• 제목/요약/키워드: ginkgo leaves

검색결과 108건 처리시간 0.027초

은행잎 유래 살충성분 bilobalide와 그 분해물의 살충활성 (Insecticidal Activities of Bilobalide from Ginkgo biloba Leaves and its Derivatives)

  • 양은영;홍수명;안용준;권오경
    • 농약과학회지
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    • 제5권1호
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    • pp.24-29
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    • 2001
  • 은행잎함유 살충성분인 bilobalide의 활성부위를 조사하기 위해 bilobalide를 가수분해 및 산화를 시킨 후 구조를 확인하고 살충활성을 검정하였다. 분해물의 분석 결과, 염기에 의한 가수분해물은 cyclopentenone 계열의 화합물로 밝혀졌고, 산화물은 trilactone 형태를 유지한 sesquiterpene 구조로써 dehydration된 형태로 추정되었다. 분해물의 살충활성을 검정한 결과, 살충력의 크기는 bilobalide, monoacetyl 유도체, ginkgolide C, 산화분해물, diacetyl 유도체, 가수분해물의 순으로 감소하여 bilobalide의 살충력을 나타내는데에는 trilactone의 구조를 유지하는 것이 중요한 것으로 나타났다.

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Action mechanism of estrogen potentials of Ginko biloba extracts and its major components in human breast cancer cell

  • Kim, Yun-Hee;Oh, Seung-Min;Lee, Hee-Sung;Chung, Kyu-Hyuck
    • 대한약학회:학술대회논문집
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    • 대한약학회 2003년도 Proceedings of the Convention of the Pharmaceutical Society of Korea Vol.1
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    • pp.166.2-167
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    • 2003
  • The important biological activities of estrogen were reproduction and physiological processes in a number of tissues, including liver, bone, brain, blood vessels, adipose tissue and so on. The regulation of estrogen level is important a prevention of estrogen-related disease. Ginkgo biloba extracts (GSE) are extracted from leaves of the Ginkgo biloba tree. GSE contains 24% phytoestrogen, which are kaempferol, quercetin, and isorhamnetin. (omitted)

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Cytochemical Evidence on Seasonal Variation of Peroxidase Activities in Cambial Region of Pinus densiflora, Ginkgo biloba, and Populus alba

  • Wi, Seung-Gon;Lee, Kwang-Ho;Kim, Yoon-Soo
    • Journal of the Korean Wood Science and Technology
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    • 제28권4호
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    • pp.17-24
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    • 2000
  • The peroxidase activity was localized cytochemically to get an insight into its precise function in lignin biosynthesis. In this work, cerium chloride ($CeCl_3$) was used as a trapping agent for hydrogen peroxide ($H_2O_2$) generated from peroxidase. Seasonal variation of peroxidase activities in cambial region of Populus, Pinus, and Ginkgo was investigated at subcellular levels. Under transmission electron microscopy, electron dense deposits of cerium perhydroxide formed by reaction with $H_2O_2$ were observed in cambium and its immediate derivatives. The staining with $CeCl_3$ in cambium varied with growth seasons. The strongest $H_2O_2$ accumulation, regardless of tree species, appeared in May. Staining pattern of $CeCl_3$ in the cambium of poplar indicated that the production of peroxidase started in March before the opening of buds and reached the highest in May and then declined in August. Ginkgo and Pinus showed relatively late generation of $H_2O_2$ production when compared with Populus. Although Ginkgo and Pinus are classified into gymnosperms, however, the generation of peroxidase production and its duration was different from each other. Little staining appeared in all the tree samples collected in September before falling the leaves.

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Screening of Antimicrobial Activity against Enterohemorrhagic escherichia coli 0157 : H7 from Plants in Korea

  • Park, One-Kyun;Insun Joo;Kim, Ki-Hyun;Sung, Chang-Keun
    • Preventive Nutrition and Food Science
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    • 제3권4호
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    • pp.324-328
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    • 1998
  • We screened the methanol extracts from 133 plant species growing in Korea for antimicrobial activity against enterohemorrhagic Escherichia coli 0157 : H7. Those are selected from three plant grouping ; traditional medicinal herbs, edible plants, and flowers. They were tested by disk diffusion assay. From evaluation of the inhibition zone diameter of microbial growth, we found that the flower extract of Rhododendron Schilpenbachii Max had the most significant antimicrobial activity against this bacteria. Extracts from most of the vegetables and plants did not show antimicrobial activity except for the leaves of Ginkgo biloba L. and seeds of Prunus Dallicina L. did not show antimicrobial activity except for the leaves of Ginkgo biloba L. and the seeds of Prunus sallicina L.

