• Title/Summary/Keyword: fuji apple

Search Result 253, Processing Time 0.025 seconds

Cloning and Phylogenetic Characterization of Coat Protein Genes of Two Isolates of Apple mosaic virus from ¡?Fuji¡? Apple

  • Lee, Gung-Pyo;Ryu, Ki-Hyun;Kim, Hyun-Ran;Kim, Chung-Sun;Lee, Dong-Woo;Kim, Jeong-Soo;Park, Min-Hye;Noh, Young-Mi;Choi, Sun-Hee;Han, Dong-Hyun;Lee, Chang-Hoo
    • The Plant Pathology Journal
    • /
    • v.18 no.5
    • /
    • pp.259-265
    • /
    • 2002
  • Apple mosaic virus (ApMV), a member of the genus Ilarvirus, was detected and isolated from diseased 'Fuji' apple (Malus domestica) in Korea. The coat protein (CP) genes of two ApMV strains, denoted as ApMV-Kl and ApMV-K2, were amplified by using the reverse transcription and polymerase chain reaction (RT-PCR) and were analyzed thereafter. The objectives were to define the molecular variability of genomic information of ApMV found in Korea and to develop virus-derived resistant gene source for making virus-resistant trans-genic apple. RT-PCR amplicons for the APMVS were cloned and their nucleotide sequences were determined. The CPs of ApMV-Kl and ApMV-K2 consisted of 222 and 232 amino acid residues, respectively. The identities of the CPs of the two Korean APMVS were 93.1% and 85.6% at the nucleotide and amino acid sequences, respectively. The CP of ApMV-Kl showed 46.1-100% and 43.2-100% identities to eight different ApMV strains at the nucleotide and amino acid levels, respectively. When ApMV-PV32 strain was not included in the analysis, ApMV strains shared over 83.0% and 78.6% homologies at the nucleotide and amino acid levels, respectively. ApMV strains showed heterogeneity in CP size and sequence variability. Most of the amino acid residue differences were located at the N-termini of the strains of ApMV, whereas, the middle regions and C-termini were remarkably conserved. The APMVS were 17.(1-54.5% identical with three other species of the genus Ilarviyus. ApMV strains can be classified into three subgroups (subgroups I, II, and III) based on the phylogenetic analysis of CP gene in both nucleotide and amino acid levels. Interestingly, all the strains of subgroup I were isolated from apple plants, while the strains of subgroups II and III were originated from peach, hop, or pear, The results suggest that ApMV strains co-evolved with their host plants, which may have resulted in the CP heterogeneity.

Growth Control of Upper Part in 'Fuji'/M.9 Apple Tree Canopy by Cutting Time of Trunk and Plant Growth Regulators (주간 절단시기 및 생장조절제를 이용한 '후지'/M9 사과나무 수관 상단부 생장조절)

  • Sagong, Dong-Hoon;Lee, Jae-Wang;Yoon, Tae-Myung
    • Korean Journal of Environmental Agriculture
    • /
    • v.37 no.2
    • /
    • pp.87-96
    • /
    • 2018
  • BACKGROUND: The vigorous shoot growth in upper part of apple tree canopy leads to poor fruit quality and flower bud formation in lower part of canopy. So, this study was conducted to develop the proper control method about the shoot growth in upper part of apple tree canopy. METHODS AND RESULTS: Trunks of 'Fuji'/M9 apple trees were cut (back pruned) to 2.5 m in tree height on 11 February (dormant) or 12 April (full bloom). Naphthalene acetic acid (NAA) was applied at 2.0% to cut surface when trunk was pruned. Prohexadione-calcium (Pro-Ca) was sprayed at 250 mg/L above 2.0 m in tree height at 23 April (petal fall). The NAA or Pro-Ca application after trunk was pruned at dormant (TR-2 and TR-3) significantly reduced shoot growth in upper part of canopy compared with the control (tree was only pruned at dormant, TR-1), but the percent of shoots showing the secondary growth of TR-3 was higher over 2 times than that of TR-2. The reduction of shoot growth in upper part of canopy by TR-2 and TR-3 increased the fruit red color from the lower part in the treating year and blooming of the lower part in the following year. CONCLUSION: Applying 2.0% NAA to cut surface of pruned apple trunk at dormant was the most effective way for stabilization of the tree vigor in upper part of the canopy in a high density apple orchard.

