• 한국식품저장유통학회지
• /
• 제17권6호
• /
• pp.903-907
• /
• 2010

청미래덩굴 뿌리(Smilacis rhizoma)로부터 칼슘과 결합하는 물질을 분리하고자 열수로 추출한 추출물을 ion exchange, normal-phase HPLC 및 gel filtration chromatogarphy를 이용하여 칼슘 결합 물질을 순차적으로 분리하였다. 그 결과 ion exchange chromatography에서 7개의 major peaks를 얻었으며, 이 중 F6 fraction이 0.083 mM로 칼슘과 가장 높은 결합력을 가졌다. 또한 F6를 $NH_2$ column으로 분획한 결과 F61에서 0.130 mM의 가장 높은 칼슘함량을 나타내었으며, 최종적으로 $Superdex^{TM}$를 이용하여 F611 fraction으로 분리하였다. 따라서 청미래덩굴 뿌리 추출물 중 F611 fraction을 이용하여 biomineral을 제조함으로써 칼슘 보충제나 기능성 성분의 원료로써 식품산업에 활용될 수 있다고 판단된다.

• 한국축산식품학회지
• /
• 제30권5호
• /
• pp.746-754
• /
• 2010

Response surface methodology was adopted to model and optimize the effects of microbial transglutaminase (TG) and calcium alginate (CA) systems of various ratios on the gelation characteristics of porcine myofibrillar protein (MP) at various salt levels. The CA system consisting of sodium alginate (SA), calcium carbonate (CC) and glucono-$\delta$-lactone (GdL) showed no remarkable changes in the salt-soluble fraction, and only minor effects on electrostatic interactions were observed. Increasing CA concentration caused acid-induced hydrophobic interactions in MPs, resulting in increased MP gel strength. The TG system, containing TG and sodium caseinate (SC), induced cold-set MP gelation by formation of covalent bonding. The main advantage of the combined system was a higher cooking yield when the MP gel was heated. These results indicated that 0.7% TG combined with 0.8% CA system can form a viscoelastic MP gel, regardless of salt levels.

• 한국가축번식학회지
• /
• 제19권4호
• /
• pp.251-258
• /
• 1996

These studies were carried out to investigate the effect of gonadotropins (GTH), fetal calf serum (FCS), porcine follicular fluid (pFF) and FCS and pFF fractions obtained by the gel filtration on in vitro maturation of porcine follicular fluid. When the oocytes were cultured in TCM-199, the maturation rate was higher in pFF than in FCS in both with or without GTH and in pFF the maturation rate was higher in with GTH than in without GTH. In case of without GTH, pFF increased maturation rates in TCM-199, but not in Whitten's medium (WM). When the oocytes were cultured in WM supplemented with FCS fractions, the maturation rate(51.6%) of oocytes was significantly (P<0.05) higher in fraction B (about 30∼70 kDa) than in control, FCS and other fractions. When oocytes were cultured in WM supplemented with pFF fractions, fractions B (about 30∼70 kDa) and D (about 1∼10 kDa) were significantly (P<0.05) higher than in control, pFF and other fractions. In conclusiion, the addition of gonadotropins into the maturation media was effective for oocyte maturation. The addition of pFF was more effective than addition of FCS for maturation of porcine oocytes in vitro. And fraction B from FCS and fractions B and D from pFF was effective for oocyte maturation.

• 한국염색가공학회:학술대회논문집
• /
• 한국염색가공학회 2008년도 제38차 학술발표대회
• /
• pp.135-137
• /
• 2008

PVA films with 1,2,3,4-Butanetetracarboxylic acid(BTCA) and sodium hypophosphite monohydrate(SHM) were crosslinked via thermal curing. Different parameters affecting on the crosslinking were investigated including BTCA and SHM concentration, curing temperature and time. The cured films was extracted with boiling water and gel fraction was calculated from weight change of the PVA films. Moisture regain of the gelled films was also measured. While the gel fraction of PVA films increased with increasing curing temperature and time, moisture regain decreased. Water resistance of the crosslinked PVA films improved by the BTCA crosslinking treatment.

• 한국환경과학회지
• /
• 제7권1호
• /
• pp.104-111
• /
• 1998

The Dongbok lake water before and after alum coagulation and activated carbon adsorption were analyzed in terms of organic contents, molecular weight distributuin (MWD), and UV-absorbance. Dissolved organic compounds in the Dongbok lake were fractionated into three molecular size classes by gel permeation chromatography. The fractionation was reasonably successful in isolating compounds with The bulk of the dissolved carbon was present in compounds of molecular weight in the range of 3,000~10,000. Alum coagulation preferentially treated molecules of high molecular weight, which has molecules larger than 10,000. The dissolved organic carbon (DOC) removal after activated carbon adsorption was high in the Fraction B , IR . The $A_{260}$/DOC ratio after alum and activated carbon treatment the Fraction II, III. This results suggest that the organics remaining after each treatment has a trihalomethane formation potential

