• Journal of Microbiology
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• v.41 no.3
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• pp.262-265
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• 2003

Although enumerating bacterial cells is a fundamental step in understanding microbial ecosystems in marine environments, substantial decrease in bacterial counts with increasing sample storage time hampers the accurate estimation of bacterial biomass. We compared the variations in bacterial cell numbers caused by freezing and thawing of sample bottles or slides. Bacterial counts of seawater samples frozen only once in a sampling bottle yielded approximately 95％ of the original numbers after 90 days, whereas 80％ of the original count was obtained for samples prepared on slides. Only 67％ and 58％ of the original counts were recovered in samples repeatedly frozen and thawed in bottles or on slides, respectively. The results indicated that freezing a seawater sample in a bottle increased the consistency of the epifluorescence microscopic enumeration of bacterial cells.

• Corrosion Science and Technology
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• v.6 no.4
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• pp.177-185
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• 2007

The purpose of this study is to evaluate freezing-thawing and surface scaling resistance in order to examine the frost durability of concrete in a chloride-inherent environment. The mixing design for this study is as follows: 3 water binder ratios of 0.37, 0.42, and 0.47; 2-ingredient type concrete (50% OPC concrete and 50% ground granulated blast-furnace slag), and 3-ingredient type concrete (50% OPC concrete, 15% fly ash, and 35% ground granulated blast-furnace slag). As found in this study, the decrease of durability was much more noticeable in combined deterioration through both salt damage and frost damage than in a single deterioration through either ofthese; when using blast-furnace slag in freezing-thawing seawater, the frost durability and surface deterioration resistance was evaluated as higher than when using OPC concrete. BF 50% concrete, especially, rather than BFS35%+FA15%, had a notable effect on resistance to chloride penetration and freezing/expansion. It has been confirmed that surface deterioration can be evaluated through a quantitative analysis of scaling, calculated from concrete's underwater weight and surface-dry weight as affected by the freezing-thawing of seawater.

• Journal of Advanced Marine Engineering and Technology
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• v.35 no.4
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• pp.373-378
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• 2011

The freeze desalination cycle with seawater heat pump system is simulated and designed for the basic data for the design of freeze desalination system. The basic model of seawater heat pump system is refrigeration cycle and indirect freeze desalination method is used for seawater desalination. The cycle performance of seawater heat pump such as COP, compressor work, condensing capacity was analyzed and the desalination performance such as fresh water productivity and energy per unit fresh water productivity was compared with respect to the seawater temperature of condenser inlet and ice ratio in the evaporator. The compressor work and condensing capacity decreased with respect to the decrease of seawater inlet temperature. The energy per unit fresh water productivity in case of $8^{\circ}C$ seawater inlet temperature showed 28.9% lower than that of $20^{\circ}C$.

• Journal of Microbiology
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• v.39 no.3
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• pp.240-243
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• 2001

The effect of cryoprotectants and suspending solutions on the preservation of marine heterotophic bacteria was investigated. Six halotolerant and four halophilic bacterial isolates suspended in either distilled water or artificial seawater were preserved in glycerol and dimethylsulfoxide at -70$\^{C}$, respectively. After one year of preservation, the recovery rates on the appropriate agar plates were estimated. The survival rate was found to be dependent on the strain tested, regardless of the preservation conditions tested.

• Proceedings of the Korean Institute of Building Construction Conference
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• 2009.11a
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• pp.43-46
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• 2009

The purpose of this study is to enhance durability of concrete structures that uses this surface protecting material by carrying out the performance test of the surface protecting material of concrete, and as s result, we reached out the conclusion as follow. 1. As a result of the test measuring the stability and adhesive power of conductive film against ultraviolet, freezing & thawing, and damage from seawater that deteriorate the surface protecting material, it was turned out to meet the performance criteria specifying in the KS standard enough to gain a good evaluation to use as a surface protecting material. 2. As a result of the test identifying the neutralization-furtherance, it was assessed to be capable of protecting effectively concrete structures from carbonic acid gas by a very low depth of 0.1mm of neutralization. 3. As a result of the test identifying Penetrated Resistance Properties of chloride ion, as it was turned out to have a very low value of 819 Coulombs, it was assessed that even in the environment where the corrosion by chloride such sea environment is very affective, the film can effectively protect the concrete structure. 4. As a result of the test identifying freezing & thawing, as there was no change in reduction of mass after 400 cycle, it was assessed that the film has a good resistance against freezing & thawing. According to the results of study above, it is expected that this technology can extend its durability of concrete structure and be widely used for concrete structure through means (methods) to prevent the neutralization and damage from seawater as original purposes of the surface protecting material.

• Journal of Korean Tunnelling and Underground Space Association
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• v.20 no.2
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• pp.255-268
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• 2018

Subsea tunnel can be highly vulnerable to seawater intrusion due to unexpected high-water pressure during construction. An artificial ground freezing (AGF) will be a promising alternative to conventional reinforcement or water-tightening technology under high-water pressure conditions. In this study, the freezing energy and required time was calculated by the theoretical model of the heat flow to estimate the total amount of refrigerant required for the artificial ground freezing. A lab-scale freezing chamber was devised to investigate changes in the thermal and mechanical properties of sandy soil corresponding to the variation of the salinity and water pressure. The freezing time was measured with different conditions during the chamber freezing tests. Its validity was evaluated by comparing the results between the freezing chamber experiment and the numerical analysis. In particular, the freezing time showed no significant difference between the theoretical model and the numerical analysis. The amount of refrigerant for artificial ground freezing was estimated from the numerical analysis and the freezing efficiency obtained from the chamber test. In addition, the energy ratio for maintaining frozen status was calculated by the proposed formula. It is believed that the energy ratio for freezing will depend on the depth of rock cover in the subsea tunnels and the water temperature on the sea floor.

