• BMB Reports
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• 제41권8호
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• pp.575-580
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• 2008

Thymocyte-specific transcriptional regulatory systems can be used to better understand the relationship between transcription and V(D)J recombination during early T cell development. In this study, we generated transgenic mice expressing the transactivator Gal4-VP16 or the Gal4 DNA binding domain (Gal4-DBD) under the control of the lck proximal promoter, which is only active in immature thymocytes. From these studies Gal4-VP16 and Gal4-DBD expression was shown to significantly alter thymic cellularity and differentiation without significantly changing the $CD3^+$ thymocyte distribution. Furthermore, the presence of Gal4-VP16 or Gal4-DBD in the transgenic thymocytes retarded the mobility of the Gal4 DNA binding motif as determined by a gel mobility shift assay, suggesting that the developmental alteration did not affect the functional property of the transgenic proteins. These results indicated that lck promoter-driven Gal4-VP16 or Gal4-DBD expression did not affect $CD3^+$ mature thymocytes, thus this system can be applied to study transcriptional regulation of transresponder genes in bigenic mouse model thymocytes.

• 한국의류학회지
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• 제31권4호
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• pp.531-539
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• 2007

The purpose of this article was to determine how the current auto mechanic's coverall restricts movements, to develop prototype coverall, and finally, to improve working efficiency. To investigate the problem and complaint of current coveralls, 35 mechanics were interviewed. Video captured method was used to analyze the representative movement. Based on the previous investigation, the prototype coverall was developed. Pleats and band were inserted on each side of the back, under the armhole, and the back waist area for flexibility. To analyze the mobility of current and prototype coveralls objectively, range-of-motion of selected movements was measured by Leighton flexometer and goniometer. Also, wearer acceptability scale was tested to investigate subjective preference of current and prototype coveralls. As subjects, 10 auto mechanics were participated. Order of testing was randomized. ANOBA and LSD were used for statistical analysis. As a results, prototype coverall was significantly different from current coverall at all test movements. Also, prototype coverall was significantly different from current coverall at 13 scales among 23 subjective scales. The results identified that prototype coverall design improved auto mechanic's body movement and subjective acceptability.

• Integrative Medicine Research
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• 제5권3호
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• pp.176-181
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• 2016

Recent studies have focused on evidence-based interventions to prevent mobility decline and enhance physical performance in older adults. Several modalities, in addition to traditional strengthening programs, have been designed to manage age-related functional decline more effectively. In this study, we reviewed the current relevant literatures to assess the therapeutic potential of eccentric exercises for age-related muscle atrophy (sarcopenia). Age-related changes in human skeletal muscle, and their relationship with physical performance, are discussed with reference to in vitro physiologic and human biomechanics studies. An overview of issues relevant to sarcopenia is provided in the context of the recent consensus on the diagnosis and management of the condition. A decline in mobility among the aging population is closely linked with changes in the muscle force-velocity relationship. Interventions based specifically on increasing velocity and eccentric strength can improve function more effectively compared with traditional strengthening programs. Eccentric strengthening programs are introduced as a specific method for improving both muscle force and velocity. To be more effective, exercise interventions for older adults should focus on enhancing the muscle force-velocity relationship. Exercises that can be performed easily, and that utilize eccentric strength (which is relatively spared during the aging process), are needed to improve both muscle force and velocity.

• 한국표면공학회지
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• 제56권1호
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• pp.1-11
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• 2023

Lubricant-impregnated nanoporous surfaces (LIS), which is created by impregnating water-immiscible oil into nanoporous surface structure, have been explored considering wide range of application fields. Due to the lubricant impregnated in nanoporous structure, the surface shows extreme de-wetting with a high mobility of water droplets, so that various functionalities can be realized. The lubricant layer inhibits the contact of corrosive media to porous structure as well as metal substrate, thus the surface improves the corrosion resistance. The water on the surface freeze without any contact to solid porous structure, showing a low ice adhesion for de-icing an anti-icing. The extremely high mobility of water droplets on lubricant-impregnated porous surfaces also contributes the enhancement of condensation heat transfer as well as water harvesting from fog and moisture. Moreover, the bacteria adhesion on metal surface forming biofilms causing serious hygiene issues can be inhibited on the lubricantimpregnated surfaces. Despite of such superior functionalities, the lubricant-impregnated porous surface has a limitation of lubricant depletion by external flow of fluids. Therefore, extensive efforts to improve the durability of lubricant-impregnated surface are required for practical applications.

