized to 115 mesh and then tenfold (v/w) of water was added to the powder to make the substrate of red ginseng. Solution (0.1 M) of fourteen amino acids and of folly silgars were added to the substrates of red ginseng powder and these were then extracted and concentrated to examine their browning intensities. Amino acids were more effective than sligars in acrelerating the browning reaction. Acceleration of the browning reaction in the concentrate was in the order of arginine> histidine>glycine>alanine>lysine phenyl alanine>aspartic acid>lelicine>threonine>gllitamic acid>tyrosine>valine>istleucine>methionine for amino acids, and was glucose>frlictose >silcrose, maltose for sugars.

• Journal of Microbiology and Biotechnology
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• 제10권2호
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• pp.187-194
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• 2000

An actinomycetes strain, T-2, which produces transglutaminase (EC 2.3.2.13), was isolated from soil and identified as belonging to the Actinomadura sp., based on taxonomc studies. The conditions for the transglutaminase production and its enzymatic properties were investigated. The optimum components for the transglutaminase production were 2% glucose, 1% polypeptone and soytone, and 0.1% MnCl2. The optimum pH and temperature of the enzyme reaction were pH 8.0 and $45^{\circ}C$, respectively. The enzyme was stable within the pH range of 5.0-9.0 and $30^{\circ}C-45^{\circ}C$. The novel enzyme required no calcium ions for its activity. This enzyme polymerized various proteins such as casien, soy protein, hemoglobin, egg white, gelatin, and soybean milk.

• Food Science and Biotechnology
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• 제16권1호
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• pp.110-115
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• 2007

In this study we investigated the volatile compounds that are generated in sesame and contribute to its characteristic flavor. Different reaction systems were used to examine how certain amino acids influenced flavor profiles, and also to evaluate the effects of sugar types on the distribution of those volatile compounds. The volatiles that were generated in each reaction system were selectively isolated and analyzed by gas chromatography and gas chromatography-mass spectrometry, respectively. Among the 20 identified compounds, nitrogen-containing alkylpyrazines were found to be the predominant volatiles. The alkylpyrazine amounts varied across the different model systems, with the total yield being highest in the arginine reaction mixture, followed by the alanine, serine, and lysine mixtures. In general, fructose generated the most extensive amount of volatiles compared to glucose and sucrose. However, the yield of specific flavor compounds varied according to the type of sugar used. Finally, the results clearly showed that a reaction temperature of $135^{\circ}C$ and a reaction time of 20 min generated the highest amount of volatile compounds.

• Asian-Australasian Journal of Animal Sciences
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• 제27권1호
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• pp.83-92
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• 2014

This experiment was conducted to evaluate the effects of dietary CP levels in gestation under equal lysine content on reproductive performance, blood metabolites and milk composition of gilts. A total of 25 gilts ($F_1$, Yorkshire${\times}$Landrace) were allotted to 4 dietary treatments at breeding in a completely randomized design, and fed 1 of 4 experimental diets containing different CP levels (11%, 13%, 15%, or 17%) at 2.0 kg/d throughout the gestation. Body weight of gilts at 24 h postpartum tended to increase linearly (p = 0.09) as dietary CP level increased. In lactation, backfat thickness, ADFI, litter size and weaning to estrus interval (WEI) did not differ among dietary treatments. There were linear increases in litter and piglet weight at 21 d of lactation (p<0.05) and weight gain of litter (p<0.01) and piglet (p<0.05) throughout the lactation as dietary CP level increased. Plasma urea nitrogen levels of gilts in gestation and at 24 h postpartum were linearly elevated as dietary CP level increased (p<0.05). Free fatty acid (FFA) levels in plasma of gestating gilts increased as dietary CP level increased up to 15%, and then decreased with quadratic effects (15 d, p<0.01; 90 d, p<0.05), and a quadratic trend (70 d, p = 0.06). There were no differences in plasma FFA, glucose levels and milk composition in lactation. These results indicate that increasing dietary CP level under equal lysine content in gestation increases BW of gilts and litter performance but does not affect litter size and milk composition. Feeding over 13% CP diet for gestating gilts could be recommended to improve litter growth.

