• 한국잠사곤충학회지
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• 제39권2호
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• pp.134-139
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• 1997

To elucidate genetical and biochemical characters of the white egg strains preserved in National Sericultural and Entomology Research Institute(NSERI), RDA of Korea, the genetic segregation ratios in egg colours were investigated by crossing test, and egg pigment precursors were also analyzed by paper chromatography and UV-spectrophotometer scanning. The result obtained by crossing test between the white egg strains and normal one illustrated that the most of white egg strains showed typical segregation ratio of white egg-2(w-2), while maternal inheritance which can be seen in white egg-1(w-1) was not found in any white egg strains. Paper chromatographic analysis showed that egg extracts of all the white egg strains contained 3OH-kynurenine, while kynurenine which is known to existed in white egg-1(w-1) could not be detected at all. From the results of these experiments, it was found that all the white egg strains preserved in NSERI were classified as the white egg-2(w-2) strain.

• 한국잠사곤충학회지
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• 제38권2호
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• pp.113-117
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• 1996

누에의 양배추에 대한 섭식성에 관한 유전양식을 밝히기 위하여 양배추에 대한 섭식성이 높은 Fb잠과 섭식율이 낮은 교잡원종들 간에 섭식성을 조사하였다. 양계통의 F1에서는 두 계통의 중간정도의 섭식성을 나타내었으며 정·역교잡간의 차이는 인정되지 않았다. F1, F2 및 BF1에서의 섭식율의 분포로보아 양배추 섭식인자는 불완전우성유전을 하며 상염색체상에 좌위하는 다수의 유전자에 의해 발현되는 것으로 사료된다.

• 한국식물생명공학회:학술대회논문집
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• 한국식물생명공학회 2005년도 추계학술대회 및 한일 식물생명공학 심포지엄
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• pp.361-366
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• 2005

Opium poppy (Papaver somniferum L.) is one of the most important medicinal plants, which is used as a sole commercial source of narcotic analgesic, morphine. The transformant of opium poppy we have established by infection of Rhizobium rhizogenes (formerly Agrobacterium rhizogenes) strain MAFF03-01724 showed aberrant morphology and altered opium alkaloid composition. The major alkaloid produced by this transformant was thebaine (16.3%, opium dry weight) instead of morphine. It is likely that this 'thebaine poppy' phenotype was caused by the integration of T-DNA(s) into the poppy genome DNA, and their inserted loci are of great interest. To gain an insight into the mechanism of nonnarcotic thebaine accumulation for the further approach toward the creation of 'codeine poppy' which produces codeine as a major alkaloid, the genetical and morphological analyses on the transformant was carried out. Here we report the results of the detailed analysis on the T-DNA inserted loci of T0 transfromant and the correlation between opium alkaloid composition and segregated T-DNA integration pattern in the self-pollinated T1 transformants.

• Journal of the korean society of oceanography
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• 제35권3호
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• pp.158-160
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• 2000

The relativity of four isolates of C. polykrikoides was determined by comparative sequence analysis based on direct sequencing of PCR amplified ribosomal DNA (the internal transcribed spacer region and the 5.8S rDNA). Sequence comparisons indicated that four isolates had the same nucleotide sites in the ITS regions, as well as a total of 585 nucleotide length and 100% homology. The molecular data revealed that C. polykrikoides in Korean coastal waters show no genetical difference.

• Asian-Australasian Journal of Animal Sciences
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• 제10권6호
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• pp.541-550
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• 1997

Anaerobic chytridiomycete fungi are now well recognized as one of the major components of rumen microflora. Since the discovery of anaerobic fungi, the knowledge upon their morphology and physiology has been accumulated. It is certain that they gave roles in ruminal fiber digestion, although their quantitative contribution to rumen digestion is still unclear. Their role in fiber digestion is complicated by the dietary factors and the interaction with other microorganisms. We aim at reviewing such information in this article. Considerable attention gas been paid to the polysaccharidase of these fungi. Analysis on the fungal genes encoding these enzymes has been performed in several laboratories. This article also covers the genetical analysis of fungal polysaccharidases.

