• 대한본초학회지
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• 제30권1호
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• pp.19-24
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• 2015

Objectives : This study was carried out to know the necessity of freezing and boiling process of Gastrodiae Rhizoma. Also we need to evaluate Gastrodia elata floral axis as a product ingridients. Methods : Frozen Gastrodiae Rhizoma (GF1, GF2) and Gastrodia elata floral axis (GFA) were prepared. They were divided into samples (GF1 : frozen at the freezer, GF2; frozen and boiled for 10 hours, GFA; dried at 7 $0^{\circ}C$ for 120 hours) for experiment. They were extracted using water, freeze dried and powdered. And we analyzed proximate compositions, free sugars, gastrodin, p-hydroxybenzyl alcohol and p-hydroxybenzyl aldehyde content, phenolic and flavonoid, electron donating ability and nitrate scavenging activity and antioxidant activity. Results : In moisture, crude ash, fructose, glucose, sucrose, p-Hydroxybenzyl alcohol GF2 showed lower level than GF1. But GF2 showed higher content than GF1, in crude fat (0.8% > 0.19%), gastrodin ($8.84{\pm}0.58%$ > $4.18{\pm}0.73%$), and p-hydroxybenzyl aldehyde ($2.45{\pm}0.26%$ > $2.07{\pm}0.16%$) content, phenolic ($9.98{\pm}0.07%$ > $3.35{\pm}0.03%$) and flavonoid ($3.01{\pm}0.06%$ > $1.09{\pm}0.04%$) content, electro donating ability ($15.21{\pm}6.51%$ > $10.44{\pm}4.78%$), nitrate scavening activity ($20.43{\pm}5.30%$ > $13.62{\pm}5.78%$). GFA has a relatively lower key indicators component, but has a enough impact on antioxidant effect in phenolic ($11.85{\pm}0.08%$) and flavonoid content ($1.45{\pm}0.03%$), electron donating ability ($18.58{\pm}9.06%$) and nitrate scavenging activity ($19.41{\pm}9.90%$). Conclusions : In the view of proximate compositions, free sugars, functional component and antioxidant activity, the results indicated that boiling process is effective for the frozen Gastrodiae Rhizoma. And Gastrodia elata floral axis has a significantly functional components and antioxidant activity.

• 한국버섯학회지
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• 제16권2호
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• pp.90-95
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• 2018

본 연구는 실내시설재배에서 천마의 생육단계를 균사활착기, 괴경형성기, 괴경비대기, 휴면기로 구분하고, 최적 온도 및 배양기간을 설정하였으며, 괴경비대기에 변온처리를 하였다. 천마의 균사활착기는 $20^{\circ}C$에서 30일, 괴경형성기는 $25^{\circ}C$에서 120일, 괴경비대기는 $6{\sim}24^{\circ}C$에서 60일, 휴면기는 $5^{\circ}C$에서 30일로 처리하여 총 배양기간은 240일로 설정하였다. 실내시설재배는 노지재배보다 균사활착기는 30일 단축되었고, 괴경형성기는 30일 연장되었으며, 휴면기는 120일 단축됨으로써, 전체 재배기간이 약 120일 단축되었다. 따라서, 본 연구 결과, 밀폐된 실내시설재배에서 온도 조건을 제어하면 천마를 연중생산 할 수 있는 생육모델의 기초자료로 활용될 수 있을 것으로 판단되었다.

• 한국균학회지
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• 제32권1호
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• pp.39-44
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• 2004

천마의 퇴화 현상을 해결하고 우수한 품질의 종마를 얻을 수 있는 천마 종자의 최적 발아 조건을 조사하였다. 천마종자는 상수리나무 낙옆 배지에서 발아가 가장 잘되었으며, 또한 자연수분보다는 인공수분에서 얻은 종자가 결실률이 높고 안정적이었다. 천마 종자 발아는 생중량 41 mg 이상의 중숙꼬투리나 완숙꼬투리에서 양호하였으며, 파종은 종자 수확 직 후가 가장 좋았으며, 장기 보존 시에는 $5^{\circ}C$ 이하에서 가능하였다. 천마 생육 시 뽕나무버섯균이 천마의 영양원으로 이용되는 것처럼 천마 종자 발아 시에도 균류가 영양원으로 이용되는 것으로 추정된다.

• 한국자원식물학회지
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• 제31권4호
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• pp.372-377
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• 2018

본 연구는 천마의 종자생산을 위한 수확시기별 처리 방법에 따른 자마 생산 효과를 구명하고자 수행하였다. 봄수확 천마는 괴경의 무게를 60-99 g, 100-139 g, 140-179 g, 180 g 이상으로 처리하였고, 가을수확 천마는 $5{\pm}1^{\circ}C$의 저온을 2, 4, 6, 8, 10주 처리하였다. 그 결과, 봄수확 천마는 괴경의 무게가 100 g 이상일 경우, 인공수정률이 90.9%-94.8%, 원괴체 형성률이 3.1%-5.4%, 자마 형성률이 10.1%-15.3%로 나타났다. 가을수확 천마는 4주 이상의 저온처리 시 인공수정률이 70.4%-87.6%, 원괴체 형성률이 2.2%-2.6%, 자마 형성률이 8.7%-9.5%로 나타났다. 따라서, 종자 및 자마 생산을 위해서는 봄수확 천마는 100 g 이상의 괴경을 사용해야 하고, 가을수확 천마는 저온에서 4주 이상의 휴면타파가 필요했다. 가을수확 천마를 이용할 경우 봄수확 천마에 비해 인공수정률, 원괴체 및 자마 형성률이 낮았지만, 자연 발아 상태보다 월등히 높았다. 따라서 일정한 괴경의 무게와 저온처리 휴면타파를 이용하면 천마의 종자와 자마가 확보됨으로써 천마의 연중생산이 가능할 것으로 판단되었다.

