• 제목/요약/키워드: genetic marker

검색결과 1,072건 처리시간 0.031초

Simple Sequence Repeat (SSR) Marker를 이용한 토마토 품종 식별 (Use of Simple Sequence Repeat (SSR) Markers for Variety Identification of Tomato (Lycopersicon esculentum))

  • 권용삼;박은경;배경미;이승인;박순기;조일호
    • Journal of Plant Biotechnology
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    • 제33권4호
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    • pp.289-295
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    • 2006
  • 국내에서 유통되고 있는 토마토 품종의 판별 방법에 SSR marker의 이용 가능성에 대한 연구를 수행하여 얻어진 결과를 요약하면 다음과 같다. 토마토 28품종을 18개의 SSR marker를 이용하여 분석하였을 때 대립유전자의 수는 $2{\sim}9$개로 비교적 다양한 분포를 나타내었으며 전체 60개의 대립유전자가 분석되었다. PIC 값은 $0.476 {\sim}0.800$ 범위에 속하였으며 평균값은 0.607로 나타났다. SSR marker를 이용하여 작성된 토마토 28품종의 품종간 유전적 거리는 $0.35{\sim}0.97$의 범위로 나타났고, 유사도 지수 0.36을 기준으로 할 때 28개 품종은 체리형 토마토 그룹과 일반형 토마토 그룹으로 나눌 수 있었으며, 공시 품종 모두 SSR marker의 genotype에 의해 뚜렷이 구분되었다. 이 연구결과는 토마토의 품종식별에 기초 자료로 유용하게 이용될 수 있는 것으로 나타났다.

Discrimination of Lonicera japonica and Lonicera confusa using chemical analysis and genetic marker

  • Ryuk, Jin Ah;Lee, Hye Won;Ko, Byoung Seob
    • 대한본초학회지
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    • 제27권6호
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    • pp.15-21
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    • 2012
  • Objective : Lonicera japonica THUNB. a traditional herbal medicine, has been commonly used anti-inflammatory disease. It has been very complicated with respect to its sources on the market. The significant selection of medicine depends on its origin. However, it is difficult to discrimination criteria for confirming L. japonica authenticity using the senses. This study was performed to determine the discriminant analysis of L. japonica and L. confusa. Methods : The identification of L. japonica and L. confusa were performed by the classification and identification committee of the national center for standardization of herbal medicines. And we examined its differences using HPLC and genetic marker analysis. Results : The analytical pattern of High Performance Liquid Chromatography was determined from the corresponding peak curves ((E)-aldosecologanin, chlorogenic acid, luteolin 7-O-glucoside, sweroside). For L. japonica, additional unknown peaks were detected at 13.8 min, 20.6 min, and 36.9 min. And, we developed genetic marker using the the tRNA-Leu gene, trnL-trnF intergenic spacer and tRNA-Phe region of chloroplast DNA. By the method, 164 bp PCR product amplified from L. confusa was distinguished into L. japonica and L. confusa efficiently. Conclusion : Base on these results, two techniques provide effective approaches to distinguish L. japonica from L. confusa.

RFLP Analysis of the mtDNA COI Region in Four Abalone Species

  • Park, Choul-Ji;Kijima, Akihiro
    • Fisheries and Aquatic Sciences
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    • 제9권3호
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    • pp.101-106
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    • 2006
  • The cytochrome c oxidase subunit I (COI) gene region of mitochondrial DNA (mtDNA) was examined in four abalone species to estimate its utility as a genetic marker using restriction fragment length polymorphism (RFLP) analysis. The utility was evaluated in terms of genetic divergence and relationships among Haliotis discus hannai, H. rufescens, H. rubra, and H. midae in both hemispheres of the world. There was clear genetic divergence in the mtDNA COI region between all pairs of the four species. Moreover, relationships among the abalone species were reflected in their geographical distributions and morphological characteristics. Therefore, RFLP analysis of the mtDNA COI region is a suitable genetic marker for the estimation of genetic divergence and relationships among abalone species. However, it is not effective for the evaluation of genetic differences within abalone species.