• Asian-Australasian Journal of Animal Sciences
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• 제9권4호
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• pp.405-410
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• 1996

Phylogenetic analyses were carried out using four Indonesian native chicken breeds; Kampung, Bangkok, Pelung and Kedu. Gene frequencies of four blood group (A, B, D and E) and eight electrophoretic loci (akp, Akp-2, Es-1, Amy-1, Alb, Tf, Pas and Pa-1) were examined. Geographical and breed specific trends in the gene frequencies were not found in the local population of Kampung breed or in four native breeds. The values of average heterozygosity were estimated as 0.35-0.45. Genetic distances among the local populations of Kampung breed and other native breeds were comparatively small. In a cluster analysis, the Bangkok breed and Kampung E population showed distance from another cluster. The coefficient of gene differentiation for local populations of Kampung breed was estimated as 0.099.

• 한국자원식물학회지
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• 제10권2호
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• pp.200-210
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• 1997

The understanding on the plant genome is accelerated with the fast advance of molecular biological techniques. The molecular dissecting of the plant genome has made possible the precise genotyping the plants, which can be utilized for molecular breeding program. As well, the molecular cloning of genes interested can facilitate the process of gene transfer between intra-and inter-generic taxa. Moreover, the manipulation of the agronomically important QTL genes, which can be rarely performed by the conventional genetic methods, is also possible by the utilization of molecular markers. In addition to these genetical applications, molecular markers are useful in the areas of plant taxonomy and management of germplasm by fingerprinting analysis. This paper describes the theoretical aspects marker technologies and practical applications of each marker technique.

• 한국식물학회:학술대회논문집
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• 한국식물학회 1987년도 식물생명공학 심포지움 논문집 Proceedings of Symposia on Plant Biotechnology
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• pp.323-347
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• 1987

The R locus of maize in one of several genes that regulate the anthocyanin pigments throughout the body of the plant and seed. The R gene product may regulate pigment deposition by controlling the expression of the flavonoid biosynthetic gene pathway in a tissue-specific manner. To understand the basis for tissue specific regulation and allelic variation at R, the molecular study has been done by cloning a portion of the R complex by transposon tagging with Ac. R specific probe were cloned from the R-nj mutant induced by Ac insertion mutagenesis. From southern analysis of R-r complex using the R-nj probe, the structure of R-r was proposed that R-r containes the three elements, (P)(Q)(S). These elements may organize as the inversion triplication model which (S) sequence was inverted in relation to (P) and (Q). The R-sc derivated from R-mb or R-nj was cloned with R-nj probe, and molecular genetical data showed that R-sc containes tissue specific and tissue nonspecific area, and the sequencing of R-sc are progressed now.

• 사상체질의학회지
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• 제11권1호
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• pp.159-168
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• 1999

Purpose : Sasang Constitutional Medicine explains the pathology peculiar to constitution and suggests treatment for each constitution. In Sasang Constitutional Medicine hwnan beings are classified on four groups; Taeyangin, Taeumin, 5oyangin, Soeumin. These four constitution has their's own symptoms and treatments. In treatment, control of mind inclination, that is to say, moderation takes a very important role. But the study on heredity in Sasang Constitutional Medicine has not done not biological study but also statistical study. So we used several statistical methods and analyzed 163 samples. Methods : We implemented Fishers exact test for adjusting chi-squared test, kappa coefficient to estimate agreement of parent's and children's constitutions, and finally plotted bi-plot using correspondence analysis. Results : From Fisher's exact test result, we could know that parent's and children'S constitution's distribution had significant difference. In kappa coefficient, mother and daughter's estimated value produced highest result. In correspondence analysis we only plotted the case of mother and son for easy interpretation. Conclusion : In the study of heredity of SaSang constitution, we cannot know exactly the heredity of constitution in terms of biology or genetics. But this research can be helpful for further analysis, that is, a study of biological or genetical aspects. And we could conclude that in statistical aspects the heredity in SaSang constitution is meaningful.

• 대한수의학회지
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• 제32권4호
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• pp.597-603
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• 1992

To elucidate the molecular genetical properties of the canine parvoviruses isolated from the diseased puppies in the regions of Kyunggi and Chungnam provinces, the replicative form (RF) DNA of four field isolates were compared with those of two attenuated vaccine strains and a reference strains of CPV by restriction endonuclease analysis (REA). REA by Hinf I showed that three CPV isolates except CPV-V15 had an identical banding pattern with two vaccine strains, one standard strain and feline panleukopenia virus (FPLV). In CPV-V15 strain the fourth fragment of DNA with 800 bp was deleted. REA by Bgl II and Pst I indicated that CPV-V15 and FPLV had a bigger second fragment than those of the other strains of CPV. Meanwhile REA by Bam HI revealed that all the field isolates and vaccine strains used in this experiment showed similar banding patterns.