• Korean Journal of Plant Resources
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• v.16 no.1
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• pp.40-48
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• 2003

Cylindrocarpon destructans is the major pathogen inducing the root rot disease in ginseng. Up to now, there is no reliable and convenient method to analyze the spore density or population of this pathogen in ginseng-growing soil or any contaminated farmlands. Therefore, it will be very valuable to develop a new and reliable method in detecting the spore of this pathogen. In this study, a molecular biological technique using two step nested PCR method, was developed. Two universal ITS primers, ITS5F and ITS4R were used in the first round of PCR to amplify a fragment of ITS region from the genomic DNA of C. destructans. The specific prmers Nest 1 and Nest 2 were designed and used in the second round of PCR to amplify a inner fragment from the first round PCR product of C. destructans. C. destructans spore, only soil samples from the diseased ginseng farm produced the positive bands, suggesting its usefulness in detecting the C. destructans spores in soil samples. Thus it is recommended to first extract the whole genomic DNA from soil samples and use it for the PCR reaction, thereby eliminating the inhibitory activity of soil components.

• Korean Journal of Environmental Agriculture
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• v.21 no.4
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• pp.286-290
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• 2002

This work has been conducted to investigate the behavior of pesticides in ginseng(Panax ginseng C. A. Meyer) cultivation environment, with a fungicide procymidone as a model pesticide. Procymidone adsorption on ginseng cultivating soil was studied and persistency of procymidone in soil was monitored in indoor incubation experiments at 25$^{\circ}C$ and 10$^{\circ}C$. The soil adsorption coefficients($K_{oc}$) of procymidone were in the range of 513$\sim$743 suggesting the mobility of procymidone in soil is relatively low. Procymidone showed higher persistency in soil under indoor incubation condition than outdoor field condition. The half lives estimated from the first order reaction kinetics were 248 days and 330 days at 25$^{\circ}C$ and 10$^{\circ}C$, respectively.

• Journal of Ginseng Research
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• v.14 no.1
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• pp.63-66
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• 1990

The development of cracking in ginseng roots was studied to elucidate the factors affecting it in the field. The cracking of 6-year-old ginseng roots harvested in late October callld be induced in 2 days after soaking in water or in watersatllrated soil. However, it callld be slowed down by approximately 10 days when the roots were kept at a low temperature(5$^{\circ}C$). Roots excavated in late May did not develop cracking tinder the conditions tested. There was no significant difference between rice-straw shade and P.E. net shade in the rate of cracked ginseng roots. Which varied with ginseng varieties in relation to shade material. The rate of cracked roots was about 40% in 6-year plants, but it was extremely high (55.6%) in rusty roots. Healing of the wound formed by cracking was generally low but varied between harvest years.

• 한국약용작물학회:학술대회논문집
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• 2008.05a
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• pp.79-80
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• 2008

• Proceedings of the Ginseng society Conference
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• 1984.09a
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• pp.57-62
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• 1984

Four year old American ginseng plants (Panax quinquefolium L.) were grown in control and treated field plots in North Carolina, USA. Soil pH (4.4, 5.5, and 6.5), soil phosphate (19, 89 and 232 ppm) and mulch treatments (wheat straw, pine needle straw, poplar bark, oak bark, pine bark and hardwood leaves) were studied for their effects on total dry weight, total ginsenosides and 5 individual ginsenosides (A1, Rg1, Rd, Re, and Rb2). The leaf and root tissue were analyzed for ginsenosides by high pressure liquid chromatography (HPLC). The oak and poplar bark mulch treatments appeared to have the best effect upon the growth and production of roots while not significantly decreasing the ginsenoside content of the roots. The oak mulch showed a statistical increase in the ginsenoside content of the leaves.

• Research in Plant Disease
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• v.7 no.1
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• pp.25-30
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• 2001

Influence of soil flooding with organic matters amendment at various temperatures on population changes of fungi, including Fusarium, and bacteria in ginseng-replanting field soils was examined to evaluate the effective flooding conditions for reducing the progress of ginseng root rot. Populations of Fusarium spp. and total fungi in flooded soils declined with days after flooding. The higher was the temperature in range of 20$\^{C}$ to 35$\^{C}$, the greater was the effect of flooding on the decrease of the fungal population. Flooding of soils with organic matters amendment had synergistic effect on the decrease of the fungal population at the same temperature; Fungal populations in flooded soils with and without organic matters amendment were reduced to 1/100 and 1/10, respectively, relative to those in non-flooded soils after 60 days of treatment at 30$\^{C}$. rice straw seemed to be more effective than greens. Population changes of total bacteria in flooded soils were similar to the trend of total fungi. However, the flooding seemed to influence less effect on the bacterial population than on the fungal population. Based on these experiments, we suggest than the progress of ginseng root rot in ginseng-replanting field soils may be significantly reduced by flooding them for longer than 3 months near at 30$\^{C}$ after amendment of organic matters.

• Journal of the Korean Society of Tobacco Science
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• v.16 no.2
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• pp.128-133
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• 1994

This study was carried out to get agronomic information about the tobacco culture in paddy soil where incidence of grey tobacco leaves used to appearly uptake of iron, manganese and chlorine due to soil acidity and high water level. Application of lime to paddy soil(pH5.4-5.6) reduced content of iron, manganese known as proceeding materials giving rise to variegated grey tobacco after curing, compared with non-treatment. Grey leaves were found mainly at lower and middle stalk positions, and incidence of grey tobacco was lowered by application of lime in the well drained field but was not affected by level of fertilizer application. Amendment of soil acidity by lime tended to decrease chlorine and manganese content in leaves. Nicotine and mangenese content of leaves were lowered by reduction of one-quarter fertilizer level. In case of lime treatment, increase of yield reached to 4-6% comparing with those of non-treatment but price per kg was not affected. Reduction of N fertilizer level to three-quarters had the equal yield but high quality of leaf comparing with standard fertilization in paddy field.

