• 제목/요약/키워드: genetic correlations

검색결과 392건 처리시간 0.022초

산림훼손지복원을 위한 Soil Bioengineering 기술개발(V) - 관목류의 뿌리인발저항 특성 - (Development of the Soil Bioengineering Techniques for Restoring of Degraded Forest Area (V) - Pull-out Resistance Characteristics of Shrubs' roots -)

  • 차두송;오재헌;지병윤;조구현;이해주
    • Journal of Forest and Environmental Science
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    • 제24권2호
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    • pp.111-118
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    • 2008
  • 산림 훼손지 비탈면 녹화에 주로 사용되는 관목류 7종에 대한 인발저항실험을 실시하였다. 조사수종의 평균인발력은 두릅나무 37.26 kgf, 버드나무 34.56 kgf, 국수나무 26.00kgf의 순으로 측정되었으며, 뿌리의 근원경 및 뿌리체적이 크면 클수록 인발력은 높게 산출되었다. 뿌리 근원경과 뿌리 체적에 대한 인발력은 산딸기나무가 가장 상관관계가 높았으며, 산초나무가 가장 낮았다. 그리고 뿌리의 근원경에 따른 인발력은 대부분 유의적인 차이가 있는 것으로 나타났으며, 뿌리체적에 따른 인발력은 뿌리체적이 20 ml미만에서만 수종간의 인발력 차이가 있는 것으로 나타났다.

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VHL Gene Mutation Analysis of a Chinese Family with Non-Syndromic Pheochromocytomas and Patients with Apparently Sporadic Pheochromocytoma

  • Zhang, Bin;Qian, Jing;Chang, De-Hui;Wang, Yang-Min;Zhou, Da-Hai;Qiao, Gou-Mei
    • Asian Pacific Journal of Cancer Prevention
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    • 제16권5호
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    • pp.1977-1980
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    • 2015
  • Objective: The Von Hippel-Lindau syndrome (VHLD), an inherited neoplastic syndrome predisposing to central nervous system hemangioblastoma (CNS), pheochromocytoma (PCC), renal cell carcinoma(RCC), retinal hemangioma (RA) and renal cysts, is caused by mutations or deletions of the VHL tumor-suppressor gene. To assess VHL genotype-phenotype correlations with function of pVHL a gene mutation analysis of members in a Chinese family with non-syndromic PCCs and individuals with apparently sporadic pheochromocytoma (ASP) was performed. Materials and Methods: DNA samples of 20 members from the Chinese family with non-syndromic PCCs and 41 patients with ASP were analyzed by polymerase chain reaction and direct sequencing, confirmed by Taqman probe. Results: Three novel mutations (H125P, 623(^TTTGTtG) and R120T) were identified in the Chinese family and in 3 among 41 ASP patients. The mutations were all located in exon 2 of VHL gene encoding ${\beta}$-domain of pVHL. The tumor type in H125P carriers and R120T carriers was VHL type 2C. And 623(^TTTGTtG) carriers presented VHL type 2B or type 2C. Conclusions: VHL gene abnormalities were identified in the Chinese family with non-syndromic PCCs and patients with APS, resulting in dysfunction of pVHL. H125P and R120T could be associated with VHL type 2C, while 623(^TTTGTtG) might be linked with VHL type 2B or type 2C. Not only is the genetic analysis helpful for early diagnosis and treatment of patients with VHLD, it is also benefitial for research intoVHLD pathogenesis.

Structure and Diversity of Arsenic-Resistant Bacteria in an Old Tin Mine Area of Thailand

