• Title/Summary/Keyword: fruit and seed

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Characterization of Phenotypic Traits and Application of Fruit Flesh Color Marker in Melon (Cucumis melo L.) Accessions (멜론 유전자원의 생육 평가와 과육색 유전형 분석)

  • Bae, Ik Hyun;Kang, Han Sol;Jeong, Woo Jin;Ryu, Jae Hwang;Lee, Oh Hum;Chung, Hee
    • Korean Journal of Plant Resources
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    • v.34 no.5
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    • pp.478-490
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    • 2021
  • We aimed to generate basic breeding data for melon (Cucumis melo L.). A total of 219 melon accessions conserved at the National Agrobiodiversity Center (NAC) in Rural Development Administration (RDA) were used in this study, of which 72 (33%) were collected from India. The majority of accessions showed orange (42%) and white (36%) flesh color. In addition to phenotypic evaluations, the accessions were genotyped using a molecular marker for the carotenoid biosynthesis gene CmOr. DNA fragments of the expected size were amplified in 205 out of 219 accessions. Digestion of the PCR products with HinfI restriction endonuclease showed 100% concordance between phenotype and genotype in green-fleshed accessions, but 98%, 97%, and 80% concordance in orange-, white-, and creamy-fleshed accessions, respectively. Sequence analysis revealed single nucleotide changes in the three positions of SNP1, SNP2 and SNP1int in the CmOr gene among accessions. These newly found alleles suggest that there are multiple mechanisms in determining fruit flesh color in melon. Also, the phenotype data of diverse accessions obtained in this study will be a valuable source for melon breeding.

β-Carotene, Cucumisin Content and Fruit Morphology of Melon (Cucumis melo L.) Germplasm Collections

  • Kim, Yeong-Jee;Hwang, Ae-Jin;Noh, Jae-Jong;Wang, Xiaohan;Lee, Jae-Eun;Yoo, Eunae;Lee, Sookyeong;Hwang, Sojeong;Kim, Myung-Kon;Noh, Hyungjun
    • Korean Journal of Plant Resources
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    • v.34 no.6
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    • pp.555-565
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    • 2021
  • Melon fruits are a year-old plant in the family Cucurbitae and are the most cultivated fruit in tropical countries. Melon flesh is an important source of anti-cancer, antioxidant effects, attracting attention as a functional food. We investigated the morphological properties, β-carotene content, and cucumisin activity of seeds of 58 melon genetic resources. Melon resources have shown various morphological properties. Melons grouped by morphological properties were the five groups. β-carotene content varied between 82.34 mg/kg, 86.75 mg/kg, 25.56 mg/kg, 86.25 mg/kg, and 54.65 mg/kg. Between β-carotene, cucumisin activity and other quantitative fruit morphological properties, the color of the pulp and the firmness of the pulp had a significant amount of correlation between the β-carotene content. However, cucumisin activity and β-carotene content had significant negative correlation, and the color of the fruit and shape of the fruit had significant negative correlation with β-carotene content and activity of cucumisin. Our study vindicated that high diversity in melon morphological characters of genetic sources that provide beneficial baseline data for the future and in the activity of β-carotene and cucumisin, and these results could predict indicators of β-carotene content by the length of leaf, the width of fruit, the length of joint, and the length of seed, and collectively the morphological properties of melons could help predict indicators of β-carotene content and help develop functional sarcoma and farmhouse cultivation.

Seasonal Changes in Sexual Allocation within Flowers of Chelidonium majus ( Papaveraceae ) (애기똥풀 ( 양귀비과 ) 꽃에서 일어나는 성적자원 분배의 계절적 변화)

  • Kang, Hye-Son;Rihard B. Primark;Nam-Kee Chang
    • The Korean Journal of Ecology
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    • v.14 no.4
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    • pp.415-433
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    • 1991
  • Seasonal variation in size and number of floral structures was examined in two massachusetts populations of chelidonium major, a self-compatible herb. All floral charcters except for anther number per flower declind significantly during a 3 week period. However, temporal patterns were not identical among characters or between popolations. The result indicate that floral characters varied in conjunction with flower diameter,but that the pattern of changes in floral characters in response to environments may not be easy to predict. Principal components analysis was conducted to environments may not be easy to predict. principal compenents analysis was conducted tl identify the functional relationship among floral male function, and female function, respectively, perhaps reflecting the functional distinction of floral characters. Based on this pattern, the relative allocation to sexual structures within flowers was examined: male allocation was relatively greater than female allocation eary or in the middle of flowering season, depending upon populations. Temporalvariation in relative allocation within flowers was not independent of seed tield components:; different combinations of the size and number of floral characters were correlated with different seed yield components, for example, either seed size or number per fruit, during a season. in particular, allocations to attractive and male structures were highly correlated with mean seed weight only earlier in the season. These result provide some evidence that flowering phenolgy is an important comportant to be considered in the study of sexual allocation.