• Journal of the korean society of oceanography
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• v.33 no.1-2
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• pp.1-7
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• 1998

In the western Weddell Sea, winter mixed layer is characterized by near-freezing temperature and higher salinity due to brine injection through sea-ice formation. This layer becomes Winter Water being capped by warmer and less saline Antarctic Surface Water during the sea-ice melt-ing season. In this study, Winter Water was preliminarily identified by the oxygen isotopic com-positions. The ${\delta}^{18}$O values of Winter Water show the progressively increasing trend from south to north in the study area. It presumably reflects the enhanced mixing with Antarctic Surface Water due to the extent of influence by low S'"0 value of sea-ice/glacier meltwater. Correlations between salinity and 6'"0 values of seawater can be used to more generally characterize Winter Water with a view to identification. However, the prediction on the degree of mixing from these relationships needs more detailed isotope data, although this study allows the oxygen isotopic composition of seawater as a tracer to identify the water mass.

• Journal of Forest and Environmental Science
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• v.30 no.1
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• pp.120-125
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• 2014

Halophytes are plants of saline habitats that grow under conditions that may vary in extremes of temperatures (freezing to very hot), water availability (drought to water logging) and salinity (mild to almost saturation). Halophytes may also face sudden micro-environmental variations within their habitats. In this study, we examine some of the factors that determine the ability of seeds of Artemisia fukudo to germinate when conditions are optimal for seedling growth and survival. Germination percentage was the highest at 77.5% when treated in 0% seawater and seedling growth was best in 0% seawater plug cell tray. Physiological quality (chlorophyll contents) and mean germination time were best in the condition in which seeds were treated with 0% seawater (control). Germination performance index in the control group showed the highest value to 3.8. Seedling growth, seedling vigor index in the length of seedling growth andthe relative growth rate were the highest values of 0.76 cm, 1.43, 0.0099, respectively.

• Food Science and Biotechnology
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• v.16 no.4
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• pp.659-662
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• 2007

In order to determine the optimal storage conditions of Capsosiphon fulvescens (maesaengi), 2 types of wash solutions (distilled water and seawater) and storage temperatures (-20 and $-80^{\circ}C$) were evaluated for the effectiveness of microbial growth inhibition and the changes of texture, color, and proximate composition. Thawed samples that had been washed with seawater and stored at $-20^{\circ}C$ for 50 days showed a 1.1-fold increase in hardness compared to the initial hardness of the sample ($1.9{\times}10^5\;dyne/cm^2$). There was no change in moisture, ash, or crude lipid during storage at -20 and $-80^{\circ}C$ for 60 days, while there was a $1{\pm}0.2%$ decrease in crude protein content for the control during storage at both -20 and $-80^{\circ}C$ for 60 days. In conclusion, the recommended optimal storage conditions for retaining the quality of C. fulvescens are: temperatures at or below $-20^{\circ}C$ and washings with either distilled water or seawater for inhibiting microbial growth, temperatures at or below $-20^{\circ}C$ and a washing with seawater to prevent reductions in hardness, and a temperature of $-80^{\circ}C$ and washings with either distilled water or seawater to protect against color changes.

• Proceedings of the Korean Society of Developmental Biology Conference
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• 2003.10a
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• pp.73-73
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• 2003

The present study examined the possibility of long term storage, by cryopreservation in liquid nitrogen, of the sperm of Filefish (Thamnaconus septentrionalis), and the changes in motility, survival rate and ultrastructure of the sperm after freezing and thawing. The sperm was collected by stripping and stored on ice until experiments. For selection of the immobilizing solution, diluted artificial seawater (ASW) of 20, 30 and 40% were tested. The sperm motility was significantly inhibited in 30% ASW, and restored entirely after 100% ASW was added again. Two cryoprotectants, dimethyl sulfoxide ($Me_2$SO) and glycerol, were added to 30% ASW to formulate the extenders at the concentrations between 5 to 20% by volume for freezing. The sperm was diluted at the ratio of 1 :6 with the extenders, inserted into 0.5ml plastic straws and frozen at a freezing rate of $50^{\circ}C$/min to $-100^{\circ}C$ after equilibration for 10 min at room temperature, followed by plunging into liquid nitrogen. The straws were thawed in a $30^{\circ}C$ water bath for 15 sec. The highest post-thawed sperm motility and survival rate were obtained with 5% glycerol Afterward, the effect of different freezing rates was examined using 5% glycerol as a cryoprotectant, and the rate of $20^{circ}C/min to $-80^{\circ}C$ showed the best result Some ultrastructural changes of sperm, such as the detachment of plasmatic and nuclear membranes, destruction of mitochondria, were observed after cryopreservation. Morphological normality of the sperm in 5% glycerol frozen at the ratio of 1$0^{\circ}C$/min to $-80^{\circ}C$ was better than that of others.