• 한국식품저장유통학회지
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• 제6권1호
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• pp.48-54
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• 1999

The effect of irradiation of heat treatment either alone or in combination on the shelf-life of mixed mountain edible herb drinks (MMEHD) was investigated during storage. The MMEHD made from fresh Spuriopinella bracycarpar, Ligularia fischeri and Aster scaber was gamma -irradiated at doses of 0.1 kGy to 1 kGy. Microbial population, color change, vitamin C content, and sensory quality were evaluated during storage at 4, 25 and 35oC. Heated MMEHD induced the growth of total counts, mold and yeast as compared to the non-heated MMEHD. While some reduction in the microbial growth was observed in 1kGy-irradiated groups of both with or without heating. In both groups, L and b values decreased, but a value increased during storage. Also, heating drinks showed increased L and b values and reduced a value, compared to the non-heating drinks. Non-heated drinks showed 82% loss of vitamin C, whereas 25% loss of vitamin C was occurred in the irradiated drinks during storage at 4oC for 4days. In the meanwhile, non-irradiated heating drinks showed 99% loss of vitamin C, but irradiated heating drinks showed 58-65% reduction of vitamin C. Non-irradiated drinks without heating showed more bright color than irradiated ones, but irradiated drinks showed more enhanced brightness during storage. Also, irradiated drinks showed better falvor, sensory quality, and overall acceptability than non-irradiated drinks.

• Asian-Australasian Journal of Animal Sciences
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• 제32권10호
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• pp.1521-1527
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• 2019

Objective: To study the contribution of plant enzyme and microbial activities on protein degradation in silage, this study evaluated the nitrogen transformation dynamics during ensiling of non- and irradiated alfalfa (Medicago sativa L.) and red clover (Trifolium pratense L.). Methods: Alfalfa and red clover silages were prepared and equally divided into two groups. One group was exposed to ${\gamma}$-irradiation at a recommended dosage (25 Gky). Therefore, four types of silages were produced: i) non-irradiated alfalfa silage; ii) irradiated alfalfa silage; iii) non-irradiated red clover silage; and iv) irradiated red clover silage. These silages were opened for fermentation quality and nitrogen components analyses after 1, 4, 8, and 30 days, respectively. Results: The ${\gamma}$-irradiation successfully suppressed microbial activity, indicated by high pH and no apparent increases in fermentation end products in irradiated silages. All nitrogen components, except for peptide-N, increased throughout the ensiling process. Proteolysis less occurred in red clover silages compared with alfalfa silages, indicated by smaller (p<0.05) increment in peptide-N and free amino acid N (FAA-N) during early stage of ensiling. The ${\gamma}$-irradiation treatment increased (p<0.05) peptide-N and FAA-N in alfalfa silage at day 1, whereas not in red clover silage; these two nitrogen components were higher (p<0.05) between day 4 and day 30 in non-irradiated silages than the irradiated silages. The ammonia nitrogen and non-protein nitrogen were highest in non-irradiated alfalfa silage and lowest in irradiated red clover silage after ensiling. Conclusion: The result of this study indicate that red clover and alfalfa are two forages varying in their nitrogen transformation patterns, especially during early stages of ensiling. Microbial activity plays a certain role in the proteolysis and seems little affected by the presence of polyphenol oxidase in red clover compared with alfalfaa.

• 식물병연구
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• 제21권3호
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• pp.193-200
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• 2015

Gray mold caused by Botrytis cinerea is one of the most important postharvest fungal pathogens of cut flowers. Here, gamma irradiation, an alternative for phytosanitary purposes, and sodium dichloroisocyanurate (NaDCC) were used to control B. cinerea in a cut chrysanthemum (Chrysanthemum morifolium Ramat.) cultivar, 'Baekma', one of the cultivars susceptible to B. cinerea. Spore germination and mycelium growth of B. cinerea were inhibited by gamma irradiation in an inversely dose-dependent manner. A dose of 4 kGy completely inhibited the mycelium growth of B. cinerea. A significant change in flower quality (physical properties) on chrysanthemum was shown from gamma irradiation at over 0.2 kGy (p<0.05). Therefore, in this study, the integration of gamma ray (below 0.2 kGy) and NaDCC, an eco-friendly form of chlorine, was investigated to control the disease with low dose of gamma irradiation dose. Interestingly, the gamma irradiated flowers showed more disease severity than the non-irradiated flowers. The combined treatment of gamma irradiation and NaDCC does not affect the severity of the fungal disease, whereas only 70 ppm of NaDCC treatment showed a significantly reduced severity. These results suggest that only chlorination treatment can be applied to control B. cinerea in cut chrysanthemum flowers.

