• Journal of Embryo Transfer
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• v.28 no.3
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• pp.265-271
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• 2013

Cryopreservation and in vitro fertilization (IVF) protocols are important in genetic studies and applications to transgenic animals. Various studies about boar sperm cryopreservation have been studied for a long time. Those were about the use of extenders, the choice of sugars, the cooling and warming rates. The factors that influence the boar sperm are the dramatic changes in temperatures, osmotic and toxic stresses, and reactive oxygen species (ROS) generation. Among these factors, ROS generation is the main damage to DNA which is a principal genetic material and the most important for the practical applications. So we wondered whether ROS generation could be reduced. In previous study, monothioglycerol (MTG) was essential for the culture of embryo stem cells. Therefore we added MTG in the freezing extender based on lactose-egg yolk (LEY) with trehalose. For the assessment of the frozen-thawed sperm, we focused onmotility, membrane integrity and DNA damage. First, we used a computer-aided sperm analysis system for overall conditions of sperm such as motility and viability. Then we performed the sperm chromatin structure assay for DNA integrity and hypo-osmotic swelling test for membrane integrity. And our result showed the existence of MTG in the freezing extender caused less damage to DNA and higher motility in frozen-thawed boar sperm. Also we checked a relative antioxidant activity of MTG in modified Modena B extender. We concluded that this reagent can activate sperm mitochondria at MTG $0.2{\mu}M$, contribute to sperm motility and DNA integrity but there was no significant difference on membrane integrity. Also antioxidant activity of MTG in modified Modena B extender was proved.

• Animal Bioscience
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• v.34 no.6
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• pp.985-992
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• 2021

Objective: Artificial insemination plays an important role in genetic improvement in the goat farming system. The aim of this study was to investigate the effect of cervical application of hyaluronan (HA) on the fertility in goats after cervical artificial insemination using frozen-thawed (F-T) semen. Methods: After oestrous synchronisation with progesterone sponges and pregnant mare serum gonadotropin injection, both nulli- and multi-parous goats, were randomly allocated to 2 groups, and were inseminated with 0.25 mL of F-T semen (150×106 spermatozoa) twice at 52 h and 56 h after sponge removal. Prior to the insemination, goats in Group 1 only were given topical cervical HA application at 48 h after sponge removal. Site of insemination was recorded as os-cervix or intra-cervix or intra-uterus. Pregnancy was tested ultrasonographically 42 days after insemination. The data on pregnancy rates and percentage of animals according to the site of semen deposition were compared by Chi-square analysis. Results: The overall pregnancy rate was significantly (p<0.004) higher in goats with prior application to the cervix with HA (63.3%) than without (36.0%). Same pattern was observed in the pregnancy rates of nulli- and multi-parous goats in both the groups. Percentage of nulliparous goats according to the site of insemination in the HA group did not differ between first and the second insemination. However, in multiparous goats the percentage of animals inseminated intra-cervically was significantly increased (p≤0.05) between the first and the second inseminations. Conclusion: The results suggest that significantly higher fertility rate in the "HA goats" compared to the "without HA" group was because of deeper insemination facilitated by topical cervical application of HA. The deeper insemination into the cervical canal increase the rate of fertilisation when the cervical artificial insemination is performed.

• Proceedings of the Korean Society of Propulsion Engineers Conference
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• v.y2005m4
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• pp.413-422
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• 2005

In this paper, numerical results for 3-D supersonic turbine flow have been firstly compared with the experimental results to verify results computed by $Fine^{TM}/Turbo$. It was found that $Fine^{TM}/Turbo$ can accurately predict flow characteristics within supersonic turbine. Next, an grid system for 3D turbine flow was optimized selected through grid independency test. Finally the effect of axial gap between rotor and nozzle and chamfer angle of blade edge on the flow characteristics within 3-D supersonic turbine was analyzed with Frozen Rotor method.

• The Korean Journal of Pain
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• v.35 no.2
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• pp.152-159
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• 2022

Background: Subjects with frozen shoulder (FS) might not be comfortable with vigorous physical therapy. Clinical trials assessing the effect of graded motor imagery (GMI) in FS are lacking. The aim of this study was to determine the effect of GMI as an adjunct to conventional physiotherapy in individuals with painful FS. Methods: Twenty subjects aged 40-65 years having stage I and II of FS were randomly divided into two study groups. The conventional physiotherapy group (n = 10) received electrotherapy and exercises while the GMI group (n = 10) received GMI along with the conventional physiotherapy thrice a week for 3 weeks. Pre- (Session 1) and post- (Session 9) intervention analysis for flexion, abduction, and external rotation range of motion (ROM) using a universal goniometer, fear of movement using the fear avoidance belief questionnaire (FABQ), pain with the visual analogue scale, and functional disability using the shoulder pain and disability index (SPADI) was done by a blinded assessor. Results: Statistically significant difference was seen within both the groups for all the outcomes. In terms of increasing abduction ROM as well as reducing fear of movement, pain, and functional disability, the GMI group was significantly better than control group. However, both groups were equally effective for improving flexion and external rotation ROM. Conclusions: Addition of GMI to the conventional physiotherapy proved to be superior to conventional physiotherapy alone in terms of reducing pain, kinesiophobia, and improving shoulder function for stage I and II of FS.

