• Journal of Mushroom
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• v.20 no.3
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• pp.147-152
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• 2022

To develop mushroom varieties for cultivating at low temperature on oak logs, a strain with a low fruiting body generation temperature was crossed with Di-mon to select for a line with excellent properties. Selection was followed by cultivation testing. From these studies, Heunghwa 1ho was identified. The optimum temperature for cultivating Heunghwa 1ho, was 13.3℃. The fruiting temperature range was 6.4~20.2℃, identical to that of the parent strain. Growth at 25℃ for 7 days achieved optimal mycelial growth of 61.9±2.10 mm, superior to growth of the parent strain at this temperature. The cap shape of Heunghwa 1ho was convex, cap diameter was 57.8±8.31 mm, and cap color was brown. Heunghwa 1ho showed similar genetic traits to those of the parental strain. However, dry weight (20.1 kg/m³) and cap diameter and color are superior to those of the parent strain. The 3 year fresh oak mushroom yield was 113.8 kg/m³, superior to the respective yields of the parent strains JMI 10047 and JMI 90021 (92.5 kg/m³ and 66.4 kg/m³).

• Journal of Mushroom
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• v.16 no.4
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• pp.338-341
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• 2018

This study investigated the possibility of using pine tree sawdust in thinning-out tree as a substitute for poplar and douglas-fir tree sawdust in oyster mushroom cultivation. Mycelial growth was 10.8 cm in medium supplemented with 70% pine sawdust after 18 days of culture and 10.2 cm in control medium. Mycelial density showed high density with no significant difference between treatments. Fresh weight and diameter of fruiting bodies were slightly lower in medium supplemented with pine tree sawdust. The hardness of pine tree sawdust was slightly higher in the medium supplemented with 20% and 30% of pine tree sawdust. The L value showed a tendency to be higher in the pine added medium, but the a and b values d id not show any significant difference between the treatments. The yields of fruiting body were 157 g / 850 ml for the control and 170 g / 850 ml for the 40% added pine tree sawdust.

• Journal of Mushroom
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• v.20 no.4
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• pp.258-262
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• 2022

Pleurotus species are the most consumed and cultivated mushrooms in Korea. Although oyster mushrooms (P. ostreatus) can be cultivated automatically, their storability is slightly lower than that of king oyster mushrooms (P. eryngii) and winter mushrooms (Flammulina velutipes); therefore, the export proportion of oyster mushrooms is very low. Since Korean mushrooms are highly preferred across Southeast Asian, the export of oyster mushrooms in the form of complete substrates is expected to be more promising than that of fresh mushroom. Here, 1 and 2.5 kg complete substrates of P. ostreatus 'Soltari' and P. sajor-caju 'Sambok' were prepared and stored at different temperature from 0 to 15℃ for 10 days. Thereafter, the formation of fruiting bodies was induced. Since the 2.5 kg complete substrates required 70 days of incubation, their mycelia were at an advanced age and their fruiting bodies did not grown normally. When 70%-incubated complete substrates were stored at 5-10℃, the growth was faster and more uniform and stable fruiting bodies were formed. Export test of complete substrates to Vietnam using distribution containers set at 0℃ and 15℃ revealed that the growth period was shortened by 1-2 days when the distribution containers were set at 15℃ and the yield of 'Soltari' increased by approximately 10%. In addition, even though the yield of 'Sambok' was similar between treatments at 0℃ and 15℃, the quality of fruiting bodies from 15℃-distributed complete substrates was much better than that of those from 0℃-distributed substrates.

• The Korean Journal of Mycology
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• v.3 no.1
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• pp.1-19
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• 1975

