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Molecular Characterization and Tissue-specific Expression of a Novel FKBP38 Gene in the Cashmere Goat (Capra hircus)

  • Zheng, X.;Hao, X.Y.;Chen, Y.H.;Zhang, X.;Yang, J.F.;Wang, Z.G.;Liu, D.J.
    • Asian-Australasian Journal of Animal Sciences
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    • v.25 no.6
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    • pp.758-763
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    • 2012
  • As a member of a subclass of immunophilins, it is controversial that FKBP38 acts an upstream regulator of mTOR signaling pathway, which control the process of cell-growth, proliferation and differentiation. In order to explore the relationship between FKBP38 and mTOR in the Cashmere goat (Capra hircus) cells, a full-length cDNA was cloned (GenBank accession number JF714970) and expression pattern was analyzed. The cloned FKBP38 gene is 1,248 bp in length, containing an open reading frame (ORF) from nucleotide 13 to 1,248 which encodes 411 amino acids, and 12 nucleotides in front of the initiation codon. The full cDNA sequence shares 98% identity with cattle, 94% with horse and 90% with human. The putative amino acid sequence shows the higher homology which is 98%, 97% and 94%, correspondingly. The bioinformatics analysis showed that FKBP38 contained a FKBP_C domain, two TPR domains and a TM domain. Psite analysis suggested that the ORF encoding protein contained a leucine-zipper pattern and a Prenyl group binding site (CAAX box). Tissue-specific expression analysis was performed by semi-quantitative RT-PCR and showed that the FKBP38 expression was detected in all the tested tissues and the highest level of mRNA accumulation was detected in testis, suggesting that FKBP38 plays an important role in goat cells.

Design of wideband microstrip monopole slot antenna (광대역 마이크로스트립 모노폴 슬롯안테나의 설계)

  • Lee, Young-Soon;Cho, Yun-Ki
    • Journal of Advanced Navigation Technology
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    • v.16 no.5
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    • pp.766-772
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    • 2012
  • In the present study, wideband microstrip monopole slot antennas which can be applied to the RF4CE Zigbee remote controller are designed. First I-shaped monopole slot antenna which has ${\lambda}g/4$ length at 2.45GHz is designed. In particular, a conducting via is used to connect the microstrip feed line and the ground plane surrounded with the etched slot for the bandwidth improvement. In order to reduce the antenna size, it is changed into L-shaped and T-shaped monopole slot antennas for which improve results of antenna performance are observed. In case of T-shaped monopole slot antenna, impedance bandwidth(VSWR<2) is about 3.32GHz, and also its radiation efficiency and gain is more than 90% and 2.1dBi respectively at whole operating frequency range. In particular, all of proposed monopole slot antennas have the end-fire radiations which has a maximum radiation power toward direction of open ends of monopole slots.

Conflict Resolution of Patterns for Generating Linked Data From Tables (테이블로부터 링크드 데이터 생성을 위한 패턴 충돌 해소)

  • Han, Yong-Jin;Kim, Kweon Yang;Park, Se Young
    • Journal of the Korean Institute of Intelligent Systems
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    • v.24 no.3
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    • pp.285-291
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    • 2014
  • Recently, many researchers have paid attention to the study on generation of new linked data from tables by using linked open data (e.g. RDF, OWL). This paper proposes a new method for such generation of linked data. A pattern-based method intrinsically has a conflict problem among patterns. For instance, several patterns, mapping a single header of a table into different properties of linked data, conflict with each others. Existing studies have sacrificed precision by applying a statistically dominant pattern or have ignored conflicting patterns to increase precision. The proposed method finds appropriate patterns for all headers in a given table by connecting patterns applied to the headers. Experiments using DBPedia and Wikipedia showed results that conflicts of patterns are effectively resolved by the proposed method.

Experimental Results of Ship-To-Ship Lightering Operations Applied Velocity Information GPS

  • Yoo, Yun-Ja;Pedersen, Egil;Kouguchi, Nobuyoshi;Song, Chae-Uk
    • Journal of Navigation and Port Research
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    • v.38 no.6
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    • pp.577-583
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    • 2014
  • A ship-to-ship (STS) lightering operation takes place in order to transfer cargo (e.g. crude oil or petroleum products) between an ocean-going ship and a service ship alongside it. Instrumental measurements to accurately determine the relative speeds and distances during the approach between the vessels would benefit the operational safety and efficiency. A velocity information GPS (VI-GPS) system, which uses the instantaneous velocity measures from carrier-phase Doppler measurement, has been applied in a field observation onboard a service ship (Aframax tanker) approaching a ship-to-be-lightered (VLCC) in open waters. This article proposes to apply VI-GPS as the input sensor to a guidance and decision-support system aiming to provide accurate velocity information to the officer in charge of an STS operation. A method for precise velocity measurement using VI-GPS was described and the measurement results were compared each other with the results of Voyage Data Recorder (VDR) and VI-GPS that showed the concept of a guidance and decision-support system applying VI-GPS with the field test results during STS operations. Also, it turned out that VI-GPS has sufficient accuracy to serve as an input sensor from the field test results.