Immunotherapeutic Approach for Better Management of Cancer - Role of IL-18

  • Kuppala, Manohar Babu;Syed, Sunayana Begum;Bandaru, Srinivas;Varre, Sreedevi;Akka, Jyothy;Mundulru, Hema Prasad
    • Asian Pacific Journal of Cancer Prevention
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    • 제13권11호
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    • pp.5353-5361
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    • 2012
  • Interleukin-18 (IL-18) is an immune-stimulatory cytokine with antitumor activity in preclinical models. It plays pivotal roles in linking inflammatory immune responses and tumor progression and is a useful candidate in gene therapy of lymphoma or lymphoid leukemia. A phase I study of recombinant human IL-18 (rhIL-18) in patients with advanced cancer concluded that rhIL-18 can be safely given in biologically active doses to patients with advanced cancer. Some viruses can induce the secretion of IL-18 for immune evasion. The individual cytokine activity might be potentiated or inhibited by combinations of cytokines. Here we focus on combinational effects of cytokines with IL-18 in cancer progression. IL-18 is an important non-invasive marker suspected of contributing to metastasis. Serum IL-18 may a useful biological marker as independent prognostic factor of survival. In this review we cover roles of IL-18 in immune evasion, metastasis and angiogenesis, applications for chemotherapy and prognostic or diagnostic significance.

Evaluation of Cofactor Markers for Controlling Genetic Background Noise in QTL Mapping

  • Lee, Chaeyoung;Wu, Xiaolin
    • Asian-Australasian Journal of Animal Sciences
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    • 제16권4호
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    • pp.473-480
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    • 2003
  • In order to control the genetic background noise in QTL mapping, cofactor markers were incorporated in single marker analysis (SMACO) and interval mapping (CIM). A simulation was performed to see how effective the cofactors were by the number of QTL, the number and the type of markers, and the marker spacing. The results of QTL mapping for the simulated data showed that the use of cofactors was slightly effective when detecting a single QTL. On the other hand, a considerable improvement was observed when dealing with more than one QTL. Genetic background noise was efficiently absorbed with linked markers rather than unlinked markers. Furthermore, the efficiency was different in QTL mapping depending on the type of linked markers. Well-chosen markers in both SMACO and CIM made the range of linkage position for a significant QTL narrow and the estimates of QTL effects accurate. Generally, 3 to 5 cofactors offered accurate results. Over-fitting was a problem with many regressor variables when the heritability was small. Various marker spacing from 4 to 20 cM did not change greatly the detection of multiple QTLs, but they were less efficient when the marker spacing exceeded 30 cM. Likelihood ratio increased with a large heritability, and the threshold heritability for QTL detection was between 0.30 and 0.05.

분자마커를 활용한 옥수수 육종 (Genetic Improvement of Maize by Marker-Assisted Breeding)

  • 김재윤;문준철;백성범;권영업;송기태;이병무
    • 한국작물학회지
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    • 제59권2호
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    • pp.109-127
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    • 2014
  • Maize is one of the most important food and feed crops in the world including Southeast Asia. In spite of numberous efforts with conventional breeding, the maize productions remain low and the loss of yields by drought and downy mildew are still severe in Asia. Genetic improvement of maize has been performed with molecular marker and genetic engineering. Because maize is one of the most widely studied crop for its own genome and has tremendous diversity and variant, maize is considered as a forefront crop in development and estimation of molecular markers for agricultural useful trait in genetics and breeding. Using QTL (Quantitative Trait Loci) and MAS (Marker Assisted Breeding), molecular breeders are able to accelerate the development of drought tolerance or downy mildew resistance maize genotype. The present paper overviews QTL/MAS approaches towards improvement of maize production against drought and downy mildew. We also discuss here the trends and importance of molecular marker and mapping population in maize breeding.

