• 한국자원식물학회지
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• 제28권4호
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• pp.533-540
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• 2015

Germination properties, leachate electrical conductivity (EC), and inorganic compound leaching were analyzed to ascertain the storage ability and change of physiological characteristics during storage of Hippophae rhamnoides seeds. Seeds were placed in an incubator at 25℃ and sown in different soil media (sand, vermiculite and horticultural substrate) after being stored for 6, 18 and 30 months at 2℃. All germination properties decreased in accordance to an increase of the seed storage period. Compared with the seed storage for 18 months, germination percentage (GP), germination performance index (GPI), and germination value (GV) of seeds stored for 30 months decreased by more than 50%. When the seeds were sown in different soil media in a greenhouse, those germination properties were similar to the seeds germinated in an incubator, and mean germination time, GPI and GV had a significant difference except GP among soil media. EC and inorganic ion concentration had a strong positive correlation with the seed storage period, but the ratios of inorganic ions from stored seeds revealed that K⁺/Mg²⁺ and Na⁺/Mg²⁺ were inversely correlated with the storage period.

• Journal of Forest and Environmental Science
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• 제29권4호
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• pp.282-291
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• 2013

Rapid loss in viability of neem (Azadirachta indica A. Juss.) seed is a major problem. Present effort was undertaken for developing a set pattern for assessing of viability and vigour in seed of various mother tree age of neem (Age I-06 years, Age II-15 years, Age III-25 years and Age IV->30 years old). Various viability test viz. triphenyle tetrazolium chloride test, electrical conductivity, excised embryo test, and germination test have been performed on seeds obtained from mother tree age classes. Inconsistency was observed with the TTC and EC test in germination of seed in laboratory as well as nursery. While various vigour tests viz. cold test, chemical stress test (methanol stress test), and accelerated ageing test alongwith ageing index, germination test (G%, MGT and GV) and various seedling growth parameters like seedling length (cm), number of leaves, collar diameter (cm), total biomass (g) alongwith mathematical indices i.e. vigour index, sturdiness quotient, volume index, quality index, root shoot ratio in nursery as well have been taken for study and showed better consistency. On the basis present study results of various viability and vigour test indicated that mother tree age class II performed better in comparison to others and it can be recommended for seed collection. Further it is also recommended that viability of neem seed may be assessed using various laboratory tests like excise embryo test and germination test (G%, MGT and GV) and vigour test may be taken preferably by cold germination test, chemical (methanol) stress test, accelerated ageing test in laboratory and germination alongwith various seedling growth parameters seedling length (cm), number of leaves, collar diameter (cm), total biomass (g) alongwith mathematical indices like Vigour Index, Sturdiness quotient, Volume Index, Quality index, root shoot ratio in nursery as discussed in this study.

• 한국자원식물학회지
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• 제22권6호
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• pp.528-534
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• 2009

The study of germination and seedling growth characteristics of Neem under different soil and environment conditions was undertaken. The seed germination started 8 days after sowing in commercial bed soil, whereas, delayed germination was observed in sandy-loam (15 days) and sandy (19 days) soil. The highest germination (73.33%) was observed in commercial bed soil in green house, whereas, the lowest germination was observed in sandy soil (16.67%) and sandy-loam soil (8.33%). The seeds in the open field (sandy soil) also showed poor (10%) germination. The mean number of germination seed/day (GD) and seed germination vigor rate (GV) both were highest in the commercial bed soil with 0.733% and 16.67% respectively in the green house, whereas sandy and sandy-loam soil in green house and open field (sandy soil) all showed much lower GD and GV values. The seedling characteristics of nursery revealed that the seedling grown in the growth chamber in commercial bed soil was significantly higher in all the parameters comparing to others grown in green house and open field. The growth was nearly 7 fold in the chamber compared to that of the green house nursery observed in three months old seedlings. Likewise, HPLC analysis revealed that the green house grown seedling contain higher quantity of pigments compare to the chamber grown seedlings. Among the soils used the commercial soil alone or in combination with sandy and sandy-loam soil in the ratio of 2:1:1 respectively with the temperature of $27{\pm}2^{\circ}C$ showed better for Neem nursery preparation.

• 한국자원식물학회지
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• 제25권1호
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• pp.132-141
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• 2012

본 연구는 기능성 작물, 사방용 수종, 토양개량 등 활용성이 뛰어난 갈매보리수나무의 유용 유전자원의 장기보존 및 실생번식에 도움을 주고자 종자의 발아 온도조건 및 전처리에 대한 발아특성을 구명하고자 실시되었다. 갈매보리수나무 종자는 $10{\sim}35^{\circ}C$의 온도조건에 치상한 결과, 15, $20^{\circ}C$에서 발아율이 가장 높았으며, 평균발아일수, 발아속도 및 발아치는 $25^{\circ}C$에서 가장 좋은 결과를 나타내었다. 또한 주요 발아온도를 예측하기 위하여 2차 및 선형 회귀분석모델을 이용하였는데, 발아율을 이용한 2차 회귀분석 모델에서는 기준온도 $0.6^{\circ}C$, 최대온도 $36.4^{\circ}C$, 적정온도 $18.5^{\circ}C$로 나타났으며, 발아가능 온도범위는 $35.8^{\circ}C$이었다. 발아속도를 이용한 선형 회귀분석모델에서는 기준온도 $8.3^{\circ}C$, 최대온도 $35.4^{\circ}C$, 적정온도 $25.3^{\circ}C$로 나타났으며, 발아가능 온도범위는 $27.2^{\circ}C$로 분석모델간 차이를 나타내었다. 갈매보리수나무 종자를 생리적 처리 방법인 예냉, 층적 및 priming을 이용하여 전처리한 후 발아특성을 조사한 결과, 발아율에서는 $CaCl_2$ 300, 400 mM priming 처리구에서 가장 높은 수치를 나타내었다. 평균발아일수는 층적 6, 8주 처리구에서 가장 짧게 나타났으나 대조구 보다 발아율이 낮은 것으로 보아 발아일수의 단축 보다는 발아의 종기 종료로 판단되었다. 발아속도 및 발아치의 경우 예냉 1, 2주 처리구에서 가장 높게 나타났으며 발아율 또한 $CaCl_2$ 300, 400 mM 처리구에 이어 높은 수치를 나타내어 유묘 생산시 양적 질적 측면을 고려했을 때 가장 적정한 조건으로 판단 되었다.