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3종의 식물 추출물에 의한 백점충의 in vitro 구제효과 (In vitro eliminative effects three sorts of herbal extracts of against Ichthyophthirius multifiliis)

  • 김진도;김이청;정승희;서정수;박성우
    • 한국어병학회지
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    • 제24권3호
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    • pp.289-295
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    • 2011
  • 3종의 식물성 추출물 및 포르말린의 시험관 내에서의 백점충에 대한 살충효과를 조사하였다. 복합생약제는 500배 희석액에서 1시간 이내에 충을 완전히 죽일수 있었으나, 여지초발효액은 100배의 희석액에서 1시간 이내에 모든 충을 죽일 수 있었다. 은행잎 추출물은 5,000배의 희석액으로 1시간 이내에 모든 충을 죽일 수 있었다. 대조약제로서 포르말린은 100ppm의 농도로 1시간 이내에 충을 모두 죽일 수 있었다. 이 결과로 은행잎 추출물이 살충효과가 가장 좋았다. 충에의 밀도에 따른 살충효과의 차이는 없었다. 또한 은행잎 추출물의 5,000배 희석액으로 2시간 동안 약욕한 후의 어류의 아가미 조직에 있어서 변화가 없었다.

교통량 과밀 도로주변의 토양과 가로수, 대기중 Pb, Cu, Zn 중금속 농도와 그 상관성에 관한 연구 (Pb, Cu, Zn Contaminants and Their Correlation of Soil, Leave and Bark of Ginkgo. B and Ambient Air Adjacent to a Heavy Traffic Road Side)

  • 박기학
    • 한국환경보건학회지
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    • 제18권2호
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    • pp.19-25
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    • 1992
  • The study was carried out to investigate the correlation between the heavy metals emitted by the motor vehicles with the heavy traffic road side environment (soil, leave, bark, ambient air). The Pb, Cu, Zn contents in road side soil sand leaves, barks from Ginkgo, biloba and ambient air adjacent to the heavy traffic road side from June to August, 1992 Suwon city were analyzed by Atomic absorption spectrometry and Inductively coupled plasma emission spectrophotometry. The results were as follows: 1) The high levels of heavy metals concentration were Pb, at city-terminal in soil (186 $\mu$g/g), Cu, at city-terminal in soil (221 $\mu$g/g), Zn, at city-terminal in ambient air (252 $\mu$g/m$^{3}$). 2) The low leves of heavy metals concentration were Pb, at North-gate in ambient air (1.65$\mu$g/m$^{3}$), Cu, at North-gate in ambient air (4 $\mu$g/m$^{3}$), Zn, at North-gate in ambient air (15.31$\mu$g/m$^{3}$). 3) The regional distribution of Pb, Cu, Zn in road side soils, leaves and barks from Ginkgo, biloba, ambient air show high levels in turn, city4erminal, Guan Sean Dong, South gate, North gate. 4) The concentration of heavy metals (Pb, Cu, Zn) in soils, leaves, barks, ambient air was highly correlated with the traffic volume of the sampling sites (r=0.64~0.96). To conclude that the high levels of Pb, Cu, Zn contaminations were positively related to motor vehicles-borne pollutants and road side soils, trees, ambient air adjacent to a high density building area with low road coverage and heavy traffic volume were reflected strongly by the hazardous pollutants emitted by motor vehicles.