RAPD 표식자(標識者) 분석(分析)에 의한 사시나무속(屬) Leuce절(節) 포플러의 유연관계(類緣關係) (Genetic Relationships among the Poplars of Section Leuce (Genus Populus) revealed by RAPD Marker Analysis)

  • 홍강낙;현정오;홍용표
    • 한국산림과학회지
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    • 제87권2호
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    • pp.153-163
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    • 1998
  • 사시나무속(屬)(Genus Populus) Leuce절 수종중 우리나라에 식재되어 있는 사시나무, 수원사시나무, 은백양, 은사시나무와 인공교배종 수개 클론에 대한 분자유선학적 유연관계를 RAPD PCR 방법을 이용하여 구명하였다. 88개의 arbitrary primer중 재현성과 다형성을 기준으로 선발하고, 유연관계분석을 위하여 22개의 primer에서 181개의 RAPD marker를 이용하였다. 유연관계를 위한 조사는 5개 수종, 14개 클론 몇 천연집단의 개체목에 대하여 181개의 다형성 RAPD marker를 가지고, UPGMA와 Neighbor-joining 방법으로 유연관계도를 구했다. 방법을 달리하여 그런 유연관계도에서 각각의 분지에서의 차이는 현 사시 클론간에 미미한 위치변화만 있을 뿐 전체적인 계통수에는 변화가 없었다. 유연관계도에서 수원사시나무는 은백양과 같은 분지군을 형성하였고, 주성분분석에서는 사시나무와 같은 계열을 이루고 있어서 수원사시나무는 이 들 두 종의 1대 교잡종으로 추정되며, 은사시나무는 자연교잡종과 인공교배종이 동일한 유전적 배경을 갖는 것으로 나다났다.

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유전자 분석 기반 수입산 형태 변이 반하 유통 사례 보고 (A Case Report of Imports Morphological Variation of Pinelliae Tuber Based on the Genetic Analysis)

  • 김욱진;최고야;노수민;문병철
    • 대한본초학회지
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    • 제37권5호
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    • pp.9-16
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    • 2022
  • Objectives : The purpose of this study is to report that applying the genetic discrimination method to Pinelliae Tuber is suitable as a countermeasure for the limitations of morphological identification announced publicly in the Ministry of Food and Drug Safety(MFDS). Methods : Randomly selected fifty samples in Pinelliae Tuber imported from China were used for morphological and genetic identification. The morphological identification was applied method announced publicly by the MFDS. The traits of morphological identification were classified as Pinellia ternata, P. tripartita, Pinellia pedatisecta, and Typhonium flagelliforme, according to the formation of tuberous root and tuber morphology. The genetic identifications were conducted by Sequence Characterized Amplified Region(SCAR) marker and DNA barcoding analysis for cross-validation, respectively. SCAR marker was verified according to the presence or absence of amplicon through PCR amplification using species-specific primers. DNA barcoding analysis used sequence information of the matK region. Results : As a result of the morphological identification, 27 out of 50 samples were identified as original species 'P. ternata' of genuine 'Pinelliae Tuber', and 23 were identified as adulterant species 'P. pedatisecta'. Unlike this, the genetic identification was identified as the original species 'P. ternata' in all 50 samples in the SCAR marker and matK regional sequence analysis. Conclusions : Pinelliae Tuber of morphological mutant that can not be classified by morphological identification is imported from China. The SCAR marker would be used as accurate and efficient assays for species identification of the morphological mutant.

수도의 흰등멸구(Sogatella furcifera Horvath)에 대한 저항성 유전자 연관분석 (Linkage Analysis of the Resistance Genes to Whitebacked Planthopper (Sogatella furcifera Horvath) in Rice)

