• Journal of Life Science
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• v.17 no.2 s.82
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• pp.167-173
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• 2007

To study the transcriptional mechanisms by which expression of the murine glial cell-derived neurotrophic factor inducible transcription factor (mGIF) gene is regulated, a murine genomic clone was iso-lated using a mGIF cDNA as probe. A 13-kb genomic fragment, which comprises 4-kb upstream of the transcription initiation site was sequenced. The promoter region lacks a TATA box and CAAT box, is rich in G+C content, and has multiple putative binding sites for the transcription factor Spl. The mGIF gene also has consensus sequences for AP2 binding sites. The transcriptional activity of five deletion mutants of a 2.1-kb fragment was analyzed by modulating transcription of the heterologous luciferase gene in the promoterless plasmid pGL2-Basic. All mutants showed significant transcriptional activity in the murine neuroblastoma cell line NB41A3. Transient expression assays suggested the presence of a positive regulator between -213 and -129 while a negative regulator was found in the region between -806 and -214. Relatively strong transcriptional activity was observed in neuronal NB41A3, glial C6 cells and hepatic HepG2, but very weak activity in skeletal muscle C2C12 cells. These findings confirm the tissue-specific activity of the mGIF promoter and suggest that this gene shares structural and functional similarities with the dopamine receptor genes that it regulates.

• Journal of Plant Biotechnology
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• v.43 no.2
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• pp.231-239
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• 2016

To study the transcript levels of epigenetically regulated genes in tobacco, we have developed a transgenic line OX1 overexpressing NtROS2a gene encoding cytosine DNA demethylation and a RNAi plant line RNAi13. It has been reported that salt- and $H_2O_2$-stress tolerance of these transgenic lines are enhanced with various phenotypic characters (Lee et al. 2015). In this paper, we conducted microarray analysis with Agilent Tobacco 4 x 44K oligo chip by using overexpression line OX1, RNAi plant line RNAi 13, and wild type plant WT. Differentially expressed genes (DEGs) related to metabolism, nutrient supply, and various stressed were up-regulated by approximately 1.5- to 80- fold. DEGs related to co-enzymes, metabolism, and methylation functional genes were down-regulated by approximately 0.03- to 0.7- fold. qRT-PCR analysis showed that the transcript levels of several candidate genes in OX1 and RNAi lines were significantly (p < 0.05) higher than those in WT, such as genes encoding KH domain-containing protein, MADS-box protein, and Zinc phosphodiesterase ELAC protein. On the other hand, several genes such as those encoding pentatricopeptide (PPR) repeat-containing protein, histone deacetylase HDAC3 protein, and protein kinase were decreased by approximately 0.4- to 1.0- fold. This study showed that NtROS2a gene encoding DNA glycosylase related to demethylation could regulate adaptive response of tobacco at transcriptional level.

• Journal of Microbiology and Biotechnology
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• v.30 no.9
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• pp.1412-1419
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• 2020

Human noroviruses (HuNoVs) are a leading cause of gastroenteritis outbreaks worldwide. However, the paucity of appropriate cell culture models for HuNoV replication has prevented developing effective anti-HuNoV therapies. In this study, first, the replication of the virus at various temperatures in different cells was compared, which showed that lowering the culture temperature from 37℃ significantly increased virus replication in Madin-Darby canine kidney (MDCK) cells. Second, the expression levels of autophagy-, immune-, and apoptosis-related genes at 30℃ and 37℃ were compared to explore factors affecting HuNoV replication. HuNoV cultured at 37℃ showed significantly increased autophagy-related genes (ATG5 and ATG7) and immune-related genes (IFNA, IFNB, ISG15, and NFKB) compared to mock. However, the virus cultured at 30℃ showed significantly decreased expression of autophagy-related genes (ATG5 and ATG7), but not significantly different major immune-related genes (IFNA, ISG15, and NFKB) compared to mock. Importantly, expression of the transcription factor FOXO1, which controls autophagy- and immune-related gene expression, was significantly lower at 30℃. Moreover, FOXO1 inhibition in temperature-optimized MDCK cells enhanced HuNoV replication, highlighting FOXO1 inhibition as an approach for successful virus replication. In the temperature-optimized cells, various HuNoV genotypes were successfully replicated, with GI.8 showing the highest replication levels followed by GII.1, GII.3, and GII.4. Furthermore, ultrastructural analysis of the infected cells revealed functional HuNoV replication at low temperature, with increased cellular apoptosis and decreased autophagic vacuoles. In conclusion, temperature-optimized MDCK cells can be used as a convenient culture model for HuNoV replication by inhibiting FOXO1 and providing adaptability to different genotypes.

