• Magazine of the Korean Society of Agricultural Engineers
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• v.31 no.2
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• pp.43-53
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• 1989

In this research programs, a series test was conducted to show the effects of freeze/thaw process on the various soil properties. The tests were carried out taken from the west sea shore of Korean peninsular and the west sea shore of Scotland, and their results are as follows; 1. There was a positive total heave in a freezing run, although water may he expelled for the sample initially. The water flow must he reverse' from expulsion to intake. 2. The confining pressure had an overriding influence on the heave and frost penetration, a sudden change of the axial strain at failure with strain rate was observed occuring at a strain rate between 10-5 and 10-6, and the initial friction angle of frozen clay was appeared zero. 3. There was shown a significant decrease in liquid limit of soil which was subjected to freeze/thaw process for the initial value of about 20% because of soil particles aggregation. 4. The cyclic freeze/thaw caused a sinificant reduction in shear strength and its thixotropic regain. The frozen/thawed soil exibited negative strength regain, particularly at high freeze/thaw cycles. 5. The freezing temperature greatly influenced on the failure strength of soils and this. Trend was more pronounced the lower the freezing temperature and shown the ductile failure with indistinct peaks.

• Korean Journal of Veterinary Research
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• v.34 no.4
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• pp.851-855
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• 1994

The semen from four male dogs which had been proven to be fertile in the past were frozen in a deep freezer at $-60^{\circ}C$ by simple freezing method using methanol and preserved at the same teperature for from 7 to 10 days. The semen were inseminated to 7 female dogs in estrus to find out the usability of this freezing method in artificial insemination for dogs. In addition, post-thaw motility and viability of sperm from two male dogs which had been fertile were also evaluated to investigate individual difference. Successful pregnancy was obtained by artificial insemination with canine semen frozen at $-60^{\circ}C$ by simple freezing method using methanol, namely, 3 bitches among 7 bitches which had been inseminated delivered puppies(42.8%). The average litter size of the whelping dogs were 4.3 puppies. The average post-thaw motility of canine sperm in the cases of conception was showen higher than those of non-conception(65.0% vs. 42.5%), along with the, same result in the average post-thaw viability between the two groups(53.3% vs, 27.5%). Individual difference of post-thaw motility and viability was obtained between two fertile dogs(p<0.05).

• Journal of Veterinary Clinics
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• v.21 no.4
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• pp.363-368
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• 2004

To investigate the effects of seeding during freezing procedure on post-thaw viability, motility, and acrosome integrity of boar spermatozoa, semen from 5 Yorkshire boars were collected for this experiment. Raw semen were diluted with Merck I, subsequently added with cooling diluent containing lactose and egg yolk and with freezing diluent containing glycerol. The diluted semen were frozen on the rack in the styrofoam box filled with liquid nitrogen at the distance of 5 cm or I cm above LN2 level. Seeding was performed to only a group of straws frozen at 5 cm away on the surface of LN2. The frozen semen were thawed in $50^{\circ}C$C water and the viability and local motility were analyzed by sperm analysis imaging system. A part of thawed semen was taken for the examination of morphology of apical ridge of the acrosome to compare with the effect of seeding between the seeding-treated and non treated groups. I. Post-thaw viability was considerably higher in seeding-treated sperm than non-seeding group (p<0.01), however, no difference of local motility was obtained among the groups. 2. At three hours after thawing, viability was also higher in seeding-treated group than non-treated group (p<0.05), along with no difference of motility among the groups. 3. Higher normal acrosome integrity was obtained in the seeding-treated sperm than non-treated groups (p<0.01). 4. Between non-seeded groups, higher normal acrosome integrity was obtained in the sperm group frozen at 5cm upper on the surface of LN2 than that frozen at 1cm away (p<0.01). These results indicated that seeding treatment during freezing boar spermatozoa was beneficial to post-thaw viability and normal acrosome integrity.

• Journal of The Korean Society of Agricultural Engineers
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• v.52 no.3
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• pp.89-95
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• 2010

There is the freezing method as one of the ground improvement methods for excavating an underground tunnel, and due to its improved reliability, recently construction cases of applying this method into sandy soil grounds as well as cohesive soil grounds of cities have been reported. But, applying the freezing method into cohesive soil grounds could bring concerns of the expansion of the whole ground and the settlements from thawing of ground. In this study, the deformation strength characteristics of cohesive soil which received freezing and thawing hysteresis were examined using the sample collected from the site of cohesive soil ground applied with the freezing method and its structural characteristics were analyzed using an electronic microscope. And, the test with cohesive soil reconstituted from cohesive soil which received freezing and thawing hysteresis was carried out and its result was analyzed comparatively. The result of this test showed that the structure of natural clay was significantly changed due to freezing and thawing hysteresis.

• Journal of the Korean Ceramic Society
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• v.27 no.3
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• pp.437-443
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• 1990

The resistivity of cement paste and mortar for freezing and thawing was investigated for densifying the structure of cement paste and mortor, slag, diatomaceous earth and fly ash as blending materials and superplasticizer were used, and air entraining agent was added to absorb the volume expansion by freezing and thawing reaction. And then the specimens were subjected to freeze-thaw in water. When both of air entraining agent and superplasticizer as additives were mixed to specimens, their freeze-thaw resistance was enhanced by the air entraining effect and the water reduction effect. When 4% of slag were added to cement, freeze-thaw resistance was especially excellent. In addition, it was found that the specimens with blending materials were more influenced by curing periods than those without admixture. It is assumed that the curing periods contribute to exibit the potential hydraulicity and pozzolanic reactivity of blending materials and to densify their texture.

