• Restorative Dentistry and Endodontics
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• 제42권4호
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• pp.316-323
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• 2017

Objectives: This study compared the amount of apically extruded bacteria during the glide-path preparation by using multi-file and single-file glide-path establishing nickel-titanium (NiTi) rotary systems. Materials and Methods: Sixty mandibular first molar teeth were used to prepare the test apparatus. They were decoronated, blocked into glass vials, sterilized in ethylene oxide gas, infected with a pure culture of Enterococcus faecalis, randomly assigned to 5 experimental groups, and then prepared using manual stainless-steel files (group KF) and glide-path establishing NiTi rotary files (group PF with PathFiles, group GF with G-Files, group PG with ProGlider, and group OG with One G). At the end of canal preparation, 0.01 mL NaCl solution was taken from the experimental vials. The suspension was plated on brain heart infusion agar and colonies of bacteria were counted, and the results were given as number of colony-forming units (CFU). Results: The manual instrumentation technique tested in group KF extruded the highest number of bacteria compared to the other 4 groups (p < 0.05). The 4 groups using rotary glide-path establishing instruments extruded similar amounts of bacteria. Conclusions: All glide-path establishment instrument systems tested caused a measurable apical extrusion of bacteria. The manual glide-path preparation showed the highest number of bacteria extruded compared to the other NiTi glide-path establishing instruments.

• Asian-Australasian Journal of Animal Sciences
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• 제14권7호
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• pp.986-993
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• 2001

In balance experiments with rats either 25 or 50% of the casein protein in the control diet was replaced with one of the following eight sun-dried tropical biomass products: water spinach plants (WS) (Ipomoea aquatica Forsk), leucaena leaves (LL) (Leuceana leucocephala), duckweed plants (DW) (Lemma minor L.), groundnut foliage (OF) (Arachis hypogaea L.), trichantera leaves (Tric) (Trichantera gigantea), indicago leaves (Ind) (Indigofera hirsuta), mungbean foliage (Mb) (Phaseolus aureus), and cassava leaves (CL) (Manihot esculenta Crantz). The experiment included 102 rats with six individuals per treatment group. In three of the 16 biomass treatment groups, feed intake and weight gain of the rats were unacceptably low, and therefore they were excluded from the statistical evaluation, The crude protein (CP) content of the biomass products varied between 20.9% (Tric) and 33.2% (DW), whereas the content of NDF varied between 18.5% (Ind) and 32.2% (DW) of dry matter (DM). The total content of essential amino acids (g/16 g N) was comparable with that of alfalfa meal, except for GF and Tric, which were inferior. Between plant species, differences in dietary digestibility of organic matter (dOM) and CP (dCP) were observed (p<0.001). Also, the replacement level negatively influenced dOM and dCP (p<0.001). The lowest values for dOM (p<0.001) were observed for diets including biomass products with the highest content of NDF (OF, Tric, Mb, LL). Digestibility of CP was negatively affected by level of protein replacement. Significant (p<0.001) differences were found in N-retention and biological value among diets with different biomass products. The most favourable overall results were obtained for DW, WS and CL. The main factors affecting the nutritive value of the diets tested were their NDF content, dCP and AA profile of the biomass. Also antinutritive component(s) may have influenced the process of digestion and metabolism of some of the biomass products.

• 한국농업기계학회:학술대회논문집
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• 한국농업기계학회 2017년도 춘계공동학술대회
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• pp.99-99
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• 2017