A New Type of the Measuring and Anti-Caking Bag for Powder Materials (새로운 형태의 분체용 계량적 Anti-Caking 봉지)

  • Kim, Joong-Man
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.15 no.4
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    • pp.51-55
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    • 1986
  • The general bag for powder materials such as sugar, salt and detergent used at present is a simple rectangular bag. In the practical uses, it has a lot of faults: it is difficult to reseal after partial usages, occurrence of loss in the case of sharing or adding, difficult for measured uses. Author made a new type of the measuring bag for powder materials in order to improve the problems. The new type of the bag for powder materials consists of a body, fall-induced tube with length scale line, and a pin to open and reseal. It shows various effectiveness: measured use$({\pm}0.6g)$ of powder materials, eatings don't occur during long period after opening, and no skin contamination during use of detergent. Consequently, the new one Is suitable for the measured use of powder materials such as sugar, salt, detergent, MSG and so on.

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Cloning and Characterization of the IgA Fc Receptor from Swine

  • Chen, Yumei;Liu, Yunchao;Zhang, Gaiping;Feng, Hua;Ji, Pengchao;Wang, Guoqiang;Liu, Chang;Song, Yapeng;Su, Yunfang;Qiao, Songlin;Wang, Aiping
    • Journal of Microbiology and Biotechnology
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    • v.26 no.12
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    • pp.2192-2198
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    • 2016
  • The myeloid-specific IgA Fc receptor ($Fc{\alpha}R$) is a cell surface molecule on immunocytes that provides a fundamental connection between humoral and cellular immunity. In this study, the full-length cDNA sequence of swine $Fc{\alpha}RI$ ($swFc{\alpha}RI$) was isolated and characterized and found to contain a 792-base-pair open reading frame, encoding a 264-amino-acid transmembrane glycoprotein with a predicted molecular mass of 29.4 kDa. The $swFc{\alpha}RI$ shares high amino acid sequence homology (>50%) with its counterparts from cattle, seal, and horse. Rosetting analysis confirmed that COS-7 cells transfected with an $swFc{\alpha}RI$ expression plasmid was able to combine with chicken erythrocytes sensitized with porcine IgA, but not IgG.

Use of Near-Infrared Spectroscopy for Estimating Lignan Glucosides Contents in Intact Sesame Seeds

  • Kim, Kwan-Su;Park, Si-Hyung;Shim, Kang-Bo;Ryu, Su-Noh
    • Journal of Crop Science and Biotechnology
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    • v.10 no.3
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    • pp.185-192
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    • 2007
  • Near-infrared spectroscopy(NIRS) was used to develop a rapid and efficient method to determine lignan glucosides in intact seeds of sesame(Sesamum indicum L.) germplasm accessions in Korea. A total of 93 samples(about 2 g of intact seeds) were scanned in the reflectance mode of a scanning monochromator, and the reference values for lignan glucosides contents were measured by high performance liquid chromatography. Calibration equations for sesaminol triglucoside, sesaminol($1{\rightarrow}2$) diglucoside, sesamolinol diglucoside, sesaminol($1{\rightarrow}6$) diglucoside, and total amount of lignan glucosides were developed using modified partial least square regression with internal cross validation(n=63), which exhibited lower SECV(standard errors of cross-validation), higher $R^2$(coefficient of determination in calibration), and higher 1-VR(ratio of unexplained variance divided by variance) values. Prediction of an external validation set(n=30) showed a significant correlation between reference values and NIRS estimated values based on the SEP(standard error of prediction), $r^2$(coefficient of determination in prediction), and the ratio of standard deviation(SD) of reference data to SEP, as factors used to evaluate the accuracy of equations. The models for each glucoside content had relatively higher values of SD/SEP(C) and $r^2$(more than 2.0 and 0.80, respectively), thereby characterizing those equations as having good quantitative information, while those of sesaminol($1{\rightarrow}2$) diglucoside showing a minor quantity had the lowest SD/SEP(C) and $r^2$ values(1.7 and 0.74, respectively), indicating a poor correlation between reference values and NIRS estimated values. The results indicated that NIRS could be used to rapidly determine lignan glucosides content in sesame seeds in the breeding programs for high quality sesame varieties.