• Journal of Information Technology Applications and Management
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• 제31권3호
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• pp.39-52
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• 2024

The development of autonomous driving technology is expected to bring about a major change in the mobility rights of the transportation vulnerable. It is very important to identify user requirements in developing autonomous vehicles and service technologies for the transportation vulnerable. User requirements were derived for the wheelchair disabled and the walking disabled. Through focus interviews, a total of 58 requirements were derived for wheelchair-using disabled people and 53 requirements for walking disabled people. A Kano survey was conducted on 33 wheelchair disabled and 34 walking disabled. After that, the quality types of functional requirements in terms of autonomous vehicles and service environment development were analyzed using the Kano model. Priority analysis was conducted on the functions required by the wheelchair disabled and the walking disabled. The results of this study can be used as basic data to determine the priorities of user function requirements in the early stages of autonomous vehicle and service technology development.

• 복식문화연구
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• 제15권5호
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• pp.796-809
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• 2007

The viewpoint of growth pursuit is changed to that of life quality's and happiness' pursuit by modern people. Modern people in digital environment, tired of fast-changing and oppressive daily life, prefer well-being trend providing tranquility and relaxation. Well-being is related to digital, because negative influences of digital lead human-oriented, environment-friendly well-being design. Therefore the purpose of this study is to research and analyze the relation of well-being fashion design and digital environment through case study in contemporary well-being fashion design. The results of this study are as follows: 1. This study shows that the attributes of digital environment make up function-intensive, interactivity, mobility & nomad, human & emotion-oriented and environment-friendly. And this study shows the design characteristics of digital environment consist of function-intensive design by digital convergence, open structure design for interactivity, modular design for mobility & nomad, emotion-oriented & multi-sensory design and environment- friendly design. 2. Function-intensive design in modem well-being fashion composed of vogue of caports style(casual+sports), practical use of multi-functional new-healthy textiles, and popularity of many style's mixture at once. Open structure design consist of wrapover design, use of transparent materials. Modular design are layered styling, practical use of zipper and velcro. Emotion-oriented & multi-sensory design are constitute of fad of wrinkle materials, application of bright & vivid tone, personal color and family look. Environment-friendly design in contemporary well-being fashion are comprised in use of natural color, re-advent of natural floral motif and eco-design.

• 한국ITS학회 논문지
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• 제6권1호
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• pp.58-71
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• 2007

교통약자 즉, 장애인과 노약자의 자립생활과 사회통합을 위한 장애물 없는 생활환경(Barrier-Free)의 실현은 건축, 도시, 교통, 도로 등 통합적인 측면에서 생활환경의 설계기준 재정비 등 하드웨어적인 측면과 함께 사회활동에 필요한 편의시설의 다양한 정보접근과 이용 등 소프트웨어적인 측면이 동시에 충족되어야 하며, 소프트웨어적인 측면은 편의시설의 이용성을 제고하는데 있어 매우 중요한 문제이다. 이러한 측면에서 편의시설의 이용성 향상을 위해 유비쿼터스 기술 등 정보통신기술을 활용한 지원시스템 구축이 필요하다. 대표적 교통약자인 장애인 노약자가 사회생활을 하기 위하여 주거지를 넘어 다른 장소로 이동하고자 할 경우에도 이들의 목적을 달성하기에 어려움이 없도록 정보를 제공하고 지원하는 시스템이 필요하다. 장애인, 노약자의 행태조사 및 요구조사를 통한 요구사항분석과 장애인, 노약자 중심의 생활 및 이동지원시스템관련 국외 연구동향 및 관련 기술요소파악 및 시스템 요구조건과 유즈케이스를 도출하였다.

