• Korean Journal of Food Science and Technology
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• v.23 no.4
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• pp.442-446
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• 1991

Three peroxidase fractions (peak I, II, III) were isolated from Fuji apples using CM-cellulose chromatography. The homogeneity of the isolated peroxidase isozymes was established by isoelectric focusing and electrophoresis. Isoelectric points of the isozymes were 3.80, 3.82, and 3.85, respectively. The optimum pH of peroxidase isozymes were pH 5.0(peak I) or 5.5(peak II, III), and optimum temperature was $40^{\circ}C$ when assayed by using guaiacol and $H_{2}O_{2}$ as substrates. Inactivation rate of three peroxidase isozymes were different at temperature of $70^{\circ}C$ and at pH of 5.5. The isozyme of peak II was found to be more heat stable than those of peak I and III. D values at $70^{\circ}C$ of peroxidase isozymes (peak I, II, III) were estimated to be 660 sec, 1,320 sec, and 600 sec, respectively. The thermal stability of Fuji apple peroxidase was not influenced in the presence of 0.032 M sucrose or lactose. However, the thermal stability of the enzyme was decreased by fructose and glucose.

• Food Science and Preservation
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• v.14 no.1
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• pp.42-46
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• 2007

To investigate the effects of hot water treatment and modified atmosphere packaging (MAP), compared with non-packaging, of 'Fuji' apples during 18 weeks of storage at $0^{\circ}C$, apple weight loss, firmness, titratable acidity, total ascorbic acid and sensory characteristics were measured After 18 week of storage, the weight loss of MAP-treated apples was 1%, while untreated controls lost 22% of weight Weight loss reduction film packaging was more effective than that afforded by hot water treatment. The firmness reductions in control apples, those receiving hot water treatment those receiving packaging only, and those receiving both hot water treatment and packaging, were 37%, 22%, 10% and 6%, respectively. The titratable acidity was 40% in control apples and respectively, 37%, 32% and 27% in the three groups mentioned above. The total ascorbic acid contentuntreated control apples decreased by 70% after 18 weeks of storage. The total ascorbic acid contentof apples receiving both hot water use of both packagingand hot water treatment to preserve 'Fuji' apple quality.

• Horticultural Science & Technology
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• v.30 no.5
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• pp.527-533
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• 2012

This study was carried out to investigate the effects of aminoethoxyvinylglycine (AVG) application and heat treatment on fruit quality of 'Fuji' apples during CA storage. AVG ($75mg{\cdot}L^{-1}$) was applied to 'Fuji' tree at 150 and 175 days after full bloom (DAFB) and the fruit were harvested at 185, 195, and 205 DAFB, respectively. Respiration rate and ethylene production of fruit applied with AVG were greatly reduced more than those in control. Flesh firmness and acidity of fruit applied with AVG were higher than those in control. Flesh browning occurred in all of the control fruit regardless of harvest date. However, AVG-treated fruits were free of flesh browning except for late harvested fruits. At 185 DAFB, 'Fuji' apples were harvested and prestorage heat treatment was done for 3 days at $38^{\circ}C$ and 6 hours at $46^{\circ}C$. Heat treatment at $38^{\circ}C$ and $46^{\circ}C$ prior to CA storage greatly reduced respiration rate and ethylene production. The incidence of flesh browning were 35%, 14%, and 5%, in control fruit, heating at $38^{\circ}C$ and $46^{\circ}C$, respectively. The titratable acidity was lower at heated apple than at controlled one.

• The Journal of Natural Sciences
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• v.6 no.1
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• pp.49-54
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• 1993

This study was conducted to investigate the influence of various calcium salts on the accumulation of calcium in apple fruits when sprayed on whole tree. Differences in the total calcium contents of fruits were found between calcium sources and cultivars. In 'Tsugaru' , calcium nitrate, calcium chloride, and calcium carbonate were all effective but only calcium chloride and calcium carbonate appeared to be effective in 'Fuji' . Major parts of the applied calcium were accumulated in the feel and outer flesh. Ethylene evolution of fruit was retarded during storage with the increase of total calcium content in 'Fuji' fruit treated calcium acetate.

• Korean Journal of Breeding Science
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• v.42 no.5
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• pp.525-533
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• 2010

In this study, random amplified polymorphic DNA (RAPD) and simple sequence repeat (SSR) analyses were utilized for evaluation of genetic diversity of 34 Korean bred and introduced apple cultivars. Thirty-seven RAPD primers detected a total of 193 polymorphic bands (36.2%) with an average of 5.6. Twenty-six SSR markers generated a total of 112 alleles with an average 4.3 alleles per locus. Genetic diversity of 34 cultivars estimated by polymorphic information content (PIC) value ranged from 0.536 (CH03d12) to 0.952 (CH04c06) with an average of 0.843. By UPGMA (unweighted pair-group method arithmetic average) cluster analysis with 305 polymorphic bands, the apple cultivars were classified four groups by similarity index of 0.640. The 'Seokwang' was included in group I. Group II consisted of 12 cultivars which have 'Golden Delicious' in their pedigree, with the exception of 'Spur Earliblaze' and 'Jonathan'. Group III included 13 cultivars which have usually 'Fuji' in their ancestry and bud sport of 'Fuji' cultivars. Group IV consisted of 8 cultivars with 'Hongro', 'Gamhong', and 'Saenara'. Similarity values among the tested apple cultivars ranged from 0.529 to 0.987, and the average similarity value was 0.647. The similarity index was the highest (0.987) between 'Hwarang' and 'Danhong', and the lowest (0.529) between 'Seokwang' and 'Hwarang'. The genetic relationships among the 34 studied apple cultivars were basically consistent with the known pedigree.

