• 한국수정란이식학회지
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• 제12권2호
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• pp.219-226
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• 1997

Follicular fluid influxed into the oviduct during ovulation may affect movement of sperm for fertilization Thus, in this study, the effect of follicular fluid, obtained from follicles of l0mm in diameter, on number and quality of sperm recovered by swim-up separation was investigated and sperm-movement stimulating components extracted from follicular fluid with methanol and isooctane were separated by gel filtration with Sepadex G-1O, G-25 and G-1OO gels, and were isolated by electrophoresis with SDS-PAGE mini gel. The results obtained were as follows; 1. Diluted follicular fluid stimulated sperm movement. 2. Sperm-movement stimulating factors were in methanol extract. 3. Sperm-movement stimulating effect of methanol extract appeared in fraction I among fractions recovered after gel filtration. And the fraction I contained proteins indicating 4 major bands as about 47, 43, 25 and 14 kilodaldons and 5 minor bands as about 67, 58, 23, 22 and 21 kilodaldons. 4. The fraction I recovered from G-100 gel showed significantly low percentage of motile sperm and had no protein indicating the band of 67 kilodaldons among the minor bands.

• Journal of Pharmaceutical Investigation
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• 제30권3호
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• pp.151-158
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• 2000

Gel and cream containing 5% and 10% ketonic fraction (KF) of Leptospermum scoparium, respectively were formulated. Antimicrobial activity, stability, anti-inflammatory effect, rheological properties, drug release and acute toxicity for these topical efficacy were evaluated. Gel and cream containing neomycin or gentamycin in combination with KF has potent antimicrobial activity. Gel and cream were physically stable and did not show any creaming for 6 months storage. Gel showed plastic flow with yield value and cream showed pseudoplastic flow with hysteresis loop. The gel and cream containing KF showed higher viscosity than control or commercial one. The viscosity increased as the concentration of KF increased. Both 10% gel and cream showed a significant decrease in swelling when applied to the carrageenan- injected paw, suggesting local antiinflammatory activity. Particularly, 10% gel preparation showed similar antiinflammatory activity when compared with commercially available drugs. Percent of drug released and diffusion coefficient were in the order of 5% gel, 10% gel, 5% cream, and 10% cream, respectively. There were no significant changes of body weight in rats percutaneously administered with 10% cream and gel when compared with control. There were no induced acute toxicity when 10% cream or gel was applied to rats. Leptospermum scoparium could be practicaly used in topical preparations.

• 한국염색가공학회:학술대회논문집
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• 한국염색가공학회 2008년도 제39차 학술발표회
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• pp.11-12
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• 2008

Poly(ethylene oxide)(PEO) of molecular weight of 300,000 was crosslinked by UV irradiation in the presence of crosslinker. The photochemical crosslinking of PEO was enhanced by the addition of dimethacrylate crosslinkers in the film. Percent conversion of the polymer into gel as well as water absorbency were investigated gravimetrically. Gel fraction of PEO films increased with increasing crosslinker concentration. In the case of photocrosslinked PEO films with benzophenone, gel fraction reached about 95%. The thermal behavior of crosslinked PEO films was characterized by thermogravimetric analysis. The maximum decomposition temperature increased with increasing crosslinker concentration.

• Archives of Pharmacal Research
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• 제21권3호
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• pp.260-265
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• 1998

Angiogenic activity of Aloe vera gel was investigated by in vitro assay. We obtained the most active fraction from dichloromethane extract of Aloe vera gel by partitioning between hexane and 90% aqueous methanol. The most active fraction (F3) increased the proliferation of calf pulmonary artery endothelial (CPAE) cells. In addition, F3 fraction induced CPAE cells to invade type I collagen gel and form capillary-like tube through in vitro angiogenesis assay, and increased the invasion of CPAE cells into matrigel through in vitro invasion assay. Furthermore, the effect on the MRNA expression of proteolytic enzymes which are key participants in the regulation of extracellular matrix degradation was investigated by northern blot analysis. F3 fraction enhanced mRNA expression of urokinase-type plasminogen activator (u-PA), matrix metalloproteinase-2 (MMP-2), and membrane-type MMP (MT-MMP) in CPAE cells whereas the expression of plasminogen activator inhibitory (PAl-1) mRNA was not changed.

