• Korean Journal Plant Pathology
• /
• v.14 no.2
• /
• pp.136-144
• /
• 1998

Many cloves of native cultivated garlics in Korea were found to be infested by mites when observed with stereo-microscope. The mite was identified by light and scanning electron microscopic observation as Aceria tulipae. Surveying viruses from the vegetatively propagated garlic, highly flexuous, filamentous particles (700∼800 nm) were detected in Aceria tulipae, local lesions of Chenopodium murale after sap transmissions, mosaic garlic leaves inoculated with mite-borne virus by transmission of Aceria tulipae and naturally infected garlic leaves. The mite-borne virus isolated did not react with antisera of aphid-borne potyviruses (LYSV-G, LYSV-L, WoYSV) or carlavirus (GLV), but reacted with antisera of garlic mite-borne viruses (GV-C, GMbMV). In ultratin sections of mite-borne virus infected garlic tissues, aggregates of virus particles and membrane proliferations were found in the parenchyma cells, but cytoplasmic cylindrical inclusions were not observed. Heavily mite-infested plants showed streaking and malformation due to mite feeding. The mite-borne virus was identified as garlic mite-borne mosaic virus (GMbMV), the mite-borne genus Rymovirus of the Potyviridae by mite transmission, morphology of virus particles, serological relationships, host range, distribution pattern of virus particles and inclusion bodies in the infected cells. The results demonstrate that mite-borne virus is one of the major viruses infecting native cultivated garlic plants showing mosaic or streak symptoms in Korea.

• Journal of Microbiology and Biotechnology
• /
• v.15 no.5
• /
• pp.1110-1114
• /
• 2005

Six viruses of the genera Carlavirus (Garlic mosaic virus, GarMV, and Garlic latent virus, GarLV), Allexivirus (Garlic virus X, GarV-X, and Garlic mite-borne filamentous virus, GarMbFV) and Potyvirus (Leek yellow stripe virus, LYSV, and Onion yellow dwarf virus, OYDV) from Korean garlic plants with mosaic symptoms were simultaneously detected by multiplex RT-PCR and subsequently sequenced. An immunocapture RT-PCR for the detection of GarLV, LYSV, and OYDV was also performed. The coat protein phylogenetic analysis of the garlic viruses showed that the Korean isolates were most closely related to the isolates from China, Japan, Brazil, and Argentina. This study is the first report for the differentiation of six garlic viruses in Korea by simultaneous detection using multiplex RT-PCR.

• Proceedings of the Korean Society of Plant Pathology Conference
• /
• 1999.04a
• /
• pp.51.3-51
• /
• 1999