• Proceedings of the Korean Society of Near Infrared Spectroscopy Conference
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• 2001.06a
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• pp.1529-1529
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• 2001

The watercore in apple is very important factor in storage and sorting of fruit. Most consumers tend to prefer the apple included watercore in immediately after harvest, however the watercore causes fruit flesh to brown during storage and lose the worth after all. But it is practically impossible to judge to the naked eye whether an apple has watercore or not. Therefore, the rapid, accurate and non-destructive analysis method for discrimination of watercore should be settled without delay. In this study we attempted the discrimination and quantitative analysis of watercore in apple fruit using near-infrared transmittance spectroscopy ‘Fuji’ apple fruits produced in Kyungpook of Korea was used in this experiment. The watercore content in apple was evaluated by graphic treatment of culled slice sections(10 mm). NIR transmittance spectra were collected over the 500 to 1000 nm spectral region with a spectrometer (Sentronic Co., Germany). The calibration models were carried out by partial least squares (PLS) analysis between NIR spectra data of apples and chemical data of watercore content. The spectra were different in absorbance between apple included watercore and not included one. Apple included watercore had higher absorption band than sample not included one at 732 and 820 nm. The calibration model seems to be accurate to predict the watercore content in apple fruit, the correlation coefficient (R) and root mean square error of prediction (RMSEP) were 0.99 and 0.93%, respectively. This result indicates that the PLSR calibration model by using NIR transmittance spectroscopy could be used for discrimination of watercore in apple fruit.

• Journal of Plant Biotechnology
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• v.42 no.3
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• pp.271-276
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• 2015

Crab apple cultivars, 'Maypole', 'Tuscan', and 'Manchurian', were evaluated as potential pollinizers of major apple cultivars, 'Fuji', 'Hongro', and 'Tsugaru', cultivated in high-density apple orchard systems. Numerous cultivar characteristics, including blooming time, pollen germination, fruit set, disease and pest resistance, and self-incompatibility, were examined. The blooming times of both 'Maypole' and 'Tuscan' ranged from April 19 to May 5, which was 2-4 days earlier than those of the major commercial apple cultivars. PCR analysis did not reveal the presence of any of the S-alleles (S1, S3, S7, or S9) identified in major commercial apple cultivars. In addition, the percentage of the fruit set was high after trees were artificially fertilized with crab apple pollen. Artificially cross-pollinated fruits were of similar or higher quality than open-pollinated fruits. They also demonstrated resistance to apple blotch, sooty blotch, and fly speck. The results indicate that the two crab apples, 'Maypole' and 'Tuscan' would be potential candidates for pollinizers of major apple cultivars in Korea. Use of the pollen of these crap apples in commercial production will improve fruit quality and promote sustainable and robust fruit production.

• Research in Plant Disease
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• v.24 no.4
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• pp.249-256
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• 2018

Disease occurrence in apple cultivars on 13-30 apple orchards from April to October 1992-2010 was investigated at 30 days intervals in Gyeongbuk, Gyeongnam and Jeonbuk provinces of Korea. Apple cultivar 'Fuji' was found most susceptible and is being damaged by cedar apple rust, scab, gray mold, Alternaria blotch and Marssonia blotch as major leaf diseases that were observed. The percentage of occurrence of these leaf diseases varies from every year. This variation is based on different rainfall and temperature conditions, cultivation, root stock and changed system of fungicide spray during the particular year and season.

• Korean Journal of Soil Science and Fertilizer
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• v.34 no.6
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• pp.380-386
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• 2001

This study was conducted to investigate the influence of the root zone temperature on the changes in soil physicochemical environment and the growth of the apple trees. Root zone temperatures applied were 20. 25. 30. and $35^{\circ}C$. A pot experiment of 4-year-old fruit-bearing Fuji/M.26 apple trees was done from May 15. 1997 to August 15. 1997. Shoot length and shoot number of 4-year-old Fuji/M.26 was longest at $30^{\circ}C$ but decreased at $35^{\circ}C$. Oxygen concentrations in soil air were maintained from 17 to 18% at $20^{\circ}C$ but fluctuated from 10 to 14% at the end at $35^{\circ}C$. Carbon dioxide concentrations in soil air was highest at $35^{\circ}C$. Organic matter contents in soil decreased as root zone temperature and duration of treatment increased. Nitrate and ammonium contents in soil increased with rising root zone temperature in the beginning but nitrate contents decreased at $35^{\circ}C$ as time progressed. Nitrate content was higher in 10~15cm subsoil than in 25~30cm subsoil.

• KOREAN JOURNAL OF PACKAGING SCIENCE & TECHNOLOGY
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• v.19 no.2
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• pp.87-94
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• 2013

To investigate the effects of functional MA films (FMA) masterbatched by zeolite powder treated with 1 N HCl, 0.5 N NaCl solution and anti-fogging agent (NA) compared with control on the freshness extension of 'Fuji' apples during storage at $15^{\circ}C$. Preference, weight loss, total ascorbic acid, sugar content, acidity, change of gas composition in package were measured. After 150 days of storage, the weight loss of control (L) apples was 1%, that of apple in FMA film (NA) was 1.5% after 150 days. Total ascorbic acid content of apples in control (L) after 150 days was 2.09 mg%, those of apple in FMA film (NA) was 2.72 mg%. The titratable acidity of apple in FMA film was higher than that in control, while soluble solids content of apples in FMA film was lower than that in control after 150 days. Ethylene gas adsorbability in control was 192.2 ppm and those in FMA film was 141.4 ppm after 150 days. Overall, apples in FMA film was better than that of control. It was verified that apples packed with LLDPE film (control) lasted about 130days, while those with FMA films lasted about 170 days. Also, FMA films treated with anti-fogging agent few different compare to nontreated film, but commerdity on the display in market was considered higher than that of non-treated anti-fogging agent.