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Screening of Crude Drugs for Antioxidative Activity (생약추출물의 항산화 활성검색)

  • Na, Min-Kyun;An, RenBo;Lee, Sang-Myung;Hong, Nam-Doo;Yoo, Jae-Kuk;Lee, Chan-Bok;Kim, Jin-Pyo;Bae, Ki-Hwan
    • Korean Journal of Pharmacognosy
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    • v.32 no.2 s.125
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    • pp.108-115
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    • 2001
  • Based on DPPH radical scavenging activity and lipid peroxidation inhibitory activity, the MeOH extracts of 139 crude drugs were screened in order to search for antioxidants. Among tested samples, the extracts from the seed of Nelumbo nucifera, the fruit of Terminalia chebula var. gangetia, the root of Salvia miltiorhiza, the fruit of Ziziphus jujuba var. innermis, the root bark of Paeonia moutan, the fruit of Rubus coreanus, the fruit of Zanthoxylum schinifolium, the lignum of Caesalpinia sappan, the leaf of Pinus densiflora, the rhizome of Alpinia officinarum, the fruit of Syzygium aromaticum, the ramulus and uncus of Uncaria rhynchophylla, the root bark of Lycium chinense, and the fruit of Alpinia katsumadai showed a relatively strong antioxidative activity. Furthermore, the BuOH fraction from the extract of N. nucifera showed a potent activity in each assay. The isolation of bioactive compounds has been carried out and will be reported in the next paper.

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Effects of Elevated $CO_2$ Concentration and Increased Temperature on the Change of the Phenological and Reproductive characteristics of Phytolocca insularis, a Korea endemic plant ($CO_2$농도 및 온도 증가가 한국특산식물 섬자리공의 식물계절학 및 번식생태학적 특성 변화에 미치는 영향)

  • Shin, Dong-Hun;Kim, Hae-Ran;You, Young-Han
    • Journal of Wetlands Research
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    • v.14 no.1
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    • pp.1-9
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    • 2012
  • The effects of elevated $CO_2$ and temperature on the phenological and reproductive characteristics of Phytolocca insularis were examined in ambient condition (control) and green house situation (treatment), 700 ~ 800 ppm $CO_2$ and $2^{\circ}C$ elevated temperature, from March 2010 to April 2011. Phenological responses such as foliation, inflorescence formation, flowering, fruit appearance, fruit maturing of P. insularis grown in the treatment were 6~ 20 day faster than in the control. The percent of fruit set, number of fruit and seed per shoots, weight of fruit and seed per shoots of P. insularis were higher in control than in the treatment. The number of inflorescence per shoots showed no difference between in the control and in the treatment. These results demonstrated that the reproductive response of P. insularis might be negatively influenced by increased $CO_2$ concentration and elevated temperature.

Change in the Seed Characteristics and Germination Properties of Ulmus davidiana var. Japonica According to Seed Collection Time (채취 시기에 따른 느릅나무의 종자 형질 및 발아 특성 변화)

  • Tak, Woo Sik;Choi, Chung Ho;Kim, Tae Su
    • Journal of Korean Society of Forest Science
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    • v.95 no.3
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    • pp.316-322
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    • 2006
  • This study was conducted to examine the changes of seed characteristics and germination properties and to determine the most suitable collection time in Ulmus davidiana var. japonica. Seeds were collected six times with the interval of seven days after April 21, 2005 at Jeong-seon. There were differences in seed characteristics and germination properties among seed collection times. The sizes of seed and fruit increased with the collection time, but seed moisture contents decreased with the collection time after increasing until May 5. The 1,000 seeds weight decreased with the collection time after increasing until May 12. Collection time showed high positive correlations (r=0.790, p<0.01) with seed size and negative correlations (r=-0.919, p<0.01) with moisture contents. Percent germination (PG) presented the highest value in seeds collected at May 5, and decreased with the collection time. Mean germination time(MGT), germination speed(GS), and germination performance index(GPI) also showed the highest values in seeds collected at May 5. PG, MGT, and GS had correlations with other seed characteristics except the 1,000 seed weight. The relative growth rate of height and root collar diameter of seedlings presented the highest values in seedling germinated from seed collected at May 5, and seedlings germinated from seeds collected at May 5 and 12 showed the highest values in chlorophyll contents. Consequently it will be possible to product a number of seedlings when the seeds of U. davidiana var. japonica are collected just before drying morphologically.

