• BMB Reports
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• 제36권3호
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• pp.319-325
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• 2003

Changes in the concentrations of $\gamma$-aminobutyric acid (GABA), soluble calcium ions, glutamic acid, and the activity of glutamate decarboxylase (GAD) were investigated in non-germinated vs. germinated brown rice. Brown rice was germinated for 72 h by applying each of the following solutions: (1) distilled water, (2) 5 mM lactic acid, (3) 50 ppm chitosan in 5 mM lactic acid, (4) 5 mM glutamic acid, and (5) 50 ppm chitosan in 5 mM glutamic acid. GABA concentrations were enhanced in all of the germinated brown rice when compared to the non-germinated brown rice. The GABA concentration was highest in the chitosan/glutamic acid that germinated brown rice at 2,011 nmol/g fresh weight, which was 13 times higher than the GABA concentration in the non-germinated brown rice at 154 nmol/g fresh weight. The concentrations of glutamic acid were significantly decreased in all of the germinated rice, regardless of the germination solution. Soluble calcium and GAD were higher in the germinated brown rice with the chitosan/glutamic acid solution when compared to the rice that was germinated in the other solutions. GAD that was partially purified from germinated brown rice was stimulated about 3.6-fold by the addition of calmodulin in the presence of calcium. These data show that the germination of brown rice in a chitosan/glutamic acid solution can significantly increase GABA synthesis activity and the concentration of GABA.

• 동아시아식생활학회지
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• 제24권1호
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• pp.92-100
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• 2014

In this study, the physicochemical and sensory properties of glutinous rice kochujang added with germinated barley powder were periodically examined during storage at $20^{\circ}C$ for 40 days. The pH level of glutinous rice kochujang added with germinated barley powder increased gradually with a higher amount of germinated barley powder, whereas it gradually decreased during storage. On the other hand, acidity showed the opposite pattern. The salinity of samples decreased gradually with a higher amount of germinated barley powder. The L-, a and b-values of samples increased gradually with a higher amount of germinated barley powder, whereas they decreased gradually during storage. Amino nitrogen content of glutinouse rice kochujang increased gradually with a higher amount of germinated barley powder, whereas they increased gradually during storage. In the sensory evaluation, glutinous rice kochujang added with 5~10% germinated barley powder was superior in color, texture and overall preference. Therefore, addition of 5~10% germinated barley powder was optimum for improving kochujang quality.

• 한국식품영양학회지
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• 제23권4호
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• pp.511-515
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• 2010

It was conducted to assess GABA(${\gamma}$-aminobutyric acid) producing capacity and vitamin contents of germinated brown rice soaked in different soaking solutions. For germination, samples were soaked in 5% glutamic acid solution and 5% lactic acid solution as test group to investigate GABA producing capacity, and samples soaked in the solution without glutamic acid and lactic acid were set as control groups(rice and non-germinated brown rice). The GABA contents of the samples were $44.80\;{\mu}g/g$ for rice, $59.90\;{\mu}g/g$ for non-germinated brown rice, $146.70\;{\mu}g/g$ for germinated brown rice, $203.20\;{\mu}g/g$ for germinated brown rice soaked in glutamic acid solution and $222.5\;{\mu}g/g$ for germinated brown rice soaked in lactic acid solution, resulting in a significant difference(p<0.05). GABA producing capacity was enhanced by the addition of glutamic acid and lactic acid in the soaking solution for brown rice germination. The GABA contents of the germinated brown rice soaked in lactic acid solution greatly increased, along with increases in niacin and vitamin E contents without losing vitamin $B_1$. In conclusion, the addition of lactic acid in soaking solution is most suitable for germination of brown rice.

• 한국식품영양과학회지
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• 제39권2호
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• pp.274-280
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• 2010

발아 전과 후의 현미와 미강을 첨가한 밀가루 복합분으로 만들어진 국수의 품질 특성과 GABA 함량 및 항산화 활성의 변화를 조사하였다. 발아 전후의 현미와 미강을 각각 5, 10, 15% 첨가하여 국수를 제조하였다. 이렇게 제조된 국수는 조리 및 제면특성(cooking loss, swelling index, water holding capacity), 조리에 따른 GABA 함량과 항산화 성분(polyphenol) 및 활성(ABTS와 DPPH의 radical scavenging activities) 변화를 통해 기능성 국수로의 이용가능성을 알아보고자 하였다. 실험결과, 발아 후 rice bran의 첨가에 따른 cooking loss가 증가하는 것으로 관찰되었으며 반면에 swelling index에서는 차이를 나타내지 않았다. 발아 후 현미와 미강을 첨가한 조리 전후 국수에서 발아 전 현미와 미강을 첨가한 국수보다 높은 GABA 함량을 확인하였으며 발아에 따른 polyphenol 함량과 항산화 활성에서는 큰 변화가 없음을 확인할 수 있었다. 따라서 발아현미가 첨가된 국수는 밀가루 국수의 영양학적 가치와 항산화 기능을 향상시킬 수 있는 소재로써 이용 가능할 것으로 생각된다.