• Journal of Microbiology and Biotechnology
• /
• 제10권2호
• /
• pp.233-237
• /
• 2000

As part of a continuing investigation to identify free radical scavengers from the fruit bodies of basidiomycetes, we isolated two p-terphenyl compounds, designated as PAl and PA2l, from cethanolic extract of the fruit body of Pasillus panuoides. The methanolic extract was processed by ethyl acetate extraction and silica gel column chromatography to yield two active fraction. PAl was obtained from one of the fractions through Sephadex LH-20 and silica gel column chromatographies and reverse-phase HPLC. The other fraction was purified by Sephadex LH-20 and reverse-phase column chromatographies to produce PA2. The compounds PA1 and PA2 were identified as leucomentin-4 and leucomentin-2, respectively, on the basis of various spectroscopic analyses. These compounds exhibited strong inhibitory activities against lipid peroxidation in rat liver microsomes with IC_{50}$ values of 0.10 and $0.06{\;}\mu\textrm{g}ml$, respectively.

• 약학회지
• /
• 제30권6호
• /
• pp.343-347
• /
• 1986

Korean ginseng was purified to obtain radioprotective protein fractions by buffer extraction, ammonium sulfate fractionation, CM-cellulose column chromatography, heat inactivation and Sephadex G-75 column chromatography. The final three fractions, GI, GII and GIII were subjected to Disc-polyacrylamide gel electrophoresis (PAGE) and SDS-PAGE. The molecular weights(M.W.) of native and denatured proteins were estimated by using regression line equations obtained from the mobilities of standard proteins. As the results, in Disc-PAGE, the GI fraction showed two protein bands with M.W. of above 213, 000 and 55, 000, GII showed one band with M.W. of 44, 000 and GIII, also one band with M.W. of 19, 000. In SDS-PAGE, GI fraction gave four subunit bands with M.W. of above 114, 000, 27, 000, 24, 000 and 19, 000, GII gave two bands with M.W. of 46, 000 and 22, 000, and GIII, one band of 19, 000.

• 한국염색가공학회지
• /
• 제22권3호
• /
• pp.214-219
• /
• 2010

Polyethylene(PE) films were photocrosslinked by continuous UV irradiation. Benzophenone addition as low as 1wt% into the PE film increased the gel fraction up to 96%. The photocrosslinking was attributed to the recombination of PE radicals generated upon UV irradiation, which was enhanced by the hydrogen abstraction of the added benzophenone. Also the crossliked PE showed higher thermal stability and decreased crystallinity with increasing UV energy as shown by TGA, XRD and DSC analysis. It was also possible to fix 5.4% octadecane into PE by the photocrosslinking. The crosslinked PE film containing octadecane showed lower tensile strength and modulus coupled with higher extension compared to that without octadecane, which can be used as a new plasticizing method for the crosslinked PE film.

• 한국식품위생안전성학회지
• /
• 제10권4호
• /
• pp.225.1-230
• /
• 1995

The ethanol extract from the root bark of Morus alba showed the strongest antimicrobial activity on the growth of almost all the tested microorganisms which were food-borne pathogens and food-related microorganisms. 1) In order to isolate and purify of antimicrobial substance extracted from the root bark of Morus alba, the antimicrobial substance from the ethanol extract which exhibited a strong antimicrobial activity was purified by solvent fractionation, silica gel column chromatography, TLC and HPLC. Among the fractions fractionated by 4 kind of solvents from the ethanol extract, the antimicrobial activity of ethyl acetate fraction had the strongest antimicrobial activity against B. subtilis. Unknown compound were isolated from the ethyl acetate fraction by silica gel column chromatography, TLC and HPLC and the compounds showed strong absorbance at 207, 217 and 285 nm, therefore, it was supposed to be a kinds of aromatic compound.

• 고려인삼학회:학술대회논문집
• /
• 고려인삼학회 1980년도 학술대회지
• /
• pp.53-57
• /
• 1980

Red ginseng powder was administered at a dose of 2.7 g per day for 3 months to 21 diabetic patients who were under the treatment with insulin. It was found that the ginseng powder was effective to 12 patients and ineffective to 9 patients. Based on these clinical results, experiments were carried out to elucidate factors which concerned with improvement of pathological conditions of diabetes mellitus. In the previous symposium, we reported that red ginseng powder contained an anti-lipolytic peptide, or an insulin-like peptide. In the course of purification of the insulin-like peptide in the ginseng, we found another fraction which possessed anti-lipolytic activity. The anti-lipolytic factor of the fraction was purified by gel filtration on Bio Gel P-2 column and Dowex $50W{\times}4$ column chromatography. The character of the finally purified material was examined by thin-layer chromatography, high-speed liquid chromatography and mass spectrometry. With these examinations, the active principle was indentified to be adenosine. Pharmacological significance of these insulin-like substances, the peptide and adenosine, was discussed.