• Journal of Plant Biotechnology
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• v.30 no.2
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• pp.167-172
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• 2003

Immature zygotic embryos of Eleutherococcus senticosus seeds matured rapidly within one month when the seeds comprising zygotic embryos were pieced to small size and cultured on 1/2 MS medium. Frequency of somatic embryos formation was declined rapidly when the zygotic embryos germinated and grew to plantlets. Embryogenic cells were induced by consecutive subculture of somatic embryos on MS medium with 1.0mg/L2,4-D. After heart-shaped somatic embryos were induced by suspension culture, these embryos were plated onto petri dish to support maturation of embryos. Germination of embryos occurred on medium with 5mg/L GA$_3$and transferred to culture bowl to stimulate the further growth. Frequency of soil survival of plantlets was influenced by soil mixture (perlite and peatmoss). The suitable combination of perlite and peatmoss was 1:5, and the soil survival rate was 78% after 4 months. The soil transferred plantlets were over-wintered in field condition after defoliation. New year sprouting of plants was achieved successfully and they grew to adult plants. These results indicate that the systematic procecure of plant production in E. senticosus for micro propagation.

• Korean Journal of Medicinal Crop Science
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• v.15 no.3
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• pp.220-226
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• 2007

For the analysis of hazard factors and the development of GAP (Good Agricultural Practices) Ginseng, 10 ginseng cultural farms wished certified GAP were selected at Geumsan-gun area, a representative site of ginseng cultivation in Korea. In order to verify the safety of GAP ginseng, possible contamination of pesticide and heavy metal residues, and microbial hazard were analyzed. Soil and water around ginseng cultivation field, and ginseng were investigated. Eighty-one pesticides including carbendazim were used as typical pesticide against plant pathogens and insect pests of ginseng plant and general crops. There was no excess the maximum residue limit (MRL) in residue figure of the soil. Including the residue figure of the arsenic (0.81 ml/kg) and 7 other heavy metals was also suitable to cultivate the ginseng plant. The irrigation water and dilution water for pesticide application were also safety level for GAP. Fresh ginsengs from the farms were sampled and investigated pesticide residues and contaminations of bacteria. Among 23 pesticides tested, we didn't detect any kinds of pesticide residues, but tolclofos-methyl was frequently found in the other ginseng field. On the investigation of microorganism hazards, 2 gram negative bacteria and 1 gram positive bacterium were found in the fresh ginseng. Number of total bacteria was $1.5{\times}10^3$ cfu/ml, which was less than the other agriculture products. At these results, 10 selected ginseng farms were good cultural places for GAP ginseng production and the ginseng cultured from Geumsan-gun area were a good safe far human.

• Journal of Ginseng Culture
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• v.4
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• pp.128-141
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• 2022

Baengnyeongdo Island, which belongs to Ongjin-gun, Incheon, is an island in the northernmost part of the West Sea in South Korea. Baengnyeong Island is the 15th largest island in Korea and covers an area of 51 km². The Korea Ginseng Corporation (KGC) investigated the possibility of growing ginseng on Baengnyeong Island in 1996. In 1997, thanks to the support of cultivation costs from Ongjin-gun, the first ginseng seedbed was built on Baengnyeong Island. In 1999, the seedlings were transplanted to a permanent field under a contract with KGC. In 2003, the first six-year-old ginseng harvest was performed, and KGC purchased all production according to the contract. Since then, KGC has signed on to grow ginseng until 2012 and purchased six-year-old ginseng until the fall of 2016. Since 2014, the GimpoPaju Ginseng Agricultural Cooperative Association has signed a ginseng production contract. According to a survey of nine 6-year-old ginseng fields (total 5,961 units) on Baengnyeong Island, the top five with good growth had a survival rate of 42.6 to 68%, and the bottom four with poor growth had an extremely low survival rate of 11.1 to 21.3%. The four fields with low survival rates were where hot peppers were planted before ginseng cultivation. It is believed that the excess nitrogen remaining in the soil due to the treatment of compost or manure during pepper cultivation causes ginseng roots to rot. The average incidence of Alternaria blight was 8.6%. Six six-year-old ginseng gardens were low at 1.1 to 4.7%, while the other three were high at 16.7 to 20.9%. It is assumed that the reason for the low survival rate and high incidence of Alternaria blight is a rain-leaking shield. Farmers used rain-leaking shields because the precipitation on Baengnyeong Island was smaller than on land. One field showed 3% of leaves with yellowish brown spots, a symptom of physiological disturbance of the leaf, which is presumed to be due to the excessive presence of iron in the soil. To increase the production of ginseng on Baengnyeong Island, it is necessary to develop a suitable ginseng cultivation method for the island, such as strengthening the field management based on the results of a scientific study of soil, using rain-resistant shading, and installing drip irrigation facilities. I hope that ginseng will become a new driving force for the development of Baengnyeong Island, allowing ginseng products and food to thrive in the beautiful natural environment of the island.