• Title/Summary/Keyword: gluconate-negative mutant

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Effect of Gluconic Acid on the Production of Cellulose in Acetobacter xylinum BRC5

  • PARK, SANG TAE;TAEKSUN SONG;YOUNG MIN KIM
    • Journal of Microbiology and Biotechnology
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    • v.9 no.5
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    • pp.683-686
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    • 1999
  • Four mutants of Acetobacter xylinum BRC5 defective in gluconic acid production were isolated from UV-irradiated cells. The gluconic acid-negative mutants did not show glucose oxidase activity. The mutants were also defective in cellulose production. A randomly selected mutant grown in the Hestrin-Schramm medium (pH 6.0) supplemented with gluconic acid, however, was found to synthesize cellulose. The mutant grown in Hestrin-Schramm medium whose pH was adjusted to 5.0 with HC1 and contained no gluconic acid also produced cellulose. Wild-type cells grown under the same condition synthesized cellulose more rapidly than those grown in the pH 6.0 medium.

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