  • Jareonmit, Pechrada;Sajjaphan, Kannika;Sadowsky, Michael J.
    • Journal of Microbiology and Biotechnology
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    • 제20권1호
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    • pp.169-178
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    • 2010
  • The microbial community structure in Thailand soils contaminated with low and high levels of arsenic was determined by denaturing gradient gel electrophoresis. Band pattern analysis indicated that the bacterial community was not significantly different in the two soils. Phylogenetic analysis obtained by excising and sequencing six bands indicated that the soils were dominated by Arthrobacter koreensis and $\beta$-Proteobacteria. Two hundred and sixty-two bacterial isolates were obtained from arsenic-contaminated soils. The majority of the As-resistant isolates were Gramnegative bacteria. MIC studies indicated that all of the tested bacteria had greater resistance to arsenate than arsenite. Some strains were capable of growing in medium containing up to 1,500 mg/l arsenite and arsenate. Correlations analysis of resistance patterns of arsenite resistance indicated that the isolated bacteria could be categorized into 13 groups, with a maximum similarity value of 100%. All strains were also evaluated for resistance to eight antibiotics. The antibiotic resistance patterns divided the strains into 100 unique groups, indicating that the strains were very diverse. Isolates from each antibiotic resistance group were characterized in more detail by using the repetitive extragenic palindromic-PCR (rep-PCR) DNA fingerprinting technique with ERIC primers. The PCR products were analyzed by agarose gel electrophoresis. The genetic relatedness of 100 bacterial fingerprints, determined by using the Pearson product-moment similarity coefficient, showed that the isolates could be divided into four clusters, with similarity values ranging from 5-99%. Although many isolates were genetically diverse, others were clonal in nature. Additionally, the arsenic-resistant isolates were examined for the presence of arsenic resistance (ars) genes by using PCR, and 30% of the isolates were found to carry an arsenate reductase encoded by the arsC gene.

Comparative Study of the Nucleotide Bias Between the Novel H1N1 and H5N1 Subtypes of Influenza A Viruses Using Bioinformatics Techniques

  • Ahn, In-Sung;Son, Hyeon-Seok
    • Journal of Microbiology and Biotechnology
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    • 제20권1호
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    • pp.63-70
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    • 2010
  • Novel influenza A (H1N1) is a newly emerged flu virus that was first detected in April 2009. Unlike the avian influenza (H5N1), this virus has been known to be able to spread from human to human directly. Although it is uncertain how severe this novel H1N1 virus will be in terms of human illness, the illness may be more widespread because most people will not have immunity to it. In this study, we compared the codon usage bias between the novel H1N1 influenza A viruses and other viruses such as H1N1 and H5N1 subtypes to investigate the genomic patterns of novel influenza A (H1N1). Totally, 1,675 nucleotide sequences of the hemagglutinin (HA) and neuraminidase (NA) genes of influenza A virus, including H1N1 and H5N1 subtypes occurring from 2004 to 2009, were used. As a result, we found that the novel H1N1 influenza A viruses showed the most close correlations with the swine-origin H1N1 subtypes than other H1N1 viruses, in the result from not only the analysis of nucleotide compositions, but also the phylogenetic analysis. Although the genetic sequences of novel H1N1 subtypes were not exactly the same as the other H1N1 subtypes, the HA and NA genes of novel H1N1s showed very similar codon usage patterns with other H1N1 subtypes, especially with the swine-origin H1N1 influenza A viruses. Our findings strongly suggested that those novel H1N1 viruses seemed to be originated from the swine-host H1N1 viruses in terms of the codon usage patterns.

돼지 생체에서 부분육 형질의 추정 (Estimation of Carcass Cut Traits in Live Pigs)

  • 도창희
    • Journal of Animal Science and Technology
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    • 제49권2호
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    • pp.203-212
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    • 2007
  • 돼지의 부분육의 중량과 수율의 개량을 위하여 생체에서 이를 측정할 수 있는 수단이 필요하다. 4개의 교잡 계통돈 돼지 432두에 대하여 시기별 생체 측정치와 도축자료를 조사하였다. 생체중 및 도체중 그리고 부분육의 중량은 도축일령에 의해 영향을 거의 모두 받고 있지만 수율에 있어서는 삼겹살과 목심에만 유의성있게 영향하는 것으로 나타났다. 부분육의 형질과 시기별 생체 측정치간의 상관 조사에서 대부분의 체측정치에서 검정 종료시의 측정치가 다른 시기의 측정치보다 상관이 높게 나타났고, 부분육 형질의 예측을 위한 회귀식에서도 검정 개시와 출하시의 체측정치 보다 검정 종료시 체측정치를 이용하였을 때 결정계수가 모든 부분육의 형질에서 높아 가장 정확하게 추정하였다. 부분육 형질을 평균 출하일령으로 보정한 뒤, 검정 종료시 체측정치의 3차 다항회귀 식에 의한 부분육 형질의 예측에서 중량과 수율의 결정계수가 각각 0.59~0.68과 0.33~0.43의 범위를 보여주어 이를 돼지의 부분육 형질의 개량을 위해 활용하면 국내 선호부위가 우수한 한국형 종돈의 생산이 기대된다.