• 한국염색가공학회지
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• 제21권4호
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• pp.39-45
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• 2009

PVA films were crosslinked with dimethylol dihydroxy ethylene urea (DMDHEU) and three polycarboxylic acids of butanetetracarboxylic acid (BTCA), citric acid and malic acid Different factors influencing the crosslinking treatment with BTCA were investigated including BTCA and sodium hypophosphite (SHP) concentration, curing temperature and time. The cured films was extracted with boiling water and gel fraction was calculated based on weight change of the PVA films. The gel fraction of PVA films increased with increasing curing temperature and time. And the resistance to water and thermal stability of the crosslinked PVA films improved with the BTCA crosslinking treatment. While crosslinking with citric acid gave the highest gel fraction among the crosslinkers, crosslinking with malic acid showed the highest absorbancy in 0.9% saline solution, which was attributed to lower crosslink density and high number-average molecular weight between crosslinks. The superabsorbent PVA films could be prepared by adjusting the crosslinking condition of PVA with polycarboxilic acids.

• Plant Resources
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• 제8권2호
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• pp.145-154
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• 2005

The antimicrobial effect of each fraction after fractionation of an ethanol extract of leaf mustard was examined in terms of nucleic acid, chloroform, ethylacetate, and butanol. The ethylacetate fraction, which showed the strongest level of antimicrobial effect among the different ethanol extract fractions of leaf mustard, was isolated and purified using silica gel column chromatography and HPLC, respectively, to obtain a single antimicrobial substance called KLM-1. The antimicrobial effect of this substance was 10 times higher than that of the ethylacetate fraction. A further study is on the way to confirm the structure of the antimicrobial substance KLM-1 through LC/Mass and NMR.

• Journal of Plant Biology
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• 제33권1호
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• pp.73-80
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• 1990

Subcellular distribution of arginase activity was measured in leaves of Canavalia lineata. Both mitochondrial and cytosolic fraction were found to contain the arginase activity. It was noticible that cytosolic fraction contained a substantial amount of arginase activity. Different mobility of arginase from these two fractions was showed on DEAE-Sephacel chromatography and polyacrylamide gel electrophoresis. Also different pI value was showed 6.3 in cytosolic and 6.7, 7.1 in mitochondiral fraction on IEF gel electrophoresis. However, canavaine-dependent-activity (CDA) of arginase in these two fractions were not different. These results indicate that heterogenity of arginase occurs in leaves of C. lineata.

• 한국가축번식학회지
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• 제12권3호
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• pp.141-147
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• 1988

These experiments were carried out to develop new techniques for in vitro separation of x-and Y-bearing spermatozoa. The results obtained in these experiments were summarized as follows: 1. Following centrifugation of discontinuous percoll density gradient, populatin of spermatozoa increased progressively from low to high density. The highest concentration of spermatozoa was observed at the 4th fraction which included 36.6% of spermatozoa. 2. As increasing percoll concentration, the higher motility index was obtained and the highest motility index(74.2) was obtained at the 5th fraction. 3. The percentage of B-body bearing spermatozoa following percoll density gradient centrifugation was decreased from 39.7% to 25.6%. 4. The sperm population following chromatography by sephadex gel and percoll density gradient centrifugation was decreased in 1st, 5th and 6th fractions but the reverse was turn for 2nd, 3rd and 7th fractions, and the highest sperm concentration was observed at the 7th fraction which included 37.4% of spermatozoa. 5. Motility index of spermatozoa was increased from 77.6 to 79.4 after the sephadex gel filtration, however it was decreased at all fractions after percoll density gradient centrifugation. The lowest motility index(33.2) was obtained from the 7th fraction. 6. The rate of B-body bearing spermatozoa was shown the trend to decrease by the sephadex gel filtration and the trend was accelerated by the percoll density gradient centrifugation. The lowest percentage of B-body bearing spermatozoa, 12.0% was obtained from the 5th fraction.