• The Transactions of the Korea Information Processing Society
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• v.5 no.8
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• pp.1960-1971
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• 1998

The researches and developments to provide multimedia communication services such as Video on Demand(VoDJ), real time video phonc and multipoint vidco conferencing on broadband ISDN environmcnts have been proceeded with activity. Specifications for Vol) services which is worked by Digital Audio-Visual Council(DAVIC) to support detail technologies including total service system that is consist of VoD server. delive[\! networl, and Set-Top Box(STB) had been already finished and ITU-T SG16 also recommended the standards of H.300 series terminal aspects for conversational multimedia services, But the architectures of multimedia tenninals recommended and specified by these organizations do not have an efficient st11lcture to provide all of retrieval, distrihution and conversational service due to a different point of view about multimedia terminals and services. In this paper, we analyzed the recornmendatio!E and the specifications of intemational public and private organizations like lTU-T, DAVIC and ATM forum. As a result of these analysis. we propose an efficient terminal architecture, and then we have designed, lmplemented the multimedia communication terminal for offering VoI) and real- time conversation ,,, functional module test according to the individual commumication service session and confirined the validiry or terminal implemented to be used on broadband ISDK environments.

• Journal of Music and Human Behavior
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• v.19 no.2
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• pp.81-103
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• 2022

The effects of therapeutic instrumental music performance (TIMP) using a ukulele were examined in adults with central nervous system damage and impaired hand functions. The participants were three adults with neurological damage who participated in 30-min sessions twice a week over 6 weeks. Changes in hand function was measured by the Box and Block Test (BBT), the 9-Hole Peg Test (9-HPT), and the Jebsen-Taylor Hand Function Test (JTHFT). Following the intervention, all three participants showed increases in the BBT and 9-HPT scores, indicating positive changes in fine motor coordination and dexterity. In terms of the JTHFT, all three participants showed increases in the "writing" and "card flipping" subtask scores, indicating that the intervention was effective in improving more coordinated finger movements. All participants reported the satisfaction with the intervention. They also pointed out that they were motivated to play the ukulele and that following the intervention used their affected hand more frequently in daily activities. These findings suggest that TIMP with a ukulele for patients with central nervous system damage can have positive effects on their functional hand movements and motivate these patients to practice their rehabilitation exercises.

• Food Science and Preservation
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• v.30 no.1
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• pp.122-131
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• 2023

Persimmon peels are mostly discared as a by-product of dried persimmon manufacturing, but effective use is required as it contains various functional constituents. This study covers the preparation of carotenoid-enriched oil by ultrasound treatment of persimmon peel in soybean oil solvent, and the investigation of its physicochemical characteristics. Using the Box-Behnken design and response surface methodology, the optimal extraction conditions (temperature, 62℃; time, 32 min; and persimmon peel to oil ratio, 1:3.1) were determined based on the carotenoid concentration of the extract. The physicochemical characteristics of the extract obtained under optimal conditions and the untreated soybean oil (control) were compared. The total carotenoid content of the extract increased. The carotenoid-enriched soybean oils had a lighter color than the controls, but with high redness and yellowness values. The effect of sonication and a component of the persimmon peel on the oxidation and heating stability of soybean oil was weak. The viscosity and activation energy of carotenoid-enriched soybean oil were slightly higher than those of the control. Thus, it was possible to prepare yellow-red carotenoid-enriched soybean oil by applying ultrasonic-soybean oil solvent extraction to persimmon peel. The oil is expected to be useful as an additive as well as a substitute for general edible oils.