Morphological Characteristics Based on Phenotypes of Flower and Fruit in a New Variety of Anthoxylum schinifolium (산초나무(Zanthoxylum schinifolium) 신품종의 꽃과 열매에 기반한 형태적 특성 분석)

  • An, Mi Yun;Song, Hyun Jin;Kim, Ki Yoon;Lee, Ro Young;Yang, Byeong Hoon;Han, Chi Beok
    • Korean Journal of Plant Resources
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    • v.35 no.4
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    • pp.464-470
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    • 2022
  • In this study, the morphological characteristics of flower and fruit in five varieties (Hancho 915ho, 930ho, 10ho, 1020ho, 1030ho) from Zanthoxylum schinifolium were investigated as new varieties with different fruiting seasons. Qualitative and quantitative characteristics were investigated, spectively. For quantitative characteristics can be measured numerically, such as size and number, the varieties were identified using ANOVA and Scheffe's multiple range test. In addition, the validity of whether the quantitative characteristics of five varieties can be explained by factor analysis was reviewed. Using UPGMA, the relationship between the five varieties was confirmed by dendrogram. As a result of ANOVA and Scheffe's multiple range test there was significant difference in inflorescence width and female flower length. It had a high contribution to distinguishing varieties, which was similar to the PCA results using factor analysis. As a result of dendrogram using cluster analysis, Hancho 915ho, 1020ho and 1030ho were similar, but there was a distinct difference in the characteristics of different flowering and fruiting periods, confirming that they were different varieties.

Monographic Study of the Endemic Plants in Korea V. Taxonomy and interspecific relationships of the genus Filipendula (한국 고유식물의 종속지 V. 터리폴속 식물의 분류와 종간유연관계)

  • 김기중
    • Journal of Plant Biology
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    • v.29 no.1
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    • pp.19-40
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    • 1986
  • A study on the species description, enumeration and interspecific relationships of genus Filipendula was undertaken for four Korean and two Japanese species. The use of scanning electron miroscope(SEM) has made possible a detailed study of foliar trichomes, pollen grains and fruit surface features. A new method for the preparation of SEM samples was developed in this study. Filipendula glaberrima, F. formosa, and F. korean were reliable endemic species. Among them, F. glaberrima shares many characteristics with F. formosa. F. gloaberrima can be divided into tow types based on leaf, seed, flower and pollen characters. Type I is characterized by ciliated fruits, many branched vascular bundles on the petal, dense trichomes on the leaf vein of abaxial surface, and many foveolate pollen surface. Type II possesses glabrous fruits, a few vascular bundles on the petal, rare trichomes on the leaf vein and a few foveolate pollen surface. The significant taxonomic characteristics in the classification of genus Filipendula are their lengths of fruit stalks, the densities of marginal trichomes of fruits, and textures of stipules.

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The Activation of PPAR-α and Wnt/β-catenin by Luffa cylindrica Supercritical Carbon Dioxide Extract

  • Kim, Bora
    • Natural Product Sciences
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    • v.25 no.4
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    • pp.341-347
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    • 2019
  • Luffa cylindrica (LC) is a very fast-growing climber and its fruit have been considered as agricultural wastes. We conducted to check the comparative qualities of ethanol solvent extraction (LCE) and supercritical carbon dioxide extraction (LCS) of L. cylindrica fruit and seed. LCS had higher antioxidant and polyphenol contents than LCE. LCS were significantly increased peroxisome proliferator-activated receptor (PPAR)-a and involucrin expression as epidermal differentiation marker in 3D skin equivalent model. LCS also showed antimicrobial activity against Staphylococcus aureus, a causative bacteria in atopic dermatitis. In addition, LCS inhibited the adipocyte differentiation of 3T3-L1 cells. When treated with the extract at a concentration of 100 ㎍/mL, the Wnt/β-catenin pathway reporter luciferase activity of HEK 293-TOP cells was increased approximately by 2-folds compared to that of the untreated control group. These results indicate that L. cylindrica supercritical carbon dioxide extract may serve as a cosmeceutical for improving skin barrier function and the treatment of obesity.

Development and industrial applications of versatile-usable genes of plant (식물 유용 유전자의 발굴 및 산업적 응용)

  • Oh, Boung-Jun
    • Proceedings of the Plant Resources Society of Korea Conference
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    • 2003.04a
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    • pp.40-60
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    • 2003
  • Fruit ripening represents a genetically synchronized system that involves developmental process unique to plant species, The phenomenon of ripening includes changes in color, texture, respiration rate, flavor, and aroma. Ripe fruits generally exhibit increased susceptibility to pathogen infection. However, fruits as a reproductive organ have their own protection mechanism against pathogens to maintain their integrity during seed maturation. In several nonclimacteric fruits, such as cherry, grape, and pepper, that do not have an ethylene burst during ripening, resistance against phytopathogens increases during ripening. Colletotrichum gloeosporioides is a causal agent of anthracnose disease in pepper plants (Capsicum annuum). We have established that C. gloeosporioides has susceptible and resistant interactions with pepper fruits during pre- and post-ripening stages, respectively. And we have interested in looking for a molecular mechanism that would explain the fungal resistance during ripening of nonclimacteric pepper fruit. In this presentation, a molecular characterization of the pepper esterase gene (PepEST) that is highly expressed in the resistant response will be demonstrated as an example of development and industrial applications of versatile-usable genes of plant.

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