• Title/Summary/Keyword: gamma radiation

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Control of Postharvest Bacterial Soft Rot by Gamma Irradiation and its Potential Modes of Action

  • Jeong, Rae-Dong;Chu, Eun-Hee;Park, Duck Hwan;Park, Hae-Jun
    • The Plant Pathology Journal
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    • v.32 no.2
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    • pp.157-161
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    • 2016
  • Gamma irradiation was evaluated for its in vitro and in vivo antibacterial activity against a postharvest bacterial pathogen, Erwinia carotovora subsp. carotovora (Ecc). Gamma irradiation in a bacteria cell suspension resulted in a dramatic reduction of the viable counts as well as an increase in the amounts of DNA and protein released from the cells. Gamma irradiation showed complete inactivation of Ecc, especially at a dose of 0.6 kGy. In addition, scanning electron microscopy of irradiated cells revealed severe damage on the surface of most bacterial cells. Along with the morphological changes of cells by gamma irradiation, it also affected the membrane integrity in a dose-dependent manner. The mechanisms by which the gamma irradiation decreased the bacterial soft rot can be directly associated with the disruption of the cell membrane of the bacterial pathogen, along with DNA fragmentation, results in dose-dependent cell inactivation. These findings suggest that gamma irradiation has potential as an antibacterial approach to reduce the severity of the soft rot of paprika.

Effect of Electron Beam and ${\gamma}$-Ray Irradiation on the Curing of Epoxy Resin

  • Kang, Phil-Hyun;Park, Jong-Seok;Nho, Young-Chang
    • Macromolecular Research
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    • v.10 no.6
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    • pp.332-338
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    • 2002
  • The effect of an electron beam and ${\gamma}$-ray irradiation on the curing of epoxy resins was investigated. Diglycidyl ether of bisphenol A (DGEBA) and diglycidyl ether of bisphenol F (DGEBF) as epoxy resin were used. The epoxy resins containing 1.0-3.() wt% of triarylsulphonium hexafluoroantimonate(TASHFA) and triarylsulphonium hexafluorophosphate(TASHFP) as initiator were irradiated under nitrogen at room temperature with different dosage of EB and ${\gamma}$-rays from a Co$^{60}$ u source. The chemical and mechanical characteristics of irradiated epoxy resins were compared after curing of EB and ${\gamma}$-ray irradiation. The thermal properties of cured epoxy were investigated using dynamic mechanical thermal analysis. The chemical structures of cured epoxy were characterized using near infrared spectroscopy. Mechanical properties such as flexural strength, modulus were measured. The gel fraction of DGEBA with ${\gamma}$-ray was higher than that of the epoxy with EB at the same dose. Young's modulus of the sample irradiated by ${\gamma}$-ray is higher than that of sample cured by EB. From the result of strain at yield, it was found that the epoxy cured by ${\gamma}$-ray had a higher stiff property compared with the irradiated by EB.

Effects of Low Dose Gamma Radiation and Seed Moisture Content on Germination and Early Growth of Vegetable Crops (저선량 방사선 조사 시 종자수분함량이 채소류의 발아와 초기생장에 미치는 영향)

  • Baek, Myung-Hwa;Lee, Young-Keun;Lee, Young-Bok;Yang, Seung-Gyun;Kim, Jae-Sung
    • Korean Journal of Environmental Agriculture
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    • v.22 no.3
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    • pp.215-219
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    • 2003
  • To investigate the effects of low dose gamma radiation and seed moisture content (SMC) on germination and early growth of vegetable crops, seeds of chinese cabbage (Brassica campestris L.), radish (Raphanus sativus L.), red pepper (Capcicum annuum L.), figleaf gourd (Cucurbita ficifolia Bouche) and bottle gourd (Lagenaria siceraria Standl), with different SMC were irradiated with different doses ($0{\sim}20\;Gy$) of gamma-ray by irradiator ($^{60}Co$, ca.150 TBq of capacity, AECL). Vegetable crops in which low dose gamma radiation was irradiated in seeds with different moisture content showed different response in seed germination and early growth to low dose gamma radiation. The germination rate of chinese cabbage, figleaf ground and bottle gourd irradiated with $2{\sim}8\;Gy$ showed interactive responses against relative SMC. Also, significant interactions occurred for the early growth between those factors. The stimulating effects of gamma radiation were more pronounced for hydrated seeds of chinese cabbage, radish, figleaf gourd and bottle gourd showing prominent responses with $2{\sim}10\;Gy$ irradiation, particularly for chinese cabbage and bottle gourd. These results suggest that radiation may promote germination and early growth of vegetable crops through interaction with SMC.