• Journal of the Korean Geosynthetics Society
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• v.18 no.4
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• pp.215-224
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• 2019

According to the recent civil engineering construction work site which is a complex process, development of multi-functional geotextiles is required. In this study, the characteristics of five different modified cross-section fiber yarns for the selection of wicking yarns were analyzed and yarns that can achieve target properties were selected. Experimental prototype geotextiles suitable for horizontal wicking drain property and reinforcement was developed and its tensile strength, 2% secant modulus, vertical water permeability, AOS, friction characteristics by the direct shear method, and vertical/horizontal wicking test were analyzed. These tests are conducted to verify the performance of the geotextiles with horizontal wick drain property, separation and reinforcement developed in this study. As a results of the indoor soil box test, it was confirmed that the geotextiles using the wicking yarn sufficiently exhibited the function of discharging excess pore water in the horizontal direction.

• The Journal of Korean Institute of Electromagnetic Engineering and Science
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• v.12 no.2
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• pp.184-192
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• 2001

In this paper, we analyze the result of the conduced emission and susceptibility tests performed on the KOMPSAT-I ETB Platform. The ETB platform consists of the EM (Engineering Model) boxes developed to perform the electrical functional test. During the conducted emission test, we measured the instant waveform in time domain when each switch was turn on, and spectrum of the noise in the frequency range of 10 Hz up to100 MHz fur the steady state of the ETB. During the conducted susceptibility test, no malfunction and no serious damage of the electronic box were observed when a simulated noise waveform was applied to the DC power bus. The simulated waveform was obtained by adding the 6 dB-system margin to the worst case waveform measured from the conducted emission. This test was performed as co-development of the KOMPSAT-I with TRW in USA.

• Journal of the Korean Society of Physical Medicine
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• v.9 no.4
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• pp.485-492
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• 2014

PURPOSE: This study aimed to examine the pain, range of motion (ROM), upper extremity task performance, and functional levels of patients after rotator cuff repair according to the timing of a closed chain exercise thereby presenting basic data for an effective rehabilitation program. METHODS: The intervention was applied three times per week, one hour per day, for four weeks to 40 participants, 78 of whom had undergone rotator cuff repair. The participants were divided into four groups and assigned to usual general physical therapy and an open chain exercise. Group I consisted of the open chain exercise only. The closed chain exercise was applied to group II after the 4 times, group III after the 7 times, group IV after the 10 times. Measurement were used ROM, visual analogue scale (VAS), box and block test (BBT), and shoulder pain and disability index (SPADI). A one-way analysis of variance was conducted to test differences. RESULTS: There were significant differences in the internal/external rotation between group I and group II. The VAS significantly differed between group II and group I, group III, and group IV. The BBT results of group II and group I were significantly different compared to those of group IV. The SPADI significantly differed between group II and group I and between group II and group IV. CONCLUSION: The closed chain exercise was effective for patients following rotator cuff repair from the second week after active exercise was prescribed, verifying its applicability in rehabilitation programs.

• 한국HCI학회:학술대회논문집
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• 2009.02a
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• pp.895-900
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• 2009

This study focused on user centered design methodology on mobile service design, Especially we designed business supporting service for sale persons with heavy mobile uses in their works. We used contextual inquiry interview to understand user's work context and mobile usage. The findings are applied to the service concept, And information architecture, interaction design, GUI design is followed. As a result of design research, Post widget service is designed for instant information organization and functional relationship. And Memory box widget service is designed for business network management and contextual findability. Those services are developed for widget design on touch based phone platform.