• 방사선산업학회지
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• 제3권1호
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• pp.31-37
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• 2009

This paper describes the preparation of manufacturing a new nanocomposite material, which involves adding a multi walled carbon nanotube (MWCNT) to improve thermal characteristics of poly (ethylene-co-vinyl acetate) (EVA). We irradiated the prepared nanocomposites with doses of 50 kGy, 100 kGy and 200 kGy at a dose rate of $5kGy\;hr^{-1}$ and examined their thermal stability, activation energy and crosslink level by using a thermogravimetric analyzer (TGA) and gel fraction experiments. TGA results indicated that the samples with a MWCNT had higher Derivative Thermo Gravimetry (DTG) 2nd peak temperatures than those without a MWCNT. And activation energy of the samples reduced as the absorption dose and the MWCNT content increased. Finally, the gel fraction increased rapidly up to 100 kGy as total absorption dose increased, and then the growth rate of all samples was slowly increased from 100 kGy.

• 한국식품과학회지
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• 제12권4호
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• pp.254-262
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• 1980

저자(著者) 등(等)은 Arthorbacter luteus로부터 분리(分離)된 $zymolyase(endo-{\beta}-1,\;3-glucanase)$에 관(關)한 일련의 연구과정(硏究過程)에서 조(粗) zymolyase의 표품중(標品中)에 zymolyase의 $Saccharomyces\;sak{\acute{e}}$ 세포벽(細胞壁)에 대한 용해(溶解) 활성(活性)을 촉진(促進)시키는 인자(因子)의 존재(存在)를 발견(發見)하였다. 따라서 조(粗)zymolyase중(中)의 그 촉진(促進) 인자(因子)를 분리(分離)함과 동시(同時)에 촉진(促進) 인자(因子)에 관(關)한 연구(硏究)를 진행(進行)함에 있어서 불가흠(不可欠)한 zymolyase의 정제(精製)도 아울러 시도(試圖)하였다. 그 결과(結果)는 다음과 같다. 1. 조(粗) zymolyase는 Biogel CM-30 이온 교환(交換) 크로마토그래피에 의해서 column을 그대로 통과(通過)한 세 개의 피크를 포함(包含)한 다섯 개의 피크$(A{\sim}E)$로 분획(分劃)되었다. 2. 다섯 개의 피크 중에서 pH 7. 5, 0. 025M 인산(燐酸)완충액으로 용출(溶出)된 피크 E(protease fraction)만이 zymolyase fraction의 용해(溶解) 활성(活性)을 약(約) 10배(倍)까지 상승(上昇)시키는 용해(溶解) 촉진(促進) 효과(酵果)가 인정(認定)되었다. 따라서 검색(檢索)코져하는 용해(溶解) 촉진(促進) 인자(因子)는 protease fraction 중에 존재(存在)함이 확인(確認)되었다. 3. Sephadex G-75 gel를 사용(使用)한 affinity adsorption chromatograph에 의해서 zymolyase fraction(peak D)으로부터 비용해성(非溶解性) ${\beta}-1$, 3-glucanase, protease 및 불활성(不活性) 단백질(蛋白質)이 배제(排除)된 L-c fraction이 얻어졌다. Polyacrylamide gel disc 전기(電氣) 영동(泳動)에서 L-c fraction은 pH 4. 3 gel에서는 명확(明確)한 단일(單一)의 단백질(蛋白質) 밴드를 보여주었으나, pH 8.3 gel에서는 아직도 두 개의 이상(以上)의 밴드가 검출(檢出)되었다. 4. 그러나 L-c fraction은 효모(酵母) 세포벽(細胞壁)의 다당류(多糖類) 성분중(成分中)에서 오직 glucan에 대한 ${\beta}-1$, 3-glucanase의 기질(基質) 특이성(特異性)만을 나타내었다. 따라서 L-c fraction을 zymolyase의 부분(部分) 정제(精製) 표품(標品)으로써 용해(溶解) 촉진(促進) 인자(因子)에 관(關)한 연구(硏究)에 제공(提供)하고져 동결(凍結) 건조(乾燥)하였다.