  • 이영태;허문회
    • 한국작물학회지
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    • 제29권2호
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    • pp.136-151
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    • 1984
  • 수도에 있어서 흰등멸구에 대한 저항성 유전자를 가진 것으로 알려진 N22(Wbphl)와 ARC 10239(Wbph2)의 흰등멸구 저항성 유전자의 연관 분석을 하기 위하여 V번 연관군을 제외한 11개 연관군의 표식 유전자를 가진 semi-dwarf 초형의 검정친과 N22및 ARC10239와의 교잡 F$_2$세대에서 12개 표식 유전자의 유전양식을 조사하고 F$_3$ 세대에서 흰등멸구에 대한 저항성의 분리를 조사하여, 이들을 이용하여 연관분석을 한 결과를 요약하면 다음과 같다. 1. N22와 검정친들과의 교잡 세대 F$_2$에서 lg, d-t, g, la, nl, bl, gl 둥은 우성과 열성이 3 : 1로, Bh는 9 : 7로 이론치에 맞게 분리하였으며, Cl, gh, Lh, bc 등 4개 형질은 오차가 컸지만 대체로 이론분리비에 비슷한 분리비를 보였다. 2. ARC 10239와 검정친들과의 조합 F$_2$에서 Cl, lg, Pn, g, la, bl, gl 등은 우성과 열성이 3 : 1로, Bh는 9 : 7로 분리하였으며, gh, Lh, nl, bc 등은 이론분리에 비하여 오차가 크게 나타났다. 3. N22의 Wbph1 유전자와 ARC 10239의 Wbph 유전자는 단순우성유전자이었으며 저항성 계통, 분리계통 및 감수성 계통의 분리비는 모든 조합에서 1 : 2 : 1 의 이론치에 합당하였다. 4. Wbph1은 II번 연관군의 liguleless (lg)와 36.8%의 조환가로 연관되어 있으며 Black hull(Bl)과도 35.9%의 조환가로 연관된 것으로 나타났으며, Bh는 II번 연관군의 Ph(Phenol staining)와 보족적으로 발현됨 을 고찰하였다. 5. Wbph 2는 검정된 I,II,III,IV,VI,VII,VIII,IX,X,XI,XII번 연관군의 Cl, lg, Pn, g, gh, Lh, la, nl, bl, bc, gl, Bh와 독립적인 것으로 나타났다. 6. 이상에서 흰등멸구 저항성 유전자 Wbph2의 연관관계가 분명하지 못한 것은 사용된 Marker 유전자가 염색체상에서 저항성 유전자와 원거리에 있거나 Marker 형질의 발현특성에서 기인된 것으로 생각된다. 7. Wbph2의 연관관계는 본 실험에서 검토되지 못한 V번 연관군을 포함하여 독립성 검정에서 변이가 큰 것으로 나타난 Ⅶ, Ⅷ, Ⅹ 및 XII 번 연관군과의 조합에 대하여는 재검토되어야 할 것으로 판단된다.

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Microsatellite Marker를 사용한 한우 품종 식별력 및 유전적 특성 분석 (Estimation of Genetic Characteristic and Cumulative Power of Breed Discrimination Using Microsatellite Markers in Hanwoo)

  • 오재돈;이진아;공홍식;박경도;윤두학;전광주;이학교
    • 한국수정란이식학회지
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    • 제23권3호
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    • pp.203-209
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    • 2008
  • To estimate the genetic characteristics and cumulative power of discrimination (CPD) existing among Hanwoo (Korean cattle) and exotic foreign population (Angus, Herford, Charolais, Holstein) we used a total of 414 genomic DNAs from five breeds population (Hanwoo, Angus, Hereford, Charolais, Holstein). Genetic characteristics indices including mean allele number among loci, unbiased heterozygosity ($h_i$) within locus and polymorphic information content (PIC) and unbiased average heterozygosity (H) among loci in four breeds were calculated using the generated allele frequencies by each marker. The mean allele numbers for all loci ranged between 5 and 7 while heterozygosity (H) ranged from 0.75 (HW) to 0.64 (HF) among loci and across breeds heterozygosity (H) was 0.69. The generated unbiased average heterozygosity among loci in each breed was integrated to the global formula of CPD resulting in 99.71 % within the populations. The genetic variation of HW (Hanwoo) showed highest estimates among the analyzed breeds.