• 한국수산과학회지
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• 제15권4호
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• pp.303-311
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• 1982

잉어 및 가물치의 정미성분을 밝힐 목적으로 전보(양과 이 : 1979, 1980-a, 1980-b, 1982)에서 보고한 천연산 잉어 및 가물치의 분석치를 기초로 하여 표준시약을 가지고 합성 엑스분을 조제하여 천연, 엑스분과 맛을 비교하였으며, omission test를 실시하여 각 성분들이 맛에 미치는 영향을 조사하였다. 관능검사 결과, 잉어는 glycine, glutamic acid, IMP, 숙신산 및 $Na^{+}$이 맛의 핵심을 이루고 있었고 arginine, lysine, threonine, alanine, inosine, hypoxanthine, 포도당, $Cl^{-}$ 및 $PO_{4}^{3-}$에 의해서 더욱 맛이 조화를 이루고 있었다. 가물치는 glycine, alanine, glutamic acid, IMP, $Na^{+}$ 및 $PO_{4}^{3-}$등 6종 성분이 주된 정미성분이었다.

• 한국식품영양학회지
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• 제12권5호
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• pp.470-477
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• 1999

The purpose of this study was to choose prinicipal food components contained in diet foods and food additives used for manufacturing processed foods and elucidate their in vivo effects on the growth pattern of Helicobacter pylori. To do this the antibacterial effects of various sources of carbon nitrogen and mineral as an effect agent on Helicobacter pylori were first assessed based upon bacterial growth degree. results show that the source of carbon tested had different effects on bacterial growth of Helicobacter pylori. It was revealed that a promotional effect of monosaccharides resulted in enhanced growth of Helicobacter pylori compared with disaccharides and polysacchrides, in particular glucose was observed to be most effective in growth of Helicobacter pylori among monosaccharides teste whereas mannose to hinder the growth of Helicobacter pylori. Polyols such as sorbitol mannitol maltitol and xylitol was however observed to show no promotion or suppression effect on growth of Helicobacter pylori. Apart from these the sources of amino acid and inorganic nitrogen were chosen and tested to assess the promotion or suppression effect of nitrogen sources on growth of helicobacter pylori. It was found that amino acid such as lysine showed its promotion effect on the growth of Helicobacter pylori while arginine (NH4)2SO4 and NH4Cl showed no effect on its growth. Ammoia and urea were however observed to have a positive effect on the growth of Helicobacter pylori. Among these effect agents lysine and methionine were revealed to show the most positive effect on growth of Helicobacter pylori. Minerals such as MgSO4 KH2PO4 and MgCl2 appered to exert their positive growth effects whereas CaCl2 and CaSo4 had a little effect. In addition FeSO4 FeCl2 and FeCl3 brought suppression on the growth of helicobacter pylori. In studies of the growth of Helicobacter pylori by food additives ascorbic acid showed extreme suppression on its growth,. Sodium nitrate and sodium chloride were also found to be of negative effect on the growth of Helicobacter pylori in rder of degree whereas tocopherol had nothing to do with microbial growth.

• Journal of Applied Biological Chemistry
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• 제64권2호
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• pp.141-151
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• 2021

Insects affect crop harvest yield and quality, making plant response mechanisms to insect herbivores a heavily studied topic. However, analysis of plant responses to honeybees is rare. In this study, comprehensive metabolic profiling of Arabidopsis thaliana exposed to honeybees was performed to investigate which metabolites were changed by the insect. A total of 85 metabolites-including chlorophylls, carotenoids, glucosinolates, policosanols, tocopherols, phytosterols, β-amyrin, amino acids, organic acids, sugars, and starch-were identified using high performance liquid chromatography, gas chromatography-mass spectrometry, and gas chromatography-time-of-flight mass spectrometry. The metabolite profiling analysis of Arabidopsis exposed to honeybees showed higher levels of stress-related metabolites. The levels of glucosinolates (glucoraphanin, 4-methoxyglucobrassicin), policosanols (eicosanol, docosanol, tricosanol, tetracosanol), tocopherols (β-tocopherol, γ-tocopherol), putrescine, lysine, and sugars (arabinose, fructose, glucose, mannitol, mannose, raffinose) in Arabidopsis exposed to honeybees were higher than those in unexposed Arabidopsis. Glucosinolates act as defensive compounds against herbivores; policosanols are components of plant waxes; tocopherols act as an antioxidant; and putrescine, lysine, and sugars contribute to stress regulation. Our results suggest that Arabidopsis perceives honeybees as a stress and changes its metabolites to overcome the stress. This is the first step to determining how Arabidopsis reacts to exposure to honeybees.