• Journal of Advanced Marine Engineering and Technology
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• v.33 no.4
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• pp.443-450
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• 2009

This study has dealt with the ice making characteristics in a rectangular vessel cooled from below direction with experiment and numerical analysis. The experiment and numerical analysis were carried out under the following conditions which are the cooling wall temperatures of -5[$^{\circ}C$], -10[$^{\circ}C$], and -15[$^{\circ}C$]. The temperature profile of non-frozen layer was calculated by numerical analysis as the form of non-dimensional temperature. From this study, it is cleared that the existence of natural convection is clearly known. And also the non-dimensional freezing amount was derived from experimental result. This correlation equation will give a useful information to the designers of ice making system.

• Journal of Power System Engineering
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• v.11 no.3
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• pp.22-28
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• 2007

Liquefied gas vaporizer is a machine to vaporize liquefied gas such as liquid nitrogen($LN_{2}$), liquefied natural gas(LNG), liquid oxygen($LO_{2}$) etc. For the air type vaporizer, the frozen dew is created by temperature drop (below 273 K) on vaporizer surface. The layer of ice make a contractions on vaporizer. The structure analysis on the heat transfer was studied to see the effect of geometric parameters of the vaporizer, which are length 1000 mm of various type vaporizer. Structure analysis result such as temperature variation, thermal stress and thermal strain have high efficiency of heat emission as increase of thermal conductivity. As the result, Frist, With-fin model shows high temperature distribution better than without-fin on the temperature analysis. Second, Without-fin model shows double contractions better then with-fin model under the super low temperature load on the thermal strain analysis. Third, Vaporizer fin can be apply not only heat exchange but also a stiffener of structure. Finally, we confirm that All model vaporizer can be stand for sudden load change because of compressive yield stress shows within 280 MPa on thermal stress analysis.

• Journal of the Korean Society for Aeronautical & Space Sciences
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• v.36 no.3
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• pp.244-249
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• 2008

In this paper, the stable region for missile guidance loop employing an integrated proportional navigation guidance law is derived. The missile guidance loop is formulated as a closed-loop control system consisting of a linear time-invariant feed-forward block and a time-varying feedback gain. By applying the circle criterion to the system, a bound for the time of flight up to which stability can be assured is established as functions of flight time. Less conservative results, as compared to the result by Popov criterion, are obtained.

• Transactions of the Korean Society of Automotive Engineers
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• v.24 no.1
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• pp.10-15
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• 2016

This research was conducted structural analysis in order to analyze the impact of the gear in Urea pump when the water is frozen. Subject of study, gear was designed nine models, this gear is a spur gear, located in pump. Contact conditions and rotation conditions were set the gear's condition of restriction. Given 136 MPa pressure to external gear by water was set to the applied stress. The performing result of structural analysis, maximum stress and strain are appeared between two gears. At the same diameter, strain and stress are decreased gradually thicker. Because of the little part in crevice between gears, this parts of gears could be obtained conclusion to be generated maximum stress and strain.

• Journal of Biosystems Engineering
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• v.39 no.4
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• pp.330-335
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• 2014

Purpose: This study was conducted for enhancing the performance of a conventional quick freezer by introducing the supercooling state, using a low-temperature coolant. Methods: In the present investigation, the supercooling process was executed prior to quick freezing for reducing the time by which the temperature passes the zone of maximum ice crystal formation. Every food has different nucleation points and hence, we used silicone oil as the coolant for supercooling for easy modification of temperature. Additionally, for quick freezing, we used liquid nitrogen spray. Results: Using the heat exchanger-type precooler with silicone oil, the temperature of the chamber was easily changed for enabling supercooling. Particularly, the results of the freezing test with garlic indicated that this system improved the hardness of garlic after it was thawed, compared to the conventional freezing method. Conclusions: Before quick freezing, if the food item is subjected to the supercooling state, the time from nucleation to the temperature reaching the frozen state ($-5^{\circ}C$, which is the maximum ice crystal formation zone) will be shorter than that incurred using quick freezing alone. The combination of the heat exchanger-type supercooler and liquid nitrogen sprayer is expected to serve as a promising technology for improving the physicochemical qualities of frozen foods.

• The Journal of Fisheries Business Administration
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• v.38 no.1 s.73
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• pp.115-132
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• 2007

Implementing the automatic identification in supply chain management is essential for effective and efficient process control. Though the GTIN based bar code system is generally used as an automatic identification method in most industries, it can not identify individual item, and is not appropriated for products' reliability and safety management. So the RFID system with EPC is considered as a better solution for resolving those problems. This study reviewed automatic identification code systems and the attributes and characteristics of RFID Gen 2 which became a global standard recently for supply chain management. Particularly, this study analyzed RFID Gen 2 systems' read rates on various conditions including distances between tags and readers and between antennas, condensation, and several packing materials in practical supply chain environment. The results of this study showed that the RFID Gen 2 had high read ratio in practical application and would be adopted as a new automatic identification means for traceability systems.