Analysis of Radiation Characteristics of Microstrip Patch Antennas Integrated with Mushroom-like EBG Structures (Mushroom 형태의 EBG 구조를 집적한 마이크로스트립 패치안테나의 방사 특성 해석)

  • Kwak, Eun-Hyuk;Kim, Tae-Young;Kim, Boo-Gyoun
    • Journal of the Institute of Electronics Engineers of Korea TC
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    • v.46 no.8
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    • pp.67-78
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    • 2009
  • Radiation characteristics of microstrip patch antennas integrated with mushroom-like EBG structures in all directions and length direction on a substrate with the relative dielectric constant of 10 are systematically analyzed. As the substrate thickness increases, the effect of the surface wave on the input impedance and radiation pattern of a patch antenna increases. The effect of EBG structures on the input impedance of a patch antenna is negligible when the distances between EBG structures and the center of a patch antenna are 0.4 ${\gamma}_0$, 0.2 ${\gamma}_0$ and 0.1 ${\gamma}_0$ for the substrate thickness of 3.2 mm, 1.6 mm and 0.8 mm, respectively. The forward radiation is improved due to the suppression of surface wave when the periods of EBG structures integrated are larger than 2, 2, 3 periods for the substrate thickness of 3.2 mm, 1.6 mm and 0.8 mm, respectively. The effects of EBG structures on the radiation characteristics of a patch antenna integrated with EBG structures in all directions and length direction are similar.

Absolute $^{56}Mn$ Activity Measurement by $4{\pi}{\beta}-{\gamma}$ Conincidence Counting Technique ($4{\pi}{\beta}-{\gamma}$ 동시계수기술에 의한 $^{56}Mn$방사능 절대측정)

  • Hwang, Sun-Tae;Choi, Kil-Oung;Oh, Pil-Jae;Lee, Kyung-Ju;Lee, Kun-Jai
    • Journal of Radiation Protection and Research
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    • v.12 no.2
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    • pp.19-27
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    • 1987
  • In order to determine the $^{56}Mn\;{\gamma}$-detection efficiency of a $MnSO_4$ bath system, it is essential to do the absolute activity measurement of $^{56}Mn$ solution. For the fabrication of $^{56}Mn$ samples, a 13.718 mg of $^{56}Mn$ metal flake with 99.99% purity was irradiated for 12 minutes at the thermal neutron field of about $10^{13}n/cm^2s$ of flux density. The neutron activated $^{56}Mn$ metal sample was dissolved in 50 ml of 0.1 N-HCl solution. The $^{56}Mn$ samples were fabricated by using the dissolved stock solution and the activity of each of them was measured by the $4{\pi}{\beta}-{\gamma}$ coincidence counting technique. The obtained result was 408.070 kBq/mg with total uncertainty of 0.366% at reference date, 0 h on October 15, 1987.

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Changes in Allergenicity of Porcine Serum Albumin by Gamma Irradiation

  • Kim, Koth-Bong-Woo-Ri;Lee, So-Young;Song, Eu-Jin;Park, Jin-Gyu;Lee, Ju-Woon;Byun, Myung-Woo;Kim, Kyu-Earn;Ahn, Dong-Hyun
    • Food Science of Animal Resources
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    • v.30 no.3
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    • pp.397-402
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    • 2010
  • Pork is an excellent source of essential nutrients such as protein. However, pork can trigger hypersensitivity and serum albumin of pork is known as major allergen. In this study, to evaluate the effect of gamma irradiation on the allergenicity of porcine serum albumin (PSA), PSA solution was irradiated at 3, 5, 7, 10, 15, and 20 kGy. The changes in the ability of PSA to bind IgG and patient's serum caused by gamma irradiation were observed by ci-ELISA and immunoblotting. SDS-PAGE was used for measuring the conformational change of gamma-irradiated PSA. The ability of 3-kGy-irradiated PSA to bind p-IgG and patient's serum was decreased to 30% and 15%, respectively. The binding ability showed no significant differences among all irradiated samples. SDS-PAGE showed that the irradiated PSA bands were degraded and aggregated. Immunoblotting of irradiated PSA revealed that IgG and patient's serum were rarely recognized at 3 kGy. Therefore, gamma irradiation could be applied to less-allergenic pork products.

