• 한국임상수의학회지
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• 제31권3호
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• pp.212-215
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• 2014

이 연구는 사육황새에 급여하는 먹이(양미리와 전갱이)의 차이에 따른 혈청 단백질 분획의 변화를 평가하기 위한 것이다. 사육황새를 먹이에 따라 두 그룹(그룹 1과 2)으로 나누어 연구를 진행하였다. 그룹 1에는 22마리, 그룹 2에는 29마리의 황새가 포함되었고, 각각 양미리와 전갱이를 급여하였다. 두 그룹에서 전혈구검사, 혈청화학검사, 단백질분획검사(${\alpha}$-, ${\beta}$- 및 ${\gamma}$-글로불린) 및 지단백질 분획검사(HDL 및 LDL)를 진행하고 결과를 비교하였다. 검사 결과 그룹 1에서 ${\alpha}$-글로불린의 감소와 ${\beta}$-글로불린의 증가가 유의적으로 관찰되었다. 그룹 1에서 LDL의 농도는 그룹 2에 비해 유의적으로 증가하였다. 결과적으로 양미리를 급여한 사육황새에서 ${\beta}$-글로불린의 증가가 LDL의 증가에 의한 것임을 확인하였다.

• 한국가축번식학회지
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• 제12권3호
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• pp.162-166
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• 1988

Holstein cows being raised in the Kangweon district were examined on the values of blood chemistry in infertile cows. The results obtained were as follows; 1. Total serum protein was 7.52$\pm$0.45g/100ml 2. Serum albumin was 3.06$\pm$0.28g/100ml 3. In values of serum globulin, $\alpha$1-globulin, $\alpha$2- globulin, $\beta$-globulin and ${\gamma}$-globulin were 0.79$\pm$0.14, 0.60$\pm$0.10, 0.86$\pm$0.13 and 2.21$\pm$0.35ng/100ml, respectively. 4. A/G ration was 0.69$\pm$0.09 5. Total cholesterol in serum was 119.73$\pm$34.52mg/100ml 6. Blood glucose was 55.24$\pm$6.46mg/100ml 7. Serum calcium was 9.27$\pm$0.87mg/100ml 8. Serum inorganic phosphorus was 5.74$\pm$0.86mg/100ml 9. Serum glutamic oxalacetic transaminase was 47.90$\pm$11.02I$\mu$/l 10. Serum glutamic pyruvic transaminase was 14.20$\pm$3.02I$\mu$/l

• Applied Biological Chemistry
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• 제22권1호
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• pp.1-9
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• 1979

대두(大豆)의 ${\gamma}-conglycinin$을 정제(精製)하고 그의 물리일(物理一) 화학적(化學的) 성질(性質)을 연구(硏究)하여 다음의 결과(結果)를 얻었다. Gamma-conglycinin은 대두(大豆)의 pH 4.5, 0.2M Nacl 추출액을 Sephadex G-150으로 분리하여 얻은 7S globulin을 DEAE-Sephadex A-50으로 column chromatography하여 정제(精製)하였다. 정제된 ${\gamma}-conglycinin$은 면역전기영동(免疫電氣泳動), polyacrylamide gel 전기영동(電氣泳動)과 gel isoelectric focusing 상으로 순수(純粹)하였다. 등전점(等電點)은 pH 5.4이었으며 질소(窒素) 16.21%, mannose 4.18, glucosamine 1.21%의 함량(含量)이었다. 아미노산 조성(組成)은 일반적(一般的)으로 lysine, dicarboxylic acid와 암모니아태(態) 질소(窒素)의 함량(含量)이 높았고 함황(含黃)아미노산과 tryptophane의 함량(含量)은 낮은 편이었다. Subunit의 등전점(等電點)은 pH $4.5{\sim}5.5$에 분희(分希)하였는데 그중 pH $4.6{\sim}5.0$에 위치(位置)한 것은 분자량(分子量) 38,000의 당(糖) peptide이었으며 pH $5.0{\sim}5.5$의 것은 분자량(分子量) 32,000의 단순(單純) peptide이었다.