국내 콩의 소비량은 계속적으로 증가하고 있다. 재배 면적은 1995년 10,000ha에서 2013년 8,000ha까지 계속적으로 감소하였으며, 수입은 2009년 644.9백만 달러에서 2013년 883.3백만 달러까지 계속적인 증가 추세에 있다. 건조는 품질 손상을 최소화 및 수분을 제거하는 공정이다. 모든 곡물에는 건조 조건에 따라서 품질이 크게 변화하며, 건조 과정을 해석하여 해당 곡물의 적정 건조 조건을 구명하여 품질을 유지하는 위해서는 중요하다(Keum, 등 1977). 얇은 두께의 곡물층을 단층으로 하여 건조속도 및 건조공기의 조건을 나타내는 것을 박층(Thin layer)건조모델이라 한다. 박층건조모델을 계속적으로 쌓은 모델을 후층(Deep layer)모델이라 하며, 건조기 개발 및 건조조건 구명에 가장 기본이 된다(Basunia and Abe, 1998). 그러나 해외에서 만들어진 콩 박층건조 모델은 국내의 검은콩과 물성이 상이하여 건조조건이 다르게 설정 되어야 한다. 따라서, 본 연구목적은 건조온도 2수준, 상대습도 3수준에 대하여 검은 콩의 건조속도 측정하고, 곡물건조방정식으로 많이 이용되고 있는 4개의 건조방정식에 대하여 모델 적합성을 검정하여 검은콩 건조에 적합한 박층건조모델 결정하는데 있다. 공시재료는 2015년 강원도지역에서 생산된 검은콩을 실험에 사용하였으며, 초기 함수율은 14.7%,w.b.였다. 박층 건조공기 조성은 공기조화장치(MTH4100, SANYO, UK)를 사용하여 조성하였으며, 송풍기에 의해 공기충만실을 통과하여 건조 층을 통과하도록 구성하였다. 목표 평형함수율에 도달하면 실험을 종료하였다. 건조 층의 초기중량 및 경시적 변화는 전자저울(GF-4000, AND, Japan)을 이용하여 10분 간격 측정하여 저장하였다. 적정 박층건조모델을 결정하기 위하여 기존의 곡물건조방정식 중 널리 사용되고 있는 4개(Lewis, 1921; Page, 1949; Thompson, 1967; Moisture diffusion, 1975)의 건조모델을 이용하여 비교 검증하였다. 건조 초기 2시간 까지는 급격히 감소하다가 건조속도에 영향을 미치는 반건조시간(MR=0.5) 이후에는 완만하게 건조되는 것으로 나타났고 건조조건으로 건조온도 및 상대습도 모두의 영향이 미치는 것으로 나타났다. 4가지 건조 모델과 비교한 결과 Thompson 모델이 전체 건조 영역에서 비교적 잘 일치하였다.

• 한국정보과학회논문지:시스템및이론
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• 제30권1호
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• pp.33-49
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• 2003

인터넷의 확산으로 대용량 멀티미디어 데이타에 대한 요구가 증가하고 있으며 이를 효율적으로 관리하기 위한 스토리지에 대찬 연구가 진행되고 있다. 기하급수적으로 늘어나는 스토리지에 대한 요구를 해결하기 위해서 제시된 방법중의 하나가 공유디스크 환경을 제공하는 SAN(Storage Area Network)이다. SAN은 fibre channel이라는 고속 전송망을 이용해서 고속의 저장장치를 위한 네트워크를 구성한 것이다. 하지만 저장장치 네트워크의 구성만으로는 스토리지에 대한 요구는 해결하였지만 이를 사용자에게 제공하기 위한 공유디스크 환경에서의 파일시스템에 대한 연구는 미진하다. 특히 기존에 제시된 로컬 파일 시스템, 분산 파일시스템에서는 공유디스크환경에 적합하지 않으며 대용량 스토리지에 적합한 메타 데이터 구조 측면에서의 설계는 부족한 실정이다. 이를 해결하기 위해 본 논문에서는 공유디스크 환경에 적합한 메타 데이타 구조를 설계 및 구현하여 대용량 스토리지에 적합한 공유디스크 파일 시스템을 제시한다. 구현한 공유디스크 파일시스템은 SAN fabric에 참여하는 호스트들 사이의 균형적인 할당 블록을 주기 위한 파일시스템 레이아웃과 비트맵 관리기법, 대용량 파일을 위한 효율적인 익스텐트 기반의 SEMI FLAT구조를 제안하였으면, 대용량의 디렉토리를 사용할 수 있게 확장해싱을 이용한 2단계 디랙토리 관리 구조를 설계 및 구현하였다. 또한 리눅스 커널 상에서 제시한 메타 데이타 구조에 대한 구현에 필요한 구조 정보와 알고리즘을 제시하였으며. 성능의 우수성을 보이기 위해 리눅스 환경의 대표적인 파일 시스템인 EXT2, 공유디스크 환경의 GFS와의 성능을 파일 생성, 디렉토리 생성, I/O횟수 측면에서 비교하였다.