• 한국식품영양과학회지
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• 제10권1호
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• pp.1-16
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• 1981

해삼내장(內臟)젓은 그 향미(香味)가 독특(獨特)하여 기호식품(嗜好食品)으로서 널리 애용(愛用)되고 있으나 그의 맛성분(成分)에 대한 연구보고(硏究報告)는 없다. 그래서 내장(內臟)젓의 맛성분(成分)을 밝혀 식품영양학적(食品營養學的) 기초자료(基礎資料)를 얻고져 삼천포산(三千浦産) 해삼 내장(內臟)을 원료(原料)로 써 숙성(熟成) 중(中) 유리(遊離)아미노산(酸), 유리당(遊離糖), 핵산관련물질(核酸關聯物質), TMAO, TMA 및 betaine의 변화(變化)를 실험(實驗)하였다. 해삼내장(內臟)의 유리(遊離)아미노산(酸) 조성(組成)을 보면 함량(含量)이 많은 것은 glutamic acid, alanine, glycine 및 proline 이고 함량(含量)이 적은 것은 leucine, valine phenylalanine, isoleucine, methionine 및 tyrosine 등이었다. 함량(含量)이 많은 아미노산(酸)의 전유리(全遊離)아미노산(酸)에 대한 비율을 보면 glutamic acid가 32.3%, alanine 이 16.4%, glycine이 12.0%, proline 이 10.54%로서 이들 4종(種)의 아미노산(酸)이 전유리(全遊離)아미노산(酸)의 71.2%를 차지하였다. 젓갈 숙성중(熟成中) 유리(遊離)아미노산(酸) 조성(組成)에는 변화(變化)가 없으나 원료(原料)에 많았던 glutamic acid, alanine, glycine proline, lysine, arginine 및 leucine 등은 숙성기간(熟成期間)에 따라 양적(量的) 변화(變化)는 있었지만 대체(大體)로 보아 젓갈 제품(製品)에도 함량(含量)이 많았다. 해삼내장(內臟)젓의 유리당(遊離糖)으로서는 galactose 가 $933.7{\sim}938.0mg%$로서 월등히 많았고, arabinose가 78.7mg%, xylose가 $55.2{\sim}77.1mg%$였으며 도당(萄糖)은 흔적량이었다. 핵산관련물질(核酸關聯物質)은 젓갈 숙성(熟成) 중(中) 대체(大體)로 증가(增加)하는 경향(傾向)이었고 특(特)히 함량(含量)이 많은 것은 hypoxanthine으로서 $47.1{\sim}62.5{\mu}mole/g$였으며, 정미성(呈味性)이 강(强)한 lMP도 비교적(比較的) 함량(含量)이 많았다. 젓갈 숙성(熟成) 중(中) TMA는 증가(增加)하는 반면(反面) TMAO는 점차 감소(減少)하는 경향(傾向)이었으며, 해삼내장(內臟)젓에는 TMAO질소(窒素)가 30.0mg% 전후(前後)로서 비교적(比較的) 함량(含量)이 많았다. 젓갈 숙성(熟成) 중(中) betaine은 점차 증가(增加)하는 경향(傾向)이었고, 해삼내장(內臟)에 545.0mg%, 젓갈에는 $734.2{\sim}934.2mg%$로서 월등히 그 함량(含量)이 많았다. 해삼내장(內臟)젓의 정미성분(呈味成分)으로서는 좋은맛을 가진 glutamic acid, 단맛을 가진 alanine, glycine, lysine, proline, arginine, 쓴맛을 가진 leucine을 주체(主體)로 한 유리(遊離)아미노산(酸), 그리고 galactose, arabinose 및 xylose등의 유리당(遊離糖), 단맛을 가진 betaine TMAO 및 핵산관련물질(核酸關聯物質)로서는 lMP, hypoxanthine 등이 중요(重要)한 성분(成分)이고 이들 성분(成分)들이 해삼내장(內臟)의 독특(獨特)한 향기(香氣)와 texture등과 조합(組合)되어 해삼내장(內臟)젓의 풍미(風味)에 중요(重要)한 구실을 할 것이라는 결론(結論)을 얻었다.