• Restorative Dentistry and Endodontics
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• 제2권1호
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• pp.20-26
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• 1976

Cellulose acetate electrophoresis was used for differential diagnosis of radicular cysts and granulomas. Sixteen periapical lesions were excised from sixteen teeth with radiographic evidence of periapical pathosis, and two normal periodontal tissues were surgically removed from two wisdom teeth. The tissue samples were separated into two parts. Half was prepared for cellulose acetate electrophoresis, and the electrophoretic patterns were scanned with Helena densitometer. The other half was examined histologically, and the histologic diagnoses were then compared with the electrophoretic patterns and with the x-ray findings. The results were the following: 1. The histopathologic results showed the presence of 13 granulomas (81.8%) and 3 cysts (18.3%). 2. An albumin pattern, alpha-globulin pattern, beta-globulin pattern, fibrinogen pattern and gamma-globulin pattern were found in all eighteen cases. 3. All eighteen cases were divided into normal periodontal tissue group, dental granuloma group, and radicular cyst group by the histopathologic diagnosis. The electrophoretic pattern of dental granuloma group and that of normal periodontal tissue group resembled each other. The two groups showed the highest percentage in beta-globulin fraction, and the beta-globulin of dental granuloma group(47.17%) was higher than that of normal periodontal tissue group (40.54%) by 6.63%. On the contrary, beta-globulin fraction of radicular cyst group (28.00%) was much lower than those of the granuloma group (47.17%) and normal periodontal tissue group (40.54%), and alpha-globulin of radicular cyst group (34.16%) was much higher than those of the granuloma group (20.04%) and normal group (19.58%). 4. Five cases (31.3%) of the sixteen periodontal lesions showed different results between histopathologic diagnoses and radiographic findings.

• 대한약리학회지
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• 제12권2호
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• pp.21-32
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• 1976

The influence of Panax ginseng and Acanthopanax on the metabolism of hens are studied experimentally. The hens are fed with three dose levels of ethanol extract of Panax ginseng and Acanthopanax for 24 months and for comparing, there are control hens and young control hens. The results are as follows. 1. Hemoglobin, hematocrit and erythrocyte sedimentation rate values of hens fed with Panax ginseng and Acanthopanax are not significantly. different from control group. There is tendency of decreasing of erythrocyte sedimentation rate with aging. 2. The serum total lipid values of hens fed with Panax ginseng and Acanthopanax have tendency of increment compared with control group, especially in large dose treated hens of Panax ginseng and it is statistically significant. The serum cholesterol levels of all treated hens seem to be increased without significance statistically. Serum triglyceride level is higher in young hens compared with aged, and Panax ginseng and Acanthopanax treated hens reveal much higher values than control hens. So the increment of triglyceride levels in the Panax ginseng and Acanthopanax treated hens may be mainly contribute to increment of total lipid content. 3. Long-term administration of Panax ginseng and Acanthopanax seem to increase total serum protein content. Such increment seem to be due to the increment of globulin, especially gamma globulin, which is statistically significant in the large dose group of Acanthopanax. There are no differences of serum albumin levels of all the groups. 4. The blood glucose level and the glycogen content of liver and muscle have no difference among all the experimental groups. To summarize the results, the effects of Panax ginseng and Acanthopanax on the metabolism of hens have similarity in the majority of data of this experiment, and seem to be mainly influence to lipid and protein metabolisms by increasing serum triglyceride and globulin, especially gamma globulin.

• Journal of Preventive Medicine and Public Health
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• 제24권1호
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• pp.1-7
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• 1991

The concentrations of serum proteins fractions and their electrophoretical patterns were investigated in 135 patients with coal workers' pneumoconiosis who participated in confirmative examination for pneumoconiosis in December 1989. Their radiographical profusions were classified as 1/0 or more. Agarose film and phosphoric acid-sodium hydroxide buffer(pH 9.6) were used for electrophoresis. Concentration of each protein fractions and electrophoretical patterns seemd to be equivalent to reference values. Serum ${\alpha}_1$- and $\beta$-globulin concentrations, however, were significantly different (p<0.50) among categories of small opacity profusions and showed the lowest level in the group of category 1. Albumin concentratins decreased and ${\alpha}_2$- globulin concentrations increased significantly (p<0.05) in the group of complicated with pulmonary tuberculosis. $\gamma$-globulin concentrations were not varied by category of profusions nor by pulmonary tuberculosis complication.

