• Journal of Ginseng Research
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• 제15권3호
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• pp.197-199
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• 1991

Growth of ginseng and physical properties of soil were compared with bed height in experimental plots of sand loam for four year and clay loam soil for three year old ginseng plants, respectively. Field survey was also carried out to compare yield and soil physical properties with bed height in the same fold of six years old ginseng fields. High yield of ginseng root was observed at high bed both in the experimental plots and field survey as well. The rate of rusty root was significantly reduced at high bed. Soil porosity increased but soil hardness decreased at high bed.

• 한국식생활문화학회지
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• 제21권5호
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• pp.559-564
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• 2006

This study was done for the determination of ginsenosides contents of Korean ginseng and ginseng products as well as the development of analytical method for ginsenosides. It is known that perfect segregation of ginsenoside Rg and Re is not easy, but in this study almost perfect segregation can be possible by the control of concentration between acetonitrile and water. Among Korean ginseng, ginseng powdered tea and red ginseng powdered tea, the highest ginsenosides content of sum of each 7 kind o ginsenoside was found in red ginseng powdered tae as 23,211${\mu}g$ per 1g/dw The ginsenoside content of ginseng powdered tea was lower than red ginseng powdered tea as 15,217${\mu}g$ per 1g/dw Total ginsenoside content in the root of ginseng was 29,268${\mu}g$ per 1/dw Each amount of ginsenoside contained in ginseng root was in the order of Rb1, Rg1, and Rc. It was shown that there was difference in constitutional element of ginsenosides in ginseng powdered tea and ginseng root.

• Journal of Ginseng Research
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• 제5권1호
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• pp.35-40
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• 1981

Explants of mature root tissues and calli derived from root and cotyledon of Panax ginseng were cultured in vitro on Murashige and Skoog medium supplemented with 2, 4-dichlorophen-oxyacetic acid(3,4-D), naphthaleneacetic acid(NAA), benzyladenine, and gibberellic acid to assess their capacity to regenerate organs. Root formation at high percentage (46.2-61.1%) was obtained 20-30 days after culturing on media supplemented with combinations of NAA(5 mg/l) and kinetin (1 mg/l), And calli derived from cotyledon produced numerous embryoids in media($\frac{1}{2}$MS) containing 2,4-D(0.5 mg/l) and kinetin (0.5 mg/l). Reculture of these embryoids in media($\frac{1}{2}$MS) enriched with 1 mg/l of benzyladenine and 1 mg/l of gibberellic acid resulted in more plantlet regeneration.

• Journal of Ginseng Research
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• 제35권2호
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• pp.209-218
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• 2011

Ginseng has been used as a general tonic agent to invigorate the human body as an adaptogenic agent. In a previous report, we have shown that ginseng contains a novel glycolipoprotein called gintonin. The main function of gintonin is to transiently enhance intracellular free $Ca^{2+}$ $[Ca^{2+}]_i$ levels in animal cells. The previous method for gintonin isolation included multiple steps using organic solvents. In the present report, we developed a simple method for the preparation of crude gintonin from ginseng root as well as stem and leaf, which produced a higher yield of gintonin than the previous one. The yield of gintonin was 0.20%, 0.29%, and 0.81% from ginseng root, stem, and leaf, respectively. The apparent molecular weight of gintonin isolated from stem and leaf through sodium dodecyl sulfate polyacrylamide gel electrophoresis was almost same as that from root but the compositions of amino acids, carbohydrates or lipids differed slightly between them. We also examined the effects of crude gintonin from ginseng root, stem, and leaf on endogenous $Ca^{2+}$-activated $Cl^-$ channel (CaCC) activity of Xenopus oocytes through mobilization of $[Ca^{2+}]_i$. We found that the order of potency for the activation of CaCC was ginseng root > stem > leaf. The $ED_{50}$ was $1.4{\pm}1.4$, $4.5{\pm}5.9$, and $3.9{\pm}1.1$ mg/mL for root, stem and leaf, respectively. In the present study, we demonstrated for the first time that in addition to ginseng root, ginseng stem and leaf also contain gintonin. Gintonin can be prepared from a simple method with higher yield of gintonin from ginseng root, stem, and leaf. Finally, these results demonstrate the possibility that ginseng stem and leaf could also be utilized for ginstonin preparation after a simple procedure, rather than being discarded.

