• The Korean Journal of Mycology
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• v.46 no.1
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• pp.1-8
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• 2018

Three fungal isolates, NNIBRFG108, 1139, and 1480, were isolated from freshwater environments; NNIBRFG108 from plant litter in Samcheok, Gangwon and NNIBRFG1139 and 1480 from the soil in Jeju & Gimcheon, Gyeongbuk, Korea. Based on the morphological characteristics and phylogenetic analysis of internal tanscribed spacer (ITS), 28S rDNA region, and ${\beta}$-tubulin gene, NNIBRFG108, NNIBRFG1139, and NNIBRFG1480 isolates were confirmed as Didymella segeticola, D. ellipsoidea, and D. aeria, respectively. Neither species has previously been described in Korea.

• Mycobiology
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• v.28 no.4
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• pp.197-201
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• 2000

The distribution and occurrence of aquatic zoosporic and terrestrial fungi were investigated in 21 well waters in Assiut governorate, Egypt. Using a zoospore capture technique, 923 colonies of aquatic freshwater fungi were recovered from well waters, out of which 811 colonies reached sexual maturity. These colonies were assigned to 23 species which belong to 11 genera. The most common genera were Achlya, Saprolegnia and Dictyuchus. Using two types of media, 35 species in addition to 2 varieties of terrestrial fungi which belong to 18 genera were also recovered. The most frequent glucophilic genera (recovered on glucose-Czapek's agar at $28^{\circ}C$) were Aspergillus, Penicillium and Fusarium. The results obtained on cellulose-Czapek's agar at $2^{\circ}C$ were basically similar to those on glucose agar and the most frequent genera were Aspergillus, Penicillium and Fusarium followed by Chaetomium and Cephalosporium.

• Mycobiology
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• v.46 no.3
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• pp.205-214
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• 2018

The order Mucorales, the largest in number of species within the Mucoromycotina, comprises typically fast-growing saprotrophic fungi. During a study of the fungal diversity of undiscovered taxa in Korea, two novel mucoralean strains, CNUFC-GWD3-9 and CNUFC-EGF1-4, were isolated from specific habitats including freshwater and fecal samples, respectively. On the basis of their morphological characteristics and sequence analyses of internal transcribed spacer and large subunit ribosomal DNA, the CNUFC-GWD3-9 and CNUFC-EGF1-4 isolates were confirmed to be Gilbertella persicaria and Pilobolus crystallinus, respectively. It is ecologically, pathologically, and mycologically significant to find such rare zygomycetous fungi in such specific habitats.

• The Korean Journal of Mycology
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• v.50 no.3
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• pp.217-224
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• 2022

This study aimed to isolated wild yeasts from freshwater in Korea. The strains were identified by using the D1/D2 domains of the 26S rDNA regions. Consequently, six strains were named as Apiotrichum chiropterorum (NNIBRFG36995), A. domesticum (NNIBRFG32938), A. dulcitum (NNIBRFG33144), A. laibachii (NNIBRFG36991), Saprochaete quercus (NNIBRFG33183) and Tausonia pullulans (NNIBRFG33181). These yeasts have not previously been recorded in Korea, this paper is the first report. We were investigated to the morphological and cultural characteristics of these yeasts. All the strains grew well on Yeast extract peptone dextrose (YPD), Potato dextrose (PD), and Yeast mold (YM) media and in temperature range of 15-30℃. A. domesticum (NNIBRFG32938), A. laibachii (NNIBRFG36991) and T. pullulans (NNIBRFG33181) grew even in 20% glucose containing YPD medium, so they had glucose tolerance. A. domesticum (NNIBRFG32938) and A. laibachii (NNIBRFG36991) had salt resistance as growing even in 5% NaCl containing YPD medium.

• The Korean Journal of Mycology
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• v.43 no.2
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• pp.92-98
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• 2015

Hydrocharis dubia Backer and Salvinia natans All. were sampled from the Junam and Dongpan reservoirs, representative freshwater wetlands of Korea. A total of 19 endophytic fungal strains were isolated from hydrophytes native to the Junam wetlands and 5 strains were isolated from the Dongpan wetlands. Depending on phylogenetic analysis based on internal transcribed spacer (ITS) region, strains from Junam belonged to 11 genera and from Dongpan belonged to 5 genera. Fusarium, Phoma and Talaromyces were commonly distributed genera from two wetlands. The fungal diversity index showed clear differences between each wetlands or each host hydrophyte. Above all, the highest diversity value was observed from Salvinia natans All., which have been reported as promising biological resources as eutrophication controller in environmental ecology.