• 한국식품과학회지
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• 제42권6호
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• pp.688-692
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• 2010

The aim of this study was to investigate the effects of molecular weight and concentrations of chitosan on the germination of brown rice. Brown rice was germinated at $30^{\circ}C$ for three days in various chitosan solutions. The germination rate of the brown rice increased with increasing concentrations of chitosan solution, and was higher in the chitosan solution than in water. GABA content increased with increasing germination time and chitosan solution concentration. As the molecular weight of the chitosan decreased, germination rate and GABA content increased in the brown rice. The GABA content of germinated brown rice using low molecular weight chitosan A in a 100 ppm solution was 5145.5 nmole/g. This is approximately a five times higher value than that of the water-germinated brown rice. Texture properties were enhanced in all the germinated brown rice samples in chitosan solution compared to the brown rice germinated in water. These results indicate that chitosan solution treatment can increase germination rate and GABA synthesis activity in brown rice during germination, and can also improve the texture properties of brown rice.

• Applied Biological Chemistry
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• 제48권3호
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• pp.222-227
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• 2005

특수미인 거대배아미와 유색미의 발아처리에 의한 항돌연변이 활성의 변화를 평가하고자, 70% 에탄올 추출물이 in vitro에서 산화적 손상에 의한 DNA strand scission을 억제하는 효과와 함께 E. coli 및 V79 배양세포에 화학적으로 유발된 돌연변이에 대한 억제효과를 측정하였다. Mitomycin C로 유발된 돌연변이에 대한 억제활성을 E. coli에서 SOS chromotest로 조사한 결과, 활성은 발아유색미(40.4%) > 발아거대배아미(37.1%) > 무발아유색미(35.5%) > 발아현미(15.7%) > 무발아거대배아미(14.0%) > 무발아현미(0.8%)의 순서였다. Mitomycin C가 유도한 DNA strand scission에 대한 억제 효과는 무발아유색미 > 무발아거대배아미 > 발아유색미 > 발아현미 > 무발아현미 > 발아 거대배아미로 나타났다. V79 세포주에서 4-NQO로 유도된 6-TG 저항성 colony의 형성을 저해하는 활성을 지표로 항돌연변이 활성을 조사한 결과, 발아거대배아미(53.2%) > 무발아유색미(40.0%) > 무발아현미(21.2%) > 발아현미(14.4%) > 무발아거대배아미(0.23%)의 순서로 돌연변이를 저해하였는데, 발아유색미는 돌연변이를 촉진하는 것으로 나타났다(-69%).

• 한국작물학회지
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• 제63권3호
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• pp.219-228
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• 2018

Weedy rice (Oryza sativa) is a semi-wild rice grown in paddy fields and is more adapted to the environment than cultivated rice. Therefore, it is expected that the seeds of weedy rice might contain some chemicals related to its environmental adaptation, such as antioxidants, which may be useful for nutritional and medicinal purposes. This study was carried out to investigate the antioxidant activities of weedy rice compared to a Korean-bred rice cultivar, Sindongjin (SDJ), and to screen lines that show higher antioxidant activity in 199 accessions of weedy rice germplasm collected in Korea. DPPH (1,1-diphenyl-2-picrylhydrazyl) radical scavenging activity ranged from 31.0 to 91.7%, with an average of 82.5%, and 19 accessions that showed more than 91% antioxidant activity were selected. The 19 accessions were re-screened against non-germinated brown rice (BR) and germinated brown rice (GBR) using four assays, total phenol determination, DPPH radical scavenging, ABTS radical scavenging, and reducing power activities. The results showed that accession 'WD3' had the highest antioxidant capacity in both BR and GBR, suggesting that WD3 is a promising potential source of antioxidants and could be developed as a potentially functional substance material.