Application of Near Infrared Reflectance Spectroscopy as a Rapid Leaf Analysis Method to Evaluate Nutritional Diagnosis in Apple (Malus Domestica Borkh, Fuji) and grape(Vitis Labrusca, Campbell Early) (영양진단을 위한 신속한 엽분석 방법으로서 근적외분광분석기의 이용)

  • Seo, Young-Jin;Park, Man;Kim, Chang-Bae;Kim, Jong-Su;Yoon, Jae-Tak;Cho, Rae-Kwang
    • Korean Journal of Soil Science and Fertilizer
    • /
    • v.33 no.4
    • /
    • pp.242-246
    • /
    • 2000
  • The Near Infrared Reflectance Spectroscopy(NIR) was used to evaluate nutritional diagnosis for rapid leaf analysis method, 177 'Fuji' apple and 130 'Campbell Early' grape leaves were measured by Near Infrared reflectance spectra in the NIR region(1,100~2.500nm). Total nitrogen content was measured by kjelldhal distillation, after salycilic acid-sulfuric acid digestion. An empirical equation to predict total nitrogen content from its spectral signature was developed by adapting the Near Infrared Reflectance Spectroscopy analysis(NIRa) technique and the results were apple-0.965(R). 0.086(SEC), grape-0.926(R), 0.152(SEC). Standard Error of Prediction(SEP) of NIRa for predicting the total nitrogen of apple and grape leaves was 0.360 and 0.210, respectively. It was concluded that Near infrared reflectance spectroscopy analysis is promising method for rapid analysis of apple and grape leaves.

  • PDF

Determination on the Optimal Harvest Date of Apples for CA Storage (CA 저장을 위한 사과의 최적 수확시기 결정)

  • 정헌식;정신교
    • Food Science and Preservation
    • /
    • v.1 no.1
    • /
    • pp.29-36
    • /
    • 1994
  • This study was carried out for the establishment of optic harvest date and storage condition of 'Fuji' apple for CA storage. Apples were picked at 10 day intervals from 17 Sept to 27 Oct.. Apples were analyzed for respiration rata, weight flesh firmness, titratable acidity, soluble solid, total sugar, reducing sugar, surface color and thiault value. In 1990, the optimal harvest season could be judged between 17 Oct. and 27 Oct. from the results of respiration rate rise and change of quality properties. Apples harvested on 27 Oct. wert stored at 2$^{\circ}C$ and 90~95% RH in 1%O2 + 3%CO2, 3%O2 + 3%CO2 and air for 9 months. After 9 months of storage, apples kept in CA storages had beater quality than apples kept in refrigeration storage. Especially, apples kept in 1%O2, + 3%CO2 were fumer and more acid than those kept in 3%O2 + 3%CO2 At the sensory evaluations, apples stored in CA were rated higher quality than apples stared refrigeration.

  • PDF

First Report of Two Colletotrichum Species Associated with Bitter Rot on Apple Fruit in Korea - C. fructicola and C. siamense

  • Park, Myung Soo;Kim, Byung-Ryun;Park, In-Hee;Hahm, Soo-Sang
    • Mycobiology
    • /
    • v.46 no.2
    • /
    • pp.154-158
    • /
    • 2018
  • Bitter rot caused by the fungal genus Colletotrichum is a well-known, common disease of apple and causes significant yield loss. In 2013, six fungal strains were isolated from Fuji apple fruits exhibiting symptoms of bitter rot from Andong, Korea. These strains were identified as Colletotrichum fructicola and C. siamense based on morphological characteristics and multilocus sequence analysis of the internal transcribed spacer rDNA, actin, calmodulin, chitin synthase, and glyceraldehyde-3-phosphate dehydrogenase Pathogenicity tests confirmed the involvement of C. fructicola and C. siamense in the development of disease symptoms on apple fruits. This is the first report of C. fructicola and C. siamense causing bitter rot on apple fruit in Korea.

First Report of Fruit Rot Caused by Fusarium decemcellulare in Apples in Korea

  • Lee, Seung-Yeol;Park, Su-Jin;Lee, Jae-Jin;Back, Chang-Gi;Ten, Leonid N.;Kang, In-Kyu;Jung, Hee-Young
    • The Korean Journal of Mycology
    • /
    • v.45 no.1
    • /
    • pp.54-62
    • /
    • 2017
  • In 2014, abnormal brown spots were observed on Hongro apples in fields in Gyeongsangbuk-do Province and during low-temperature storage. The spots were round, blight brown, and different from the symptoms of previously reported apple diseases. A fungal pathogen was isolated and cultured on potato dextrose agar, and it was morphologically similar to Fusarium decemcellulare. A pathogenicity test showed the same brown spots on both wounded and unwounded Hongro and Fuji apple cultivars. RPB1 and RPB2 sequences of F. decemcellulare KNU-GC01 matched with those of F. decemcellulare NRRL 13412 (98.3% and 97.6% similarities, respectively); both strains clustered together in the phylogenetic tree, indicating their close relationship at the species level. Therefore, F. decemcellulare is a newly reported pathogen that causes brown spots on apples in Korea.