• Mycobiology
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• v.45 no.4
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• pp.318-326
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• 2017

In a survey of undiscovered taxa in Korea, three zygomycete fungal strains-EML-W31, EML-HGD1-1, and EML-RUS1-1-were isolated from freshwater, grasshopper fecal, and soil samples in Korea. On the basis of the morphological characteristics and phylogenetic analysis of internal transcribed spacer and 28S rDNA, the isolates of EML-W31, EML-HGD1-1, and EML-RUS1-1 were confirmed to be Cunninghamella bertholletiae, Cunninghamella echinulata, and Cunninghamella elegans, respectively. These species have not been previously described in Korea.

• Mycobiology
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• v.46 no.4
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• pp.317-327
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• 2018

While surveying undiscovered fungal taxa in Korea, three rare zygomycetous fungal strains, CNUFC-PTF2-1, CNUFC-TF3-1, and CNUFC-ESAF3-1, were isolated from soil, leaf, and freshwater samples, respectively. The strains were analyzed morphologically as well as phylogenetically based on the internal transcribed spacer region and 28S rDNA sequences. Sequence analysis of the two loci revealed that the isolates, CNUFC-PTF2-1, CNUFC-TF3-1, and CNUFC-ESAF3-1, were identified as Backusella circina, Circinella muscae, and Mucor ramosissimus, respectively. These species have not yet been previously described in Korea.

• Mycobiology
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• v.48 no.5
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• pp.410-417
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• 2020

Fungal ecological interactions play a key role in structuring community assemblages. These associations may involve both antagonistic and synergistic relationships, which are commonly influenced by abiotic factors such as nutrient conditions. However, information for extreme, oligotrophic systems remain poor. Herein, interactions among key members of the aquatic transient fungal community (Aspergillus niger, Cladosporium sp., and Coprinellus micaceus) of a low-nutrient freshwater system in the Cuatro Ci enegas Basin, Mexico were studied. Pairwise interaction bioassays were explored in vitro under different nutrient conditions, including carbohydrates-rich, carbohydrates and amino peptides-rich, and low nutrients. Our results indicated that antagonistic patterns prevail among the studied taxa. However, nutrient-dependent changes were observed in Cladosporium sp. shifting to synergy under carbohydrates-rich conditions, suggesting changes in the fungal community composition as a result of nutrient enrichment. Remarkably, our findings contrast with previous work demonstrating mainly synergistic interactions between our tested fungal isolates and co-occurring autochthonous bacteria (Aeromonas spp. and Vibrio sp.) under low-nutrient conditions. This observation may indicate that bacteria and fungi exhibit distinct community-level responses, driven by nutrient conditions. This contributes to the knowledge of fungal community dynamics and interspecific interactions in an oligotrophic ecosystem, highlighting the relevance of nutrient-based shifts and antagonistic interactions in ecosystem dynamics.

• Mycobiology
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• v.48 no.1
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• pp.29-36
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• 2020

During an investigation of fungi of the order Mucorales from freshwater and sediment samples in Korea, we isolated six strains, NNIBRFG6649, NNIBRFG6255, NNIBRFG1498, CNUFC-YJ13, CNUFC-YR7, and NNIBRFG2739. The morphology and phylogeny of these strains were analyzed. Based on the morphological characteristics and molecular data from internal transcribed spacer (ITS) region, the isolates NNIBRFG6649 and NNIBRFG6255 were identified as Mucor abundans, and M. aligarensis, respectively. The isolates NNIBRFG1498 and CNUFC-YJ13 were identified as M. moelleri, whereas the isolates CNUFC-YR7 and NNIBRFG2739 were identified as M. heterogamus. To the best of our knowledge, M. abundans, M. aligarensis, M. moelleri, and M. heterogamus have not yet been reported in Korea.

• Mycobiology
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• v.45 no.4
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• pp.344-352
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• 2017

During a survey of fungal diversity of the order Mucorales, three zygomycete isolates, CNUFC-YR113-1, CNUFC-KNU16-7, and CNUFC-BS1-1 were isolated from freshwater and soil samples in Korea. The strains were analyzed both morphologically and phylogenetically based on internal transcribed spacer and 28S rDNA gene sequences. Based on their morphology and phylogeny, the CNUFC-YR113-1 and CNUFC-KNU16-7 isolates were identified as Actinomucor elegans, and CNUFC-BS1-1 was identified as Mucor minutus. To the best of our knowledge, the species A. elegans and M. minutus, belonging to an undiscovered taxon, have not been previously described in Korea.