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은행잎에서 분리한 Polyphenol Oxidase의 정제 및 특성 (Purificaton and Some Properties of Polyphenol Oxidase from Ginko biloba Leaves)

  • 설지연;박수선;김안근
    • 생약학회지
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    • 제30권3호
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    • pp.306-313
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    • 1999
  • Polyphenol Oxidase(PPO) was purified from an extract of Ginkgo biloba leaves by ammonium sulfate fractionation followed by sephadex G-150 column chromatography, which resulted in a 18-fold increase in specific activity. The enzyme was most active at pH 8.5 and the temperature optimum for the PPO catechol oxidation reaction was $45^{\circ}C$. Heat inactivation studies showed that heating for 7, 9 and 48 min, at 80, 70 and $60^{\circ}C$ respectively caused a 50% loss in enzymatic activity and that the enzyme was completely inactivated after heat treatment at $90^{\circ}C$ for 60 min. Km values of the PPO for catechol, hydroquinone and 4-methylcatechol derived from Lineweaver-Burk plots were $6.06\;{\times}\;10^{-4}M,\;1.02\;{\times}\;10^{-3}M,\;1.41\;{\times}\;10^{-3}M$ respectively. Of the substrates tested, 4-methylcatechol was oxidized most readily and the enzyme did not oxidize monophenols. The enzyme datalyzed browning reaction was completely inhibited in the presence of reducing reagents, namely ascorbic acid, cysteine, glutathione, 2-mercaptoethanol, potassium metabisulfite at 0.5 mM level. Sodium chloride showed very little inhibition effect on Ginkgo biloba leaves PPO. Lineweaver-Burk analysis of inhibition data revealed that the inhibition by cysteine, 2-mercaptoethanol, potassium cyanide was competitive with ki values of $1.1\;{\times}\;10^{-5}M,\;2.4\;{\times}\;10^{-5}M,\;8\;{\times}\;10^{-5}M$, respectively. Among the divalent cations, $Cu^{2+}ion$ was a strong activator on PPO and $Mn^{2+}ion$ was little or no effect on PPO activity $Ni^{2+}ion$ was an inhibitor on PPO.

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은행나무와 곰솔에 처리된 인공산성비에 의한 잎의 형태변화 (Effect of simulated Acid rain on Foliar Structural of Changes of Ginkgo biloba and Pinus thunbergii)

  • 소웅영
    • Journal of Plant Biology
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    • 제38권1호
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    • pp.79-86
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    • 1995
  • 은행나무와 곰솔의 잎은 pH 3.2 이하의 인공산성비 처리구에서 괴사반점과 황화현상 및 조기노화 현상이 나타났다. 잎표면 구조의 관찰에서 은행나무 잎은 pH 2.4의 처리구에서 잎표면 왁스층의 침식이 심하게 일어난 반면 곰솔의 경우 pH 4.0 이하의 처리구에서 왁스층의 침식이 심하게 일어났다. 은행나무 잎은 pH 3.2 이하에서 표피세포와 해면조직에서 구조의 변화가 나타났으나 유관속조직은 정상적인 구조를 나타냈다. 엽육세포의 크기는 산도가 높아질수록 작아지고 세포간극이 커졌다. 곰솔의 잎은 표피세포보다 엽육조직과 유관속조직의 피해가 현저하였다. 은행나무의 기공의 크기와 모양은 인공산성비의 영향을 받지 않았으나 기공지수와 기공의 공극 크기는 높은 산도에서 증가하였다. 또한 인공산성비가 처리된 은행나무와 곰솔 잎의 기공들은 열려 있었다.

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은행잎의 Flavonoid 성분에 관한 연구 (Flavonoids from the Leaves of Ginkgo biloba)

  • 강삼식;김주선;곽의종;김기협
    • 생약학회지
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    • 제21권2호
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    • pp.111-120
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    • 1990
  • Five biflavones and sevenflavonolglycosideswereisolatedfromtheleaves of Ginkgo biloba. They were sciadopitysin(1), ginkgetin(2), isoginkgetin(3), bilobetin(4), amentoflavone(5), kaempferol 3-O-[$6'-O-{\rho}-coumaroyl-{\beta}-_D-glucopyranosyl(1{\rightarrow}2)-{\alpha}-_Lrhamnopyranoside$](6), quercetin 3-O-[$6'-O-{\rho}-coumaroyl-{\beta}-_D-glucopyranosyl(1{\rightarrow}2)-{\alpha}-_Lrhamnopyranoside$](8), rutinosides of kaempferol(7), isorhamnetin(9), quercetin(10), laricitrin(11), and kaempferol 3-O-($2',6'-{\alpha}-_L-dirhamnopyranosyl-{\beta}-_{D}-glucopyranoside$)(12). The structures were established by spectroscopic and chemical methods.

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