• The Korean Journal of Pain
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• 제21권3호
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• pp.197-201
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• 2008

Background: Kyphoplasty is a minimally invasive procedure that can stabilize osteoporotic and neoplastic vertebral fractures. We retrospectively evaluated the clinical outcomes of kyphoplasty for the treatment of vertebral compression fractures in cancer patients. Methods: We reviewed the clinical data of 27 cancer patients who were treated with kyphoplasty (55 vertebral bodies) between May 2003 and Feb 2008. The clinical parameters, using a visual analog 10 point scale (VAS) and the mobility scores, as well as consumption of analgesic, were evaluated preoperatively and at 1 week after kyphoplasty. Results: A total 55 cases of thoracic and lumbar kyphoplasties were performed without complications. The mean age of the patients was 66 years. All the patients experienced a significant improvement in their subjective pain and mobility immediately after the procedures. The pain scores (VAS), mobility scores and other functional evaluations using the Oswestry disability score and the SF-36 showed significant differences between the pre- and postoperational conditions. Conclusions: Kyphoplasty is an effective, minimally invasive procedure that can relieve the pain of patients with vertebral compression fractures and these fractures are the result of metastasis.

• 한국의류산업학회지
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• 제13권5호
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• pp.759-768
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• 2011

This study is designed to understand current wearing conditions of air force mechanic parkas and evaluate their functionality by examining the wearing conditions and wearers' subjective assessment. By doing so, it also intends to identify issues that require improvements; and to provide basic data for future development of air force mechanic parkas. A survey was conducted as a study methodology, and the collected 1,628 questionnaires were analyzed. Findings of this study are as follows. 1. In the usability assessment, it was found that visibility of body sides was needed to be enhanced since reflective tapes were attached only to the front and back of the body; pockets were too big, but not convenient to keep mechanic tools; the design of hiding hood was not suitable for water-proof clothing; and a new design of size-controllable hood was called for since the hood blocked eye sight. With respect to the fabric, dissatisfaction was identified with durability, cold-proof and fire-proof features. 2. In the mobility assessment, respondents showed low satisfaction with collar heights and neck girths. Mobility score was over moderate level on average, except the parka length that obstructs wearers' movement, and neck and collar size. 3. In the wearability assessment, respondents presented moderate satisfaction with pleasantness, weight, pressure and dampness. However, insulation was found unsatisfactory. Based on the result, this study proposed improvement plans on design, pattern and fabric; and is expected to serve as basic data for developing more effective and functional air force mechanic parkas.

• Journal of Plant Biotechnology
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• 제45권4호
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• pp.322-327
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• 2018

Post-translational modification of proteins regulates signaling cascades in eukaryotic system, including plants. Among these modifications, phosphorylation plays an important role in modulating the functional properties of proteins. Plants perceive environmental cues that directly affect the phosphorylation status of many target proteins. To determine the effect of environmentally induced phosphorylation in plants, in vivo methods must be developed. Various in vitro methods are available but, unlike in animals, there is no optimized methodology for detecting protein phosphorylation in planta. Therefore, in this study, a robust, and easy to handle Phos-Tag Mobility Shift Assay (PTMSA) is developed for the in vivo detection of protein phosphorylation in plants by empirical optimization of methods previously developed for animals. Initially, the detection of the phosphorylation status of target proteins using protocols directly adapted from animals failed. Therefore, we optimized the steps in the protocol, from protein migration to the transfer of proteins to PVDF membrane. Supplementing the electrophoresis running buffer with 5mM $NaHSO_3$ solved most of the problems in protein migration and transfer. The optimization of a fast and robust protocol that efficiently detects the phosphorylation status of plant proteins was successful. This protocol will be a valuable tool for plant scientists interested in the study of protein phosphorylation.