• Asian Pacific Journal of Cancer Prevention
• /
• 제16권17호
• /
• pp.7529-7534
• /
• 2015

Background: The epidermal growth factor (EGF) plays important roles in non-small cell lung cancer (NSCLC) susceptibility and functional polymorphism in the EGF (+61A/G) gene has been linked to increased risk of NSCLC. This study aimed to evaluate the role of the EGF +61A/G polymorphism in risk of NSCLC adenocarcinoma (ADC) occurrence and survival in an Indian population. Materials and Methods: This casecontrol study included 100 histopathologically confirmed NSCLC (ADC) patients and 100 healthy controls. EGF (A61G) was genotyped by AS-PCR to elucidate putative associations with clinical outcomes. The association of the polymorphism with the survival of NSCLC patients was estimated by Kaplan-Meier curves. Results: It was found that EGF 61AG heterozygous and GG homozygous genotype is significantly associated with increased risk of NSCLC (ADC) occurrence compared to AA genotype, [OR 2.61 (1.31-5.18) and 3.25 (1.31-8.06), RR 1.51(1.15-2.0) and 1.72 (1.08-2.73) and RD 23.2 (6.90-39.5) and 28.53(7.0-50.1) for heterozygous AG (p=0.005) and homozygous GG (p=0.009)]. Patients homozygous for the G allele exhibited a significantly poor overall survival. The median survival time for patients with EGF 61 AA, AG, and GG genotypes was 10.5, 7.4, and 7.1 months (p=0.02), respectively. NSCLC (ADC) patients with GG + AG exhibited 7.3 months median survival compared to the AA genotype (p=0.009). Conclusions: The present study revealed that the EGF A61G genotype may be a novel independent prognostic marker to identify patients at higher risk of occurrence and an unfavourable clinical outcome.

• 한국가정과학회지
• /
• 제7권3호
• /
• pp.15-33
• /
• 2004

A precise understanding of the human form in static pose serves as the basis of designing clothing. When the human body is in motion, however, even an article of clothing designed to fit the human form in static pose can pull and change, thus restricting the body. In order to increase the fit of the clothing, which may be termed the second skin, its form and measurements therefore must be determined in correlation not only with the formal characteristics of the human body, in static pose but also with its functional characteristics in motion, as caused by the movements of the human body. In this study, the motion factor was selected as the primary basis for designing slacks with good fit in both static and moving states. By indentifying the areas in which lower limb movement cause significant changes in body surface lines, we suggest several application methods for designing slacks. Using unmarried female university students aged 18 - 24 as subjects, a total of 32 body surface categories (15 body surface lines and 17 body surface segment lines) were measured in one static and 9 movement poses. In particular, expansion and contraction levels and rates were measured and used in the analysis. The analysis first involved the calculation of the average measurement per body part in body surface line in static pose as well as of the average expansion and contraction levels and rates in 9 lower limb movements. Two-way MANOVA and multiple comparison analysis (Tukey) were conducted on movements and individual somatotypes regarding measurement per body part and expansion and contraction rates. Body parts whose measurements of body surface lines differed significantly in body surface line in static pose versus in movement were then identified. The results of this study are as follows. First, changes in body surface lines caused by lower limb movements were significant in all body surface lines of the lower trunk, both horizontal and vertical, with the exception of abdomen girth, midway thigh girth, ankle girth, hip length, and posterior knee girth. Second, significantly expanded 10 body surface lines in moving pose were detected and illustrated in table 4. These body parts should be studied in designing or pattern designing, especially for close-fitting pants, in using stretch fabric, and in sensory evaluation of good fit during movement.

• 한국작물학회:학술대회논문집
• /
• 한국작물학회 2017년도 9th Asian Crop Science Association conference
• /
• pp.262-262
• /
• 2017