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Comparative Growth Performance of Calves of Different Cattle Breeds Under a Feedlot Fattening System

  • Ahmad, Ijaz;Fiaz, Muhammad;Manzoor, Muhammad Nauman;Ahmad, Tanveer;Yaqoob, Muhammad;Jo, Ik Hwan
    • Journal of Animal Science and Technology
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    • v.55 no.6
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    • pp.539-543
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    • 2013
  • Male cattle calves (n=24), 9-12 months age, with an average body weight of 120 {\pm} 20 kg were fed total mixed rations (TMR) for 120 days to determine their growth performance. Animals were divided into four groups (six of each breed): Crossbred (Friesian${\times}$Sahiwal), Dhanni, Lohani, and Cholistani. The data obtained were analyzed using analysis of variance techniques under a completely randomized design. The average daily gain (ADG), feed efficiency (FE), and dressing percentage ranged from 639-892 g/d, 0.105-0.155 kg/kg, and 51.2-51.5%, respectively, in the different breeds. The ADG and FE did not differ between the Crossbred, Dhanni, and Lohani breeds, but these values were lower in Cholistani calves (P<0.05). The dressing percentage was similar in all breeds. The highest increase in body height was observed in Dhanni calves, but heart girth was obviously higher in Lohani calves. The feed cost per kg gain was higher for Cholistani calves but similar among Crossbred, Dhanni, and Lohani calves (P>0.05). In conclusion, Dhanni, Lohani, and Crossbred calves possess the promising potential for beef production under the rainfed (Barani) conditions of the Punjab.

A PHONEMIC ANALYSIS OF THE UNWRITTEN LANGUAGE OF THE PULANG TRIBE

  • Kang, Su-Hee
    • Proceedings of the KSPS conference
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    • 2000.07a
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    • pp.166-177
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    • 2000
  • The purpose of this study was to create letters for of nonliterary Pulang tribe in Thailand those who immigrant from China. illiterate Pulang tribe hand down their tradition by primary oral culture therefore their tradition can't initiate and keep, moreover, it may disappear throughout history. So it is expected to crusade against unlettered people. The scheme of research adopted in this study was a minority race who habitate at the northern Machan, Chiangrai in Thailand. It is not only analysis of language but also the eradication of literacy and the research based on linguistic, ethnolinguistic, and primary oral culture. Five Pulang people who live in that area were chosen for creating letters. By using the I. P. A., after each word was listen to their pronunciation one by one it was described and repeated this process several times; the material words and humanbody were pointed in front of them while other words were described by gesture. For final description, number of people were in the lineup for listening the sound of words and phrases to sentences. In the first stage, it was an analysis segmental of Pulang: vocoid, contoid and diphthong were described with each sample syllables and words. The suprasegmental were studied with intonation and juncture of the words in the second stage. Two words were compared and different meanings within their intonation and juncture were shown. At the end of this part, each case of phonemic or morphophonemics representation described the juncture in the words. In the third stage, minimal pairs were analyzed with vowels and consonants and described in free variation based on words. In the last stage, syllable structure in open syllable and closed syllable was studied and then each syllable of its structure was analyzed with samples. There were thirty-two phonemes in apong Pulang as follows: seven vocoids; a, i, e, o, u, ${\ae}$, and $\wedge$, one diphthong; wu, 24 contoids; b, c, d, f, g, h, j, k, k, 1, m, n, ${\eta}, {\;}p^{h}$, p, p, r, s, s, sh, t, t, w, and y. Their pronunciations of p, s, d, $p^{h}$, j, and t are frequently used in speech and are unique in triphthong. Moreover, most of the words used initial and final consonant cluster.

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Isolation and characterization of Brcpi1 gene encoding phytocystatin from chinese cabbage (Brassica rapa L.) seedlings (배추 유래 phytocystatin 유전자, Brcpi1의 분리 및 발현특성 분석)

  • Jung, Yu-Jin;Cho, Yong-Gu;Kang, Kwon-Kyoo
    • Journal of Plant Biotechnology
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    • v.36 no.4
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    • pp.407-414
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    • 2009
  • A cDNA clone encoding phytocystatin was isolated from Brassica rapa seedlings, through rapid amplification of cDNA ends (RACE). This gene (name as Brcpi1; GenBank accession no.: EF079953) had a total length of 881 bp with an open reading frame of 609 bp, and encoded predicted polypeptide of 203 amino acid (aa) residues including a putative N-terminal signal peptide. Other relevant regions found its sequence included the G and PW conserved aa motifs, and the consensus LARFAV sequence for phytocystatins and the reactive site QVVAG. The BrCPI1 protein shared 95, 94, 81, 80 and 78% identity with other CPI proterins isolated from Brassica oleracea (BoCPI-1), Arabidopsis thaliana (AtCY SB), Glycine max (GmCPI), Oryza sativa (OsCYS-2) and Zea may (ZmCPI) at amino acid level, respectively. Southern blot analysis showed that Brcpi1 was a low copy gene. Expression pattern analysis revealed that Brcpi1 was a tissue-specific expressing gene during reproductive growth and strongly expressed at mature seedling stages. Furthermore, overexpression of Brcpi1 in transgenic Arabidopsis was enhanced tolerance to salt and cold stresses. Meanwhile the juvenile seedling of Brcpi1 transgenic plants was not affected by various concentrations ABA in MS medium. Taken together, the results showed that Brcpi1 functioned as a cysteine protease inhibitor and it exhibited a protective agent against diverse types of abiotic stress, which induced this gene in a tissue- and stress-specific manner.