• 대한치과보철학회지
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• 제42권2호
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• pp.175-191
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• 2004

Statement of problem: Platelet-rich plasma(PRP) is well known to be very effective method to stimulate and accelerate the healing of bone and soft tissue. However, there are few reports which deal with the mechanisms of the PRP on the activation of the osteoblasts. Purpose: This study was aimed to investigate the effect of growth factors in PRP on the activity of osteoblasts. Material and method: To evaluate the effect on human, human osteoblast cell line was cultured. PRP was extracted from the blood of a healthy volunteer. Using the recombinant growth factors of PDGF, $TGFT-\beta$, IGF-1, bFGF which are mainly found at bone matrix and their neutralizing antibody, the effect of PRP on the attachment and proliferation of osteoblasts was evaluated. To evaluate the autocrine and paracrine effects, conditioned media(CM) of PRP was made and compared with PRP. By the western blot analysis, the expression of growth factors in PRP, CM was examined. Cell morphology was compared by the light microscope. Results : 1) The effects of CM on osteoblast were similar to the effects of PRP. 2) PRP, CM, recombinant $TGF-\beta$, bFGF, IGF-1 showed significantly higher cellular attachment than control(p<0.05) in the cell attachment assay. In the cell proliferation assay, PRP, CM, recombinant $TGF-\beta$, IGF-1, bFGF, PDGF increased significantly cell proliferation(p<0.01). Among the recombinant growth factors, IGF-1 showed the highest cellular attachment and proliferation. 3) In the western blot assay, bFGF, IGF-1, PDGF weve equally expressed in PRP and CM. 4) The attachment of osteoblast cell decreased significantly after the addition of neutralizing antibody against $TGF-\beta$, IGF-1(p<0.05). In the cell proliferation assay, the addition of neutralizing antibody against $TGF-\beta$, bFGF, PDGF, IGF-1 decreased significantly the cellular proliferation(p<0.05). The amount of decreasing in the cell attachment and proliferation is the highest in at-lGF-1. 5) The cells in control group were flattened and elongated with a few cellular processes in the a light microscope. But, the cells appeared as spherical, plump cells with well developed cellular processes in experimental groups. The cells in PRP and CM had more prominent developed features than recombinant growth factor groups. Conclusions : These findings imply that PRP maximize the cellular activity in early healing period using the synergistic effect, autocrine, paracrine effects of growth factors and increase the rate and degree of bone formation.

• Biomolecules & Therapeutics
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• 제1권2호
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• pp.188-195
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• 1993

In order to understand the machanism of action and regulation of ${\beta}$-adrenergic receptor in terms of molecular level, the purification of receptor protein has a fundamental importance. Moreover, species differences among avian, amphibian and mammalian ${\beta}$-adrenergic receptors make it more important to purify mammalian ${\beta}$-adrenergic receptor. Because ${\beta}$-adrenergic receptor is an integral membrane protein, it must be solubilized from the membrane for the purification. The purpose of the present study was to solubilize and characterize the mammalian $\beta$-adrenergic receptor from guinea pig lung in quantities by more efficient and practical method eventually to purify receptor. Guinea pig lung membrane preparation was solubilized by sequential treatment of buffers containing low and high concentration of digitonin which are 0.2 and 1.2% respectively. About 50% of the total receptor pool was released by this double extraction procedure. The $\beta$-adrenoceptors in the digitonin extract were identified using the ${\beta}$-adrenergic antagonist, (-)-[$^3H$]-dihydroalprenolol ([$^3H$]DHA). The solubilized receptor retained all of the essential characteristics of membrane-bound receptor, namely saturability; stereoselectivity; high affinity to ${\beta}$-adrenergic drugs. For the measurement of soluble receptor activity, Sephadex G-50 chromatography method has been widely used. Inspite of its accuracy and wide acceptance, this technique employed troublesome column work which required long time to assay the activity of receptor. We employed another methods to measure receptor activity. When using 0.5% polyethylenimine pretreated GF/B glass fiber filter, filtration technique could be used to measure soluble receptor activity. This technique enabled us to reduce the total amount of time to assay by a factor of 4 as well as to detect soluble receptor. In the present study, we could establish more efficient and practical solubilization method of mammalian $\beta$-adrenergic receptor. The rapidity and high yield of this solubilization scheme, together with the favorable recovery of the receptor activity, are significant steps toward the ultimate purification of the mammalian $\beta$-adrenergic receptor. The result of this study together with more convenient purification method could provide large amount of purified receptor with ease for various research purposes.