Brown rice contains a variety of nutritional and bio-functional components compared to milled rice. However, low consumer preference for steamed brown rice and short shelf life has been implicated as deterrents to the direct consumption of brown rice. This study was undertaken to determine the effect of degree of milling (DOM) on pasting and aging properties of brown rice cultivar. Short-grain (Japonica type) brown rice cv. Boseogchal (BSC), Baegjinju (BJJ), and Hiami (HIAM) were evaluated every month during 4 months storage. Rice cultivar were categorized into waxy (BSC), medium-waxy (BJJ), and non-waxy (HIAM) of which amylose contents were 6.81%, 10.06%, and 16.69~16.72%, respectively. Peak viscosity was the highest in BJJ followed by HIAM, and BSC, showing peak viscosity was lower at low temperature storage. At storage at room temperature from 60 to 120 days, the FFA content significantly increased in the rice samples with DOM1% followed by 3% and 5%, while not significant increase in DOM0% brown rice: at 120 days, the FFA ranged in 28.35~41.48, 111.99~130.06, 76.87~91.99, and 47.13~62.37 mg KOH/100g for DOM 0%, 1%, 3% and 5%, respectively. It indicated that the fat rancidity was the least in the order of DOM 0%, 5%, 3% and 1%. Storage at low temperature could reduce fat hydrolysis with lower FFA release, but rice grain with 1% MD also released more FFA compared to the other samples. It could be assumed that the most outer bran layer, approximately 1~3%, could provide a strong protection from fat rancidity. Pasting viscosity of rice samples also increased with increasing DOM rate. Results indicated that DOM was the most significant factor for lipid rancidity during storage, resulting in high FFA formation. It suggested that brown rice with DOM 0% and/or more than DOM 5% could provide the best shelf life.

• 한국작물학회:학술대회논문집
• /
• 한국작물학회 2017년도 9th Asian Crop Science Association conference
• /
• pp.140-140
• /
• 2017

Nutritious and functional foods from crop have received great attention in recent years. Colored-grain wheat contains high phenolic compound and a large number of flavonoid. The anthocyanin and polyphenolic synthesis and accumulation is generally stimulated in response to biotic or abiotic stresses. Here, we analyzed genome wide transcripts in seedling of colored-grain wheat response to ABA and PEG treatment. About 900 and 1500 transcripts (p-value < 0.05) from ABA and PEG treatment were aligned to IWGSC1+popseq DB which is composed of over 110,000 transcripts including 100,934 coding genes. NR protein sequences of Poaceae from NCBI and protein sequence of transcription factors originated from 83 species in plant transcription factor database v3.0 were used for annotation of putative transcripts. Gene ontology analysis were conducted and KEGG mapping was performed to show expression pattern of biosynthesis genes related in flavonoid, isoflavonoid, flavons and anthocyanin biopathway. DroughtDB (http://pgsb.helmholtz-muenchen.de/droughtdb/) was used for detection of DEGs to explain that physiological and molecular drought avoidance by drought tolerance mechanisms. Drought response pathway, such as ABA signaling, water and ion channels, detoxification signaling, enzymes of osmolyte biosynthesis, phospholipid metabolism, signal transduction, and transcription factors related DEGs were selected to explain response mechanism under water deficit condition. Anthocyanin, phenol compound, and DPPH radical scavenging activity were measured and antioxidant activity enzyme assays were conducted to show biochemical adaptation under water deficit condition. Several MYB and bHLH transcription factors were up-regulated in both ABA and PEG treated condition, which means highly expressed MYB and bHLH transcription factors enhanced the expression of genes related in the biosynthesis pathways of flavonoids, such as anthocyanin and dihydroflavonols in colored wheat seedlings. Subsequently, the accumulation of total anthocyanin and phenol contents were observed in colored wheat seedlings, and antioxidant capacity was promoted by upregulation of genes involved in maintaining redox state and activation of antioxidant scavengers, such as CAT, APX, POD, and SOD in colored wheat seedlings under water deficit condition. This work may provide valuable and basic information for further investigation of the molecular responses of colored-grain wheat to water deficit stress and for further gene-based studies.