Secondary Fish-Odor Syndrome Can be Acquired by Nitric Oxide-mediated Impairment of Flavin-containing Monooxygenase in Hepatitis B Virus-Infected Patients

  • Yi, Hyeon-Gyu;Lee, Jung-Nam;Ryu, Seung-Duk;Kang, Ju-Hee;Cha, Young-Nam;Park, Chang-Shin
    • The Korean Journal of Physiology and Pharmacology
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    • 제8권4호
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    • pp.213-218
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    • 2004
  • Primary fish-odor syndrome (FOS) is a genetic disorder caused by defective flavin-containing mono-oxygenase 3 gene (FMO3) with deficient N-oxidation of trimethylamine (TMA), causing trimethylaminuria (TMAU). By contrast, secondary FOS can be acquired by decreased FMO activities in patients with chronic liver diseases, but the underlying mechanisms are unknown. In the present study, we examined plasma NOx concentrations and viral DNA contents as well as in vivo FMO activities and their correlations in chronic viral hepatitis (CVH) patients. Plasma concentration of NOx was significantly increased by 2.1 fold $(56.2{\pm}26.5\;vs.\;26.6{\pm}5.4\;{\mu}M,\;p<0.01)$, and it was positively correlated with plasma hepatitis B virus (HBV) DNA contents $(r^2=0.2838,\;p=0.0107)$. Furthermore, the elevated plasma NOx values were inversely and significantly correlated with in vivo FMO activities detected by ranitidine-challenged test $(8.3%\;vs.\;20.0%,\;r^2=0.2109,\;p=\0.0315)$. TMA N-oxidation activities determined in CVH patients without challenge test were also significantly low (73.6% vs. 95.7%, p< 0.05). In conclusion, these results suggested that secondary FOS could be acquired by the endogenously elevated NO in patients with CVH.

모녀간의 골밀도 : 신체 측정치 및 체조성, 골지표, 영양소 섭취량 및 에너지 소비량과의 관계 (Bone Mineral Density of Korean Mother-daughter Pairs : Relations to Anthropometric Measurement, Body Composition, Bone Markers, Nutrient Intakes and Energy Expenditure)

  • 이희자
    • Journal of Nutrition and Health
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    • 제29권9호
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    • pp.991-1002
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    • 1996
  • The objectives of this study were to obtain normative data for 45 mother-daughter pairs on spine, femur(neck, ward's triangle, trochanter) and total body BMD (bone mineral density) measured by dual energy X-ray absorptiometry, anthropometric measurement, body composition, biochemical markers, nutrient intakes and energy expenditure, to determin the interrelations of these factors within each group, to measure familial resemblance for each variable. We observed significantly positive correlations between height, weight, head, hip and calf circumferences, tricep, femur and calf skinfold thickness, total lean body mass(=weight-total fat body mass-bone mineral content), protein and fat intakes, Ca index, serum total protein and albumin of monter-daughter pairs(p<.05-p<.001). Among mothers, age, osteocalcin, higher, weight, Ca and energy intakes were predictors of BMDs. Among daughters, weight and energy intake were predictors of BMDs. The BMD in lumbar spine(r=.48, p<.01), femoral neck(r=.38, p<.05), ward's triangle(r=.36, p<.05) of the mothers were significantly correlated with those of the daughters, after adjustment for mother's age, hight, weight, osteocalcin, Ca and energy intakes and daughter's weight, energy intake. In regression analyses, mother's BMD also were positively associated with daughter's BMD in lumbar spine, femoral neck, ward's triangle. Our findings support that mothers with low BMD tended to have daughters with low BMD. In the age groups studied, as well genetic factors as environmental factors may have an important role in determining BMD. This study suggests that women may successfully enhance their genetically determined BMD through adequate nutrient intakes and weight bearing exercise.