Since the importance of casing in fruit body formation of Agaricus bisporus has been emphasized, physico-chemical characteristics of casing materials were discussed by many workers and a mixture of peat and mineral soil as proper casing material has been adopted in many of mushroom growing countries. Because of limited resources of peat in Korea, it is necessary to find practical performance and substitutional materials for casing. The effect of casing on mycelial growth and mushroom yield of A. bisporus varied with materials, its combination and practices etc. The experiments to be discussed in this paper are concerned with pH and Ca of casing material which influence A bisporus, and changes of physico-chemical characteristics with mixing ratio of casing materials and its effect on A. bisporus. The optimum range of moisture content of each material, management of watering and application of physico-chemical characteristics casing materials was also investigated and re-use of weathered spent compost for casing material was described. 1. The effect of calcium on mycelial growth of A. bisporus at various pH in Halbschalentest showed different results with calcium sources. Best results were obtained around neutrality and fresh weight of fruit bodies grown in the range of pH 7 to 8 was highest among the tested levels. 2. Available moisture, pore space, organic matter, cation exchangeable capacity and exchangeable cation was increased by an increase of mixing ratio of peat in casing materials, while an adverse effect was obtained by addition of sand. 3. Mycelial growth on clay loam was more rapid at a lower bulk density of 0.75g/cc and at 20% moisture content on a dry weight basis at the same bulk density. 4. Mixing ratio of casing materials, 60 to 80 per cent by volume of peat mixed with 20 to 40 per cent of clay loam produced the highest yield of fresh fruit bodies and sand the lowest. However, per cent of open cap was highest in peat and lowest in sand. 5. Days required for fruit body initiation was shortened in mixtures of peat and clay loam by one to three days compared with other materials and the formation of flushes was clear. 6. The effect of some physico-chemical characteristics of casing materials on the fresh weight of fruit bodies were estimated by a multiple regression equation; Y=-923.86+$8.18X_1+8.04X_2+7.90X_3+0.12X_4+2.03X_5-0.82X_6-0.54X_7$ where $X_1,X_2,X_3,X_4,X_5,X_6,X_7$ are sand, silt, clay, available moistuer, porosity, organic matter and exchangeable cation respectively. The productivity of certain casing material could be predicted from this equation. 7. Fresh weight of fruit bodies was positively correlated with porosity exchangeable cation, organic matter, available moisture, silt and clay of materials; while sand was negatively correlated. On the contrary, sand was the unique factor reducing per cent of open cap. 8. Distribution of three phases of high productive casing material was concentrated in the range of 10 to 30 per cent solids, 15 to 30 per cent liquids, and 50 to 60 per cent in air volume. 9. Fresh weight of fruit bodies from peat was not affected with heavy watering but in clay loam and sandy loam severe crop losses occurred. Fresh weight of individual fruit was increased and open caps were decreased with heavy watering but light watering resulted in adverse effects: its effect was especially great in peat. 10. Optimum range of moisture content by weight on a dry basis was different with each casing material. To maintain optimum moisture content concerned with yield of fruit bodies and open cap, sandy loam and peat mixtures required daily watering of 0.6, 0.6 to 1. 2 and 1.2 to 2.4 liters per $3.3m^2$ of bed area, respectively. 11. Maximum yield of fruit body was recorded in the range of pF 2. 0 to 2. 5 of casing materials if organic matter content was below 4.2 per cent and in pF 1. 3 to 1.8 if above 7.1%. 12. pF curve of a certain casing material could be draws from moisture content at various pF values by multiple regression equations provided texture, organic matter and calcium of the casing material are given. Optimum moisture range of the casing materials also could be estimated by the equation. 13. It was possible to improve the phyico-chemical characteristics of clay loam and sandy loam by addition of weathered spent compost although the effect was less than in the case of peat. Fresh weight of fruit bodies wsa increased by addition of weathered spent compost but its effect was not as remarkable as peat. Accordingly, further studies will be required.

• The Korean Journal of Mycology
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• v.20 no.3
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• pp.273-276
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• 1992

Armillaria mellea mushrooms were cultivated on the sawdust media, Quercus sawdust; rice bran=80:20 in polypropylene bags. The isolate of Armillaria mellea used was ARM69002F collected from a Korean pine plantation in Hongcheon district. The length of time between spawning and fruiting was required for 90 to 100 days. The number of fruiting bodies produced in a bags with a kg substrate were approxinately 31 (range of 18 to 62), and the total fresh weight 158g (61 to 207g), converted to 13 to 15% of fresh weight. The pilei of fruiting bodies were average 4.0 cm (2.5 to 7.6 cm)wide, and their stipes 8.2 cm long and 0.6 cm-thick at the upper part and 1.2 cm at the base.

• The Korean Journal of Mycology
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• v.3 no.2
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• pp.19-23
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• 1975

A study on the variation of the air temperature in the pine mushroom box containing dry ice for the preservation of the freshness of the pine mushroom, Tricholoma matsutake (Ito et Imai) Sing. produced in Korea was made in Seoul, Korea during the period of August 1 to 30, 1975. A safe shipment of 5 kg (net weight) of fresh mushrooms with freshness preserved for 24 hours in August required the placement of 4.5 kg of dry ice-a half in the upper portion and the other half in the middle portion-in an airtight container (mushroom box). The container used for the shipment test, made of 3mm-thick double-layer cardboard, was of the dimensions : $0.33{\times}0.4{\times}0.5=0.066(m^3)$. As the temperature fell, decreased amounts of dry ice were needed : 3 to 2 kg in September and 1.5 to 0.3 kg in October. With this preservation method, large quantities of fresh mushrooms were exported to Tokyo ana Osaka, Japan without damage in the process of transportation.