• 원예과학기술지
• /
• 제33권4호
• /
• pp.575-584
• /
• 2015

건조 스트레스는 작물의 생존과 생산성을 결정하는데 매우 중요한 환경요인이다. 본 연구의 목적은 배추에서 신규 건조 스트레스 저항성 유전자를 동정 검정하는 것이다. 건조 스트레스 하에서 생육된 지부('Chiifu') 배추를 이용하여 제작된 KBGP-24K 마이크로어레이 데이터 분석을 통해 738개의 건조 반응 유전자 중 기능은 밝혀져 있지 않지만, 건조 스트레스 하에서 발현량이 6배 이상 크게 증가한 1개의 유전자를 선발하여 BrDSR(B. rapa Drought Stress Resistance)이라 명명하였다. 이의 검정을 위해 내혼계배추('CT001')에서 BrDSR을 동정한 결과 438bp의 오픈리딩프레임과 145개의 아미노산을 가지고 있음을 확인하였고, 동정된 완전장의 cDNA 염기서열은 형질전환용 과발현 vector인 'pSL100' 제작에 이용하였다. BrDSR이 식물체에서 건조 스트레스 저항성을 향상시켜줄 수 있는지 분석하기 위해 담배 형질전환을 수행하였다. PCR과 DNA 블롯 분석으로 선발된 T1 세대 담배 형질전환체들을 대상으로 quantitative real-time RT PCR 분석을 수행한 결과, 형질전환체의 BrDSR 발현량은 비형질 전환체 보다 2.6배까지 증가하였다. 또한 건조처리 10일째 수행한 표현형 분석에서 BrDSR이 발현되는 담배 형질전환체들이 비형질전환체들 보다 우수한 건조 저항성을 보였다. 연구 결과들을 종합하면 BrDSR은 건조 스트레스 하에서 식물의 생장과 생존에 효과적인 저항성 기능을 할 것으로 기대된다.

• 감성과학
• /
• 제19권1호
• /
• pp.71-82
• /
• 2016

소비자들은 구두를 단순히 발을 보호하고 편안하게 해주는 역할 뿐만 아니라 장식과 자아 이미지를 표현해주는 패션제품으로써 인지하는 경향이 있다. 온라인 시장 분석에서 구두가 소비자들의 일상생활에서 중요한 제품이라고는 하였지만 인터넷 구두 시장을 조사한 연구는 드물다. 본 연구는 인터넷 구두시장에서 구두 e-충동구매과정에 대한 쇼핑성향, 마케팅 자극, 소비자들이 인지하는 위험지각의 영향에 초점을 맞추어 조사하였다. 최근 6개월 동안 구두 e-충동구매를 경험한 적이 있는 대학생들 총 408명을 대상으로 자기기술형식의 자료를 수집하였다. 결과에 의하면, 쾌락적 쇼핑성향이나 브랜드 쇼핑성향이 높은 소비자들은 인터넷에서 제공하는 마케팅 자극(판매촉진 자극이나 제품자극)을 더 많이 인지하였고, 반면 경제적 쇼핑성향의 소비자들은 마케팅 자극을 더 낮게 인지하였으나 기능적인 위험은 더 높게 지각하는 것으로 나타났다. 구두의 경우, 마케팅 자극이 강할수록 e-충동구매는 증가하였으나 소비자들이 지각하는 위험은 e-충동구매에 아무런 영향을 미치지 않았다. 이러한 연구결과는 소비자의 쇼핑성향에 영향을 받는 마케팅 자극이 구두 e-충동구매를 유발하는데 중요한 요인임을 제안한다.

• 한국축산식품학회지
• /
• 제37권6호
• /
• pp.940-947
• /
• 2017

Goat milk has a protein composition similar to that of breast milk and contains abundant nutrients, but its use in functional foods is rather limited in comparison to milk from other sources. The aim of this study was to prepare a goat A2 ${\beta}$-casein fraction with improved digestibility and hypoallergenic properties. We investigated the optimal conditions for the separation of A2 ${\beta}$-casein fraction from goat milk by pH adjustment to pH 4.4 and treating the casein suspension with calcium chloride (0.05 M for 1 h at $25^{\circ}C$). Selective reduction of ${\beta}$- lactoglobulin and ${\alpha}_s$-casein was confirmed using sodium dodecyl sulphate-polyacrylamide gel electrophoresis and reverse-phase high-performance liquid chromatography. The hypoallergenic property of A2 ${\beta}$-casein fraction was examined by measuring the release of histamine and tumor necrosis factor alpha from HMC-1 human mast cells exposed to different proteins, including A2 ${\beta}$-casein fraction. There was no significant difference in levels of both indicators between A2 ${\beta}$-casein treatment and the control (no protein treatment). The A2 ${\beta}$-casein fraction is abundant in essential amino acids, especially, branched-chain amino acids (leucine, valine, and isoleucine). The physicochemical properties of A2 ${\beta}$-casein fraction, including protein solubility and viscosity, are similar to those of bovine whole casein which is widely used as a protein source in various foods. Therefore, the goat A2 ${\beta}$-casein fraction may be useful as a food material with good digestibility and hypoallergenic properties for infants, the elderly, and people with metabolic disorders.