• 한국임상수의학회지
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• 제33권5호
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• pp.255-260
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• 2016

$Pyunkang-tang^{(R)}$ is a kind of herbal medicine and has been used for the treatment of atopy and allergic disease in humans over forty years. The purpose of this study was to evaluate the hematological, biochemical, and protein and lipid electrophoresis profiles after the oral administration of $Pyunkang-tang^{(R)}$ in healthy dogs. Fifteen clinically healthy beagle dogs were selected and orally administered either 33 ml of $Pyunkang-tang^{(R)}$ (group I, n = 5), 16.5 ml of $Pyunkang-tang^{(R)}$ (group II, n = 5), or 33 ml of distilled water (group III, n = 5) 3 times a day for 4 weeks. The results of the hematological, serum biochemical, and urine analysis did not differ significantly among the 3 groups. The oral administration of $Pyunkang-tang^{(R)}$ for 4 weeks was associated with significant changes in the serum globulin levels and an elevation in the high-density lipoprotein (HDL) concentrations in groups I and II (p < 0.05), compared to group III. The ${\alpha}1-globulin$ and ${\gamma}-globulin$ levels were significantly increased in group I, and the ${\alpha}1-globulin$ and ${\beta}-globulin$ levels were significantly increased in group II. The ${\alpha}2-globulin$ levels were significantly decreased in both groups. During the short-term evaluation of $Pyunkang-tang^{(R)}$ administration, we did not detect any specific adverse effects in the dogs. However, further evaluation of the safety and efficacy of $Pyunkang-tang^{(R)}$ for the treatment of atopic disease in dogs is needed.

• 대한수의학회지
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• 제37권2호
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• pp.451-456
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• 1997

120 heads of korean native calves were examined of serum protein by using cellulose acetate electrophoresis. During 20 days since the calves were purchased, serum protein, fibrinogen values and plasma : fibrinogen ratio were examined in the calves with respiratory and diarrheal disease. The result obtained in this investigation were as follows : 1. Among the 120 heads of calves that were purchased from market, 14 heads(22%) of calves were occurred respiratory disease, and 12 heads(20%) of calves are occurred diarrhea. Occurrence of respiratory disease was 14.5(4~20) days in average and diarrhea was 9.6(2-15) days after they had been purchased. 2. Serum protein fraction were analyzed by cellulose acetate electrophoresis. ${\beta}-globulin$, A/G ratio and ${\beta}_2-globulin$ values were decreased in the calves with respiratory disease. Especially, ${\beta}_2-globulin$ were significantly decreased. In calves with diarrhea, there was no change in ${\beta}-globulin$ values. ${\beta}_2-globulin$ values were higher than that of the normal and respiratory diseased calves. 3. ${\alpha}-globulin$ values were increased in both of calves with diarrhea and respiratory disease. This tendency was due to increase ${\alpha}_2-globulin$ values. 4. The $\gamma$-globulin value of calves with diarrhea was the lowest among the 3 groups. 5. The total protein values of normal calves were $7.0{\pm}1.1g/dl$ and that of respiratory and diarrheal diseased calves were $6.9{\pm}0.9g/dl$ and $6.6{\pm}0.8g/dl$, respectively. Total protein value of calves with diarrhea was lower than that of normal and respiratory diseased calves. Globulin value of calves with diarrhea was the lowest among them. The low value of total protein in diarrheal diseased calves was due to decrease globulin values. 6. The fibrinogen values of calves with respiratory disease ($643{\pm}189mg/dl$) were significantly higher than that of normal calves($533{\pm}135mg/dl$) and calves with diarrhea($572{\pm}188mg/dl$). The plasma : fib. ratio of respiratory diseased calves was $12.0{\pm}4.9$, normal calves was $13.8{\pm}3.5$ and diarrheal diseased calves was $12.8{\pm}4.6$. The ratio of the calves with respiratory disease was significantly decreased.