• 대한약학회:학술대회논문집
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• 대한약학회 2002년도 Proceedings of the Convention of the Pharmaceutical Society of Korea Vol.2
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• pp.390.1-390.1
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• 2002

Gastrodia elata Blume belongs to Orchidaceae, its steamed and dried rhizomes are very important herbal medicines used for the medical treatment of headaches. migraine. dizziness, epilepsy, rheumatism. neuralgia. paralysis and other neuralgic and nervous disorders. Although phytochemical studies of the rhizome have revealed the presence of several phenolic compounds. in which a phenolic glycoside. gastrodin (C13H18O7). is a major consituent, so far, there is no report on the studies of its aerial part. (omitted)

• 대한약학회:학술대회논문집
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• 대한약학회 2003년도 Proceedings of the Convention of the Pharmaceutical Society of Korea Vol.2-2
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• pp.206.3-207
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• 2003

The bioassay-guided fractionation of the methylene chloride soluble portion of a methanol extract of Gastrodia elata tubers led to the isolation of a new furfural, 5-(4-hydroxy-benzyloxymethyl)-furan-2-carbaldehyde (2), together with four known compounds (1, 3-5), which exhibited potent inhibitory activity at the concentration of 25 $\mu\textrm{g}$/ml on melanin biosynthesis in cultured B-16 mouse melanoma cells.

• 대한약학회:학술대회논문집
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• 대한약학회 2002년도 Proceedings of the Convention of the Pharmaceutical Society of Korea Vol.2
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• pp.299.2-299.2
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• 2002

For the activity-guided separation on anti-asthmatic action from 4 fractions as n-hexane (yield. 0.09%), EtOAc (0.48%), BuOH (3.0%) and H2O (5.17%) fractions from MeOH extract (11.64%) of powdered Gastrodia elata Rhizoma (GER), some biological active agents were isolated by column chromatography (column, silica gel: elution solvent. CHCl3 : MeOH) according to the method of Junko Hayashi et. al. and Heihachiro Taguchi et. al. (omitted)

• 대한약학회:학술대회논문집
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• 대한약학회 2002년도 Proceedings of the Convention of the Pharmaceutical Society of Korea Vol.2
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• pp.300.1-300.1
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• 2002

From 4 fractions as n-hexane (yield. 0.09%), EtOAc (0.48%). BuOH (3, 0%) and H2O (5.17%) fraction from MeOH extract (11, 64%) of powdered Gastrodia elata Rhizoma (GER) for the activity-guided separation on anti-inflammatory action. some biological active agents were isolated by column chromatography (column. silica gel: elution solvent. CHCl3 : MeOH) according to the method of Junko Hayashi et. al. and Heihachiro Taguchi et. al. Compound I, II, III, IV, V as phenolic derivatives were isolated in the EtOAc and BuOH fractions. (omitted)

• Journal of Pharmaceutical Investigation
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• 제29권1호
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• pp.47-53
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• 1999

Gastrodia elata (GE) is an oriental medicinal herb which has been used traditionally for the treatment of various brain diseases including convulsion and epilepsy. The purpose of this study was to determine pharmacokinetic parameters of 4-hydroxy-3-methoxybenzaldehyde (HMBA) and p-hydroxybenzaldehyde (PHBA), constituents of GE, in rats. Male rats were cannulated in the femoral vein, femoral artery, bile duct and ureter. They received a single i.v. bolus dose of either HMBA or PHBA through the femoral vein. The concentration of HMBA or PHBA in plasma, bile and urine samples were analyzed by reversed-phase HPLC. HMBA and PHBA have very short half-lives, i.e. 4.03 and 2.26 minutes respectively. Most of HMBA and PHBA were thought to be eliminated through metabolism as the metabolized fraction approaches unity. Derivatives of HMBA or PHBA with longer biological half-lives should be designed to develop better anticonvulsants and more complete qualitative and quantitative understanding of the overall pharmacokinetic fate of these compounds awaits further investigation.

• 생약학회지
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• 제30권3호
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• pp.284-289
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• 1999

In order to find active ingradients having an agonistic activity to benzodiazepine receptor from Gastrodia elata Blume (Orchidaceae) which has been used as an anticonvulsant in oriental medicine, one component and some fractions were separated from the butanol extract of the rhizomes of this plant and evaluated for their activities on GABA/benzodiazepine receptor in vitro. As a result, one crude mixture (F4f) obtained from the most active fraction (F4) inhibited significantly the binding of $[^3H]Ro15-1788$, a selective benzodiazepine receptor antagonist, to benzodiazepine receptor of rat cortices. GABA significantly enhanced the inhibition of $[^3H]flunitrazepam$ binding by F4f, and this positive GABA shift supported the strong possibility of the agonistic activity of F4f to benzodiazepine receptor.