• Journal of Plant Biotechnology
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• 제4권4호
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• pp.163-170
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• 2002

Radiation induced and somaclonal variations were investigated in the regenerates from gamma irradiated and controlled embryogenic callus (EC) of sweetpotato cvs., Yulmi and White Star by morphological, RAPD and AFLP analysis. Most (approx. 90%) of the EC produced somatic embryos developed into plantlets after being transferred to the auxin-free medium. The frequency of morphological variants derived from the irradiated callus ranged from 3 to 7.8% compared to 0.1-1.1% of that derived from the non-irradiated. Morphological variants were selected from the regenerates and analyzed by RAPD and AFLP procedures. RAPD polymorphisms of Yulmi and White Star regenerates from irradiated calli were 8.8% and 6.1%, respectively. However, the polymerphisms among regenerates from the non-irradiation treatment in these two cultivars were non-detectable and 3%, respectively. AFLP polymorphisms of Yulmi and White Star regenerates from irradiated calli were 29.9% and 28.6%, respectively. while the frequencies for those form non-irradiated calli were 8.5% and 5.6%, respectively. Both the control plants and variants from the nonirradiated were clustered together, while variants from irradiated were separated from the group by Nearest-Neighbor-Interchange Branch Swapping Abbreviation: EC (Embryogenic callus), AFLP (Amplified Fragment Length Polymorphism), RAPD (Random amplified polymorphic DNA)

• 방사선산업학회지
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• 제8권1호
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• pp.1-5
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• 2014

This study investigated the reduction of microbial population and sensory properties in red pepper powders irradiated by gamma ray, electron beam, and X-ray. Populations of total aerobic bacteria and yeast & molds in red pepper powders were decreased by irradiation treatment in a dose-dependent manner. Gamma ray, electron beam, and X-ray at doses above 8 kGy caused 100% inhibition on growth of aerobic bacteria in red pepper powders. Inhibitory activity of X-ray on sterilization of red pepper powders was significantly equal to or higher compared to gamma ray and electron beam. Color and off flavor in red pepper powders were no significant difference among the control and samples irradiated with gamma ray, electron beam, and X-ray. As a result, the gamma ray, electron beam, and X-ray irradiation can be used to sterilize the microbial growth in red pepper powders without quality loss.

• 한국환경농학회지
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• 제21권3호
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• pp.202-207
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• 2002

참박과 호박 채종종자의 발아율을 증진시킬 수 있는 종자처리 방법을 확립하고자 저선량 감마선을 1$\sim$20 Gy 까지 조사하여 발아와 초기생육 및 생리활성을 관찰하였다. 저선량 조사한 참박과 호박종자의 발아율은 대조구보다 증가하는 경향을 보였는데 그 효과는 종자상태에 따라 다르게 나타났다. 참박 70$\sim$80% 발아율종자의 발아는 4$\sim$20 Gy에서 증가하였는데 특히 4 Gy조사에서 가장 높았고, 80$\sim$90% 발아율종자도 4 Gy 조사에서 효과를 보였다. 파종 4일 후의 발아율은 참박 70$\sim$80%와 80$\sim$90% 발아율 종자의 경우는 4 Gy조사에서 대조구보다 각각 66%와 26% 증가하였고, 호박의 70$\sim$80% 발아율종자는 2 Gy 조사에서, 80$\sim$90% 발아율종자는 8 Gy 조사에서 향상되었다. 저선량 조사한 유묘생육은 발아율이 증가한 선량에서 증가하였는데 특히 호박 70$\sim$80% 종자의 2 Gy 조사에서 가장 높았다. 파종 6일 후에 조사한 참박의 효소활성은 4 Gy 조사시에 단백질과 catalase 활성이, 호박의 경우는 catalase와 peroxidase 활성이 2 Gy와 8 Gy 조사에서 증가하였다. 참박과 호박종자의 발아와 유묘생육 및 생리활성은 저선량 감마선 조사에 의해 촉진됨을 확인할 수 있었다.