• 한국식생활문화학회지
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• 제16권5호
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• pp.478-482
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• 2001

This study was conducted to investigate the difference of general feature and ginsenoside content of 6 years old ginseng root among different grade of roots. Total weight of a 1st grade-6 years old ginseng root was 115.1g and weight, length, diameter and specific gravity of main root were 64.68g, 8.39cm, 3.31cm and 0.96, respectively. Main root of 1st grade ginseng root was larger in size and specific gravity and more heavy than that of End or 3rd grade of the roots. Though crude saponin contents were not so different among the different grade of roots, but ginsenoside Rb1, Rg1 and Re content were higher in 1st grade of root than that of 2nd or 3rd grade of root. Those ginsenosides were located mainly in periderm and cortex.

• 한국약용작물학회지
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• 제19권3호
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• pp.157-161
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• 2011

Growth characteristics, root yield and ginsenoside contents of 3-year-old ginseng in greenhouse shaded by $30^{\circ}$ sloped-curtain made of aluminum were compared to traditional shade facility in order to develop cultural practice for organic ginseng. Light transmittance ratio in greenhouse with $30^{\circ}$ sloped-curtain shade was distinctly lower than that of traditional shade from sunrise to 9 a.m., while its ratio in greenhouse was higher than traditional shade since 9 a.m. due to the reflection of light. Air temperature of greenhouse was $1.3^{\circ}C$ higher than that of traditional shade on the first ten days of August due to more reflected light. Root yield of greenhouse was 44% higher than that of traditional cultivation because of the inflow of reflected light and the decrease of disease of Alternaria and Anthracnose by blocking rainfall. Dry matter partitioning ratio of rhizome and lateral root were increased in ginseng cultivated at greenhouse due to longer survival time in leaf than traditional cultivation. Total ginsenoside contents cultivated at greenhouse was decreased in the part of taproot, while it was increased in the part of lateral and fine root compare to traditional cultivation. Individual ginsenoside contents between greenhouse and traditional cultivation showed significant difference more frequent in fine root than taproot and lateral root. Total ginsenoside contents including $Rb_1$, $Rb_2$, Rc, Rd, Re, Rf, $Rg_1$, and $Rg_2$ in whole root of 3-year-old ginseng did not showed significant difference by greenhouse and traditional cultivation.

• Journal of Ginseng Research
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• 제11권1호
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• pp.10-16
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• 1987

인삼제품의 품질관리와 효능연구의 시료사용에 참고자료로 활용하기 위하여 인삼근의 부위별, 연근별 사포닌 함량 및 분포를 조사하였다. 한국인삼 6년근의 부위별 조성은 뇌두(rhizome) 4.1%, 동체(main root) 47.7%, 지근(lateral)의 비는 대략 1:1이었으나 지근부는 2:1로 피층이 많았다. 사포닌분포는 동체와 세근부가 29% 정도로 거의 같고, 지근부 34.6%로 가장 많았고, 뇌두는 7% 수준으로 가장 적었다. 그러나 단위 중량당 함량에서는 세근>퇴두>지근>동체의 순으로 뇌두가 높은 반면 동체는 가장 낮았다. 연근별 사포닌 변화는 2년근 4.89%, 6년근 7.98%로서 본포이식후 4년간 약 3% 증가에 그쳤으나 뿌리의 비대성장을 고려할 때 계속적으로 합성축적 되었으며 연간 증가율은 5년근이 43.8%로 가장 왕성한 성장기임을 나타내고 4년근 19.8%, 6년근 21.1%로 비슷했다. 따라서 5년에 뿌리의 생장과 사포닌 합성축적이 최고 수준에 도달했다가 6년째 부터 쇠퇴기로 됨을 알았다. 개별 사포닌의 함량변화는 전반적으로 증가하는 경향이나 PT계 사포닌보다는 PD계 사포닌의 증가가 보다 큰 경향이었다.