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Role of exon 7 PTEN Gene in Endometrial Carcinoma

  • Kafshdooz, Leila;Kafshdooz, Taiebeh;Tabrizi, Ali Dastranj;Ardabili, Seyyed Mojtaba Mohaddes;Akbarzadeh, Abolfazl;Gharesouran, Jalal;Ghojazadeh, Morteza;Farajnia, Safar
    • Asian Pacific Journal of Cancer Prevention
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    • 제16권11호
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    • pp.4521-4524
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    • 2015
  • Background: Endometrial carcinoma is the most common malignant tumor of the female genital tract and the fourth most common cancer in Iranian women after breast, colorectal and lung cancers. Various genetic alterations appear to be early events in the pathogenesis of endometrial carcinoma and it seems that PTEN is the most commonly mutated gene in the endometrioid subtype. The aim of the present study was to investigate the correlation between mutations in exon 7 of PTEN gene and endometrial carcinoma. Materials and Methods: Seventy-five patients with endometrial carcinoma and 75 females whose underwent hysterectomy for non tumoral indication were selected for evaluation of PTEN mutations in exon 7 by PCR-SSCP and sequencing. Correlations between the frequency and type of mutation and the pathologic findings of the cancer (tumor subtype, stage and grade) were assessed. Results: All of the samples were obtained from Iranian patients. 60 % (45 cases) of the tumors were endometriod and 40% (30 cases) were of serous type. The grade distributions of the 75 cases according to the FIGO staging system were as follows: low grade, 20 cases; high grade 55 cases, low stage, 41 cases; high stage 34 cases. For exon 7 of the PTEN gene, the analysis showed that there were no mutations in our cases. Conclusions: Our findings in the present study suggest that exon 7 of PTEN does not play any significant role in the development of endometrial carcinoma in Iranian cases.

Promoter Methylation Status of DNA Repair Gene (hMLH1) in Gastric Carcinoma Patients of the Kashmir Valley

  • Wani, Majid;Afroze, Dil;Makhdoomi, Muzamil;Hamid, Iqra;Wani, Bilal;Bhat, Gulzar;Wani, Rauf;Wani, Khursheed
    • Asian Pacific Journal of Cancer Prevention
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    • 제13권8호
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    • pp.4177-4181
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    • 2012
  • Cancer is a multi-factorial disease and variation in genetic susceptibility, due to inherited differences in the capacity to repair mismatches in the genome, is an important factor in the development of gastric cancer (GC), for example. Epigenetic changes, including aberrant methylation of 5/CpG islands in the promoter regions of mismatch repair (MMR) genes like hMLH1, have been implicated in the development of various types of GC. In the present study we evaluated the role of hMLH1 promoter hypermethylation in Kashmiri GC patients and controls, and assessed correlations with various dietary and lifestyle factors. The study included 70 GC patients (56 males and 14 females; age ($mean{\pm}S.D$) $50{\pm}11.4$ years). Distinction between methylated and unmethylated was achieved with MS-PCR and DNA band patterns. The Chi-square test was applied to assess the risk due to promoter hypermethylation. We found a strikingly high frequency of promoter hypermethylation in GC cases than in normal samples (72.9% (51/70) in GC cases vs 20% (14/70) in normal samples (p=0.0001).We also observed a statistically significant association between methylated hMLH1 gene promoter and smoking, consumption of sundried vegetables and hot salted tea with the risk of GC. This study revealed that hMLH1 hypermethylation is strongly associated with GC and suggested roles for epigenetic changes in stomach cancer causation in the Kashmir valley.

내륙습지 기능의 우선순위 결정 (Determining the priority order of wetland functions)

  • 이승준;최현아;이우균;이종렬;전성우;김준순
    • 환경영향평가
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    • 제23권3호
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    • pp.197-207
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    • 2014
  • In this study, the functional priority of wetlands was determined through analysis of previous research. To determine relative importance, three processes were performed. First, quantitative values from the case studies were normalized. Second, non-quantitative values were prioritized based on standard criteria. Third, equal weight was applied as long as there was no special consideration regarding a particular value's disproportionate priority in the research. Finally, results were grouped into large, medium, and small classes.In this study, the functions of the medium class were found to be the most significant, in the following order of priority: water supply and ground water recharge; culture and recreation; biodiversity; product; water quality control; flood control; erosion control; moderation of climate change; and provision of biochemical matter. To verify these results, we compared our findings with those of an assessment that used the Rapid Assessment Method (RAM) on the same type of study area. Whereas this comparison indicated some correlations by the culture, water storage, and genetic sustainability functions, it suggested a lack of such relationship by the water purification and flood prevention functions.