• 한국식품저장유통학회지
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• 제8권1호
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• pp.9-15
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• 2001

밤(Castanea crenata, 은기)의 저장특성에 대하여 수침처리와 감마선 조사의 영향을 검토하기 위하여, 수확된 밤을 48시간 동안 수침 처리한 후 0, 0.15, 0.25 및 0.35 kGy의 감마선을 조사하고 $0^{\circ}C$와 95$\pm$2%의 상대습도에서 9개월 동안 저장하면서 품질변화를 측정하였다. 발아현상은 수침 처리된 밤에서만 저장말기에 나타났으며, 감마선을 0.35 kGy 조사한 밤에서는 발아되지 않았다. 수침처리 밤에서는 조사 선량에 무관하게 저장 초반기부터 부패가 발생되어 수침처리 되지 않은 경우보다 저장 9개월 후 약 2배의 부패율을 보였다. 중량감소는 수침 처리된 밤이 무처리 밤보다 적었으며, 조사선량의 영향은 없었다. 과육경도는 수침처리를 하지 않은 경우 감마선 조사에 의해 감소가 억제되었으며, 고선량 일수록 높게 유지되는 경향이었다. 전당함량은 감마선이 조사된 밤이 조사되지 않은 밤보다 저장말기에 높게 유지되었으며 선량에 따른 차이는 없었다. 비타민 C 함량은 감마선 조사 직후 다소 감소되었으나 저장 중 변화는 감마선 조사된 밤에서 완만하게 감소되었다. 가용성 탄닌의 함량은 발아억제 선량 범위의 감마선 조사에 영향을 받지 않았다.

• Journal of Ginseng Research
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• 제19권3호
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• pp.231-236
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• 1995

The antioxidant activity and the pharmacologically active constituents such as nucleosides and phenolic substancs of Panax ginseng C.A. Meyer (white ginseng powder) treated with gamma irradiation (Rl group) and ethylene oxide fumigation (EO group) were compared with those of non-treated ginseng (control group). High performance liquid chromatograms of nucleosides in three groups were similar with each other. The bathochromic effect of phenolic substances at W absorption region tends to be higher for EO group than Rl group. This tendency was also observed in heat treatment of each group. However, the antioxidant activity may have some effects on the labile property of phenolic substances. In summary the gamma-irradiated ginseng was more stable than the ethylene oxide-treated ginseng, but both ginseng samples gave the similar antioxidant activity.

• 환경생물
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• 제23권4호
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• pp.398-404
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• 2005

Radioprotection is of practical importance for the normal tissues of tumor patients subject to radiotherapy, people with planned or accidental exposure to radiation, and the public and radiation workers. Since oxygen enhances radiation - induced biological damage, antioxidants should be related with the function as a radioprotectors. Ascorbic acid or caffeine is an essential component and antioxidant in the diet of humans and a small range of other mammals. The present study investigates functional radioprotection of caffeine and ascorbic acid against gamma radiation in irradiated C57BL/6N mice. Eight-week-old male C57BL/6N mice were irradiated with 6.5 Gy. A caffeine treated group was administered with $80mg\;kg^{-1}$ body weight by intraperitoneal injection, a single treatment 1 hr before irradiation. Ascorbic acid was administered $330\;mg\;L^{-1}$ in drinking water through all the experimental period. According to time schedules, animals were sacrificed by cervical dislocation. And the samples were collected 2 weeks after whole- body gamma irradiation. The caffeine treated group showed lower decrement of body and organ weights than the other experimental groups. The qualitative analysis of circulating testosterone in serum was performed by means of radioimmunoassay (RIA). The normal level of circulating testosterone was maintained by the treatment of caffeine and ascorbic acid. The change of weight of body and organ and the appearance of seminiferous tubules were improved by an effect of caffeine or ascorbic acid against irradiation. Taken together, caffeine and ascorbic acid protects impairment of spermatogenesis against gamma radiation and may act as a radio-protector.

• 방사선산업학회지
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• 제5권4호
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• pp.383-391
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• 2011

This study was conducted to investigate the applicability of gamma irradiation for improving the quality of paprika and Japanese apricot extract-treated nitrite free pork jerky. Pork jerky was gamma-irradiated at 0, 3, 5, 7 and 10 kGy and physiochemical properties such as proximate composition, 2-thiobarbituric acid (TBARS) values, color stability, texture, and sensory property were then evaluated. The results showed that the treatment of paprika and Japanese apricot extract to the pork jerky increased Hunter color value and texture property and at the same time decreased TBARS values. When gamma irradiated, natural pigment extract-treated pork jerky did not produce any change in its proximate composition (moisture, crude protein, crude lipid contents), and TBARS values. However, the redness (a-value) of pork jerky increased as the irradiation dose increased, whereas shear force of pork jerky was decreased. Sensory result showed that gamma irradiation induced to decrease the sensory scores. Therefore, these results suggest that gamma irradiation and the addition of paprika and Japanese apricot extracts could be an effective mean to improve color and texture of restructured pork jerky without use of nitrite.