Unreported Post-harvest Disease of Apples Caused by Plenodomus collinsoniae in Korea

  • Das, Kallol;Kim, Yeong-Hwan;Yoo, Jingi;Ten, Leonid N.;Kang, Sang-Jae;Kang, In-Kyu;Lee, Seung-Yeol;Jung, Hee-Young
    • The Korean Journal of Mycology
    • /
    • v.48 no.4
    • /
    • pp.511-518
    • /
    • 2020
  • This study was conducted to isolate and identify the fungal pathogen caused unreported post-harvest disease on apples (cv. Fuji) fruit in Korea. The disease symptoms on apples appeared as irregular, light to dark brown, slightly sunken spots. The three fungal strains were isolated from infected tissues of apple fruits and their cultural and morphological characteristics were completely consistent with those of Plenodomus collinsoniae. The phylogenetic analysis using the internal transcribed spacer (ITS) regions, beta-tubulin (TUB), and the second largest subunit of RNA polymerase II (RPB2) sequences revealed the closest relationship of the isolates with Plenodomus collinsoniae at the species level. The pathogenicity test showed the same dark brown spots on Fuji apple cultivar. Therefore, P. collinsoniae is a newly reported fungal agent causing post-harvest disease on apples in Korea.

Effects of Tree-spray of Calcium Formate Compound Extracted from Oyster Shell and Active Agent on the Calcium Concentration, Fruit Skin Shape, and Quality of 'Fuji' Apple Fruit (굴 껍데기에서 개미산으로 추출한 칼슘화합물과 활성제의 수관살포가 사과 '후지' 과실의 칼슘농도, 과피 형태 및 품질에 미치는 영향)

  • Moon, Byung-Woo;Kang, In-Kyu
    • Journal of Bio-Environment Control
    • /
    • v.16 no.1
    • /
    • pp.21-26
    • /
    • 2007
  • This study was carried out to investigate the effects of tree-spray of calcium formate compound extracted from oyster shell with several active agents on the calcium concentration, fruit skin shape and quality of 'Fuji' apple. The tree-spray of calcium formate compound extracted from oyster shell (Os-CaF, $52.4\;mg{\cdot}kg^{-1}$) appeared to have more effectiveness on the calcium translocation into leaves, fruit skin and flesh than control. Addition of adjuvants to Os-CaF increased calcium concentration of 'Fuji' apple when sprayed three times before harvest. Among the active agents examined, the treatment ascorbic acid and poly-vinyl alcohol (PVA) in leaves, Ag-colloidal and PVA in fruit skin, ascorbic acid, Ag-colloidal, and PVA in fruit flesh exhibited highest effectiveness. Fruit qualities (fruit weight, firmness, soluble solids, acidity and Hunter value) were not affect by Os-CaF on different concentrations and solutions of active agents.

Freshness of 'Fuji' Apples Packed Anti-fogging Agents Film (결로 방지 필름으로 포장한 '후지' 사과의 신선도)

  • Park, Hyung-Woo;Ryu, Na-Hee
    • KOREAN JOURNAL OF PACKAGING SCIENCE & TECHNOLOGY
    • /
    • v.19 no.1
    • /
    • pp.29-33
    • /
    • 2013
  • This study investigated the effect of films treated with anti-fogging (LA) agent on the freshness extension of 'Fuji' Apples. Preference, weight loss, total ascorbic acid, sugar content, acidity, change of gas composition in package were evaluated during storage at $15^{\circ}C$. After 150 days of storage, the weight loss of apples in control (L), LA was 1.0 to 1.1%. Total ascorbic acid content of apples in control after 150 days was 2.09 mg/100g F.W, that of apple in LA was 2.47 mg/100g F.W. The titratable acidity of apple in LA was lower than that in control, while soluble solids content of LA was higher than that in control after 150 days. Ethylene gas adsorbability in control package was 192.2 ppm and that in LA was 195.7 ppm. It was verified that LA treated with anti-fogging agent are few different compare to control, but commerdity on the display in market was considered higher than that of non-treated anti-fogging agent.

  • PDF