• Title/Summary/Keyword: frutose-6-phosphate phospoketolase

Search Result 1, Processing Time 0.016 seconds

Development of Bifidobacterium bifidum Strains Resistant to Rifampicin (리팜피신에 내성인 Bifidobacterium bifidum 균주개발)

  • 최응칠;고성열;김희선;최성숙;김숙경;김병각
    • YAKHAK HOEJI
    • /
    • v.37 no.5
    • /
    • pp.483-489
    • /
    • 1993
  • Bifidobacterium bifidum, one strain of medical preparations being on the market for human intestinal disorder, is very sensitive to rifampicin. If this preparation is taken with rifampicin, its therapeutic effect can't be expected. To develope rifampicin resistant mutants, B. bifidum was treated with N-methyl-N'-nitro-N-nitrosoguanidine(MNNG). All of thirty strains grown on the plates containing 10 $\mu\textrm{g}$/ml rifampicin were over 1, 000 times more resistant to rifampicin than parental strain and they were identified as B. bifidum by fructose-6-phosphoate phosphoketolase test. Three strains out of thirty, which produced almost same amount of organic acid as parental strain, were selected for further studies. They showed identical growth inhibition activity aganist E. coli compared with that of parental strain. And rifampicin was not inactivated.

  • PDF