• 콘크리트학회논문집
• /
• 제24권5호
• /
• pp.543-551
• /
• 2012

도로포장의 내구 연한이 기대수명에 비하여 짧아지고 있어 최근 다기능 복합포장 개발 연구가 진행중이다. 이 연구에서는 아스팔트 표층을 구성하기 위한 빈배합 콘크리트 기층재료로 활용하기 위하여 재료적인 측면을 개선시키는 방안과 섬유보강재를 추가하여 포장체를 보강하는 연구방안으로 역학적 특성을 분석 연구를 수행하였다. 빈배합 콘크리트의 경우 그 특성상 다짐의 수준이 강도 등의 특성 발현에 많은 영향을 미치게 된다. 하지만 현재 국내에서는 기층의 다짐관리에 관한 세부적인 기준이 미비하므로 자체적으로 다짐방법 및 수준을 정하여 제시하였다. 실내 및 현장 실험 결과 압축강도기준은 재령 7일에 5 MPa로서 모든 빈배합 콘크리트의 변수가 이를 충분히 만족시키는 것으로 나타났으며 현장배합 강도의 경우 일반적인 수화반응에 의한 강도의 증진효과 보다는 롤러의 다짐에너지에 의한 강도의 증진효과가 지배적으로 작용하였을 것으로 판단된다.

• 대한치과보철학회지
• /
• 제56권3호
• /
• pp.235-242
• /
• 2018

임플란트 피개의치에서 치주적, 보철적 유지관리는 보철물과 임플란트의 장기적 성공에 매우 중요한 요인이다. 유지관리의 실패는 임플란트 주위 골소실 및 보철물이나 구성품의 파절 및 마모로 인해 보철적 실패를 유발할 수 있다. 이 증례에서는 유지관리 실패로 파절된 임플란트 피개의치의 재치료를 위해 기존의 골드 milled bar를 코발트 크롬 milled bar로 외형에 방해되지 않는 형태로 재제작하였고, 하악의 기존 고정성보철은 임플란트 주변 골흡수와 환자의 전신적 문제로 인해 전략적으로 임플란트를 선택하여 두 개의 임플란트에 CM LOC을 연결하여 임플란트 피개의치를 제작하여 재수복하였다. 결과적으로 환자의 위생관리를 수월하게 하는 디자인의, 기능적 심미적으로 장기간 유지관리에 유리한 보철물로 수복하였고 정기적인 유지관리를 시행하였다.

• 대한의생명과학회지
• /
• 제13권3호
• /
• pp.197-206
• /
• 2007

P2X receptors are membrane-bound ion channels that conduct $Na^+,\;K^+$, and $Ca^{2+}$ in response to ATP and its analogs. There are seven subunits identified so far ($P2X_1-P2X_7$). $P2X_2$ receptors are known to be expressed in a wide range of organs including brains and adrenal grands. PC12 cells are originated from adrenal grand and differentiated by nerve growth factor or pituitary adenylate cyclase activating poly peptide (PACAP). Previous studies indicate that $P2X_2$ receptor activation in PC12 cells couples to $Ca^{2+}-dependent$ release of catecholamine and ATP. It is known that acidic pH potentiates ATP currents at $P2X_2$ receptors. This leads to a hypothesis that $P2X_2$ receptors may play an important role in PC12 cell differentiation, one of the characteristics of which is neurite outgrowth, induced by the hormones under lower pH. In the present study, we isolated several clones which potentiate neurite outgrowth by PACAP in acidic pH (6.8), but not in alkaline pH (7.6). RT-PCR and electrophysiology data indicate that these clones express only functional $P2X_2$ receptors in the absence or presence of PACAP for 3 days. Potentiation of neurite outgrowth resulted from PACAP (100 nM) in acidic pH is inhibited by the two P2X receptor antagonists, suramin and PPADS ($100\;{\mu}M)$ each), and exogenous exprerssion of ATP-binding mutant $P2X_2$ receptor subunit ($P2X_2[K69A]$). However, acid sensing ion channels (ASICs) are not involved in PACAP-induced neurite outgrowth potentiation in lower pH since treatments of an inhibitor of ASICs, amyloride ($10\;{\mu}M$), did not give any effects to neurite extension. The vesicular proton pump ($H^+-ATPase$) inhibitor, bafilomycin (100 nM), reduced neurite extension indicating that ATP release resulted from $P2X_2$ receptor activation in PC12 cells is needed for neurite outgrowth. These were confirmed by activation of mitogen activated protein kinases, such as ERKs and p38. These results suggest roles of ATP and $P2X_2$ receptors in hormone-induced cell differentiation or neuronal synaptogenesis in local acidic environments.