Radiation Measurement of a Operational CANDU Reactor Fuel Handling Machine using Semiconductor Sensors (ICCAS 2003)

  • Lee, Nam-Ho;Kim, Seung-Ho;Kim, Yang-Mo
    • 제어로봇시스템학회:학술대회논문집
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    • 2003.10a
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    • pp.1220-1224
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    • 2003
  • In this paper, we measured the radiation dose of a fuel handling machine of the CANDU type Wolsong nuclear reactor directly during operation, in spite of the high radiation level. In this paper we will describe the sensor development, measurement techniques, and results of our study. For this study, we used specially developed semiconductor sensors and matching dosimetry techniques for the mixed radiation field. MOSFET dosimeters with a thin oxide, that are tuned to a high dose, were used to measure the ionizing radiation dose. Silicon diode dosimeters with an optimum area to thickness ratio were used for the radiation damage measurements. The sensors are able to distinguish neutrons from gamma/X-rays. To measure the radiation dose, electronic sensor modules were installed on two locations of the fuel handling machine. The measurements were performed throughout one reactor maintenance cycle. The resultant annual cumulative dose of gamma/X-rays on the two spots of the fuel handling machine were 18.47 Mrad and 76.50 Mrad, and those of the neutrons were 17.51 krad and 60.67 krad. The measured radiation level is high enough to degrade certain cable insulation materials that may result in electrical insulation failure.

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Observation Systems of Cherenkov Radiation from Water Phantom Irradiated with Co-60 Gamma-rays

  • Tabushi, Katsuyoshi;Koyama, Shuji;Homma, Mitsuhiko;Tamiya, Tadashi;Yajima, Mihoko;Imai, Kuniharu;Obata, Yasunori
    • Proceedings of the Korean Society of Medical Physics Conference
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    • 2002.09a
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    • pp.123-125
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    • 2002
  • Blue light of Cherenkov radiation generated by electrons in transparent substances such as water and acrylic resin is well known generally. If students can easily observe the blue light at school, they may be impressed by the fascinating radiation. Four years ago, management of the Co-60 unit for radiotherapy was transferred to Nagoya University School of Health Sciences from a related hospital. We have examined whether or not the Cherenkov radiation in water from secondary electrons generated by Co-60 gamma-rays can be safely observed by eyes and photographs. First, the Cherenkov radiation in the water tank was led to the corridor outside the irradiation room by a mirror, and observed directly without any effect of the radiation exposure. Second, photographs of the Cherenkov radiation were taken under the conditions consisted of several irradiation fields and pass lengths of gamma-rays in water.

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Study on the Mechanism of Radiation-induced MCP-1 Expression in RAW264.7 Macrophage Cells (RAW264.7 대식세포에서 방사선에 의한 MCP-1 발현 기작 연구)

  • Jin, Chang Hyun;Park, Yong Dae;Choi, Dae Seong;Jeong, Il Yun
    • Journal of Radiation Industry
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    • v.4 no.3
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    • pp.227-231
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    • 2010
  • The purpose of this study was to investigate the expression mechanism of MCP-1 in gamma-irradiated RAW 264.7 macrophage cells. MCP-1 plays an important role in attracting monocyte to injured site at the early inflammation stage. However the production mechanism of MCP-1 by gamma-irradiation in RAW 264.7 macrophage cells was almost undiscovered. We found that MCP-1 was produced in RAW 264.7 macrophage cells by irradiation with 5 Gy. And these inceases were attenuated by specific inhibitors treatment, such as $NF-{\kappa}B$, JNK, ERK, JAK2, and Pyk2. These results indicate that radiation-induced MCP-1 production is mediated by MyD88- and TRIF-dependent pathways in RAW 264.7 macrophage cells. Furthermore, gamma-irradiation induced heme oxygenase-1 (HO-1) expression in RAW 264.7 macrophage cells. However this induction level was reduced before MCP-1 and $IFN-{\beta}$ production.

Effect of Concentration of Carboxymethycellulose on Degradation by Radiation (Carboxymethycellulose의 농도에 따른 방사선 분해 연구)

  • Kim, Jeongsoo;Sung, Nak-Yun;Kim, Jae-Hun;Kim, Tae-woon;Lee, Ju-Woon;Choi, Jong-il
    • Journal of Radiation Industry
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    • v.4 no.4
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    • pp.385-389
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    • 2010
  • In this study, the effect of the concentration of carboxymethylcellulose (CMC) solution on the degradation by irradiation was investigated. The CMC solutions with different concentrations of 3%, 4%, 5%, 6% and 7% were irradiated at the doses of 5, 10, 15, 20, 25 and 30 kGy with gamma ray or electron beam, and the viscosity of CMC solution was measured. The viscosity of the CMC solutions was decreased with an increase in the irradiation dose, but the extent of the degradation by an irradiation was found to be decreased with an increase of the CMC concentration in the solution. The dependency of the irradiation sources showed that an electron beam radiation had degraded the CMC less severely than gamma ray.