• Journal of Ginseng Research
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• 제20권1호
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• pp.65-71
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• 1996

This study was undertaken to evaluate the automatic decision-making on the grading of 6-year-old fresh ginseng (Panax ginseng C.A. Meyer) by an image analyzer. The best input method for the 6-year-old fresh ginseng was under condition of a low resolution (128u 128 pixel) and illumination direction from bottom to up (light box). It was possible to identify the main root, lateral root, and rhizome of fresh ginseng by application of OPEN process in a function of an image analyzer. Finally, we developed the grade decision-making programs, GinP-1. The fitness rates for the fresh ginseng standards which were classified by experts were 94.6, 80.6, 81.5, and 100.0% for 1st, 2nd, 3rd, and 4th grade of fresh ginseng, respectively, and the total time of decision-making was about 4.3 seconds per one root. The decision-making time was reduced to 0.8 seconds per one root by enhancemeat of the Image analyzer, which was tested by the technical company of the image analyzer,'Carl Zeiss (Germany). As a result of this study, the automatic decision-making on the grade of fresh gin send by image analyzer seems to have high possibility.

• 한국작물학회지
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• 제49권5호
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• pp.389-393
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• 2004

This study was carried out to investigate the difference of growth characteristics, yield and extract content between upland and paddy ginseng cultured with 4­year-old ginseng in 2003. Although upland ginseng showed larger variation in yield than that of paddy ginseng, the average of it was greater than that of paddy ginseng because it showed better growth of aerial part and higher survival rate than that of paddy ginseng. Moisture content of fresh root was $71.8\%\;(68.5\~73.1\%),\;and\;72.7\%\;(70.2 \~74.9\%)$ on average in upland and paddy ginseng, respectively. Paddy ginseng showed higher hardness in taproot, and higher rate of rusty colored root than that of upland ginseng. The ratio of taproot dry weight in upland ginseng was smaller than that of paddy ginseng, while that of lateral root was larger in upland ginseng. Ratio of marketable root (>60g) to total harvested roots was $13.7\%\;(0.82\~8.0\%)\;and\;7.7\%\;(1.6\~12.6\%)$ in upland and paddy ginseng, respectively. Extract content did not show distinct difference between upland and paddy ginseng, but it showed large variation from $16.1\;to\;25.1\%$ in taproot, and from $24.2\;to\;32.5\%$ in lateral root depanding on the ginseng field examined.

• Journal of Plant Biology
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• 제31권3호
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• pp.169-185
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• 1988

Vessel elements in lateral root, tap root, transition region, stem and mid vein of 1-year old, 3-year old and 5-year old ginseng (Panax ginseng C.A. Meyer) are studied with light microscope to clarify the distribution and differentiation of several kinds of vessel elements. Vessel elements are classified into five types such as ring vessel, spiral vessel, scalariform vessel, reticulate vessel and pitted vessel according to the secondary thickenings of cell wall. All of the five types are not observed in each organ, but diverse kinds of vessels are present in stem and mid vein compared with the underground organs such as tap root and lateral root. The length of vessel elements is longest (680$\mu$m) in stem and shortest (143$\mu$m) in tap root. The diameter of vessel elements is 19.0$\mu$m in tap root and the angle of perforation plate comes under 22$^{\circ}$-60$^{\circ}$. The degree of differentiation of vessel elements according to the length, diameter and angle of perforation plate of vessel elements is highest in tap root regardless of the age of ginseng. Three types of perforation plate such as scalariform, intermediate type of simple and scalariform, and simple perforation plate are observed. The vascular tracheids are characteristically observed in mid vein of 1-year old ginseng, and in transition region of 3 and 5-year old ginseng.