• Geomechanics and Engineering
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• v.29 no.3
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• pp.349-357
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• 2022

Abnormal climate events are occurring frequently around the world. In particular, cold waves and heavy snow lead to damage and deterioration of facilities, which can cause loss of life or property damage, such as shortening the lifespan of facilities. Therefore, it is very important to prepare an appropriate maintenance system and to establish a strategy to cope with abnormal weather conditions. In this study, laboratory freezing experiments were performed to analyze the freeze-thaw characteristics affecting the tunnel concrete lining, and heat flow analysis was carried out based on the test results. Based on these experimental and theoretical analysis results, quantitative freeze-thaw evaluation criteria for tunnel concrete linings were proposed.

• Journal of Embryo Transfer
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• v.12 no.2
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• pp.141-149
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• 1997

This study was carried out to investigate the effect of vitrification and slow freezing methods on the post-thaw developmental rate of rabbit zygotes. After exposing rabbit zygotes in EFS solution for 0.5, 1, 2, 3 and S min at room temperature, they were washed with 0.5 M sucrose solution, D-PBS and TCM-199 and then cultured in TCM-199 plus 10% FBS with bovine oviduct epithelial cells(BOEC) to examine whether the cryoprotectant induced injury during the various exposure periods. The embryo development rates to hatched blastocyst after exposing in EFS solution for 3 and 5 min(40.0 and 16.7%) were significantly lower than in 0.5, 1 and 2 min(63.0, 72.0 and 54.5%), respectively. The post-thaw development rates to hatched blastocyst were significantly(P<0.05) higher in in vivo morula with intact mucin coat(85.2%) and mucin seperated morula(77.8%) than those of in vitro morula(58.5%) and zygote(5.9%), hut no difference was shown between in vitro morulae and mucin separated morula. The cryoprotectant dilution procedures showed no effects on the post-thaw development rates to hatched blastocyst under the present culture conditions. The post-thaw development to hatched blastocyst in the rabbit zygotes was not significantly different between the slow freezing(12.8%) and vitrification(5.9%). These results indicated that the rabbit frozen zygotes could he successfully developed in vitro to hatched blastocysts, though their developmental rate was very low, compared with morula stage embryos, in either vitrification or slow freezing procedure under the present conditions.

• Clinical and Experimental Reproductive Medicine
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• v.17 no.1
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• pp.81-85
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• 1990

We cryopreserved mouse morulae by a simple ultra-rapid method of freezing embryos directly in $LN_2$ after holding 2min in a $LN_2$ vapor, and thawed them in $37^{\circ}C$ water bath. The time requirements for permeation and dehydration by 2.0 M glycerol and 0.2 M sucrose before freezing were studied. When the embryos were equilibrated for 10 min, the optimun post-thaw survival was obtained. Embryos those developed normally to blastocyst after in vitro culture for over 24hrs were regarded as survival ones. Two experiments to assess post-thaw survival following predehydration in various mixtures of glycerol and sucrose were also accomplished. When sucrose was held constant (0.2 M) and glycerol concentration varied (1.5-3.5 M), post-thaw survival was best (78.0%) in 3.0 M glycerol. When glycerol was held constant (3.0M) and sucrose concentration varied (0.0-1.0M), optimun post-thaw survival (78.0%) was found in 0.2 M sucrose.

• Journal of the Korean Ceramic Society
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• v.28 no.7
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• pp.509-516
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• 1991

The effect of various polymers on the freeze-thaw resistance of hardened cement mortar was investigated. For this study, styrene butadiene rubber (SBR), ethylene vinyl acetate (EVA), polyvinyl alcohol (PVA) were used to prepare cement mortar specimen, and then freeze-thaw experiment was carried out. By adding SBR adn EVA to the specimen, the freeze-thaw resistance of specimens was improved, but when PVA was added to the specimen, its freeze-thaw resistance was lowered. Particularly, the specimens which were added 5, 10% of SBR and 5% of EVA showed excellent freeze-thaw resistance in the salt environment.

• Proceedings of the Korea Concrete Institute Conference
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• 2002.05a
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• pp.643-648
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• 2002

A research for recycling the demolished-concrete as concrete aggregate has been concerned in all over the world. There, however, are some problems that qualities of recycled aggregates are not only largely different, but also mechanical properties of recycled aggregate concrete decrease a little in comparison with that of natural aggregate concrete. In this study, the resistance of freezing and thawing of concrete using source-concrete recycled aggregate(SRN) and demolished-concrete recycled aggregate(DRA) was investigated. Futhermore a research for improvement of freeze and thaw durability of recycled aggregate concrete was performed. Relative dynamic modulus of elasticity of SRN and DRA recycled aggregate concrete was dropped 60% before 150 of freezing and thawing cycle, and was much lower than that of control concrete. Relative dynamic modulus of elasticity of recycled aggregate concrete was increased to decrease water-cement ratio, but the freeze and thaw durability of recycled aggregate concrete was not enough improved. Futhermore, when metakaolin and silica fume were repalced, the freeze and thaw durability of recycled aggregate concrete containg metakaolin was more improved than that of silica fume.