• 한국식품조리과학회지
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• 제28권6호
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• pp.813-820
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• 2012

To develop the high quality gluten-free rice products with health functionality and desirable texture with moistness, the physicochemical properties of extruded rice flours prepared from the mixture of germinated brown and white rices were investigated. The domestic organic Samgwangbyeo was used to make white and germinated brown rices. White rice (WR) was dried after soaked for 6 h at $15{\pm}3^{\circ}C$ and mixed with germinated brown rice (GBR) with different mixing ratios (100:0, 75:25, 50:50, 25:75, 0:100). The operating conditions of twin screw extruder were 250 rpm of screw speed, $120^{\circ}C$ of barrel temperature, and 25% moisture content of rice flour. The ash, crude protein and crude lipid contents were significantly different (p<0.05) and those of extruded GBR were the highest values, but those of extruded WR were the lowest. The color difference of extruded WR based on white plate showed the lowest among them. The water binding capacity (334.16%), swelling power (8.83 g/g), solubility (33.13%), and total starch (79.50%) were the lowest in extruded GBR. The viscosities of all extruded rice flours by RVA were maintained during heating. The peak and total setback viscosities of extruded rice flours ranged 127-352 and 58.0-85.5 cP, respectively. The novel food biomaterial from germinated brown rice as well as white rice was developed by twin screw extruder. The extruded rice flours control the moistness to improve the texture and also have functional materials, dietary fiber, GABA, and ferulic acid, etc to increase quality of gluten free rice products.

• 한국식품과학회지
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• 제44권3호
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• pp.300-305
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• 2012

본 연구는 국내 주요 벼 품종들중 일부 품종들에 대하여 현미와 발아현미에 대한 품질 특성에 대해 비교 분석하였다. 발아율은 95.0% 이상이었으며 단백질 함량은 현미 미광이 9.85%로서 가장 높았다. 아밀로스 함량 분석결과 현미보다 발아현미에서 높았으며 알칼리 붕괴도(ADV)는 품종 및 발아에 따라 다양하게 나타났다. 아밀로펙틴은 단쇄비율이 높을수록 밥맛이 좋은 것으로 알려져 있는데 삼광벼가 현미와 발아현미에서 각각 다른 품종들에 비하여 유의적으로 높음을 알 수 있었다. RVA 측정결과, 호화온도는 거의 비슷한 온도($67.93-68.05^{\circ}C$)였으며 최고점도는 현미 삼광이 167.92 RVU로 가장 높은 수치를 보였다. 강하점도의 경우 발아현미 미광이 5.28 RVU로 가장 낮게 측정되어 가공시 안정도가 높을 것으로 사료되며 치반점도는 현미 미광이 14.34 RVU로서 조사되어진 품종들중 가장 높은 것으로 나타났다. 식감 측정결과 경도(hardness)는 발아현미 하이아미밥이 54.14로서 가장 낮은 값을 나타내었고 끈기(toughness)는 발아현미밥보다 현미밥에서 높았다. 찰기(stickiness)에서는 발아현미 삼광밥에서 51.45로서 가장 높은 값을 나타내었으며 부착성(adhesiveness)은 발아현미밥이 현미밥에 비하여 높은 값을 나타내었다. 따라서 본 연구에서는 주요 벼 품종들의 현미에 대하여 발아 전과 후의 품질 특성을 종합적으로 비교·분석한 결과, 식미가 우수한 최고품질 벼 품종인 삼광과 하이아미의 현미에서 발아 후 식감이 부드러워지고 찰기가 증대되어 밥맛이 향상되어 이는 발아현미 가공용으로 유망할 것으로 사료된다. 그리고 이와 같은 발아현미용 적합품종은 앞으로 국내 현미산업 활성화에 기여할 것으로 생각된다.

• 한국식품과학회지
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• 제29권1호
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• pp.101-106
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• 1997

식혜원료로의 활용가능성 검토를 위해 현미와 벼를 $15^{\circ}C$에서 2일간 침지한 후 $15,\;20,\;25,\;30^{\circ}C$에서 최대 10일간 발아시키면서 잎눈의 성장, ${\alpha}-amylase$의 활력, 추출물의 함량, 당도 및 점도를 관찰하였다. 발아온도가 오를수록 현미와 벼의 발아속도, ${\alpha}-amylase$의 활성 증가 속도, 추출물의 함량 및 그 당도 증가 속도 모두 빨라졌으며, 추출물의 점도는 발아일수 경과에 따라 먼저 감소하다가 그 후 증가하는 경향을 보였다. 발아 현미와 벼의 ${\alpha}-amylase$의 활력은 발아 보리보다 작았으나 추출물의 함량 및 그 당도는 큰 차이가 없었다. 식혜제조시 발아 현미나 벼를 보리 엿기름 대신 사용할 가능성은 없으나 발아 현미를 백미 대신 식혜 원료로 사용할 경우 현미 자체를 원료로 사용한 경우보다 당화속도도 빠르고 당화 후 현미의 물성도 백미처럼 부드러울 수 있으리라 예상되었다.