• 한국가축번식학회지
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• 제23권2호
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• pp.119-125
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• 1999

본 연구는 오골계에 있어서 성장에 따른 gamma -globulin, lipoprotein 및 중량변동과, Fabricius 낭과 흉선의 적출시 발생 5일령부터 150일령까지의 혈청 protein 농도와 Ig, 백혈구수 및 림프구수의 변동을 조사하고자 수행하였다. 1. 재래계와 오골계의 성장에 따른 gamma-globulin 농도는 발생 당일로부터 5일령까지는 각각 0.41$\pm$0.01~0.85$\pm$0.05 mg/㎗ 와 0.50$\pm$0.01~0.98$\pm$0.08 mg/㎗ 로 증가하였으나 10일령부터 감소하여 낮은 치를 나타내다가 재래계는 100일령부터 오골계는 발생 50일령 이후부터 증가된 수준을 나타냈다. 2. 오골계의 성장에 따른 혈청 alpha-lipoprotein, beta-liporptein 및 gamma -lipoprotein 농도는 각각 74.1$\pm$6.8~240.2$\pm$9.7 mg/㎗, 89.7$\pm$5.7~225.8$\pm$11.3 mg/㎗ 및 87.6$\pm$4.7~220.3$\pm$10.2 mg/㎗ 수준이었다. 오골계의 성장에 따른 혈청 alpha-lipoprotein 농도는 점차적으로 큰 감소를 나타냈다. 3. 오골계의 Fabricius 낭과 흉선 적출후 성장에 따른 백혈구 및 림프구수는 10일령부터 100일령까지 증가경향을 나타냈으며, 흉선 적출군의 백혈구 및 림프구수는 대조군에 비해 낮게 나타났다. 4. 오골계의 Fabricius 낭과 흉선 적출후 성장에 따른 혈청 Ig 농도는 Fabricius 낭 적출군의 경우 대조군에 비해 큰 차이가 없었으나 50일령 이후에는 증가하기 시작하여 100~150일령에서는 5일령에 비해 높은 치를 나타냈으며, 흉선적출군은 성장에 따른 유의한 증가경향을 나타냈다.

• 미생물학회지
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• 제21권1호
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• pp.15-26
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• 1983

Commercial deerhorns were extracted in boiling water, the deerhorn extracts were per os introduced into rabbits, and then the effects of the extracts on the antibody productions aginst Escherichia coli antigens were investigated for 4 weeks. The experimental rabbits were divided into 4 groups ; control, only deerhorn, only antigen, and antigen plus deerhorn treated groups. The effects of the treatments were measured by counting the number of blood cells, weighing, and immunoelectrophoresis. The rabbits' body weights gained up to 185% in the deerhorn group, and the other groups gained nearly 120%. The numbers of red blood cells in the antigen plus deerhorn group increased somewhat. However, the numbers of leucocytes gradually increased after one week in the antigen group, and at 4 weeks increased up to 290%. In the antigen plus deerhorn group the numbers of leucocytes increased suddenly up to 189% at one week, but after one week the numbers recovered to normal state. Strangely in the deerhorn group the numbers decreased up to 40%. The amounts of serum globulin increased in the control after one week, but maintained about 130%. In the deerhorn group the amounts increased like the control, but after 4 weeks increased up to 175%. In the antigen group the amounts were not changed until 2 weeks, but after 3 weeks abruptly increased over 175%. In the deerhorn plus antigen group the amounts increased gradually up to 262% until 3 weeks, after 3 and 4 weeks the amounts did not increase. The amounts of serum .gamma.-globulin decreased in the control group, and in the deerhorn group and antigen group the amounts did not change until 2 weeks, but after 3 weeks abruptly increased over 175%. In the deerhorn plus antigen group the amounts increased gradually up to 262% until 3 weeks, after 3 and 4 weeks the amounts did not increase. The amounts of serum ${\gamma}-globulin$ decreased in the control group, and in the deerhorn group and antigen group the amounts did not change until 3 weeks, but after 4 week the amounts slightly increased up to 110%. However, the amounts in the deerhorn plus antigen group did not change until 2 weeks, but after 2 weeks abruptly increased up to 174%. The recognized immunoglobulins were IgG and IgM, and the enhanced immunoglobulin was IgG.