• Journal of Microbiology and Biotechnology
• /
• 제8권1호
• /
• pp.34-41
• /
• 1998

A method was developed for the controlled expression of an antimicrobial peptide magainin in Escherichia coli. A series of concatemeric magainin genes was constructed with a gene amplification vector, and fused to the 3'end of malE gene encoding the affinity ligand, E. coli maltose-binding protein (MBP). The construct directed the synthesis of the fusion protein with the magainin polypeptide fused to the C-terminus of MBP. The fusion protein was expressed in a tightly regulatable expression system which was under the control of an invertible promoter. The MBP-fused magainin monomer was expressed efficiently. However, the expression level of the MBP-fused magainin in E. coli decreased with the increasing size of multimers possibly because of the transcription and translation inhibition by the multimeric peptides. After purification using an amylose affinity column, the fusion protein was digested by factor Xa at a specific cleavage site between the monomers. The recombinant magainin had an antimicrobial activity identical to that of synthetic magainin. This experiment shows that a biologically active, antimicrobial peptide magainin can be produced by fusing to MBP, along with a promoter inversion vector system.

• KSII Transactions on Internet and Information Systems (TIIS)
• /
• 제8권1호
• /
• pp.196-212
• /
• 2014

Facial expression recognition is an important part in emotional interaction between human and machine. This paper proposes a facial expression recognition approach based on multi-classifier fusion with stacking algorithm. The kappa-error diagram is employed in base-level classifiers selection, which gains insights about which individual classifier has the better recognition performance and how diverse among them to help improve the recognition accuracy rate by fusing the complementary functions. In order to avoid the influence of the chance factor caused by guessing in algorithm evaluation and get more reliable awareness of algorithm performance, kappa and informedness besides accuracy are utilized as measure criteria in the comparison experiments. To verify the effectiveness of our approach, two public databases are used in the experiments. The experiment results show that compared with individual classifier and two other typical ensemble methods, our proposed stacked ensemble system does recognize facial expression more accurately with less standard deviation. It overcomes the individual classifier's bias and achieves more reliable recognition results.

• Journal of Microbiology
• /
• 제34권2호
• /
• pp.137-143
• /
• 1996

We isolated a promoter inducible by paraquat, a superoxide-generating agent, from Escherichia coli using a promoter-probing plasmid pRS415. From sequence analysis we found out the promoter is for fpr ENCODING nadph : ferredoxin oxidoreductase. We constructed on operon fusion of lacZ gene with fpr promoter to monitor the expression of the gene in the single-copy state. LacZ expression generators, menadione and plumbagin, also induced the expression of .betha.-galactosidase in the fusion strain. On the other hand, no significant induction was observed by treatment with hydrogen peroxide, ethanol, and heat shock. Induction of .betha.-galactosidase was significantly reduced by introducing a .DELTA. sox 8 :: cat of soxS3 :: Tn10 mutation into the fusion strain, indicating that fpr gene is a member of the soxRS regulon. The transcriptional start site was determined by primer extension analysis. Possible roles of fpr induction in superoxide stress were discussed.

• Journal of Applied Biological Chemistry
• /
• 제43권1호
• /
• pp.1-4
• /
• 2000

Chloramphenicol acetyl CoA transferase (CAT) and angiotensin- converting enzyme inhibitory peptide (ACEI) were fused to C-terminal region of rice ubiquitin to examine the level of transcripts or enzyme activities in yeast. When two chimeric genes under an inducible Gall promoter control were transformed into Saccharomyces cerevisaie, both CAT and ACE inhibitory activities were enhanced by three to four-fold as compared to those containing no ubiquitin gene. However, the levels of transcripts of ubiquitin fused and un fused genes were not significantly different each other. Therefore, it was suggested that the expression of foreign genes was post-transcriptionally enhanced by fusion of plant ubiquitin in heterologous organisms such as yeast.

• 미생물학회지
• /
• 제29권4호
• /
• pp.215-220
• /
• 1991

MudJ(Km.lac) operon fusions were used in the identification of osmotic-inducible genetic(osi) loci in Salmonella typhimurium. Expression of osi::lacZ(osi5001, 5027) genes were dramatically induced 39-189 fold when the osmolarity was increased. Seven osm::lacZ genes were constituvely expressed under both low and high osmotic strength. The osi5001::lacZ fusion strains showed the enhanced osmotolerance and the reduced expression of the osi5001::lacZ in the presence of 1mM proline or betaine as osmoprotectants. Four osmotic inducible genetic loci were mapped into 36 (YK531), 44 (YK504), 57 (YK501) and 84 (YK528) map unit by testing the cotransduction frequency.