• Journal of Chest Surgery
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• 제29권8호
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• pp.850-860
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• 1996

활성산소는 동물실험에서 심근 재관류손상의 중요기전으로 알려져 있으나 실제 임상상황에서의 역할은 아직도 논란이 많다. 본 연구에서는 냉혈 심마비 액을 사용한 심근보호법을 이용하여 관상동맥우회술 을 시행받는 환자들을 대상으로 하여 심근허혈후 재관류시 활성산소에 의한 심근의 손상 정도 및 활성 산소 방어효소계의 변동과 그 기전을 규명하고자 하였다. 관상동맥우회술을 받는 환자(n=10)를 대상으로 하여 관상정 맥동 환류혈액 에서 상행대동맥 차단 전과 재관류 20분 후에 lactate dehydrogenase(LDH), creatinG phosphokinase MB 분획(CK-MB)과 malondialdehyde(MDA)의 농도를 측정하였으며 또한 같은 시각에 심근의 superoxide dismutase(SOD), catalase, glutathione peroxidase(GSHPX), glutathione reductase(GSSGRd) 그BT고 glucose 6- phosphate dehydrogenase(GGPDH)의 활성도를 측정하였다 관상정 맥동혈에서의 LDH(268 $\pm$40.3 to 448 $\pm$ 84.9 ml plasma)와 CK-MB(4.50$\pm$ 2.33 to 27.1$\pm$13.5 Ulml plasma)의 활성도 그리고 MDA(5.87$\pm$2.02 to 10.5$\pm$2.23 nmol/ml plsma)의 양은 상행대동맥 차단 전에 비하여 재관류 후에 현저히 증가하였으 \ulcorner심근의 SOD(13.5$\pm$4.04 to 20.7$\pm$8.56 mg protein), GSHPX(279 $\pm$)7.2 to 325$\pm$51.4 mU/mg protein) 그리고 GSSCRd(97.2$\pm$15.9 to 122 $\pm$25.1 m2/mg protein)의 활성도도 재관류후에 현저히 증가하였다 반면 심근의 catalase와GSPDH의 활성도는의미있는 변화가 없었다 한편 SOD에 대한 Western blot결과 Cu, Zn-SOD의 양이 현저하게 증가되었음을 관찰 하였다. 이상의 결과들로 관상동맥우회술시 상행대동맥차단에 따른 심근허혈후 재관류에 의하여 활성산소에 의한 산화성 심근손상이 일어나지만 동시에 활성산소 방어효소계의 활성 또한 증가됨으로써 심근손상 의 정도가 약화되었을 가능성을 추정할 수 있으며 이러한 활성산소 방어효소의 활성증가는효소단백의 광합성 증가에 의한 것으로 여겨진다.

• The Korean Journal of Physiology and Pharmacology
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• 제14권1호
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• pp.21-28
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• 2010

Phenolic compounds affect intracellular free $Ca^{2+}$ concentration ($[Ca^{2+}]_i$) signaling. The study examined whether the simple phenolic compound octyl gallate affects ATP-induced $Ca^{2+}$ signaling in PC12 cells using fura-2-based digital $Ca^{2+}$ imaging and whole-cell patch clamping. Treatment with ATP ($100\;{\mu}M$) for 90 s induced increases in $[Ca^{2+}]_i$ in PC12 cells. Pretreatment with octyl gallate (100 nM to $20\;{\mu}M$) for 10 min inhibited the ATP-induced $[Ca^{2+}]_i$ response in a concentration-dependent manner ($IC_{50}=2.84\;{\mu}M$). Treatment with octyl gallate ($3\;{\mu}M$) for 10 min significantly inhibited the ATP-induced response following the removal of extracellular $Ca^{2+}$ with nominally $Ca^{2+}$-free HEPES HBSS or depletion of intracellular $Ca^{2+}$ stores with thapsigargin ($1\;{\mu}M$). Treatment for 10 min with the L-type $Ca^{2+}$ channel antagonist nimodipine ($1\;{\mu}M$) significantly inhibited the ATP-induced $[Ca^{2+}]_i$ increase, and treatment with octyl gallate further inhibited the ATP-induced response. Treatment with octyl gallate significantly inhibited the $[Ca^{2+}]_i$ increase induced by 50 mM KCI. Pretreatment with protein kinase C inhibitors staurosporin (100 nM) and GF109203X (300 nM), or the tyrosine kinase inhibitor genistein ($50\;{\mu}M$) did not significantly affect the inhibitory effects of octyl gallate on the ATP-induced response. Treatment with octyl gallate markedly inhibited the ATP-induced currents. Therefore, we conclude that octyl gallate inhibits ATP-induced $[Ca^{2+}]_i$ increase in PC12 cells by inhibiting both non-selective P2X receptor-mediated influx of $Ca^{2+}$ from extracellular space and P2Y receptor-induced release of $Ca^{2+}$ from intracellular stores in protein kinase-independent manner. In addition, octyl gallate inhibits the ATP-induced $Ca^{2+}$ responses by inhibiting the secondary activation of voltage-gated $Ca^{2+}$ channels.