• The Korean Journal of Mycology
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• v.16 no.4
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• pp.204-206
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• 1988

Chemical analysis of both sclerotia and the fruits of Pleurotus tuber-regium showed higher values for such elements as calcium(Ca), iron(Fe), zine(Zn) in the fruits than in the sclerotia. On the contrary magnessium(Mg) was found to be higher in sclerotia than in the fruits. protein and carbohydrate were also found to be more in the fruits. There was no significant difference between the chemical values of old(1 year) and fresh sclerotia. Oil palm fruit fibre substrate produced sporophores with higher values for the minerals, protein and carbohydrate than those on riversand substrate.

• Journal of the Korean Society of Food Science and Nutrition
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• v.41 no.6
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• pp.829-833
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• 2012

To investigate the effects of oyster mushrooms (Pleurotus ostreatus) on beef and pork tenderization, freezedried mushroom (whole, cap, and stem) powder and mushroom extracts were prepared. Both fresh and boiled beef and pork had mushroom power and extract powders spread on them, and let sit for 30 minutes at room temperature. After that, the hardness and amino nitrogen content of the meats were measured. The hardness of the meat decreased by about 20%~35% after spreading. Also, the amino nitrogen contents increased due to the effect of protease in the mushrooms. The effects were greater in raw beef. The mushrooms showed almost the same effects regardless of which part of the mushrooms were used (whole, cap, and stem).

• Mycobiology
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• v.36 no.3
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• pp.157-160
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• 2008

This study was carried out to determine the possibility of bottle cultivation utilizing recycled oyster mushroom culture waste as a cultivating substrate for P. ostreatus. Total nitrogen percentage was 0.76%, 1.13%, 1.16%, 1.36%, and 1.38% in the 1-, 2-, 3-, 4-, and 5-time mixed substrate, respectively; 0.95%, 1.04%, 1.34%, 1.36%, and 1.25% in the 1-, 2-, 3-, 4-, and 5-time postharvest substrate, respectively; and 0.72% and 0.68% in the 2- and 3-time nonadditive substrate, respectively. Weight of the fresh fruiting body harvest was 115 g, 120 g, 117 g, 118 g, and 114 g on 1-, 2-, 3-, 4-, and 5-time mixed substrate, respectively; and 105 g and 45 g on 2- and 3-time nonadditive substrate, respectively. The first mixed substrate (fresh) and recycled substrates generated no significant difference in the weight of fresh fruiting bodies harvested.

• Journal of Mushroom
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• v.12 no.2
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• pp.138-143
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• 2014

This study was conducted to know the optimal supplementary level of spent mushroom substrates (Flammulina velutipes) as an energy source and fermentation period in manufacturing of rye silage. Whole crop rye was harvested at full bloom stage and ensiled with spent mushroom substrates of 20%(S-20), 40%(S-40) and 60%(S-60) as fresh matter basis. Each silage was prepared in plastic buckets included with vinyl bag by three replications and fermented for 3, 6 and 9 weeks, respectively. Moisture contents of whole crop rye at full bloom stage and spent mushroom substrates were 62.3% and 54.3%, respectively, and those of silages was ranged from 58% to 64%. Ether extracts content of silages was significantly (P<0.05) increased in 6 weeks of fermentation period. The pH of silages ranged from 4.46 to 5.05, and fluctuated in the changes by fermentation period beside of decreased with elapsing the period in the S-60. The organic acid content of silages was higher in the order of lactic acid, butyric acid, acetic acid and propionic acid. Lactic acid content was higher when fermented for 6 weeks compared to the other fermentation period. Flieg's score for estimation of silage quality ranged from 63 to 80, and was relatively high level in the S-20 and the S-40 fermented for 6 weeks, and was high level in S-20, and was relatively low level in the silages fermented for 3 week. Hence, we suggest that supplementation of 20% spent mushroom substrates in fresh matter basis and fermentation for 6 weeks are resonable for a good quality of rye silage.