• 한국지능시스템학회논문지
• /
• 제18권1호
• /
• pp.20-26
• /
• 2008

지능형 로봇이나 컴퓨터가 일상생활 속에서 차지하는 비중이 점점 높아짐에 따라 인간과의 상호교류도 점점 중요시되고 있다. 이렇게 지능형 로봇(컴퓨터) - 인간의 상호 교류하는데 있어서 감정 인식 및 표현은 필수라 할 수 있겠다. 본 논문에서는 음성 신호와 얼굴 영상에서 감정적인 특징들을 추출한 후 이것을 Bayesian Learning과 Principal Component Analysis에 적용하여 5가지 감정(평활, 기쁨, 슬픔, 화남, 놀람)으로 패턴을 분류하였다. 그리고 각각 매개체의 단점을 보완하고 인식률을 높이기 위해서 결정 융합 방법과 특징 융합 방법을 적용하여 감정 인식 실험을 하였다. 결정 융합 방법은 각각 인식 시스템을 통해 얻어진 인식 결과 값을 퍼지 소속 함수에 적용하여 감정 인식 실험을 하였으며, 특징 융합 방법은 SFS(Sequential Forward Selection) 특징 선택 방법을 통해 우수한 특징들을 선택한 후 MLP(Multi Layer Perceptron) 기반 신경망(Neural Networks)에 적용하여 감정 인식 실험을 실행하였다. 그리고 인식된 결과 값을 2D 얼굴 형태에 적용하여 감정을 표현하였다.

• 한국수정란이식학회:학술대회논문집
• /
• 한국수정란이식학회 2004년도 제4회 발생공학 국제심포지움 및 학술대회
• /
• pp.115-115
• /
• 2004

• 한국생물공학회:학술대회논문집
• /
• 한국생물공학회 2001년도 추계학술발표대회
• /
• pp.225-228
• /
• 2001

RhRnBp and Peysn5 are the proteins related to carbohydrate synthesis. RhRnBp originated form human was expressed as inclusion body in E. coli. Peysn5 originated form actinomadura was expressed as low level and inclusion body in E. coli. Ub, Trx, MalE and NusA is used as fusion partner to RhRnBp and Peysn5. The solubility of all fusion protein is NusA > MalE> Trx > Ub. Expression level of RhRnBp fusions in $37^{\circ}C$ is higher than that in $25^{\circ}C$. However in the case of Peysn5. Expression levels in $25^{\circ}C$ were higher. MalE fusion had highest activity in RhRnBp fusions. There were no activity in Peysn5.

• Bulletin of the Korean Chemical Society
• /
• 제32권12호
• /
• pp.4337-4340
• /
• 2011

The vitamin D receptor binding peptide, VDRBP, was overexpressed as a fused form with the ubiquitin molecule in Rosetta(DE3)pLysS, a protein production strain of Escherichia coli harboring an induction controller plasmid. The fusion protein was bound to the immobilized metal ions, and the denaturation and renaturation of the fusion protein were performed as a part of the purification procedure. After the elution of the fusion protein, the peptide hormone was released from its fusion partner by using yeast ubiquitin hydrolase (YUH), and subsequently purified by reverse phase chromatography. The purity of the resulting peptide fragment was checked by MALDI-TOF mass and NMR spectroscopy. The final yields of the target peptide were around 5 and 2 mg per liter of LB and minimal media, respectively. The recombinant expression and purification of this peptide will enable structural and functional studies using multidimensional NMR spectroscopy and X-ray crystallography.

• 한국실내디자인학회논문집
• /
• 제29호
• /
• pp.3-10
• /
• 2001

In recent years when the importance of public communication is more emphasized, it has to be noticed that we are now living in the public-initiated age of rhetoric resulted from rapid development of broadcast media. Therefore, the purpose of this treatise is to examine the structural characteristics in the fusion design in order to anaylize the rhetorical expressions as symbols of communication. One of those is intertextuality that expresses metaphorically by bleding and borrowing codes and another is hypertextual space where various texts twines around each other making brand-new and diverse organizations, as the combined allegory with a number of hidden expression. The fusion design may be understood as the Intention to attract gaze to visual messages and as the rhetorical expressions based on compounding aesthetic codes by producing new cultural meanings. If fusion, a phenomenon which represents the 21st century, not a passing fad of cultural mixture, is ready to take the initiative, the design has to be groped for that creates a synergy effect.