• 한국의류학회지
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• 제13권3호
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• pp.259-267
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• 1989

In this paper, the fabric specimen undergoes repeated laundering under given condition. After this cyclic laundering was applied, the crease recoveries of the specimen were measured using shirley crease revovery tester in order to evaluate the effect of factors at given condition during crease deformation. 5 samples of grey plain cloth were desized, alkali-scoured, bleached, whased with water, and air-dried. All tests were made on samples preconditioned to $65\%\;RH\;and\;20^{\circ}C$. The experimental results were analysed statistically to relate crease recoveries and the properties of smaples, recovery periods (time) of crease. Furthermore, the crease recoveries of core-spun yarn woven fabrics were discussed in comparison with those values for $100\%$ combed cotton yarn woven fabric and $65\%$ polyester $35\%$ carded cotton blended yarn woven fabric. The results obtained are as follows; 1. Regardless of materials, remarkable decrease are observed in crease recoveries about 1-5 cycles of the repeated laundering, but slack decrease are observed in crease recoveries after 5 cycle of the re-peated laundering. 2. Crease recoveries ($\alpha$) of core-spun yarn woven fabrics are relate to recovery periods (t) of crease as follows; log$\alpha$=0.01415 log t+2.1168 ($r^2=0.94$) 3. Core-spun yarn woven fabrics were superior to $100\%$ combed cotton yarn woven fabrics and $65\%$ polyester $35\%$ carded cotton blended yarn woven fabric in crease recoveries. 4. Crease recoveries ($\alpha$) of core-spun yarn woven fabrics are relate to cover factor (CF), thickness (T) at pressure 0.5 $gf/cm^2$, weight (W) as follows; log$\alpha$=-0.3482 log CF-0.4924 log T-0.4727 W+2.4243 ($r^2=0.88$) 5. Crease recoveries ($\alpha$) of core-spun yarn woven fabrics are relate to 2HB/B, 2HB/W, $\sqrt[3]{B/W}$, WC/T which are concerning to formation of weared clothes and bending Iran formation behavior as follows: log $\alpha$=0.0091 2HB/B+0.4667 2HB/W+0.0185 $\sqrt[3]{B/W}$+0.0114 WC/T+1.8433 ($r^2=0.86$)

• 분석과학
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• 제16권2호
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• pp.102-109
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• 2003

오줌시료 중 미량 알루미늄을 혹연로 원자흡수 분광광도법 정량하기 위한 마이크로파삭힘 및 고체상 추출에 관하여 연구하였다. 시료에 질산과 과산화수소를 가하고 가정용 전자레인지로 유기물을 파괴시킨 다음 오븐에서 건조시킨다. 건조된 찌기를 황산으로 녹이고, 이를 8-hydroxyquinoline (Oxine, HQ)이 흡착된 XAD-4 수지의 칼럼에 통과시켜 Al(III)-8-hydroxyquinolinate 착물을 형성시켜 흡착시킨다. 칼럼으로부터 0.5 M 질산으로 용리한 Al(III)을 정량하였다. 이 실험과정에서 찌기를 녹이기 위한 산의 종류, 수지에 흡착시키는 HQ의 양, 녹인 시료용액의 pH, 칼럼에 고정된 착물의 용리를 위한 산의 종류와 농도 등의 실험조건을 검토하여 최적화 시켰다. 인공 오줌 시료를 바탕으로 최적화 실험을 하였으며 검정곡선을 작성하였다. 실제 시료 중 알루미늄을 정량하였고, 표준용액을 일정량 첨가한 시료에서 구한 회수율은 94~101% 이였다. 바탕 세기에 대한 표준 편차의 3배 세기에 해당하는 검출한계는 0.05 ng/mL 이었다. 이로서 본 방법이 오줌시료 중 알루미늄을 분리하여 정량하는데 응용될 수 있음을 알 수 있다.